CuGenDBv2

Gene ID	CSPI03G24860
Organism	Cucumis sativus L. var. sativus cv. PI 183967 (Cucumber (PI 183967) v1)
Description	methyltransferase-like protein 25 isoform X5
Genome location	Chr3:22026497..22029252
RNA-Seq Expression	CSPI03G24860
Synteny	CSPI03G24860
Gene Ontology terms	GO:0032259 - methylation (biological process) GO:0008168 - methyltransferase activity (molecular function)
InterPro domains	IPR025714 - Methyltransferase domain IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAE8652020.1 hypothetical protein Csa_004742 [Cucumis sativus]

1.7e-111

95.93

Show/hide

Query:  MNVISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFH
        +++I  G   ENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASD+KSRAIVDVGAGQGYLAQALSFH
Subjt:  MNVISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFH

Query:  YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE
        YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE
Subjt:  YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE

Query:  EPSLVLAGLHACGDLSVIILR
        EPSLVLAGLHACGDLSVIILR
Subjt:  EPSLVLAGLHACGDLSVIILR

XP_011651525.1 protein RRNAD1 isoform X3 [Cucumis sativus]

1.7e-111

95.93

Show/hide

Query:  MNVISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFH
        +++I  G   ENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASD+KSRAIVDVGAGQGYLAQALSFH
Subjt:  MNVISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFH

Query:  YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE
        YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE
Subjt:  YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE

Query:  EPSLVLAGLHACGDLSVIILR
        EPSLVLAGLHACGDLSVIILR
Subjt:  EPSLVLAGLHACGDLSVIILR

XP_031737405.1 protein RRNAD1 isoform X2 [Cucumis sativus]

1.7e-111

95.93

Show/hide

Query:  MNVISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFH
        +++I  G   ENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASD+KSRAIVDVGAGQGYLAQALSFH
Subjt:  MNVISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFH

Query:  YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE
        YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE
Subjt:  YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE

Query:  EPSLVLAGLHACGDLSVIILR
        EPSLVLAGLHACGDLSVIILR
Subjt:  EPSLVLAGLHACGDLSVIILR

XP_031737406.1 protein RRNAD1 isoform X4 [Cucumis sativus]

1.7e-111

95.93

Show/hide

Query:  MNVISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFH
        +++I  G   ENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASD+KSRAIVDVGAGQGYLAQALSFH
Subjt:  MNVISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFH

Query:  YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE
        YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE
Subjt:  YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE

Query:  EPSLVLAGLHACGDLSVIILR
        EPSLVLAGLHACGDLSVIILR
Subjt:  EPSLVLAGLHACGDLSVIILR

XP_031737408.1 methyltransferase-like protein 25 isoform X6 [Cucumis sativus]

1.0e-111

95.07

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Query:  MNVISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFH
        +++I  G   ENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASD+KSRAIVDVGAGQGYLAQALSFH
Subjt:  MNVISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFH

Query:  YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE
        YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE
Subjt:  YKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDE

Query:  EPSLVLAGLHACGDLSVIILRLE
        EPSLVLAGLHACGDLSVIILR +
Subjt:  EPSLVLAGLHACGDLSVIILRLE

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LB00 Methyltranfer_dom domain-containing protein

2.2e-112

99.53

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Query:  ENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYKHSVLAIDA
        ENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASD+KSRAIVDVGAGQGYLAQALSFHYKHSVLAIDA
Subjt:  ENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYKHSVLAIDA

Query:  CSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDEEPSLVLAGLH
        CSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDEEPSLVLAGLH
Subjt:  CSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDEEPSLVLAGLH

Query:  ACGDLSVIILRLE
        ACGDLSVIILRLE
Subjt:  ACGDLSVIILRLE

A0A1S3B9Z8 methyltransferase-like protein 25 isoform X5

1.3e-104

90.99

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Query:  VISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYK
        +I  G   ENWP+SLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDL+S AIVDVGAGQGYLAQ LSFHYK
Subjt:  VISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYK

Query:  HSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAG---LEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSD
        HSVLAIDACSHHGNVTSARSARIKKYYL+QIRKAG   LEA+NLRLPKAMTFHVLSVDALKSLANMSLEDDHVEK S TGDDQKKINQQ+SKCLTLCNSD
Subjt:  HSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAG---LEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSD

Query:  EEPSLVLAGLHACGDLSVIILR
        +EPSLVLAGLHACGDLSVI+LR
Subjt:  EEPSLVLAGLHACGDLSVIILR

A0A1S3BAS0 protein RRNAD1 isoform X2

1.3e-104

90.99

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Query:  VISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYK
        +I  G   ENWP+SLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDL+S AIVDVGAGQGYLAQ LSFHYK
Subjt:  VISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYK

Query:  HSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAG---LEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSD
        HSVLAIDACSHHGNVTSARSARIKKYYL+QIRKAG   LEA+NLRLPKAMTFHVLSVDALKSLANMSLEDDHVEK S TGDDQKKINQQ+SKCLTLCNSD
Subjt:  HSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAG---LEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSD

Query:  EEPSLVLAGLHACGDLSVIILR
        +EPSLVLAGLHACGDLSVI+LR
Subjt:  EEPSLVLAGLHACGDLSVIILR

A0A1S3BAU0 protein RRNAD1 isoform X3

3.1e-106

92.24

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Query:  VISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYK
        +I  G   ENWP+SLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDL+S AIVDVGAGQGYLAQ LSFHYK
Subjt:  VISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYK

Query:  HSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDEEP
        HSVLAIDACSHHGNVTSARSARIKKYYL+QIRKAGLEA+NLRLPKAMTFHVLSVDALKSLANMSLEDDHVEK S TGDDQKKINQQ+SKCLTLCNSD+EP
Subjt:  HSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDEEP

Query:  SLVLAGLHACGDLSVIILR
        SLVLAGLHACGDLSVI+LR
Subjt:  SLVLAGLHACGDLSVIILR

A0A1S4DV55 protein RRNAD1 isoform X1

1.3e-104

90.99

Show/hide

Query:  VISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYK
        +I  G   ENWP+SLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDL+S AIVDVGAGQGYLAQ LSFHYK
Subjt:  VISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYK

Query:  HSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAG---LEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSD
        HSVLAIDACSHHGNVTSARSARIKKYYL+QIRKAG   LEA+NLRLPKAMTFHVLSVDALKSLANMSLEDDHVEK S TGDDQKKINQQ+SKCLTLCNSD
Subjt:  HSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAG---LEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSD

Query:  EEPSLVLAGLHACGDLSVIILR
        +EPSLVLAGLHACGDLSVI+LR
Subjt:  EEPSLVLAGLHACGDLSVIILR

SwissProt top hits

e value

%identity

Alignment

Q6AYG0 Protein RRNAD1

9.6e-04

24.65

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Query:  WPDSLKKFIRTSKSLAFQRE---QADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYKHSVLAID
        WP +L     T+ +LAF R        + +      + L     + +  KK+HE+  L  ++  ++       +VDVG+GQG+L++ +S       L + 
Subjt:  WPDSLKKFIRTSKSLAFQRE---QADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYKHSVLAID

Query:  ACSHHGNV-TSARSARIKKYYLSQIRKAGLEAKNL--RLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDEEPSLVL
          S  GN    AR+ R+ +  L  + K       +  R P+    HV     ++ ++  +L ++ +    M G    +                   L+L
Subjt:  ACSHHGNV-TSARSARIKKYYLSQIRKAGLEAKNL--RLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDEEPSLVL

Query:  AGLHACGDLSVIILR
         GLHACGDLSV +LR
Subjt:  AGLHACGDLSVIILR

Q6NXH8 Methyltransferase-like protein 25

1.2e-06

37.84

Show/hide

Query:  MNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYKHSVLAIDACSHHGNVTSARSARIKKYY
        MN KK HEV+ +S +I  IA     + I+DVG+G+GYL+  LS  Y  +V  ID+ + + +    R+ ++KK++
Subjt:  MNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYKHSVLAIDACSHHGNVTSARSARIKKYY

Q8N6Q8 Methyltransferase-like protein 25

4.2e-07

33.33

Show/hide

Query:  MNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKA
        MN KK HEV+ +S +IS IA     + ++D+G+G+GYL+  LS  Y   V  ID+ + + +    R+ ++KK++     ++ L+   L L  A
Subjt:  MNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYKHSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKA

Arabidopsis top hits

e value

%identity

Alignment

AT1G16650.1 S-adenosyl-L-methionine-dependent methyltransferases superfamily protein

1.6e-54

51.6

Show/hide

Query:  VISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYK
        +I  G   ++WP SLKKF+ T +SL+F REQADLQ +L    M  L+TVLSQGMN KKKHEVEVLS+++S +   + +  +VDVGAGQGYLAQ LSF YK
Subjt:  VISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYK

Query:  HSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDEEP
        HSV+AID+ SHHG VT AR+ARIKK++ +Q+RK+G   K   +P  +T  VLS + LK+L ++ LE D  + +    +++ +   Q S      +++   
Subjt:  HSVLAIDACSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDEEP

Query:  SLVLAGLHACGDLSVIILR
        SLVLAGLHACGDLSV +LR
Subjt:  SLVLAGLHACGDLSVIILR

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGAATGTTATTAGCATTGGCAACAAGGGAGAAAACTGGCCAGATTCGTTAAAGAAATTTATCCGTACTTCAAAATCTCTTGCCTTTCAACGTGAACAAGCAGACTTGCA
GATGGTCCTGCCTGGCTGGTGCATGGCTTCGCTTAACACTGTTCTTTCTCAAGGCATGAATCAGAAGAAAAAACATGAAGTTGAAGTCCTTTCCGCCATTATTAGTTTAA
TTGCAAGTGACCTGAAAAGTCGTGCAATTGTTGATGTGGGTGCTGGTCAGGGTTACTTAGCACAAGCGCTCTCCTTCCATTACAAGCATTCAGTTCTTGCAATTGATGCC
TGTTCCCATCATGGAAATGTCACAAGTGCACGTTCAGCACGGATTAAGAAGTATTACTTATCTCAAATTCGTAAAGCTGGGTTGGAAGCCAAAAATTTGAGACTGCCAAA
GGCAATGACATTCCATGTGTTGTCCGTTGACGCATTAAAATCCCTTGCTAACATGTCGCTAGAAGACGATCATGTTGAGAAGACAAGTATGACCGGTGATGATCAAAAGA
AAATAAACCAACAGGATTCGAAGTGTTTGACTTTGTGCAATTCTGACGAAGAGCCTTCATTGGTTCTTGCTGGTCTTCATGCTTGTGGTGATCTTTCGGTGATAATTCTT
AGGTTAGAATAG

mRNA sequence

Show/hide mRNA sequence

Protein sequence

Show/hide protein sequence

MNVISIGNKGENWPDSLKKFIRTSKSLAFQREQADLQMVLPGWCMASLNTVLSQGMNQKKKHEVEVLSAIISLIASDLKSRAIVDVGAGQGYLAQALSFHYKHSVLAIDA
CSHHGNVTSARSARIKKYYLSQIRKAGLEAKNLRLPKAMTFHVLSVDALKSLANMSLEDDHVEKTSMTGDDQKKINQQDSKCLTLCNSDEEPSLVLAGLHACGDLSVIIL
RLE

CSPI03G24860 (gene) of Cucumber (PI 183967) v1 genome