CuGenDBv2

Gene ID	CSPI03G06910
Organism	Cucumis sativus L. var. sativus cv. PI 183967 (Cucumber (PI 183967) v1)
Description	SpoU_methylase domain-containing protein
Genome location	Chr3:6152672..6159135
RNA-Seq Expression	CSPI03G06910
Synteny	CSPI03G06910
Gene Ontology terms	GO:0001510 - RNA methylation (biological process) GO:0006396 - RNA processing (biological process) GO:0005794 - Golgi apparatus (cellular component) GO:0016020 - membrane (cellular component) GO:0003723 - RNA binding (molecular function) GO:0004491 - methylmalonate-semialdehyde dehydrogenase (acylating) activity (molecular function) GO:0008173 - RNA methyltransferase activity (molecular function)
InterPro domains	IPR029026 - tRNA (guanine-N1-)-methyltransferase, N-terminal IPR029028 - Alpha/beta knot methyltransferases IPR029064 - 50S ribosomal protein L30e-like

Homology

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GenBank top hits

e value

%identity

Alignment

XP_008450171.1 PREDICTED: uncharacterized tRNA/rRNA methyltransferase YsgA isoform X1 [Cucumis melo]

1.0e-101

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Query:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE
        MQCSCLSWSSNPKL P F KSKLSSIN AEAIDFTGRI LPSHVKAI+SISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHG+TCTLE
Subjt:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE

Query:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN
         LLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEK VDFKRWFPSPHRILVLE IQDPGNLGTLIRSALAFKWN
Subjt:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN

XP_011650605.1 uncharacterized protein LOC101218901 isoform X2 [Cucumis sativus]

1.5e-108

99.5

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Query:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE
        MQCSCLSWSSNPKLAP FPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE
Subjt:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE

Query:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN
        CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN
Subjt:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN

XP_016900854.1 PREDICTED: uncharacterized protein LOC103491842 isoform X2 [Cucumis melo]

5.2e-101

94.97

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Query:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE
        MQCSCLSWSSNPKL P F KSKLSSIN AEAIDFTGRI LPSHVKAI+SISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHG+TCTLE
Subjt:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE

Query:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKW
         LLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEK VDFKRWFPSPHRILVLE IQDPGNLGTLIRSALAFKW
Subjt:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKW

XP_031739071.1 uncharacterized protein LOC101218901 isoform X1 [Cucumis sativus]

6.3e-107

98.03

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Query:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPI---REICKFQESLHGVTC
        MQCSCLSWSSNPKLAP FPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPI   REICKFQESLHGVTC
Subjt:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPI---REICKFQESLHGVTC

Query:  TLECLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAF
        TLECLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAF
Subjt:  TLECLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAF

Query:  KWN
        KWN
Subjt:  KWN

XP_038904874.1 uncharacterized tRNA/rRNA methyltransferase slr1673 [Benincasa hispida]

8.8e-93

85.5

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Query:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE
        MQCSC+SWSSN KL P F KSKLSSIN AE IDF  R+ LPSHVKAITS SNPFVKHCVKLRFSSSYRH+HG+V+VVGATPIREICKFQESLHG+TCTLE
Subjt:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE

Query:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN
        CLLLLD+VEVPEGLV+ +VRIVRVS  VM+KLSG+QSTESIDAIALMRIP SFCSVDGNEK++DFKR FPSPHRILVLE IQDPGNLGTLIRSALAFKWN
Subjt:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN

TrEMBL top hits

e value

%identity

Alignment

A0A0A0L5K1 Uncharacterized protein

7.2e-109

94.84

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Query:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE
        MQCSCLSWSSNPKLAP FPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE
Subjt:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE

Query:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN
        CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN
Subjt:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN

Query:  DEEKKKLSELQEE
           +  ++ L EE
Subjt:  DEEKKKLSELQEE

A0A1S3BNM2 uncharacterized tRNA/rRNA methyltransferase YsgA isoform X1

5.0e-102

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Query:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE
        MQCSCLSWSSNPKL P F KSKLSSIN AEAIDFTGRI LPSHVKAI+SISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHG+TCTLE
Subjt:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE

Query:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN
         LLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEK VDFKRWFPSPHRILVLE IQDPGNLGTLIRSALAFKWN
Subjt:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN

A0A1S4DXY9 uncharacterized protein LOC103491842 isoform X2

2.5e-101

94.97

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Query:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE
        MQCSCLSWSSNPKL P F KSKLSSIN AEAIDFTGRI LPSHVKAI+SISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHG+TCTLE
Subjt:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE

Query:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKW
         LLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEK VDFKRWFPSPHRILVLE IQDPGNLGTLIRSALAFKW
Subjt:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKW

A0A5D3DF85 Putative tRNA/rRNA methyltransferase YsgA isoform X1

5.0e-102

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Query:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE
        MQCSCLSWSSNPKL P F KSKLSSIN AEAIDFTGRI LPSHVKAI+SISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHG+TCTLE
Subjt:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE

Query:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN
         LLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEK VDFKRWFPSPHRILVLE IQDPGNLGTLIRSALAFKWN
Subjt:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN

A0A6J1FNI0 uncharacterized protein LOC111447061

7.3e-85

80.5

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Query:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE
        MQCSC+S  SN KL P   K KLSSI   EAID   RI LPSHVKAITS SNPFVKHCVKLRF+SSYRHFHGSV+VVGATPIREICKFQESL G++CTLE
Subjt:  MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLE

Query:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN
        CLLLLDEVE+PE LV+++VR+VRVS  VMKKLSG+QSTESIDAIALMRIP SF S+DGN K+   KRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN
Subjt:  CLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT4G38020.1 tRNA/rRNA methyltransferase (SpoU) family protein

6.8e-59

64.85

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Query:  RIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLECLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQ
        R+ LPSHV +ITS +NPFVKHC+KLR SSSYRH HGSV+VVG  PIRE+C FQ +  G+T  +ECLLL +E ++P+GL +L++RIVRVSS VMKKLSG+Q
Subjt:  RIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLECLLLLDEVEVPEGLVNLTVRIVRVSSAVMKKLSGLQ

Query:  STESIDAIALMRIPPSFCSV-DGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN
        STES++AIALMRIP SF  + D  +   D  +WFPS HR+LVL+ IQDPGNLGTL+RSA+AF W+
Subjt:  STESIDAIALMRIPPSFCSV-DGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWN

Sequences

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CDS sequence

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ATGCAATGCTCCTGCCTTTCCTGGTCCTCAAATCCGAAACTCGCCCCTTTCTTTCCTAAATCGAAGCTATCATCAATTAACATAGCAGAAGCCATCGATTTTACTGGAAG
AATTCCTCTGCCTTCTCATGTTAAAGCCATCACCAGCATCTCCAACCCCTTCGTTAAGCACTGCGTCAAACTTCGTTTCAGTTCCTCTTATCGCCATTTCCATGGCTCCG
TCGTTGTTGTTGGAGCTACTCCAATAAGGGAAATTTGCAAGTTTCAAGAGTCTCTGCATGGTGTAACTTGTACATTGGAGTGCTTACTTCTGCTAGATGAGGTTGAGGTT
CCTGAAGGACTAGTTAATTTAACTGTTCGTATTGTGAGGGTGAGCTCAGCTGTGATGAAAAAACTTTCTGGCTTGCAATCAACAGAATCCATTGATGCAATTGCGCTCAT
GAGAATACCTCCCAGTTTTTGTAGTGTAGATGGCAATGAAAAAGAAGTAGATTTTAAAAGATGGTTTCCATCTCCACATCGAATATTAGTCCTTGAGGGAATCCAGGATC
CAGGCAATCTTGGTACGTTAATCAGGTCTGCTTTGGCCTTCAAATGGAATGATGAAGAAAAGAAGAAGCTTAGTGAATTGCAAGAGGAGCGCATGTTAAAGCTTTGCGAT
AGGAAGACTGCCTGGCGATTAACATCCCTAGGCGAAATGCCACATCATCACGCAATGAAGATTTACTAG

mRNA sequence

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GTAATTGAATCTCTAAAATCTATGCTGAGGTCACTTTGGCCCCTACATTTGAAGAGATTGCTATAGCACTAAACATTGAAACAAAAAGAAGCTGGGTGTATACTTAGGAT
TTGCAATGGTCTTTACAAAAAGAAGAAAAAAAATGAAAAAAATCCTGAAAAAAAAAACTGTGTTAGGCTGTGCCGGTGGAGTACGATAGAAGAATGCAATGCTCCTGCCT
TTCCTGGTCCTCAAATCCGAAACTCGCCCCTTTCTTTCCTAAATCGAAGCTATCATCAATTAACATAGCAGAAGCCATCGATTTTACTGGAAGAATTCCTCTGCCTTCTC
ATGTTAAAGCCATCACCAGCATCTCCAACCCCTTCGTTAAGCACTGCGTCAAACTTCGTTTCAGTTCCTCTTATCGCCATTTCCATGGCTCCGTCGTTGTTGTTGGAGCT
ACTCCAATAAGGGAAATTTGCAAGTTTCAAGAGTCTCTGCATGGTGTAACTTGTACATTGGAGTGCTTACTTCTGCTAGATGAGGTTGAGGTTCCTGAAGGACTAGTTAA
TTTAACTGTTCGTATTGTGAGGGTGAGCTCAGCTGTGATGAAAAAACTTTCTGGCTTGCAATCAACAGAATCCATTGATGCAATTGCGCTCATGAGAATACCTCCCAGTT
TTTGTAGTGTAGATGGCAATGAAAAAGAAGTAGATTTTAAAAGATGGTTTCCATCTCCACATCGAATATTAGTCCTTGAGGGAATCCAGGATCCAGGCAATCTTGGTACG
TTAATCAGGTCTGCTTTGGCCTTCAAATGGAATGATGAAGAAAAGAAGAAGCTTAGTGAATTGCAAGAGGAGCGCATGTTAAAGCTTTGCGATAGGAAGACTGCCTGGCG
ATTAACATCCCTAGGCGAAATGCCACATCATCACGCAATGAAGATTTACTAGAAGACAAGAATGATAGTTGGAGTTGATGTGACTTGACAAGATTGCAATTGGCGTTGAC
ATGTGCAGAAGACTAGAAGGTTTCCACCTAAAGTAGTCTATATAAAGCCTCCCCTCCTACCAAGTAAACTAGTCTGGTCTGAATGGATCAAACCCTAGAGAGCTCCATGT
GAGCCGGCGGAAAGAAATAGCCTTTCCCCTTTTGTAGAAGTTCTTCTCCTTCACCTCTTCTTCAGTCAGAAAGATTAGAGAGAGAAACCATCATCCTCTTCCCTCTAACT
TGTAAAAGAGCATGCTCGACTCCCCC

Protein sequence

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MQCSCLSWSSNPKLAPFFPKSKLSSINIAEAIDFTGRIPLPSHVKAITSISNPFVKHCVKLRFSSSYRHFHGSVVVVGATPIREICKFQESLHGVTCTLECLLLLDEVEV
PEGLVNLTVRIVRVSSAVMKKLSGLQSTESIDAIALMRIPPSFCSVDGNEKEVDFKRWFPSPHRILVLEGIQDPGNLGTLIRSALAFKWNDEEKKKLSELQEERMLKLCD
RKTAWRLTSLGEMPHHHAMKIY

CSPI03G06910 (gene) of Cucumber (PI 183967) v1 genome