CuGenDBv2

Gene ID	Carg00396
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial
Genome location	Carg_Chr04:7012595..7015521
RNA-Seq Expression	Carg00396
Synteny	Carg00396
Gene Ontology terms	GO:0006450 - regulation of translational fidelity (biological process) GO:0032543 - mitochondrial translation (biological process) GO:0070681 - glutaminyl-tRNAGln biosynthesis via transamidation (biological process) GO:0005739 - mitochondrion (cellular component) GO:0009507 - chloroplast (cellular component) GO:0030956 - glutamyl-tRNA(Gln) amidotransferase complex (cellular component) GO:0005524 - ATP binding (molecular function) GO:0016740 - transferase activity (molecular function) GO:0050567 - glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity (molecular function)
InterPro domains	IPR003837 - Glu-tRNAGln amidotransferase C subunit IPR036113 - Glu-tRNAGln amidotransferase superfamily, subunit C

Homology

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GenBank top hits

e value

%identity

Alignment

KAG7031739.1 Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma]

8.6e-81

100

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Query:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
        MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
Subjt:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS

Query:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
Subjt:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

XP_022941306.1 glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial [Cucurbita moschata]

2.9e-60

83.65

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Query:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
        MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
Subjt:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS

Query:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        IRAD E      D  E  +        NR      EAMIGAVPVFDEPYIRVPKVLNKE
Subjt:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

XP_022981070.1 glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial-like [Cucurbita maxima]

3.9e-57

79.25

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Query:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
        MGSGALRLLKGASSLP LNPQIFISTSLN+LPVS+IRI+KCSTAPGIRASLQPPDVPRLAETARISLNP+EVEEFAPKIRQVVDWFGQL+AVDLQSIEPS
Subjt:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS

Query:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        IRAD E      D  E  +        NR      EA+IGAVPVFDEPYIRVPKVLNKE
Subjt:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

XP_023521629.1 glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo]

1.2e-58

81.76

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Query:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
        M SGALRLLKGASSLPSLNPQIFISTSLNSLPVS+IRIRKCSTAPGIRASLQPPDVPRLAETARISLNP+EVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
Subjt:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS

Query:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        IRAD E      D  E  +        NR      EAMIGAVPVFDEPYIRVPKVLNKE
Subjt:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

XP_038897282.1 glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial [Benincasa hispida]

9.6e-48

71.7

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Query:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
        MGS AL LLKGASS PSL  +IFIST L+SLP S IRIRKCSTA  I ASLQPPDVPRLAETARISL P +VEEFAPKIRQVVDWFGQLQ VDLQSIEP+
Subjt:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS

Query:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        IRAD E     +D  E  +        NR      EAMIGAVPVFDEPYI+VPKVLNKE
Subjt:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

TrEMBL top hits

e value

%identity

Alignment

A0A1S3CQE8 Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial

1.4e-47

70.44

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Query:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
        MGS AL LLKGASS PSL  QIFIST+ +SLPVS+I IRK STA G+ ASLQPPDVPRLA+TARISL P +VEEFAPKI QVVDWFGQLQ VDLQSIEPS
Subjt:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS

Query:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        IRAD E     +D  E  +        NR      EAMIGAVPVFDEPYI+VPKVLNKE
Subjt:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

A0A5A7T6R5 Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial

1.4e-47

70.44

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Query:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
        MGS AL LLKGASS PSL  QIFIST+ +SLPVS+I IRK STA G+ ASLQPPDVPRLA+TARISL P +VEEFAPKI QVVDWFGQLQ VDLQSIEPS
Subjt:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS

Query:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        IRAD E     +D  E  +        NR      EAMIGAVPVFDEPYI+VPKVLNKE
Subjt:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

A0A6J1CQ90 Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial

2.3e-47

70.81

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Query:  MGSGALRLLKGASSLPSLNPQIFI--STSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIE
        MGS AL LLKGA SLPSL  QIFI      ++LP+SS +IRKCST P IRASLQPPDVPRLAETARISL P EVEEFAPKIRQVVDWFGQLQ VDLQSIE
Subjt:  MGSGALRLLKGASSLPSLNPQIFI--STSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIE

Query:  PSIRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        PSIRAD E     +D  E  +        NR      EAMIGAVPVFDEPYIRVPKVLNKE
Subjt:  PSIRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

A0A6J1FM33 Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial

1.4e-60

83.65

Show/hide

Query:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
        MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
Subjt:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS

Query:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        IRAD E      D  E  +        NR      EAMIGAVPVFDEPYIRVPKVLNKE
Subjt:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

A0A6J1IVG1 Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial

1.9e-57

79.25

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Query:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
        MGSGALRLLKGASSLP LNPQIFISTSLN+LPVS+IRI+KCSTAPGIRASLQPPDVPRLAETARISLNP+EVEEFAPKIRQVVDWFGQL+AVDLQSIEPS
Subjt:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS

Query:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        IRAD E      D  E  +        NR      EA+IGAVPVFDEPYIRVPKVLNKE
Subjt:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

SwissProt top hits

e value

%identity

Alignment

A2XK57 Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial

2.8e-26

56.36

Show/hide

Query:  SLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPSIRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPY
        +L+PPD+PRLA  ARISL+P E EEFAPKIRQVVDWFGQLQAVDL+S+EPS+RA   A       G +++       +NR      +A+I +VP +D+PY
Subjt:  SLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPSIRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPY

Query:  IRVPKVLNKE
        I+VP+VLNKE
Subjt:  IRVPKVLNKE

B9INH0 Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial

4.0e-28

50.31

Show/hide

Query:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
        MGS A+ LLKG S  P  +  +F+       P  SIR R  + A    +SL+PPDV RLAETARISL P +VEEF PKIRQV+DW   L +VDL S+EPS
Subjt:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS

Query:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        IRAD E     HD  E  +        NR      EA+I AVP +++PY++VPKVLNKE
Subjt:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

B9RRX2 Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial

3.8e-31

55.62

Show/hide

Query:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRI-RKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEP
        MGS AL LLK A  LP L  QI         P S +   R+ S      +SLQPPDV RLAETARISLN NEVEEFAPKIRQV+DWFGQLQAVDL S+EP
Subjt:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRI-RKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEP

Query:  SIRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        +IRAD E        GE ++  V    S R      EA+I AVP +++PY++VPKVLNKE
Subjt:  SIRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

D7STK2 Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial

4.5e-32

52.2

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Query:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS
        MGS AL LLK  ++      +  + TS ++   S   IR  + +   R++L PPD+PRLAETARISL P+EVEEFAPKIRQV+DWFGQLQAVDLQSIEPS
Subjt:  MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPS

Query:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE
        IRAD E      D+ E  +        NR      EA+I A+P ++EPY++VPKVLNK+
Subjt:  IRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

Q7XZZ1 Glutamyl-tRNA(Gln) amidotransferase subunit C, chloroplastic/mitochondrial

2.8e-26

56.36

Show/hide

Query:  SLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPSIRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPY
        +L+PPD+PRLA  ARISL+P E EEFAPKIRQVVDWFGQLQAVDL+S+EPS+RA   A       G +++       +NR      +A+I +VP +D+PY
Subjt:  SLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPSIRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPY

Query:  IRVPKVLNKE
        I+VP+VLNKE
Subjt:  IRVPKVLNKE

Arabidopsis top hits

e value

%identity

Alignment

AT4G32915.1 FUNCTIONS IN: molecular_function unknown; INVOLVED IN: regulation of translational fidelity; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glu-tRNAGln amidotransferase, C subunit (InterPro:IPR003837); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

4.3e-22

43.9

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Query:  RIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPSIRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLE
        RI +  ++    + LQPPDV RLA+TARISL P E+EE   KIR+V+DWFGQLQ VD+ S+EP+IRA+++      D  E           NR      +
Subjt:  RIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPSIRADVEALPTSHDTGEAMKHIVVGDSSNRTRNLPLE

Query:  AMIGAVPVFDEPYIRVPKVLNKE
        ++  ++P F++ Y++VPK+LNKE
Subjt:  AMIGAVPVFDEPYIRVPKVLNKE

Sequences

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CDS sequence

Show/hide CDS sequence

ATGGGAAGTGGAGCTCTGCGTTTGCTCAAAGGAGCTTCATCACTTCCATCGTTGAATCCCCAAATCTTCATCTCTACCAGTTTGAATTCGCTTCCTGTTTCTAGTATCAG
AATCCGGAAATGTTCGACTGCTCCTGGAATTCGTGCCTCTCTTCAACCTCCCGATGTTCCTCGTTTAGCTGAAACAGCTCGAATTTCCCTCAATCCGAATGAGGTTGAAG
AATTTGCTCCTAAGATTCGACAGGTTGTTGACTGGTTTGGGCAGCTCCAGGCGGTTGATCTTCAAAGCATTGAGCCTTCCATTAGGGCAGATGTGGAGGCATTGCCTACT
TCTCACGACACAGGTGAGGCAATGAAGCACATCGTGGTGGGGGATAGCAGCAATAGGACCCGAAACTTACCACTAGAGGCCATGATTGGCGCAGTTCCAGTCTTTGATGA
GCCGTATATCAGAGTTCCTAAAGTTCTGAATAAGGAGTAG

mRNA sequence

Show/hide mRNA sequence

TGGCGGCTGGTTCCAAATTATATCTTCTTCCCCTTCGCGTTTGGAAATGGGAAGTGGAGCTCTGCGTTTGCTCAAAGGAGCTTCATCACTTCCATCGTTGAATCCCCAAA
TCTTCATCTCTACCAGTTTGAATTCGCTTCCTGTTTCTAGTATCAGAATCCGGAAATGTTCGACTGCTCCTGGAATTCGTGCCTCTCTTCAACCTCCCGATGTTCCTCGT
TTAGCTGAAACAGCTCGAATTTCCCTCAATCCGAATGAGGTTGAAGAATTTGCTCCTAAGATTCGACAGGTTGTTGACTGGTTTGGGCAGCTCCAGGCGGTTGATCTTCA
AAGCATTGAGCCTTCCATTAGGGCAGATGTGGAGGCATTGCCTACTTCTCACGACACAGGTGAGGCAATGAAGCACATCGTGGTGGGGGATAGCAGCAATAGGACCCGAA
ACTTACCACTAGAGGCCATGATTGGCGCAGTTCCAGTCTTTGATGAGCCGTATATCAGAGTTCCTAAAGTTCTGAATAAGGAGTAGTTGCTAAGGTAGGCAGCTTGTTTC
ATCTGTTTCTGTCCTTCATTAATAGCGTGTTCTTCGACATCTTATGTCGCCGAAATGATTAACTTGGCTACAAGTTACTCGGCAATATTCGAATTACAGTTCAAATTAAT
CATCCATAAATCCTAATCTT

Protein sequence

Show/hide protein sequence

MGSGALRLLKGASSLPSLNPQIFISTSLNSLPVSSIRIRKCSTAPGIRASLQPPDVPRLAETARISLNPNEVEEFAPKIRQVVDWFGQLQAVDLQSIEPSIRADVEALPT
SHDTGEAMKHIVVGDSSNRTRNLPLEAMIGAVPVFDEPYIRVPKVLNKE

Carg00396 (gene) of Silver-seed gourd (SMH-JMG-627) v2 genome