CuGenDBv2

Gene ID	CSPI02G24220
Organism	Cucumis sativus L. var. sativus cv. PI 183967 (Cucumber (PI 183967) v1)
Description	DNA-directed RNA polymerases I, II, and III subunit RPABC5
Genome location	Chr2:20766324..20767692
RNA-Seq Expression	CSPI02G24220
Synteny	CSPI02G24220
Gene Ontology terms	GO:0006360 - transcription by RNA polymerase I (biological process) GO:0006366 - transcription by RNA polymerase II (biological process) GO:0042797 - tRNA transcription by RNA polymerase III (biological process) GO:0005665 - RNA polymerase II, core complex (cellular component) GO:0005666 - RNA polymerase III complex (cellular component) GO:0005736 - RNA polymerase I complex (cellular component) GO:0003677 - DNA binding (molecular function) GO:0003899 - DNA-directed 5'-3' RNA polymerase activity (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	IPR000268 - DNA-directed RNA polymerase, subunit N/Rpb10 IPR020789 - RNA polymerases, subunit N, zinc binding site IPR023580 - RNA polymerase subunit RPB10

Homology

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GenBank top hits

e value

%identity

Alignment

AFK37774.1 unknown [Lotus japonicus]

4.0e-33

97.18

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
        MIIPVRCFTCGKVIGNKWD YLDLLQ+DYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

KAG6598222.1 DNA-directed RNA polymerase subunit 10-like protein, partial [Cucurbita argyrosperma subsp. sororia]

1.4e-33

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSD
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSD
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSD

XP_004138685.1 DNA-directed RNA polymerases I, II, and III subunit RPABC5 [Cucumis sativus]

1.2e-34

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

XP_022961651.1 DNA-directed RNA polymerase subunit 10-like protein [Cucurbita moschata]

6.1e-34

98.59

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSD S
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

XP_023536875.1 DNA-directed RNA polymerases I, II, and III subunit RPABC5 [Cucurbita pepo subsp. pepo]

1.0e-33

98.59

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
        MIIPVRCFTCGKVIGNKWD YLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LPW4 Uncharacterized protein

6.0e-35

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

A0A1S3C3L9 DNA-directed RNA polymerases I, II, and III subunit RPABC5

6.0e-35

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

A0A6J1BRM3 DNA-directed RNA polymerases I, II, and III subunit RPABC5

6.0e-35

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

A0A6J1GGE0 DNA-directed RNA polymerases I, II, and III subunit RPABC5

6.0e-35

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

A0A6J1KRF4 DNA-directed RNA polymerases I, II, and III subunit RPABC5

6.0e-35

100

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
        MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

SwissProt top hits

e value

%identity

Alignment

Q32P78 DNA-directed RNA polymerases I, II, and III subunit RPABC5

5.2e-28

80.6

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDK
        MIIPVRCFTCGK++GNKW+ YL LLQA+Y+EGDALDALGL RYCCRRML+ HVDLIEKLLNY  L+K
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDK

Q39290 DNA-directed RNA polymerases I, II, and III subunit RPABC5

1.1e-33

91.55

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
        MIIPVRCFTCGKVIGNKWD YLDLLQ DY+EGDALDAL LVRYCCRRMLMTHVDLIEKLLNYNTL+KSD S
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

Q6QN04 DNA-directed RNA polymerases I, II, and III subunit RPABC5

5.2e-28

80.6

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDK
        MIIPVRCFTCGK++GNKW+ YL LLQA+Y+EGDALDALGL RYCCRRML+ HVDLIEKLLNY  L+K
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDK

Q8LFJ6 DNA-directed RNA polymerases II, IV and V subunit 10

1.1e-33

91.55

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
        MIIPVRCFTCGKVIGNKWD YLDLLQ DY+EGDALDAL LVRYCCRRMLMTHVDLIEKLLNYNTL+KSD S
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

Q9SYA6 DNA-directed RNA polymerase subunit 10-like protein

1.1e-33

91.3

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSD
        MI+PVRCFTCGKVIGNKWD YL+LLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNT++KSD
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSD

Arabidopsis top hits

e value

%identity

Alignment

AT1G11475.1 RNA polymerases N / 8 kDa subunit

7.7e-35

91.55

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS
        MIIPVRCFTCGKVIGNKWD YLDLLQ DY+EGDALDAL LVRYCCRRMLMTHVDLIEKLLNYNTL+KSD S
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

AT1G61700.1 RNA polymerases N / 8 kDa subunit

7.7e-35

91.3

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Query:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSD
        MI+PVRCFTCGKVIGNKWD YL+LLQADY+EGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNT++KSD
Subjt:  MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSD

Sequences

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CDS sequence

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ATGATCATTCCAGTTCGTTGCTTCACTTGCGGAAAGGTGATTGGAAACAAATGGGATCATTACCTTGATCTTCTGCAGGCAGATTATTCAGAAGGTGATGCACTTGATGC
TTTGGGTTTGGTTCGCTACTGCTGTAGGCGAATGCTCATGACTCATGTTGACCTTATTGAGAAGCTTCTTAATTACAACACTCTGGATAAGTCTGATACCAGTTAG

mRNA sequence

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GTAAAAGGAATTTACCGTATAAATATATTGTTTTAAATAAACGCTGAAGAATATTGGAGAGGGGTTTGGGCAAAACCTCTCGGAACCCTAGAACGTGAAGTCGGAGGCAT
CGTCGGTCGGCAACAGAGGAGAGTGAAGATGATCATTCCAGTTCGTTGCTTCACTTGCGGAAAGGTGATTGGAAACAAATGGGATCATTACCTTGATCTTCTGCAGGCAG
ATTATTCAGAAGGTGATGCACTTGATGCTTTGGGTTTGGTTCGCTACTGCTGTAGGCGAATGCTCATGACTCATGTTGACCTTATTGAGAAGCTTCTTAATTACAACACT
CTGGATAAGTCTGATACCAGTTAGCCAAGGGACGAGTTTTCTGATTTTTCACGTGTAGGCGTATTGGCTTTCTGATGGTGTTTGTGTTCAGTTAAGAGTAAAGAAAAACT
ACCGGAGGGCTCGAGTTAAGCTATGTCTTGGCATTCTAGTTTGTTTAGTTATGGATTCATACGGACTCAAGTACTTGATCTTCTACGTGTTTGTTAGTATTTAGGAGTTG
TATATTAGAGCCGTGTAGCATTTTCCATAGATGCAGTTAATTTTCATTCAGGCAGCATTGACGTTTTTGTGGTGTTTGGATCCAGTTAAGAGAAAACAAAATTTATACGA
TCATGTAGTGCAGGATTTGATTTAAGCTATATCTTAGTGTCCTGGTTTATTTACTTATTGATTCATACGAACCCAAGTATTTAATCTTCTATGTGTTTATTAGGATTTAG
GAGTTATATGTAAGAGCCGTGTAGCATTTTCCATAGGATGCAGTCATTCAGTCAGTATTGACTTTTTTGTGGTGTTTGCATTCAGTTAAGAGAAAACAAGAACTATGGGA
ACATTCAGTTCAGGACTTGAGTTGAGCTATATATTGGTGCCCAGTTTGTTTGCTTATTGATTCATACGAGCCCAAGTATTTAGTCTTGTATGTGTTTATTAGTTTTTAGG
AGTAC

Protein sequence

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MIIPVRCFTCGKVIGNKWDHYLDLLQADYSEGDALDALGLVRYCCRRMLMTHVDLIEKLLNYNTLDKSDTS

CSPI02G24220 (gene) of Cucumber (PI 183967) v1 genome