CuGenDBv2

Gene ID	CSPI01G25270
Organism	Cucumis sativus L. var. sativus cv. PI 183967 (Cucumber (PI 183967) v1)
Description	twinkle homolog protein, chloroplastic/mitochondrial isoform X1
Genome location	Chr1:20731464..20740158
RNA-Seq Expression	CSPI01G25270
Synteny	CSPI01G25270
Gene Ontology terms	GO:0006260 - DNA replication (biological process) GO:0032508 - DNA duplex unwinding (biological process) GO:0003697 - single-stranded DNA binding (molecular function) GO:0005524 - ATP binding (molecular function) GO:0043139 - 5'-3' DNA helicase activity (molecular function)
InterPro domains	IPR006171 - TOPRIM domain IPR027032 - Twinkle-like protein IPR034154 - Archaeal primase DnaG/twinkle, TOPRIM domain

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

XP_011659237.2 twinkle homolog protein, chloroplastic/mitochondrial isoform X1 [Cucumis sativus]

5.3e-189

99.7

Show/hide

Query:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLG
        MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRP+SLVKPFAMKPNGVSSFTSHANVPRPPALLG
Subjt:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLG

Query:  NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI
        NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI
Subjt:  NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI

Query:  RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK
        RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK
Subjt:  RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK

Query:  LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
        LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
Subjt:  LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ

XP_011659240.2 twinkle homolog protein, chloroplastic/mitochondrial isoform X5 [Cucumis sativus]

5.3e-189

99.7

Show/hide

Query:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLG
        MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRP+SLVKPFAMKPNGVSSFTSHANVPRPPALLG
Subjt:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLG

Query:  NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI
        NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI
Subjt:  NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI

Query:  RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK
        RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK
Subjt:  RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK

Query:  LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
        LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
Subjt:  LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ

XP_031736080.1 twinkle homolog protein, chloroplastic/mitochondrial isoform X2 [Cucumis sativus]

5.3e-189

99.7

Show/hide

Query:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLG
        MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRP+SLVKPFAMKPNGVSSFTSHANVPRPPALLG
Subjt:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLG

Query:  NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI
        NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI
Subjt:  NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI

Query:  RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK
        RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK
Subjt:  RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK

Query:  LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
        LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
Subjt:  LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ

XP_031736081.1 twinkle homolog protein, chloroplastic/mitochondrial isoform X3 [Cucumis sativus]

5.3e-189

99.7

Show/hide

Query:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLG
        MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRP+SLVKPFAMKPNGVSSFTSHANVPRPPALLG
Subjt:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLG

Query:  NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI
        NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI
Subjt:  NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI

Query:  RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK
        RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK
Subjt:  RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK

Query:  LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
        LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
Subjt:  LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ

XP_031736083.1 twinkle homolog protein, chloroplastic/mitochondrial isoform X4 [Cucumis sativus]

5.3e-189

99.7

Show/hide

Query:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLG
        MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRP+SLVKPFAMKPNGVSSFTSHANVPRPPALLG
Subjt:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLG

Query:  NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI
        NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI
Subjt:  NPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNI

Query:  RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK
        RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK
Subjt:  RKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDK

Query:  LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
        LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
Subjt:  LSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LVL8 Toprim domain-containing protein

6.6e-145

99.22

Show/hide

Query:  MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGH
        MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGH
Subjt:  MKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGH

Query:  TLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEK
        TLAF DGRSSYK LGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEK
Subjt:  TLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEK

Query:  IFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQVTL
        IFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQVTL
Subjt:  IFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQVTL

A0A0A0LYM8 Uncharacterized protein

3.2e-171

91.89

Show/hide

Query:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHL---SSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPA
        MRFLH+NH LY PFSKLSSFSSP  LMGSFPLCKSTSLVFLSHL   SSS SQKYFLYRSISLLHGSFPVRP+SLVKPFAMKPNGVSSFTSHANVPRPPA
Subjt:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHL---SSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPA

Query:  LLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALK
        LL NPPDKAS STRLNILRKKLQDLDIDIEACVPGQF SLLCPMCKGGD++ERSFSLNISEDG AAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALK
Subjt:  LLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALK

Query:  QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGE
        QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRS+NQIAVAFTYYRGGALISCKYRDA K+FWQE  TE+IFYG+DDIDGASDIIIVEGE
Subjt:  QNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGE

Query:  IDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPA
        +DKLSMAEAGIHNCVSVPDGAP SVS+K+VPPA
Subjt:  IDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPA

A0A1S3BD14 twinkle homolog protein, chloroplastic/mitochondrial isoform X2

1.4e-158

Show/hide

Query:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHL------SSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPR
        MRFLHHN  LYNPFSKLSSFSSPF LMGSFPLCKSTSLVFLSHL      SSS+SQKYFLYRSISLLHGSFPVRP SLVKPFAMKPNG SSFTSHANVPR
Subjt:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHL------SSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPR

Query:  PPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSY------
        PPA   NP DKA  ST LNILRKKLQDL IDIEACVPGQFS LLCPMCKGGD+KERSF+L+ISEDG AAVWNCFRGKCGWKGHTLA  + RSSY      
Subjt:  PPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSY------

Query:  ---KDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDI
           +DLG+VALKQNIRKITVESLQLEPLCD++V YFAERLISK+TLLRNSVMQK   +QIA+AFTYYRGGAL+SCKYRD  K+FWQEA TEKIFYG+DDI
Subjt:  ---KDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDI

Query:  DGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
        +GASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKK+VP A Q
Subjt:  DGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ

A0A1S3BDI3 twinkle homolog protein, chloroplastic/mitochondrial isoform X1

1.4e-158

Show/hide

Query:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHL------SSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPR
        MRFLHHN  LYNPFSKLSSFSSPF LMGSFPLCKSTSLVFLSHL      SSS+SQKYFLYRSISLLHGSFPVRP SLVKPFAMKPNG SSFTSHANVPR
Subjt:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHL------SSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPR

Query:  PPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSY------
        PPA   NP DKA  ST LNILRKKLQDL IDIEACVPGQFS LLCPMCKGGD+KERSF+L+ISEDG AAVWNCFRGKCGWKGHTLA  + RSSY      
Subjt:  PPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSY------

Query:  ---KDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDI
           +DLG+VALKQNIRKITVESLQLEPLCD++V YFAERLISK+TLLRNSVMQK   +QIA+AFTYYRGGAL+SCKYRD  K+FWQEA TEKIFYG+DDI
Subjt:  ---KDLGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDI

Query:  DGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ
        +GASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKK+VP A Q
Subjt:  DGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQ

A0A6J1EMW1 twinkle homolog protein, chloroplastic/mitochondrial isoform X2

4.5e-141

76.99

Show/hide

Query:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHL-----SSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRP
        MRFLHHN  LY+ FSKLSS SS F LMGSF LCKS+SL+FLS L     SSS SQ++FLY+S S+LHGSFPVRPMS  K F+MKPNGVSSFTSHANVPRP
Subjt:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHL-----SSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRP

Query:  PALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVA
        P  + NP  +A   TRLNIL+KKLQ+LDID E CVPGQ + LLCPMCKGGD+ ER+ SL+ISEDG AAVW CFR KCGWKG TLAF DGRSS++  GQ+ 
Subjt:  PALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVA

Query:  LKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVE
        LKQ  RKITVESLQLEPLCDELV YFAERLISK TLLRNSVMQKRSNNQI++AFTY R GALISCKYRD  K+FWQEA TEKIFYGLDDIDGASDIIIVE
Subjt:  LKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVE

Query:  GEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQVT
        GEIDKLSMAEAG HNCVSVPDGAPPS S+K+VPP  Q T
Subjt:  GEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPAHQVT

SwissProt top hits

e value

%identity

Alignment

B5X582 Twinkle homolog protein, chloroplastic/mitochondrial

1.0e-70

48.8

Show/hide

Query:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSF-PV---RPMSLVKPFAMKPNGVSSFTSHANVPRPP
        MRFL    +++  F KLS   S   LMGS    +   L   S  S   S  Y   R +S +   F PV   RP+S   P+  + NG+SS+ S   VP  P
Subjt:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSF-PV---RPMSLVKPFAMKPNGVSSFTSHANVPRPP

Query:  ALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVAL
               DK  + +RL  LR+KL +  +D E C PGQ S L+CP C+GG++ E+S SL I+ DG +A WNCFRGKCG KG   A  DG  +  D     +
Subjt:  ALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVAL

Query:  KQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEG
        ++  RKITVE ++LEPLCDE+ DYFA R IS++TL RN VMQKR  ++I +AFTY++ G L+SCKYR   K F+QE KT +I YGLDDI+  S++IIVEG
Subjt:  KQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEG

Query:  EIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVP
        EIDKL+M EAG  NCVSVPDGAP  VS K +P
Subjt:  EIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVP

F4I6E6 Primase homolog protein

2.7e-58

50.77

Show/hide

Query:  MSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFR
        MS   P     NG SS  S   VP   A       K  + ++L  L +KL +  ID + C PG  S L+CP C+ GD+ E+S +L I  DG +A W C R
Subjt:  MSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFR

Query:  GKCGWKGHTLAFTDGRSSYKD-LGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKR
         KCG KG  +   DG+   KD +G+V      RKITVES++LEPLCDE+ D+FA R IS +TL RN VMQKR +++I +AFTY++ G L+SCKYR   K+
Subjt:  GKCGWKGHTLAFTDGRSSYKD-LGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKR

Query:  FWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVP
        F QE  T KI YGLDDI+  S+IIIVEGE DKL+M EAG  NCVSVPDGAP +VS K +P
Subjt:  FWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVP

Arabidopsis top hits

e value

%identity

Alignment

AT1G30660.1 nucleic acid binding;nucleic acid binding

1.9e-59

50.77

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Query:  MSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFR
        MS   P     NG SS  S   VP   A       K  + ++L  L +KL +  ID + C PG  S L+CP C+ GD+ E+S +L I  DG +A W C R
Subjt:  MSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFR

Query:  GKCGWKGHTLAFTDGRSSYKD-LGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKR
         KCG KG  +   DG+   KD +G+V      RKITVES++LEPLCDE+ D+FA R IS +TL RN VMQKR +++I +AFTY++ G L+SCKYR   K+
Subjt:  GKCGWKGHTLAFTDGRSSYKD-LGQVALKQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKR

Query:  FWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVP
        F QE  T KI YGLDDI+  S+IIIVEGE DKL+M EAG  NCVSVPDGAP +VS K +P
Subjt:  FWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVP

AT1G30680.1 toprim domain-containing protein

7.4e-72

48.8

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Query:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSF-PV---RPMSLVKPFAMKPNGVSSFTSHANVPRPP
        MRFL    +++  F KLS   S   LMGS    +   L   S  S   S  Y   R +S +   F PV   RP+S   P+  + NG+SS+ S   VP  P
Subjt:  MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSF-PV---RPMSLVKPFAMKPNGVSSFTSHANVPRPP

Query:  ALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVAL
               DK  + +RL  LR+KL +  +D E C PGQ S L+CP C+GG++ E+S SL I+ DG +A WNCFRGKCG KG   A  DG  +  D     +
Subjt:  ALLGNPPDKASISTRLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVAL

Query:  KQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEG
        ++  RKITVE ++LEPLCDE+ DYFA R IS++TL RN VMQKR  ++I +AFTY++ G L+SCKYR   K F+QE KT +I YGLDDI+  S++IIVEG
Subjt:  KQNIRKITVESLQLEPLCDELVDYFAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEG

Query:  EIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVP
        EIDKL+M EAG  NCVSVPDGAP  VS K +P
Subjt:  EIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVP

Sequences

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CDS sequence

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ATGCGTTTTCTTCATCACAATCACCGTCTCTATAACCCCTTTTCAAAACTCTCTTCATTTTCCTCACCATTTCGTCTAATGGGTTCCTTTCCTTTATGCAAATCAACCTC
TCTGGTTTTCCTGTCACACCTTTCTTCTTCTTTTTCGCAGAAGTATTTCCTGTACAGAAGCATTTCTCTTCTCCATGGTTCATTTCCTGTACGACCCATGTCCCTTGTGA
AACCCTTTGCCATGAAACCAAATGGGGTTTCTTCTTTTACTTCTCATGCCAATGTTCCCAGACCTCCAGCCCTTTTGGGGAATCCACCAGACAAGGCTTCAATCTCCACT
CGATTGAATATTCTAAGAAAAAAATTGCAGGATCTTGACATTGATATCGAAGCTTGTGTTCCTGGGCAGTTTTCCAGTTTGCTTTGTCCAATGTGTAAAGGTGGTGATAC
AAAGGAACGATCCTTCTCCCTAAATATCTCAGAAGATGGGGAGGCTGCTGTTTGGAATTGTTTTCGTGGAAAATGTGGTTGGAAAGGCCATACTCTGGCCTTTACTGATG
GTCGGTCATCATATAAAGATTTAGGACAAGTTGCACTTAAGCAGAACATACGGAAAATTACAGTGGAGAGTCTACAACTTGAACCACTGTGCGATGAGCTGGTTGATTAT
TTCGCGGAGCGATTGATATCCAAGCAAACATTGTTAAGAAATTCAGTTATGCAGAAAAGATCCAATAATCAGATTGCTGTTGCATTTACATATTATCGAGGTGGAGCATT
AATTAGTTGCAAGTATCGTGATGCCAAGAAAAGGTTCTGGCAGGAGGCCAAAACTGAGAAAATATTTTATGGATTGGATGACATAGATGGTGCAAGTGATATCATCATAG
TTGAAGGGGAGATAGACAAGCTCTCAATGGCAGAAGCTGGTATCCATAATTGCGTGAGTGTTCCTGATGGTGCACCACCATCAGTTTCCAAAAAAAACGTACCTCCTGCA
CATCAGGTTACACTTTGA

mRNA sequence

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GAATTTTATTTAGTAAAGAAAAAAAGGAGAAAAATCCTGCTAGTTGTGCAGGAAAGCCTCCCCCTTCCGTGATCGGGTTCTTTTGTCGTCGGAAGCAAGCCGCCGAAGAA
AGTTGGGGTGGTTTCCAGCAACCAAAACCCACTCTTCGCACACCCCTAACTCCTTCCTCTCTTTACCAATATTCATTTCAATGCCCCTGCCGGTTTCTTCAGTCTCTACC
TACCTACTATGTTATTTTTAACTCTGTAGTGTAGACTTGAAATGGTGGCCATACTCAGCTGATCACCTTAACCCTACCAATAGCCAGAATGCGTTTTCTTCATCACAATC
ACCGTCTCTATAACCCCTTTTCAAAACTCTCTTCATTTTCCTCACCATTTCGTCTAATGGGTTCCTTTCCTTTATGCAAATCAACCTCTCTGGTTTTCCTGTCACACCTT
TCTTCTTCTTTTTCGCAGAAGTATTTCCTGTACAGAAGCATTTCTCTTCTCCATGGTTCATTTCCTGTACGACCCATGTCCCTTGTGAAACCCTTTGCCATGAAACCAAA
TGGGGTTTCTTCTTTTACTTCTCATGCCAATGTTCCCAGACCTCCAGCCCTTTTGGGGAATCCACCAGACAAGGCTTCAATCTCCACTCGATTGAATATTCTAAGAAAAA
AATTGCAGGATCTTGACATTGATATCGAAGCTTGTGTTCCTGGGCAGTTTTCCAGTTTGCTTTGTCCAATGTGTAAAGGTGGTGATACAAAGGAACGATCCTTCTCCCTA
AATATCTCAGAAGATGGGGAGGCTGCTGTTTGGAATTGTTTTCGTGGAAAATGTGGTTGGAAAGGCCATACTCTGGCCTTTACTGATGGTCGGTCATCATATAAAGATTT
AGGACAAGTTGCACTTAAGCAGAACATACGGAAAATTACAGTGGAGAGTCTACAACTTGAACCACTGTGCGATGAGCTGGTTGATTATTTCGCGGAGCGATTGATATCCA
AGCAAACATTGTTAAGAAATTCAGTTATGCAGAAAAGATCCAATAATCAGATTGCTGTTGCATTTACATATTATCGAGGTGGAGCATTAATTAGTTGCAAGTATCGTGAT
GCCAAGAAAAGGTTCTGGCAGGAGGCCAAAACTGAGAAAATATTTTATGGATTGGATGACATAGATGGTGCAAGTGATATCATCATAGTTGAAGGGGAGATAGACAAGCT
CTCAATGGCAGAAGCTGGTATCCATAATTGCGTGAGTGTTCCTGATGGTGCACCACCATCAGTTTCCAAAAAAAACGTACCTCCTGCACATCAGGTTACACTTTGA

Protein sequence

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MRFLHHNHRLYNPFSKLSSFSSPFRLMGSFPLCKSTSLVFLSHLSSSFSQKYFLYRSISLLHGSFPVRPMSLVKPFAMKPNGVSSFTSHANVPRPPALLGNPPDKASIST
RLNILRKKLQDLDIDIEACVPGQFSSLLCPMCKGGDTKERSFSLNISEDGEAAVWNCFRGKCGWKGHTLAFTDGRSSYKDLGQVALKQNIRKITVESLQLEPLCDELVDY
FAERLISKQTLLRNSVMQKRSNNQIAVAFTYYRGGALISCKYRDAKKRFWQEAKTEKIFYGLDDIDGASDIIIVEGEIDKLSMAEAGIHNCVSVPDGAPPSVSKKNVPPA
HQVTL

CSPI01G25270 (gene) of Cucumber (PI 183967) v1 genome