CuGenDBv2

Gene ID	CSPI04G16230
Organism	Cucumis sativus L. var. sativus cv. PI 183967 (Cucumber (PI 183967) v1)
Description	macrophage migration inhibitory factor homolog
Genome location	Chr4:13516687..13519649
RNA-Seq Expression	CSPI04G16230
Synteny	CSPI04G16230
Gene Ontology terms	GO:0005615 - extracellular space (cellular component) GO:0050178 - phenylpyruvate tautomerase activity (molecular function)
InterPro domains	IPR001398 - Macrophage migration inhibitory factor IPR014347 - Tautomerase/MIF superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6595575.1 hypothetical protein SDJN03_12128, partial [Cucurbita argyrosperma subsp. sororia]

8.6e-54

95.65

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCLN+STNVNLEGIDTS+VLSEASSTVAKIIGKPEAYVM+VLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAA+SAILETKLSVPKSRFFLKF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
        YDTKGSN GWNGSTF
Subjt:  YDTKGSNLGWNGSTF

XP_011653708.1 macrophage migration inhibitory factor homolog isoform X2 [Cucumis sativus]

4.5e-55

100

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
        YDTKGSNLGWNGSTF
Subjt:  YDTKGSNLGWNGSTF

XP_022925084.1 macrophage migration inhibitory factor homolog isoform X2 [Cucurbita moschata]

3.8e-54

96.52

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCLN+STNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVM+VLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAA+SAILETKLSVPKSRFFLKF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
        YDTKGSN GWNGSTF
Subjt:  YDTKGSNLGWNGSTF

XP_022966490.1 macrophage migration inhibitory factor homolog isoform X2 [Cucurbita maxima]

2.9e-54

96.52

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCLN+STNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVM+VLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAA+SAILETKLSVPKSRFFLKF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
        YDTKGSN GWNGSTF
Subjt:  YDTKGSNLGWNGSTF

XP_038881028.1 macrophage migration inhibitory factor homolog [Benincasa hispida]

2.9e-54

97.39

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCLNISTNVNLEG+DTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGG+EQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
        YDTKGSNLGWNG+TF
Subjt:  YDTKGSNLGWNGSTF

TrEMBL top hits

e value

%identity

Alignment

A0A0A0L2R6 Uncharacterized protein

2.2e-55

100

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
        YDTKGSNLGWNGSTF
Subjt:  YDTKGSNLGWNGSTF

A0A1S3TFC2 macrophage migration inhibitory factor homolog isoform X2

3.9e-52

91.3

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCLN+STNVNL+G+DTSS+LSEA+STVA IIGKPEAYVMIVLKGSVP+SFGG+EQPAAYGELVSIGGLNPDVNKKLSAAI+AILETKLSVPKSR+FLKF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
        YDTKGSNLGWNGSTF
Subjt:  YDTKGSNLGWNGSTF

A0A6J1CSE6 macrophage migration inhibitory factor homolog

1.0e-52

93.04

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCLN+STNVNL+G+DTSSVLSEASSTVA+IIGKPEAYVM+VLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAA+S ILETKLSVPKSRFFLKF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
        YDTKGSN GWNGSTF
Subjt:  YDTKGSNLGWNGSTF

A0A6J1EB33 macrophage migration inhibitory factor homolog isoform X2

1.9e-54

96.52

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCLN+STNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVM+VLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAA+SAILETKLSVPKSRFFLKF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
        YDTKGSN GWNGSTF
Subjt:  YDTKGSNLGWNGSTF

A0A6J1HRS5 macrophage migration inhibitory factor homolog isoform X2

1.4e-54

96.52

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCLN+STNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVM+VLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAA+SAILETKLSVPKSRFFLKF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
        YDTKGSN GWNGSTF
Subjt:  YDTKGSNLGWNGSTF

SwissProt top hits

e value

%identity

Alignment

O44786 Macrophage migration inhibitory factor homolog

2.1e-15

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MP   I TN   + I +S+ L +A + V K +GKPE+YV I + G  PM FGGSE P     L SIG + P VN   +  +  +L  +L +PK+R +++ 
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
         D + S++ +NGSTF
Subjt:  YDTKGSNLGWNGSTF

P81529 Macrophage migration inhibitory factor homolog

1.6e-18

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MP   ++TN+    +  S  LS  S+ V  I+ KP +YV + +     +SFGGS +PAA+G L+SIGG+ P  N+  SA +   L  KL +PK+R ++ F
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
         +  G ++GWNG+TF
Subjt:  YDTKGSNLGWNGSTF

P81748 Macrophage migration inhibitory factor homolog

6.0e-18

39.13

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MP    STNV  E I     L   S  +A ++GKPE+YV + + G   ++FGG++ PA +G+L+S+GG+  + N+  SA +   L   L +P +R ++ F
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
         D +GS++G+NGSTF
Subjt:  YDTKGSNLGWNGSTF

P91850 Macrophage migration inhibitory factor homolog

1.8e-17

42.61

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MP   I TN+    I +S+ L +AS+ VAK +GKPE+YV I + G   M FGGSE P A   L SIG + P VN   +  +  +L  +L +PK+R +++F
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
         D + S++ +NGSTF
Subjt:  YDTKGSNLGWNGSTF

Q76BK2 Macrophage migration inhibitory factor

1.8e-14

37.39

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MP   I TNV  + +   ++LS+ +  +AK  GKP  Y+ I +     MSFG S  P A   L SIG +    NK  +  +  IL  +L++P +R ++ +
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
        YD   +N+GWNGSTF
Subjt:  YDTKGSNLGWNGSTF

Arabidopsis top hits

e value

%identity

Alignment

AT3G51660.1 Tautomerase/MIF superfamily protein

1.9e-22

47.06

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCL I+TNVN +G++T    SE +  VA I+G+P+  VM+VLKGSV + FGG+++ AAY E+VS+GG+   V ++L A + +IL T  S+  +RF  K 
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YD
        +D
Subjt:  YD

AT5G01650.1 Tautomerase/MIF superfamily protein

4.5e-53

89.47

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCLN+STNVNL+G+DTSS+LSEASSTVAKIIGKPE YVMIVLKGSVPMSFGG+E PAAYGELVSIGGLN DVNKKLSAA+SAILETKLSVPKSRFFLKF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGST
        YDTKGS  GWNG+T
Subjt:  YDTKGSNLGWNGST

AT5G01650.2 Tautomerase/MIF superfamily protein

4.4e-48

91.35

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MPCLN+STNVNL+G+DTSS+LSEASSTVAKIIGKPE YVMIVLKGSVPMSFGG+E PAAYGELVSIGGLN DVNKKLSAA+SAILETKLSVPKSRFFLKF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTK
        YDTK
Subjt:  YDTK

AT5G57170.1 Tautomerase/MIF superfamily protein

5.2e-33

57.39

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MP LN+ TN+ ++ +  S +L +A+  VAKIIGKPE+YVMI+L   VP++F G+E+PAAYGEL+SIGGL P VN KLS  IS IL+ KLS+  SRF++KF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNLGWNGSTF
        YD+     G+NGSTF
Subjt:  YDTKGSNLGWNGSTF

AT5G57170.2 Tautomerase/MIF superfamily protein

5.4e-30

57.41

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Query:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF
        MP LN+ TN+ ++ +  S +L +A+  VAKIIGKPE+YVMI+L   VP++F G+E+PAAYGEL+SIGGL P VN KLS  IS IL+ KLS+  SRF++KF
Subjt:  MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKF

Query:  YDTKGSNL
        YD+  S L
Subjt:  YDTKGSNL

Sequences

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CDS sequence

Show/hide CDS sequence

ATGCCTTGCCTTAACATTTCTACAAATGTCAACCTTGAAGGTATCGACACCTCCTCTGTTCTTTCTGAAGCTTCATCTACCGTTGCCAAGATCATTGGCAAGCCAGAAGC
CTACGTAATGATTGTGCTGAAGGGATCGGTACCGATGTCATTTGGGGGCAGTGAGCAGCCAGCAGCATATGGTGAGTTGGTGTCCATCGGTGGTCTGAACCCTGATGTCA
ACAAGAAATTGAGTGCTGCAATTTCTGCAATCCTGGAGACCAAGCTATCTGTTCCCAAGTCAAGATTCTTTCTCAAATTCTACGACACCAAGGGTTCCAACTTGGGATGG
AATGGCTCCACTTTCTAA

mRNA sequence

Show/hide mRNA sequence

GAAAACGAGGGCAGACGATTCTTCAGATAAGAATTCACTGAACACACAAGGAGGAGAAGAAGAAGAAGAAGAAGAAGAAGATGCCTTGCCTTAACATTTCTACAAATGTC
AACCTTGAAGGTATCGACACCTCCTCTGTTCTTTCTGAAGCTTCATCTACCGTTGCCAAGATCATTGGCAAGCCAGAAGCCTACGTAATGATTGTGCTGAAGGGATCGGT
ACCGATGTCATTTGGGGGCAGTGAGCAGCCAGCAGCATATGGTGAGTTGGTGTCCATCGGTGGTCTGAACCCTGATGTCAACAAGAAATTGAGTGCTGCAATTTCTGCAA
TCCTGGAGACCAAGCTATCTGTTCCCAAGTCAAGATTCTTTCTCAAATTCTACGACACCAAGGGTTCCAACTTGGGATGGAATGGCTCCACTTTCTAAGTTAACTTCATC
TGTAAGCTCTTGAAATTCCCAACTTTTATATTTGCTGGGACCTAGTTATAAGGATTTGGTATTGTTAACCCTTCATATTATATTGAGTTCTGTCTAGATTCCCTGTGACT
TTTGGTTATGAATCATAGATCGAGTTATAATCTACCAAGTTTTAATGAGTACTTTTATTGGTTTAACACTCTTAACATTTTTAATCATTTTGAAAGACATACCTTAATAC
CTTAAGAAGTTTGCAGAATATACCTTTAATTGTTTTTTATTTTT

Protein sequence

Show/hide protein sequence

MPCLNISTNVNLEGIDTSSVLSEASSTVAKIIGKPEAYVMIVLKGSVPMSFGGSEQPAAYGELVSIGGLNPDVNKKLSAAISAILETKLSVPKSRFFLKFYDTKGSNLGW
NGSTF

CSPI04G16230 (gene) of Cucumber (PI 183967) v1 genome