CuGenDBv2

Gene ID	CSPI01G33100
Organism	Cucumis sativus L. var. sativus cv. PI 183967 (Cucumber (PI 183967) v1)
Description	DNA-directed RNA polymerase subunit beta
Genome location	Chr1:27995025..27998508
RNA-Seq Expression	CSPI01G33100
Synteny	CSPI01G33100
Gene Ontology terms	GO:0080093 - regulation of photorespiration (biological process) GO:0045454 - cell redox homeostasis (biological process) GO:0006351 - transcription, DNA-templated (biological process) GO:0006508 - proteolysis (biological process) GO:0009658 - chloroplast organization (biological process) GO:0045037 - protein import into chloroplast stroma (biological process) GO:0062091 - Ycf2/FtsHi complex (cellular component) GO:0009534 - chloroplast thylakoid (cellular component) GO:0009706 - chloroplast inner membrane (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0016887 - ATPase activity (molecular function) GO:0016464 - chloroplast protein-transporting ATPase activity (molecular function) GO:0005524 - ATP binding (molecular function) GO:0004222 - metalloendopeptidase activity (molecular function) GO:0004176 - ATP-dependent peptidase activity (molecular function) GO:0003899 - DNA-directed 5'-3' RNA polymerase activity (molecular function) GO:0003677 - DNA binding (molecular function)
InterPro domains	IPR037034 - RNA polymerase Rpb2, domain 2 superfamily IPR007642 - RNA polymerase Rpb2, domain 2

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

XP_004142587.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic [Cucumis sativus]

3.3e-50

100

Show/hide

Query:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
        KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
Subjt:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL

Query:  NPPLD
        NPPLD
Subjt:  NPPLD

XP_022158670.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic [Momordica charantia]

2.1e-44

89.52

Show/hide

Query:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
        KVAEKTALLRPVELK VP+ALE SAFRSK LDTDEL+ Y SWFATFSG+VP+W+QKTR VKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
Subjt:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL

Query:  NPPLD
        NPPLD
Subjt:  NPPLD

XP_023533094.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo]

9.4e-45

88.57

Show/hide

Query:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
        KVAEKT+LLRP+ELK VPLALE SAFR+KFLDTDEL+ YCSWFATF+G+VP+WVQKTR VK LNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
Subjt:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL

Query:  NPPLD
        NPPLD
Subjt:  NPPLD

XP_023533095.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo]

9.4e-45

88.57

Show/hide

Query:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
        KVAEKT+LLRP+ELK VPLALE SAFR+KFLDTDEL+ YCSWFATF+G+VP+WVQKTR VK LNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
Subjt:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL

Query:  NPPLD
        NPPLD
Subjt:  NPPLD

XP_038878867.1 probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic [Benincasa hispida]

1.5e-45

91.43

Show/hide

Query:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
        KVAEKTALLRPVELK VPLALE SAFRSKFLDTDEL+ Y SWFATFSG++P+WVQKTRIVKKLNKMLVNHLGL LSKEDLQNVVDLMEPYGQISNGIELL
Subjt:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL

Query:  NPPLD
        NPPLD
Subjt:  NPPLD

TrEMBL top hits

e value

%identity

Alignment

A0A0A0M3D4 RNA_pol_Rpb2_2 domain-containing protein

3.6e-98

85.71

Show/hide

Query:  PNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILA
        P+++  ++Y      KN  PK    + FYQQFSCVG          GDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILA
Subjt:  PNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRDILA

Query:  AADHLSGLKFGMVSTNQLKVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQN
        AADHL GLKFGMVSTNQLKVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQN
Subjt:  AADHLSGLKFGMVSTNQLKVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQN

Query:  VVDLMEPYGQISNGIELLNPPLDV
        VVDLMEPYGQISNGIELLNPPLDV
Subjt:  VVDLMEPYGQISNGIELLNPPLDV

A0A5B7C2I5 CARD domain-containing protein

1.7e-44

85.71

Show/hide

Query:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
        KVAEKTALLRP+ELK VP ALE SAFRSKFLDTDEL+SYCSWFATFSG+VP+W++KT+IVK ++KM+VNHLGLTL+KEDLQNVVDLMEPYGQISNGIELL
Subjt:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL

Query:  NPPLD
        NPPLD
Subjt:  NPPLD

A0A6J1DWH2 probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic

1.0e-44

89.52

Show/hide

Query:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
        KVAEKTALLRPVELK VP+ALE SAFRSK LDTDEL+ Y SWFATFSG+VP+W+QKTR VKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
Subjt:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL

Query:  NPPLD
        NPPLD
Subjt:  NPPLD

A0A6J1JID9 probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic isoform X2

3.0e-44

87.62

Show/hide

Query:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
        KVAEKT+LLRP+ELK VPLALE SAFR+KFLDTDEL+ YCSWFATF+G+VP+WV KTR VK LNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
Subjt:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL

Query:  NPPLD
        NPPLD
Subjt:  NPPLD

A0A6J1JKE3 probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic isoform X1

3.0e-44

87.62

Show/hide

Query:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
        KVAEKT+LLRP+ELK VPLALE SAFR+KFLDTDEL+ YCSWFATF+G+VP+WV KTR VK LNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
Subjt:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL

Query:  NPPLD
        NPPLD
Subjt:  NPPLD

SwissProt top hits

e value

%identity

Alignment

A6MMT6 DNA-directed RNA polymerase subunit beta

1.7e-36

65.57

Show/hide

Query:  ICNPNVETVNLYYPS-RLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQR
        +C P    + L +P+ + KK    KENA+LEFYQQF+CVG          GDPVFSESLCKELQKKFFQQRCELGRIGRRN+NQRLNLDIP+N+TFLL R
Subjt:  ICNPNVETVNLYYPS-RLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQR

Query:  DILAAADHLSGLKFGMVSTNQL
        D+LAAADHL G+KFGM + + L
Subjt:  DILAAADHLSGLKFGMVSTNQL

A9L988 DNA-directed RNA polymerase subunit beta

1.7e-36

69.57

Show/hide

Query:  ICNPNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRD
        +C P  E    +   + KK    KENAILEFYQQF+CVG          GDPVFSESLCKELQKKFFQQRCELGRIGRRN+NQRLNLDIP NNTFLL RD
Subjt:  ICNPNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRD

Query:  ILAAADHLSGLKFGM
        +LAAADHL G+KFGM
Subjt:  ILAAADHLSGLKFGM

F4J3N2 Probable inactive ATP-dependent zinc metalloprotease FTSHI 5, chloroplastic

2.3e-41

76.19

Show/hide

Query:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
        KV+EKT LLRP+ELK VP+ALE+SAFRSKFLDTDEL+SY SWFATFS +VP W++KT++ K + KMLVNHLGL L+K+DL+NVVDLMEPYGQISNGIELL
Subjt:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL

Query:  NPPLD
        NP +D
Subjt:  NPPLD

P60282 DNA-directed RNA polymerase subunit beta

3.4e-37

52.07

Show/hide

Query:  ICNPNVETVNLYYPS-RLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQR
        +C P    + LY+P+ + KK    KENAILEFYQQFSCVG          GDPVFSESLCKELQK+FFQQRCELGRIGR+N+NQRLN+DIP+NNTFLL R
Subjt:  ICNPNVETVNLYYPS-RLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQR

Query:  DILAAADHLSGLKFGMVSTNQLKVAEKTAL------------LRPVELKRVPLALEASAFRSKFLDTDE
        D+LAA DHL G+KFGM + + +   +   +            L  V L+ V       A R KF+ T +
Subjt:  DILAAADHLSGLKFGMVSTNQLKVAEKTAL------------LRPVELKRVPLALEASAFRSKFLDTDE

Q4VZP1 DNA-directed RNA polymerase subunit beta

1.0e-38

74.78

Show/hide

Query:  ICNPNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRD
        +C P  E    +   + KK    KENAILEFYQQFSCVG          GDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRD
Subjt:  ICNPNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRD

Query:  ILAAADHLSGLKFGM
        ILAAADHL GLKFGM
Subjt:  ILAAADHLSGLKFGM

Arabidopsis top hits

e value

%identity

Alignment

AT3G04340.1 FtsH extracellular protease family

1.6e-42

76.19

Show/hide

Query:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL
        KV+EKT LLRP+ELK VP+ALE+SAFRSKFLDTDEL+SY SWFATFS +VP W++KT++ K + KMLVNHLGL L+K+DL+NVVDLMEPYGQISNGIELL
Subjt:  KVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLTLSKEDLQNVVDLMEPYGQISNGIELL

Query:  NPPLD
        NP +D
Subjt:  NPPLD

ATCG00190.1 RNA polymerase subunit beta

1.9e-35

55.63

Show/hide

Query:  ICNPNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRD
        +C P  E    +   + KK    KENAILEFYQQFSCVG          GDP+FSESLCKELQKKFF QRCELGRIGRRN+N RLNL+IP+NN FLL RD
Subjt:  ICNPNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRLNLDIPENNTFLLQRD

Query:  ILAAADHLSGLKFGMVSTNQLKVAEKTALLRPVELKRVPLAL
        +LAAADHL G+KFGM + + +   +   +    +L +  L L
Subjt:  ILAAADHLSGLKFGMVSTNQLKVAEKTALLRPVELKRVPLAL

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGAAGATTGTCTCCTGGAACACTAGGGGTTTAAATGACTCTTCCAAATGTGCAGCCCTCAATAAGTTCATAAAAATCTGCAACCCTAATGTGGAGACTGTCAATCTTTA
TTATCCCTCTCGGCTGAAAAAAAACTGCCTCCCCAAAGAAAATGCCATTTTGGAGTTTTATCAACAATTTTCTTGTGTAGGCGGAGATCCGGTATTTTCTGAATCCTTAT
GCGGAGATCCGGTATTTTCTGAATCCTTATGTAAGGAATTACAAAAGAAATTCTTTCAACAAAGATGTGAATTAGGAAGGATTGGTCGACGAAATCTGAACCAGAGACTT
AATCTTGATATACCTGAGAACAATACATTTTTGTTACAGCGAGATATATTGGCAGCTGCGGATCATTTGAGTGGACTTAAATTTGGAATGGTATCTACTAATCAGTTAAA
AGTTGCTGAGAAGACGGCCCTTTTACGACCTGTGGAACTAAAGCGTGTCCCACTTGCCTTGGAAGCAAGTGCTTTTCGGAGCAAGTTCCTTGACACCGATGAACTGATTA
GCTACTGTAGCTGGTTTGCAACTTTCAGTGGTGTTGTTCCTGAGTGGGTGCAGAAAACCAGAATAGTAAAAAAACTAAACAAAATGTTGGTAAATCATCTGGGATTAACA
CTGTCTAAAGAAGATCTTCAAAATGTGGTTGATCTGATGGAACCATACGGTCAAATAAGCAATGGAATTGAACTTCTCAATCCTCCTCTTGATGTCTCTAAAGTTTAA

mRNA sequence

Show/hide mRNA sequence

Protein sequence

Show/hide protein sequence

MKIVSWNTRGLNDSSKCAALNKFIKICNPNVETVNLYYPSRLKKNCLPKENAILEFYQQFSCVGGDPVFSESLCGDPVFSESLCKELQKKFFQQRCELGRIGRRNLNQRL
NLDIPENNTFLLQRDILAAADHLSGLKFGMVSTNQLKVAEKTALLRPVELKRVPLALEASAFRSKFLDTDELISYCSWFATFSGVVPEWVQKTRIVKKLNKMLVNHLGLT
LSKEDLQNVVDLMEPYGQISNGIELLNPPLDVSKV

CSPI01G33100 (gene) of Cucumber (PI 183967) v1 genome