CuGenDBv2

Gene ID	CSPI04G00530
Organism	Cucumis sativus L. var. sativus cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Mediator of RNA polymerase II transcription subunit 28
Genome location	Chr4:267129..271296
RNA-Seq Expression	CSPI04G00530
Synteny	CSPI04G00530
Gene Ontology terms	GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0016592 - mediator complex (cellular component)
InterPro domains	IPR034456 - Mediator of RNA polymerase II transcription subunit 28

Homology

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GenBank top hits

e value

%identity

Alignment

XP_004152267.1 mediator of RNA polymerase II transcription subunit 28 [Cucumis sativus]

1.2e-68

100

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Query:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
        MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
Subjt:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS

Query:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
        AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
Subjt:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

XP_008454378.1 PREDICTED: mediator of RNA polymerase II transcription subunit 28 [Cucumis melo]

1.2e-65

95.74

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Query:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
        MAERQ +DQQHSLDPQ QSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
Subjt:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS

Query:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
        AIEEELKVKN+IIKKQE+LIEGWKKDLK+QLDKHNNELEKV
Subjt:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

XP_022149222.1 mediator of RNA polymerase II transcription subunit 28 [Momordica charantia]

1.5e-63

92.2

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Query:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
        MA+RQP+DQQH LDPQLQS QSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYFIGLQREDQP KVETLRKEIS
Subjt:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS

Query:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
        AIEEELK+KN+IIKK E+LIEGWKKDLKDQL KHNNELEKV
Subjt:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

XP_022922953.1 mediator of RNA polymerase II transcription subunit 28 [Cucurbita moschata]

1.0e-64

92.2

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Query:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
        MAERQP+DQQ+ +DPQLQSSQSPREDM++YVMALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
Subjt:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS

Query:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
        AIEEELK+K++IIKKQE+LIEGWKKDLKDQLDKHNNELEKV
Subjt:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

XP_038904970.1 mediator of RNA polymerase II transcription subunit 28 [Benincasa hispida]

1.4e-66

96.45

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Query:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
        MAERQP+DQQH LDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQP KVETLRKEIS
Subjt:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS

Query:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
        AIEEELK+KNEIIKKQE+LIEGWKKDLKDQLDKHNNELEKV
Subjt:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KT19 Uncharacterized protein

5.6e-69

100

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Query:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
        MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
Subjt:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS

Query:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
        AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
Subjt:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

A0A1S3BZ89 mediator of RNA polymerase II transcription subunit 28

5.8e-66

95.74

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Query:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
        MAERQ +DQQHSLDPQ QSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
Subjt:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS

Query:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
        AIEEELKVKN+IIKKQE+LIEGWKKDLK+QLDKHNNELEKV
Subjt:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

A0A5A7TRZ8 Mediator of RNA polymerase II transcription subunit 28

5.8e-66

95.74

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Query:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
        MAERQ +DQQHSLDPQ QSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
Subjt:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS

Query:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
        AIEEELKVKN+IIKKQE+LIEGWKKDLK+QLDKHNNELEKV
Subjt:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

A0A6J1E897 mediator of RNA polymerase II transcription subunit 28

4.9e-65

92.2

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Query:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
        MAERQP+DQQ+ +DPQLQSSQSPREDM++YVMALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
Subjt:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS

Query:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
        AIEEELK+K++IIKKQE+LIEGWKKDLKDQLDKHNNELEKV
Subjt:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

A0A6J1JAT8 mediator of RNA polymerase II transcription subunit 28

4.9e-65

92.2

Show/hide

Query:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
        MAERQP+DQQ+ +DPQLQSSQSPREDM++YVMALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS
Subjt:  MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEIS

Query:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
        AIEEELK+K++IIKKQE+LIEGWKKDLKDQLDKHNNELEKV
Subjt:  AIEEELKVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

SwissProt top hits

e value

%identity

Alignment

Q9LFA5 Mediator of RNA polymerase II transcription subunit 28

2.9e-38

62.96

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Query:  PQLQSSQSPR-------EDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQ-PTKVETLRKEISAIEEEL
        P ++S ++P        ED++A V ALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYF+GL+RED+ P++ E+L+K+I+ +EEEL
Subjt:  PQLQSSQSPR-------EDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQ-PTKVETLRKEISAIEEEL

Query:  KVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
        K K+E+IKK  +L +  +K +K+Q++KH +ELEKV
Subjt:  KVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

Arabidopsis top hits

e value

%identity

Alignment

AT3G52860.1 unknown protein

2.0e-39

62.96

Show/hide

Query:  PQLQSSQSPR-------EDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQ-PTKVETLRKEISAIEEEL
        P ++S ++P        ED++A V ALEAALLPCLPARELQAIDRSPHPSHQ+DVERHARDFMEAAKKLQLYF+GL+RED+ P++ E+L+K+I+ +EEEL
Subjt:  PQLQSSQSPR-------EDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQ-PTKVETLRKEISAIEEEL

Query:  KVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV
        K K+E+IKK  +L +  +K +K+Q++KH +ELEKV
Subjt:  KVKNEIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

Sequences

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CDS sequence

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ATGGCTGAACGACAACCAATCGACCAACAACATTCGCTTGATCCACAATTGCAATCATCACAGTCTCCAAGAGAAGATATGATTGCATATGTGATGGCCTTGGAAGCTGC
TCTGCTTCCGTGCTTGCCCGCAAGAGAACTACAGGCAATAGACCGTTCTCCTCACCCTTCTCATCAAGTTGACGTGGAGAGACATGCTAGGGATTTCATGGAAGCAGCTA
AAAAGCTTCAATTGTATTTCATTGGTTTACAGCGTGAGGATCAACCAACCAAAGTGGAAACACTAAGAAAGGAGATATCTGCCATTGAGGAGGAATTAAAGGTAAAAAAT
GAAATAATCAAGAAACAGGAGAAACTCATTGAAGGATGGAAGAAGGATTTGAAAGACCAGTTAGACAAACATAACAATGAGTTAGAGAAAGTTTAA

mRNA sequence

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GGAAAAAAGGAGCGGAGAAGTGAAGGAAGGCGTCGGTAGTAGACGCTCCGTCTCCTTCTCTTCCTTTCCTTTGCGTCCGCCGTCCGATCAACAGTTCCTCGAGAAGCAAA
CCGACGACCTCTTGCTCCACCTTTGACGGTGGAGCTACCTTGCGACCTGAACACTCAGTTCGAACAAGTTGCATATCATCGTTTCTTCTCCATTTCACACTACAAGTTCT
GCATCACGTTTTTTCACGACTGGGTGTGTCTTGCAGCAACGTTTTTGGCTTGTGGGATATGGCTGAACGACAACCAATCGACCAACAACATTCGCTTGATCCACAATTGC
AATCATCACAGTCTCCAAGAGAAGATATGATTGCATATGTGATGGCCTTGGAAGCTGCTCTGCTTCCGTGCTTGCCCGCAAGAGAACTACAGGCAATAGACCGTTCTCCT
CACCCTTCTCATCAAGTTGACGTGGAGAGACATGCTAGGGATTTCATGGAAGCAGCTAAAAAGCTTCAATTGTATTTCATTGGTTTACAGCGTGAGGATCAACCAACCAA
AGTGGAAACACTAAGAAAGGAGATATCTGCCATTGAGGAGGAATTAAAGGTAAAAAATGAAATAATCAAGAAACAGGAGAAACTCATTGAAGGATGGAAGAAGGATTTGA
AAGACCAGTTAGACAAACATAACAATGAGTTAGAGAAAGTTTAATTAGAATAGGTTTGTGATCCCATGCTCTATTGTTTAGTTGTTTGTAGATCTTGTTAATTACGTACT
TTCACTGTGAATGTAGGTGGGCTTATTGACTAGTCTTGCTTAACTACGTTCATTGTGCGGTTGTGCCTAGTTTTCTTCTTAAATGTTCGAAATAGTAATGAATTTTATTT
AACAAAATTTGACTTAAAAATTGTTTATTGAACCTACGAACTCTTCATCTTGG

Protein sequence

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MAERQPIDQQHSLDPQLQSSQSPREDMIAYVMALEAALLPCLPARELQAIDRSPHPSHQVDVERHARDFMEAAKKLQLYFIGLQREDQPTKVETLRKEISAIEEELKVKN
EIIKKQEKLIEGWKKDLKDQLDKHNNELEKV

CSPI04G00530 (gene) of Cucumber (PI 183967) v1 genome