CuGenDBv2

Gene ID	CSPI03G35600
Organism	Cucumis sativus L. var. sativus cv. PI 183967 (Cucumber (PI 183967) v1)
Description	mediator of RNA polymerase II transcription subunit 27
Genome location	Chr3:31010055..31014640
RNA-Seq Expression	CSPI03G35600
Synteny	CSPI03G35600
Gene Ontology terms	GO:0006357 - regulation of transcription by RNA polymerase II (biological process) GO:0045893 - positive regulation of transcription, DNA-templated (biological process) GO:0016592 - mediator complex (cellular component) GO:0003713 - transcription coactivator activity (molecular function)
InterPro domains	IPR021627 - Mediator complex, subunit Med27

Homology

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GenBank top hits

e value

%identity

Alignment

XP_004136512.1 mediator of RNA polymerase II transcription subunit 27 isoform X1 [Cucumis sativus]

1.4e-239

100

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Query:  MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEE
        MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEE
Subjt:  MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEE

Query:  SGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSR
        SGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSR
Subjt:  SGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSR

Query:  SLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS
        SLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS
Subjt:  SLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS

Query:  PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITS
        PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITS
Subjt:  PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITS

Query:  LKDQKLDSVQAFHIDCFSEEI
        LKDQKLDSVQAFHIDCFSEEI
Subjt:  LKDQKLDSVQAFHIDCFSEEI

XP_008442914.1 PREDICTED: mediator of RNA polymerase II transcription subunit 27 [Cucumis melo]

1.6e-235

98.1

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Query:  MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEE
        MRHIQQPSQGQTATVSP+THSP S DDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEE
Subjt:  MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEE

Query:  SGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSR
        SGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSR
Subjt:  SGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSR

Query:  SLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS
        SLSDILSC+EKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHP+GSVEPDALAFFS
Subjt:  SLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS

Query:  PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITS
        PDERGSHVHARGFSNYH+FRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPP VRPYKQFCASKNSS VPITS
Subjt:  PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITS

Query:  LKDQKLDSVQAFHIDCFSEEI
        LKDQKLDSVQAFHIDCFSEEI
Subjt:  LKDQKLDSVQAFHIDCFSEEI

XP_022940393.1 mediator of RNA polymerase II transcription subunit 27 [Cucurbita moschata]

3.6e-211

89.34

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Query:  MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQ
        MR  Q P+QG TATVSP+T   HS  S ++APPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELI
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL++EN SN EPA KKPCFSHGVTDVKQ EL 
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELI

Query:  DSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA
        DS SLSDIL+CMEKEVP+LKIFTYERLDWLKQASALPSQANENSIGALKEH+YHSPRMSP +L AT  DKA VIELIIPSVFRAVVSLHPVGSVEPDA+A
Subjt:  DSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA

Query:  FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP
        FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQYF+G Q KAAL+PLLLWICSYQNLFSKACSKCGR LSVNKQSDLLLPPA+RPYKQFC SK SSVVP
Subjt:  FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP

Query:  ITSLKDQKLDSVQAFHIDCFSE
        +TSLKDQKLDSVQAFHI CF+E
Subjt:  ITSLKDQKLDSVQAFHIDCFSE

XP_023539656.1 mediator of RNA polymerase II transcription subunit 27 [Cucurbita pepo subsp. pepo]

9.0e-210

88.86

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Query:  MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQ
        MR  Q P+QG TATVSP+T   HS  S ++APPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELI
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL+DEN SN EPA KKPCFSHGVTDVKQ EL 
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELI

Query:  DSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA
        DS SLSDIL+C+EKEVP+LKIFTYERLDWLKQASAL SQANENSIGALKEH+YHSPRMSP +L AT  DKA VIELIIPSVFRAVVSLHPVGSVEPDA+A
Subjt:  DSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA

Query:  FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP
        FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQYF+G Q KAAL+PLLLWICSYQNLFSKACSKCGR LSVNKQSDLLLPPA+RPYKQFC SK SS VP
Subjt:  FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP

Query:  ITSLKDQKLDSVQAFHIDCFSE
        +TSLKDQKLDSVQAFHI CF+E
Subjt:  ITSLKDQKLDSVQAFHIDCFSE

XP_038904949.1 mediator of RNA polymerase II transcription subunit 27 [Benincasa hispida]

3.4e-225

93.87

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Query:  MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQ
        MR  QQP+QGQTATVSP+T   HSP S DDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELI
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDEN SNNEPALKKPCFSHGVTDVKQNEL 
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELI

Query:  DSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA
        DSRSLS+IL+CMEKEVPELKIFTYERLDWLKQASALPSQANENSIG LKEHSYHSPRMSPG+LGATP DKAGVIEL+IPSVFRAVVSLHPVGSVEPDA+A
Subjt:  DSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA

Query:  FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP
        FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQYFLGNQ KAALYPLLLWICSYQNLFSKACSKCG+ LSVNKQSDLLLPPAVRPYKQFCASKNSS VP
Subjt:  FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP

Query:  ITSLKDQKLDSVQAFHIDCFSEEI
        ITSLKDQKLDSVQAFHIDCFS E+
Subjt:  ITSLKDQKLDSVQAFHIDCFSEEI

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LGT1 Uncharacterized protein

6.9e-240

100

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Query:  MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEE
        MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEE
Subjt:  MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEE

Query:  SGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSR
        SGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSR
Subjt:  SGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSR

Query:  SLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS
        SLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS
Subjt:  SLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS

Query:  PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITS
        PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITS
Subjt:  PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITS

Query:  LKDQKLDSVQAFHIDCFSEEI
        LKDQKLDSVQAFHIDCFSEEI
Subjt:  LKDQKLDSVQAFHIDCFSEEI

A0A1S3B7L6 mediator of RNA polymerase II transcription subunit 27

7.9e-236

98.1

Show/hide

Query:  MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEE
        MRHIQQPSQGQTATVSP+THSP S DDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEE
Subjt:  MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEE

Query:  SGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSR
        SGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSR
Subjt:  SGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSR

Query:  SLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS
        SLSDILSC+EKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHP+GSVEPDALAFFS
Subjt:  SLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFS

Query:  PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITS
        PDERGSHVHARGFSNYH+FRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPP VRPYKQFCASKNSS VPITS
Subjt:  PDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITS

Query:  LKDQKLDSVQAFHIDCFSEEI
        LKDQKLDSVQAFHIDCFSEEI
Subjt:  LKDQKLDSVQAFHIDCFSEEI

A0A6J1DC62 mediator of RNA polymerase II transcription subunit 27

1.4e-203

85.38

Show/hide

Query:  MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQ
        MR  QQP+QGQTATVSP+T   HS  S ++APPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTA PHQS+KPLHLFQKED SMRQHL DLRAVGKQ
Subjt:  MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELI
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL+DE+  N EPALKKPCFSHGVT++KQ+EL 
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELI

Query:  DSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA
        + R+LSDIL+CMEKEVP+LKIFTYERLDWLKQA AL SQAN+NSIGALKEH+YHSPRM PG+L A+  DK  VIELIIPSVFRAVVSLHPVGSVEPDA+A
Subjt:  DSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA

Query:  FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP
        FF+PDERGS+VHARGFS +HLFRHVTEHAATVLQYFLG Q KAALYPLLLWICSYQNLFSKACSKCGR LSVNKQSDLLLPPA+RPYKQFC S+  S VP
Subjt:  FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP

Query:  ITSLKDQKLDSVQAFHIDCFSEEI
        I+SLKDQ+ D VQAFHI CFSEE+
Subjt:  ITSLKDQKLDSVQAFHIDCFSEEI

A0A6J1FP59 mediator of RNA polymerase II transcription subunit 27

1.8e-211

89.34

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Query:  MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQ
        MR  Q P+QG TATVSP+T   HS  S ++APPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELI
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL++EN SN EPA KKPCFSHGVTDVKQ EL 
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELI

Query:  DSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA
        DS SLSDIL+CMEKEVP+LKIFTYERLDWLKQASALPSQANENSIGALKEH+YHSPRMSP +L AT  DKA VIELIIPSVFRAVVSLHPVGSVEPDA+A
Subjt:  DSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA

Query:  FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP
        FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQYF+G Q KAAL+PLLLWICSYQNLFSKACSKCGR LSVNKQSDLLLPPA+RPYKQFC SK SSVVP
Subjt:  FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP

Query:  ITSLKDQKLDSVQAFHIDCFSE
        +TSLKDQKLDSVQAFHI CF+E
Subjt:  ITSLKDQKLDSVQAFHIDCFSE

A0A6J1KZZ9 mediator of RNA polymerase II transcription subunit 27

4.3e-210

88.63

Show/hide

Query:  MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQ
        MR  Q P+QG TATVSP+T   HS  S ++APPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQS+KPLHLFQKEDASMRQHLLDLRAVGKQ
Subjt:  MRHIQQPSQGQTATVSPST---HSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQ

Query:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELI
        LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHL++EN SN EPA KKPCFSHGVTDVKQ EL 
Subjt:  LEESGVLSESLLSRSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELI

Query:  DSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA
        DS SLSDIL+CMEKEVP+LKIFTYERLDWLKQAS LPSQANENSIG LKEH+YHSPRMSP +L AT  DKA VIELIIPSVFRAVVSLHPVGSVEPDA+A
Subjt:  DSRSLSDILSCMEKEVPELKIFTYERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALA

Query:  FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP
        FFSPDERGSHVHARGFSN+HLFRHVTEHAATVLQYF+G Q KAAL+PLLLWICSYQNLFSKACSKCGR LSVNKQSDLLLPPA+RPYKQFC SK SS VP
Subjt:  FFSPDERGSHVHARGFSNYHLFRHVTEHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVP

Query:  ITSLKDQKLDSVQAFHIDCFSE
        +TSLKDQKLDSVQAFHI CF+E
Subjt:  ITSLKDQKLDSVQAFHIDCFSE

SwissProt top hits

e value

%identity

Alignment

Q8RWM3 Mediator of RNA polymerase II transcription subunit 27

2.7e-124

58.94

Show/hide

Query:  SPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMPLVC
        S  DAPPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V +QP  +  PL LF +EDASMRQHL DLR +GK+LEESGVL+ESL SRSNSWGLHMPLVC
Subjt:  SPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMPLVC

Query:  PDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYER
        PDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPH++D      EP+ KK   SH + +  + E +D ++L DI S +EK VP +K+ TY R
Subjt:  PDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYER

Query:  LDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFSPDERGSHVHARGFSNYHLFRHVT
        L+WLK+A++LP   +++   A K     S ++  G L     DK  VIEL  PS+FRA+VSL P GSV+PDA+AFFSPDE GS++HARGFS YH+++H+T
Subjt:  LDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFSPDERGSHVHARGFSNYHLFRHVT

Query:  EHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITSLKDQKLDSVQAFHIDCFSEE
        EHAAT LQYFLG     ALY LLLWICS++++FSK C+KCGR L+++K+S L+LPP  R Y++   + N             LD  +A+H  C  ++
Subjt:  EHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITSLKDQKLDSVQAFHIDCFSEE

Arabidopsis top hits

e value

%identity

Alignment

AT3G09180.1 CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med27 (InterPro:IPR021627); Has 112 Blast hits to 112 proteins in 38 species: Archae - 0; Bacteria - 0; Metazoa - 79; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink).

1.9e-125

58.94

Show/hide

Query:  SPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMPLVC
        S  DAPPKQVA AM+RL  AAR+IADIRLGADR+LEA+ V +QP  +  PL LF +EDASMRQHL DLR +GK+LEESGVL+ESL SRSNSWGLHMPLVC
Subjt:  SPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLSRSNSWGLHMPLVC

Query:  PDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYER
        PDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPH++D      EP+ KK   SH + +  + E +D ++L DI S +EK VP +K+ TY R
Subjt:  PDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFTYER

Query:  LDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFSPDERGSHVHARGFSNYHLFRHVT
        L+WLK+A++LP   +++   A K     S ++  G L     DK  VIEL  PS+FRA+VSL P GSV+PDA+AFFSPDE GS++HARGFS YH+++H+T
Subjt:  LDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFSPDERGSHVHARGFSNYHLFRHVT

Query:  EHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITSLKDQKLDSVQAFHIDCFSEE
        EHAAT LQYFLG     ALY LLLWICS++++FSK C+KCGR L+++K+S L+LPP  R Y++   + N             LD  +A+H  C  ++
Subjt:  EHAATVLQYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITSLKDQKLDSVQAFHIDCFSEE

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGCGACATATTCAGCAGCCATCGCAGGGCCAAACTGCGACGGTCAGCCCTAGCACTCACTCTCCCCCCTCCCCCGACGACGCTCCTCCCAAGCAGGTTGCTCTCGCCAT
GGACAGACTCGGCCACGCCGCTCGTCTCATCGCCGACATCAGACTCGGCGCTGATCGCCTTCTCGAAGCCCTATGCGTCACTGCTCAGCCTCATCAAAGCTCTAAGCCCC
TCCATTTGTTTCAGAAAGAAGACGCTTCCATGCGCCAACACCTCCTCGACCTTCGTGCAGTTGGAAAGCAGCTTGAAGAGTCTGGCGTTCTCAGTGAATCTCTTCTTTCG
AGAAGTAACTCTTGGGGTCTACATATGCCTTTAGTGTGTCCTGATGGCGCTGTGGTTGCTTATGCTTGGAAACGTCAATTGGCTGGCCAGGCTGGTGCATCTGCAGTTGA
CCGAACAAGGTTAGCTTTAAAGGCCTTCACTGACCAAAAAAGACGGTTTTTCCCTCATCTTGAAGATGAAAATGCGTCAAATAATGAGCCTGCTCTGAAGAAGCCTTGTT
TTTCTCATGGAGTGACAGATGTCAAACAAAATGAGCTTATTGATTCTAGATCACTCTCGGATATTTTATCCTGTATGGAAAAGGAAGTGCCAGAGCTTAAGATTTTCACT
TATGAGCGATTGGACTGGTTAAAACAAGCTTCGGCCCTTCCCTCTCAAGCAAATGAGAATTCAATAGGAGCATTGAAAGAACATAGCTATCATAGTCCTAGGATGAGCCC
TGGAGCACTTGGGGCTACTCCTGCAGATAAAGCTGGTGTGATTGAGTTGATCATTCCTTCCGTCTTTAGAGCCGTAGTATCTCTGCATCCAGTTGGTTCTGTTGAACCAG
ATGCTCTCGCTTTCTTTTCTCCTGATGAGAGAGGCAGCCATGTACATGCCAGGGGTTTTTCAAATTATCATTTGTTCAGACATGTTACGGAGCATGCTGCCACAGTGCTG
CAGTATTTTCTTGGAAATCAACCAAAAGCAGCTCTTTATCCTCTATTGCTTTGGATCTGCAGCTACCAAAATTTATTTTCAAAAGCTTGTAGTAAATGTGGACGGCGTTT
ATCAGTAAACAAACAATCCGATTTATTGTTACCTCCGGCAGTTCGGCCGTACAAACAGTTTTGTGCATCAAAAAACTCATCAGTTGTGCCCATAACCTCATTGAAAGACC
AGAAGCTGGATTCTGTTCAAGCTTTCCACATTGATTGTTTCTCAGAAGAAATATAG

mRNA sequence

Show/hide mRNA sequence

TGAAGTATTGTTACTATTATCATTATTATTTTAAAATAAGAGAAAAGAAAAGAAAAGAAAAGGGAGAGGTTGGAAATTGAGAAATTAGCGGTCGTCTTGGTCACACCTCC
GAAGCCTTTTGGGGAAAGAAATTGGAAGCAGTGAACATCGTCGGAGGGGTAAATGCGACATATTCAGCAGCCATCGCAGGGCCAAACTGCGACGGTCAGCCCTAGCACTC
ACTCTCCCCCCTCCCCCGACGACGCTCCTCCCAAGCAGGTTGCTCTCGCCATGGACAGACTCGGCCACGCCGCTCGTCTCATCGCCGACATCAGACTCGGCGCTGATCGC
CTTCTCGAAGCCCTATGCGTCACTGCTCAGCCTCATCAAAGCTCTAAGCCCCTCCATTTGTTTCAGAAAGAAGACGCTTCCATGCGCCAACACCTCCTCGACCTTCGTGC
AGTTGGAAAGCAGCTTGAAGAGTCTGGCGTTCTCAGTGAATCTCTTCTTTCGAGAAGTAACTCTTGGGGTCTACATATGCCTTTAGTGTGTCCTGATGGCGCTGTGGTTG
CTTATGCTTGGAAACGTCAATTGGCTGGCCAGGCTGGTGCATCTGCAGTTGACCGAACAAGGTTAGCTTTAAAGGCCTTCACTGACCAAAAAAGACGGTTTTTCCCTCAT
CTTGAAGATGAAAATGCGTCAAATAATGAGCCTGCTCTGAAGAAGCCTTGTTTTTCTCATGGAGTGACAGATGTCAAACAAAATGAGCTTATTGATTCTAGATCACTCTC
GGATATTTTATCCTGTATGGAAAAGGAAGTGCCAGAGCTTAAGATTTTCACTTATGAGCGATTGGACTGGTTAAAACAAGCTTCGGCCCTTCCCTCTCAAGCAAATGAGA
ATTCAATAGGAGCATTGAAAGAACATAGCTATCATAGTCCTAGGATGAGCCCTGGAGCACTTGGGGCTACTCCTGCAGATAAAGCTGGTGTGATTGAGTTGATCATTCCT
TCCGTCTTTAGAGCCGTAGTATCTCTGCATCCAGTTGGTTCTGTTGAACCAGATGCTCTCGCTTTCTTTTCTCCTGATGAGAGAGGCAGCCATGTACATGCCAGGGGTTT
TTCAAATTATCATTTGTTCAGACATGTTACGGAGCATGCTGCCACAGTGCTGCAGTATTTTCTTGGAAATCAACCAAAAGCAGCTCTTTATCCTCTATTGCTTTGGATCT
GCAGCTACCAAAATTTATTTTCAAAAGCTTGTAGTAAATGTGGACGGCGTTTATCAGTAAACAAACAATCCGATTTATTGTTACCTCCGGCAGTTCGGCCGTACAAACAG
TTTTGTGCATCAAAAAACTCATCAGTTGTGCCCATAACCTCATTGAAAGACCAGAAGCTGGATTCTGTTCAAGCTTTCCACATTGATTGTTTCTCAGAAGAAATATAGTC
ACACTTAGCGGTTCAGGGCCTCATGGACTCTGATTCAAATGATATACGGCAATATCCTCAGAATCAGAGGATGGCCCAGGCTGCTATGCTATCTTGTGCTGCTTTTGGTG
ATTGCAATTACATGCACGGAATGATATTCACATTCAGATTACACATAGGAGCTAATCTAATCTAATCGTGATTTCGAAAATGACCATCTGTTACTGATGCGGTTATTTTT
GTTGTCTCAAGTCGCTTGCCATCATCAAGCCAGGCTAAAGATTCAAGTGAAGGAAGTTAGGGTTTCATTAGTATGAGCGTGCATATGTACAATACTCTAAAGTTTGGATC
AGCAGAAGAGCGAAAAACTTTAGTAAACATTTGAACCTCAAGAGTCAATAGTTATGAAAACATTTT

Protein sequence

Show/hide protein sequence

MRHIQQPSQGQTATVSPSTHSPPSPDDAPPKQVALAMDRLGHAARLIADIRLGADRLLEALCVTAQPHQSSKPLHLFQKEDASMRQHLLDLRAVGKQLEESGVLSESLLS
RSNSWGLHMPLVCPDGAVVAYAWKRQLAGQAGASAVDRTRLALKAFTDQKRRFFPHLEDENASNNEPALKKPCFSHGVTDVKQNELIDSRSLSDILSCMEKEVPELKIFT
YERLDWLKQASALPSQANENSIGALKEHSYHSPRMSPGALGATPADKAGVIELIIPSVFRAVVSLHPVGSVEPDALAFFSPDERGSHVHARGFSNYHLFRHVTEHAATVL
QYFLGNQPKAALYPLLLWICSYQNLFSKACSKCGRRLSVNKQSDLLLPPAVRPYKQFCASKNSSVVPITSLKDQKLDSVQAFHIDCFSEEI

CSPI03G35600 (gene) of Cucumber (PI 183967) v1 genome