CuGenDBv2

Gene ID	CSPI01G24410
Organism	Cucumis sativus L. var. sativus cv. PI 183967 (Cucumber (PI 183967) v1)
Description	PseudoU_synth_1 domain-containing protein
Genome location	Chr1:19700379..19700878
RNA-Seq Expression	CSPI01G24410
Synteny	CSPI01G24410
Gene Ontology terms	GO:0031119 - tRNA pseudouridine synthesis (biological process) GO:0003723 - RNA binding (molecular function) GO:0009982 - pseudouridine synthase activity (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

XP_004145230.1 putative tRNA pseudouridine synthase isoform X2 [Cucumis sativus]

2.5e-40

87.25

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Query:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF-SSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHP
        MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF SSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRG    +    L HP
Subjt:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF-SSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHP

Query:  LV
         +
Subjt:  LV

XP_008457308.1 PREDICTED: putative tRNA pseudouridine synthase [Cucumis melo]

3.5e-34

78.43

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Query:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSS-TASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHP
        MSVSSLRLPLSPWITK+SSSCFRFP PVFV RTP LRP LL+ FSS  ASTDAFST+CAPDQLRKWQAFRKKKVVLRIGYVGTDYRG    +    L HP
Subjt:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSS-TASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHP

Query:  LV
         +
Subjt:  LV

XP_031742713.1 putative tRNA pseudouridine synthase isoform X1 [Cucumis sativus]

1.1e-40

81.98

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Query:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF-SSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHP
        MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF SSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRG    +    L HP
Subjt:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF-SSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHP

Query:  LVNPSPFLINS
          N +  L ++
Subjt:  LVNPSPFLINS

XP_038894776.1 putative tRNA pseudouridine synthase isoform X3 [Benincasa hispida]

2.2e-28

80.46

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Query:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLH-FFSSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRG
        M+VSSLRLPLSPWITK SSSCF FP P FVSRT  L P L     SSTASTDAFST+CAP+QLRKW+AFRKKKVVLRIGYVGTDYRG
Subjt:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLH-FFSSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRG

XP_038894779.1 putative tRNA pseudouridine synthase isoform X5 [Benincasa hispida]

2.2e-28

80.46

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Query:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLH-FFSSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRG
        M+VSSLRLPLSPWITK SSSCF FP P FVSRT  L P L     SSTASTDAFST+CAP+QLRKW+AFRKKKVVLRIGYVGTDYRG
Subjt:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLH-FFSSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRG

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LVB6 Uncharacterized protein

3.4e-83

99.36

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Query:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF-SSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHP
        MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF SSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHP
Subjt:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFF-SSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHP

Query:  LVNPSPFLINSLVLTIQGYKCNATNCHIQVSFSLHYIRKIPIDGLTMASPEYSVAVL
        LVNPSPFLINSLVLTIQGYKCNATNCHIQVSFSLHYIRKIPIDGLTMASPEYSVAVL
Subjt:  LVNPSPFLINSLVLTIQGYKCNATNCHIQVSFSLHYIRKIPIDGLTMASPEYSVAVL

A0A1S3C5W1 putative tRNA pseudouridine synthase

1.7e-34

78.43

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Query:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSS-TASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHP
        MSVSSLRLPLSPWITK+SSSCFRFP PVFV RTP LRP LL+ FSS  ASTDAFST+CAPDQLRKWQAFRKKKVVLRIGYVGTDYRG    +    L HP
Subjt:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSS-TASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHP

Query:  LV
         +
Subjt:  LV

A0A6J1DMV6 putative tRNA pseudouridine synthase isoform X2

4.2e-25

74.71

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Query:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSS-TASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRG
        M+VSSLR PLSPW TK SS+CFR P   FVSR+P LRP LL   SS +ASTDA STRCAPDQLR W+AFRKKKVV+RIGY GTDYRG
Subjt:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSS-TASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRG

A0A6J1HM83 putative tRNA pseudouridine synthase

1.1e-25

74.71

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Query:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALL-HFFSSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRG
        M+VSSLR PLSPW  K S  CFRFP P FVS T  L P LL    SSTASTDA ST+CAPDQLRKW+AFRKKKVV+RIGYVGTDYRG
Subjt:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALL-HFFSSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRG

A0A6J1I156 putative tRNA pseudouridine synthase

5.9e-27

77.01

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Query:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALL-HFFSSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRG
        M+VSSLR PLSPW TK S  CFRFP P FVS T  LRP LL    SSTASTDA ST+CAPDQLRKW+AFRKKKVV+RIGYVGTDYRG
Subjt:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALL-HFFSSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRG

SwissProt top hits

e value

%identity

Alignment

O22928 Putative tRNA pseudouridine synthase

2.0e-08

39.05

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Query:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSTASTDAFSTRCAPDQ----LRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSL
        M+VS LR+P+        S  F +P     +R   + PALL F SS A   + ++   P        KW+++RKKKVV+RIGYVGTDYRG     L +  
Subjt:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSTASTDAFSTRCAPDQ----LRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSL

Query:  DHPLV
        D P +
Subjt:  DHPLV

Arabidopsis top hits

e value

%identity

Alignment

AT2G30320.1 Pseudouridine synthase family protein

1.4e-09

39.05

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Query:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSTASTDAFSTRCAPDQ----LRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSL
        M+VS LR+P+        S  F +P     +R   + PALL F SS A   + ++   P        KW+++RKKKVV+RIGYVGTDYRG     L +  
Subjt:  MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSTASTDAFSTRCAPDQ----LRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSL

Query:  DHPLV
        D P +
Subjt:  DHPLV

Sequences

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CDS sequence

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ATGTCAGTCTCTTCTTTGCGACTCCCACTTTCCCCATGGATAACCAAAGCTTCATCTTCATGTTTTCGTTTCCCCGACCCTGTTTTCGTTTCCCGGACTCCTCCTCTTAG
GCCTGCACTGCTCCACTTTTTTTCTTCTACTGCCTCCACCGATGCTTTTTCCACTCGGTGCGCTCCGGATCAGCTCCGGAAATGGCAAGCTTTCCGTAAGAAAAAGGTCG
TTTTGCGAATCGGCTATGTCGGGACTGATTATCGAGGTCCTCATTTCACGTTACTCTCTGTTTCTCTTGACCATCCTCTTGTGAATCCTTCACCATTTCTCATTAACTCA
CTAGTTTTGACAATTCAGGGCTACAAATGCAACGCAACCAACTGTCACATCCAAGTAAGTTTTTCTCTACATTATATCAGGAAAATTCCTATCGATGGATTGACCATGGC
ATCCCCTGAATATTCTGTTGCTGTGTTATGA

mRNA sequence

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GAATTCCCGCCCCCCAAGAATAGGGTGCAATGTCAGTCTCTTCTTTGCGACTCCCACTTTCCCCATGGATAACCAAAGCTTCATCTTCATGTTTTCGTTTCCCCGACCCT
GTTTTCGTTTCCCGGACTCCTCCTCTTAGGCCTGCACTGCTCCACTTTTTTTCTTCTACTGCCTCCACCGATGCTTTTTCCACTCGGTGCGCTCCGGATCAGCTCCGGAA
ATGGCAAGCTTTCCGTAAGAAAAAGGTCGTTTTGCGAATCGGCTATGTCGGGACTGATTATCGAGGTCCTCATTTCACGTTACTCTCTGTTTCTCTTGACCATCCTCTTG
TGAATCCTTCACCATTTCTCATTAACTCACTAGTTTTGACAATTCAGGGCTACAAATGCAACGCAACCAACTGTCACATCCAAGTAAGTTTTTCTCTACATTATATCAGG
AAAATTCCTATCGATGGATTGACCATGGCATCCCCTGAATATTCTGTTGCTGTGTTATGA

Protein sequence

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MSVSSLRLPLSPWITKASSSCFRFPDPVFVSRTPPLRPALLHFFSSTASTDAFSTRCAPDQLRKWQAFRKKKVVLRIGYVGTDYRGPHFTLLSVSLDHPLVNPSPFLINS
LVLTIQGYKCNATNCHIQVSFSLHYIRKIPIDGLTMASPEYSVAVL

CSPI01G24410 (gene) of Cucumber (PI 183967) v1 genome