CuGenDBv2

Gene ID	CSPI03G43160
Organism	Cucumis sativus L. var. sativus cv. PI 183967 (Cucumber (PI 183967) v1)
Description	LINE-1 retrotransposable element ORF2 protein
Genome location	Chr3:37082577..37082950
RNA-Seq Expression	CSPI03G43160
Synteny	CSPI03G43160
Gene Ontology terms	GO:0009987 - cellular process (biological process) GO:0003824 - catalytic activity (molecular function)
InterPro domains	IPR000477 - Reverse transcriptase domain

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0056839.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]

7.7e-28

57.26

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Query:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD
        ML KKHF  KWRKWI +CIS VQYSIL+NG   G+I   RGIRQGDPLSPFIFV+AMDYLSR+L HLE  G + G                D+L+F+ED+
Subjt:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD

Query:  DENIINLQFAIHLFEKA
        +  + NLQ A+ LFEKA
Subjt:  DENIINLQFAIHLFEKA

KAA0057507.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]

7.7e-28

57.26

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Query:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD
        ML KKHF  KWRKWI +CIS VQYSIL+NG   G+I   RGIRQGDPLSPFIFV+AMDYLSR+L HLE  G + G                D+L+F+ED+
Subjt:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD

Query:  DENIINLQFAIHLFEKA
        +  + NLQ A+ LFEKA
Subjt:  DENIINLQFAIHLFEKA

KAA0058887.1 reverse transcriptase [Cucumis melo var. makuwa]

1.2e-28

58.62

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Query:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG---------------DILLFIEDDD
        MLKKK F  KWR+W  SCI+ VQYSILINGK +GKI P RGIRQGD +SPFIFV+A+DYLS +L+HL+ +  + G               DI+LF++DDD
Subjt:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG---------------DILLFIEDDD

Query:  ENIINLQFAIHLFEKA
        E+I NLQF IHLFE A
Subjt:  ENIINLQFAIHLFEKA

TYK08190.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]

7.7e-28

57.26

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Query:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD
        ML KKHF  KWRKWI +CIS VQYSIL+NG   G+I   RGIRQGDPLSPFIFV+AMDYLSR+L HLE  G + G                D+L+F+ED+
Subjt:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD

Query:  DENIINLQFAIHLFEKA
        +  + NLQ A+ LFEKA
Subjt:  DENIINLQFAIHLFEKA

TYK28859.1 LINE-1 retrotransposable element ORF2 protein [Cucumis melo var. makuwa]

7.7e-28

57.26

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Query:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD
        ML KKHF  KWRKWI +CIS VQYSIL+NG   G+I   RGIRQGDPLSPFIFV+AMDYLSR+L HLE  G + G                D+L+F+ED+
Subjt:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD

Query:  DENIINLQFAIHLFEKA
        +  + NLQ A+ LFEKA
Subjt:  DENIINLQFAIHLFEKA

TrEMBL top hits

e value

%identity

Alignment

A0A5A7US62 LINE-1 retrotransposable element ORF2 protein

3.7e-28

57.26

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Query:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD
        ML KKHF  KWRKWI +CIS VQYSIL+NG   G+I   RGIRQGDPLSPFIFV+AMDYLSR+L HLE  G + G                D+L+F+ED+
Subjt:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD

Query:  DENIINLQFAIHLFEKA
        +  + NLQ A+ LFEKA
Subjt:  DENIINLQFAIHLFEKA

A0A5A7UTI6 LINE-1 retrotransposable element ORF2 protein

3.7e-28

57.26

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Query:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD
        ML KKHF  KWRKWI +CIS VQYSIL+NG   G+I   RGIRQGDPLSPFIFV+AMDYLSR+L HLE  G + G                D+L+F+ED+
Subjt:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD

Query:  DENIINLQFAIHLFEKA
        +  + NLQ A+ LFEKA
Subjt:  DENIINLQFAIHLFEKA

A0A5D3CA17 LINE-1 retrotransposable element ORF2 protein

3.7e-28

57.26

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Query:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD
        ML KKHF  KWRKWI +CIS VQYSIL+NG   G+I   RGIRQGDPLSPFIFV+AMDYLSR+L HLE  G + G                D+L+F+ED+
Subjt:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD

Query:  DENIINLQFAIHLFEKA
        +  + NLQ A+ LFEKA
Subjt:  DENIINLQFAIHLFEKA

A0A5D3DJA1 Reverse transcriptase

5.7e-29

58.62

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Query:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG---------------DILLFIEDDD
        MLKKK F  KWR+W  SCI+ VQYSILINGK +GKI P RGIRQGD +SPFIFV+A+DYLS +L+HL+ +  + G               DI+LF++DDD
Subjt:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG---------------DILLFIEDDD

Query:  ENIINLQFAIHLFEKA
        E+I NLQF IHLFE A
Subjt:  ENIINLQFAIHLFEKA

A0A5D3DZM5 LINE-1 retrotransposable element ORF2 protein

3.7e-28

57.26

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Query:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD
        ML KKHF  KWRKWI +CIS VQYSIL+NG   G+I   RGIRQGDPLSPFIFV+AMDYLSR+L HLE  G + G                D+L+F+ED+
Subjt:  MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSG----------------DILLFIEDD

Query:  DENIINLQFAIHLFEKA
        +  + NLQ A+ LFEKA
Subjt:  DENIINLQFAIHLFEKA

SwissProt top hits

e value

%identity

Alignment

P16423 Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM

4.7e-04

41.51

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Query:  INGKSWG--KILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSGDIL
        +NG  W   + +P RG++QGDPLSP +F + MD L R L    +IG   G+ +
Subjt:  INGKSWG--KILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSGDIL

P92555 Uncharacterized mitochondrial protein AtMg01250

1.0e-06

36.76

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Query:  LINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSGDILLFIEDDDENIINLQFA
        +ING   G + P RG+RQGDPLSP++F++  + LS + +  ++ G L G   + + ++   I +L FA
Subjt:  LINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSGDILLFIEDDDENIINLQFA

Q05118 Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment)

2.1e-04

33.33

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Query:  DKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIG-----------MLSGDILLFIEDDDENIIN
        D  + +I S I+    +I++ G++  KI    G++QGDPLSP +F I +D L   L   EQ G           +   D LL +ED D ++ N
Subjt:  DKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIG-----------MLSGDILLFIEDDDENIIN

Arabidopsis top hits

e value

%identity

Alignment

ATMG01250.1 RNA-directed DNA polymerase (reverse transcriptase)

7.2e-08

36.76

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Query:  LINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSGDILLFIEDDDENIINLQFA
        +ING   G + P RG+RQGDPLSP++F++  + LS + +  ++ G L G   + + ++   I +L FA
Subjt:  LINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSGDILLFIEDDDENIINLQFA

Sequences

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CDS sequence

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ATGTTGAAGAAAAAACATTTTTCAGACAAATGGAGGAAATGGATACATTCTTGTATTTCAACGGTGCAATATTCTATATTGATCAATGGTAAATCATGGGGAAAGATCTT
ACCAGGCAGAGGCATCAGACAAGGAGATCCTCTTTCTCCTTTTATATTTGTGATAGCTATGGATTATCTAAGCAGAATTCTTCAACATCTTGAACAAATTGGCATGCTAT
CAGGTGATATCCTACTTTTCATTGAGGATGATGATGAAAACATTATAAATCTACAATTTGCTATACATCTCTTTGAAAAGGCCTTTGTCAATGGTAGGCAGATCACCGAT

mRNA sequence

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Protein sequence

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MLKKKHFSDKWRKWIHSCISTVQYSILINGKSWGKILPGRGIRQGDPLSPFIFVIAMDYLSRILQHLEQIGMLSGDILLFIEDDDENIINLQFAIHLFEKAFVNGRQITD

CSPI03G43160 (gene) of Cucumber (PI 183967) v1 genome