CuGenDBv2

Gene ID	CSPI03G20910
Organism	Cucumis sativus L. var. sativus cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Valyl-tRNA synthetase
Genome location	Chr3:16966213..16967079
RNA-Seq Expression	CSPI03G20910
Synteny	CSPI03G20910
Gene Ontology terms	GO:0006438 - valyl-tRNA aminoacylation (biological process) GO:0106074 - aminoacyl-tRNA metabolism involved in translational fidelity (biological process) GO:0005737 - cytoplasm (cellular component) GO:0002161 - aminoacyl-tRNA editing activity (molecular function) GO:0004832 - valine-tRNA ligase activity (molecular function) GO:0005524 - ATP binding (molecular function)
InterPro domains	IPR002300 - Aminoacyl-tRNA synthetase, class Ia IPR002303 - Valine-tRNA ligase IPR009008 - Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6581186.1 Valine--tRNA ligase, chloroplastic/mitochondrial 2, partial [Cucurbita argyrosperma subsp. sororia]

2.1e-21

100

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Query:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Subjt:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

XP_011651387.1 valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucumis sativus]

5.5e-22

100

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Query:  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Subjt:  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

XP_011651390.1 valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucumis sativus]

5.5e-22

100

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Query:  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Subjt:  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

XP_022935323.1 valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X2 [Cucurbita moschata]

2.1e-21

100

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Query:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Subjt:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

XP_022983417.1 valine--tRNA ligase, chloroplastic/mitochondrial 2 isoform X1 [Cucurbita maxima]

2.1e-21

100

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Query:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Subjt:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

TrEMBL top hits

e value

%identity

Alignment

A0A5N6RLU1 Valyl-tRNA synthetase

3.0e-21

92.59

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Query:  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        SSDYLT+ATTRPETLFGDVAIAVHP+DDRYSKY+GMMAIVPMTYGRHVPIISD+
Subjt:  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

A0A6J1F580 Valyl-tRNA synthetase

1.0e-21

100

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Query:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Subjt:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

A0A6J1FA83 Valyl-tRNA synthetase

1.0e-21

100

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Query:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Subjt:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

A0A6J1IZ88 Valyl-tRNA synthetase

1.0e-21

100

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Query:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Subjt:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

A0A6J1J7Q1 Valyl-tRNA synthetase

1.0e-21

100

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Query:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
Subjt:  SDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

SwissProt top hits

e value

%identity

Alignment

A7HJX5 Valine--tRNA ligase

8.3e-13

60.78

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Query:  DYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISD
        DY+ +ATTRPET+ GD A+AVHP D+RY KYVG +AI+P+  GR +P+I+D
Subjt:  DYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISD

F4KE63 Valine--tRNA ligase, chloroplastic/mitochondrial 2

1.7e-21

85.19

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Query:  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        S D+LT+ATTRPETLFGDVA+AVHP+DDRYSKYVG  AIVPMTYGRHVPII+DK
Subjt:  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

Q7VQT2 Valine--tRNA ligase

3.2e-12

61.11

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Query:  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        ++DYLT+ATTRPET+ GDVAIAV+P+D RYS  +G     P+T  R +PIISDK
Subjt:  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

Q89AG3 Valine--tRNA ligase

1.9e-12

66.67

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Query:  YLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        YL +ATTRPETLFGD AIAVHP D RY KY+G  A+VP+   R +PIISD+
Subjt:  YLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

Q9HXH0 Valine--tRNA ligase

2.9e-13

63.46

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Query:  DYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        DYL VATTRPETL GD A+AVHP+D+RY+K +G  A +P+  GRH+PII+D+
Subjt:  DYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

Arabidopsis top hits

e value

%identity

Alignment

AT1G14610.1 valyl-tRNA synthetase / valine--tRNA ligase (VALRS)

1.8e-07

50.98

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Query:  VATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDKVTI
        VATTR ET+ GD AIA+HP D RY    G  A+ P   GR +PII D + +
Subjt:  VATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDKVTI

AT5G16715.1 ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases

1.2e-22

85.19

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Query:  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK
        S D+LT+ATTRPETLFGDVA+AVHP+DDRYSKYVG  AIVPMTYGRHVPII+DK
Subjt:  SSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDK

Sequences

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CDS sequence

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ATGGAAATGCCAGCTGGTGTGTCGGACACGTGTCAGAGGTGTCGGGGGTGTCAGTGTTGGACACGCTTGGCCATTTTAGGAGTGTTTGTGCTTCTTAGTAGTGATTACTT
GACAGTAGCAACAACTCGTCCTGAAACCTTATTTGGTGATGTAGCTATAGCAGTGCATCCTCAGGATGATCGTTATTCTAAATATGTTGGCATGATGGCAATTGTTCCAA
TGACGTATGGTCGTCATGTCCCTATCATCTCTGATAAGGTAACCATATTCTCAATTACCTTATCTATTTGA

mRNA sequence

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Protein sequence

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MEMPAGVSDTCQRCRGCQCWTRLAILGVFVLLSSDYLTVATTRPETLFGDVAIAVHPQDDRYSKYVGMMAIVPMTYGRHVPIISDKVTIFSITLSI

CSPI03G20910 (gene) of Cucumber (PI 183967) v1 genome