CuGenDBv2

Gene ID	CSPI01G03070
Organism	Cucumis sativus L. var. sativus cv. PI 183967 (Cucumber (PI 183967) v1)
Description	protein MIS12 homolog
Genome location	Chr1:1935268..1937214
RNA-Seq Expression	CSPI01G03070
Synteny	CSPI01G03070
Gene Ontology terms	GO:0000070 - mitotic sister chromatid segregation (biological process) GO:0034501 - protein localization to kinetochore (biological process) GO:0051301 - cell division (biological process) GO:0051382 - kinetochore assembly (biological process) GO:0000818 - nuclear MIS12/MIND complex (cellular component)
InterPro domains	IPR008685 - Centromere protein Mis12

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAA0058004.1 Centromere protein Mis12 [Cucumis melo var. makuwa]

9.1e-87

97.16

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTLGISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
        DESPGVTSI+HD DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ

KAA0058032.1 Centromere protein Mis12 [Cucumis melo var. makuwa]

2.6e-86

96.59

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTE SDRSQDLTLGISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
        DESPGVTSI+HD DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ

XP_004138204.3 protein MIS12 homolog isoform X1 [Cucumis sativus]

1.1e-89

99.43

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPS+
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
        DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ

XP_023515499.1 protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]

1.9e-76

85.96

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTE SDRSQDL+LGIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
        DESP VTS+S D  +DVDLDTELD LRNKLSEVRKEN+VLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MFQ
Subjt:  DESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ

XP_038879945.1 protein MIS12 homolog [Benincasa hispida]

2.0e-81

90.91

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Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAF FYQSQASA LKTE SDRSQDLTLGIS+VRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
        D+SPGVTSISHD+DV LDTELD LRNKLSEVRKENIVLNQELQALERQTA+SNSQI+HFNEALQLYEQ+SVN+MFQ
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LPL2 Uncharacterized protein

5.5e-90

99.43

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPS+
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
        DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ

A0A5A7UQ95 Centromere protein Mis12

1.3e-86

96.59

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTE SDRSQDLTLGISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
        DESPGVTSI+HD DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ

A0A5A7US57 Centromere protein Mis12

4.4e-87

97.16

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTLGISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
        DESPGVTSI+HD DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ

A0A6J1E0A1 protein MIS12 homolog

1.3e-75

85.39

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTE SDRSQDL LGIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
        DESP VTS+S D  +DVDLDTELD LRNKLSEVRKENIVLNQELQALERQ ASSNSQ++ FNEALQLYE++SVN+MFQ
Subjt:  DESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ

A0A6J1JD43 protein MIS12 homolog isoform X1

2.3e-75

85.39

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTE SDRSQDL LGIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
        DESP VTS+S D  +DVDLDTELD LR  LSEVRKENIVLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MFQ
Subjt:  DESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ

SwissProt top hits

e value

%identity

Alignment

Q2V0Z5 Protein MIS12 homolog

7.8e-41

50.56

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSD--RSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLP
        MEGSK E VF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK + SD  +SQ L+ GI++VR L+   +  RL +WE Y L  CF+VP+GF LP
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSD--RSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLP

Query:  SNDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
         ++ES   +S+  D  +D++LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+
Subjt:  SNDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ

Arabidopsis top hits

e value

%identity

Alignment

AT5G35520.1 minichromosome instability 12 (mis12)-like

5.6e-42

50.56

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSD--RSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLP
        MEGSK E VF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK + SD  +SQ L+ GI++VR L+   +  RL +WE Y L  CF+VP+GF LP
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSD--RSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLP

Query:  SNDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ
         ++ES   +S+  D  +D++LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+
Subjt:  SNDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQ

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGGAAGGAAGTAAAGGCGAAGTGGTGTTCAATTCACTCAATCTGAATCCACAGCTGTTCATTAATGAAGCTCTCAATACTGTCGACGATTTAGTTGATGATGCTTTCGA
TTTCTATCAATCACAAGCGTCGGCTGCCCTGAAGACCGAGAGCTCGGATAGATCTCAGGATCTTACCCTAGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGC
ATAAGCGATTGGCCATGTGGGAAAAGTACTGCCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAAATGATGAATCACCTGGTGTCACTTCAATAAGT
CATGACCATGACGTGGATCTGGATACAGAGCTTGATCTTTTGAGAAATAAGCTCTCCGAGGTCAGAAAAGAGAATATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAG
GCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGGTAACTAA

mRNA sequence

Show/hide mRNA sequence

CATTGGGTAAGAAGGTTTTTCAGGACGCAAAAGGGTAATTTTGATGGAAGGAAGTAAAGGCGAAGTGGTGTTCAATTCACTCAATCTGAATCCACAGCTGTTCATTAATG
AAGCTCTCAATACTGTCGACGATTTAGTTGATGATGCTTTCGATTTCTATCAATCACAAGCGTCGGCTGCCCTGAAGACCGAGAGCTCGGATAGATCTCAGGATCTTACC
CTAGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGCATAAGCGATTGGCCATGTGGGAAAAGTACTGCCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTC
TTTACCCTCAAATGATGAATCACCTGGTGTCACTTCAATAAGTCATGACCATGACGTGGATCTGGATACAGAGCTTGATCTTTTGAGAAATAAGCTCTCCGAGGTCAGAA
AAGAGAATATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGT
TCTGTGAATGACATGTTCCAGGGTAACTAAGTTGTGCTTAAAACCAATTTTTTCTTCCTGCACTCCTGGGTTTAC

Protein sequence

Show/hide protein sequence

MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNDESPGVTSIS
HDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQGN

CSPI01G03070 (gene) of Cucumber (PI 183967) v1 genome