CuGenDBv2

Gene ID	Carg02209
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	tRNA (guanine(26)-N(2))-dimethyltransferase
Genome location	Carg_Chr15:1279444..1280985
RNA-Seq Expression	Carg02209
Synteny	Carg02209
Gene Ontology terms	GO:0002940 - tRNA N2-guanine methylation (biological process) GO:0005634 - nucleus (cellular component) GO:0003723 - RNA binding (molecular function) GO:0004809 - tRNA (guanine-N2-)-methyltransferase activity (molecular function)
InterPro domains	IPR002905 - tRNA methyltransferase, Trm1

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6578537.1 putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia]

4.8e-41

96.7

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Query:  VKNHPVKAKPEDQPGIIILAKVLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDESAPNQ
        VKNHPVKAKPEDQPGIIILAKVLKAQARK ARFLPNPERHWGSKLR GRQITSVYISLLDAEAIIGALSHTDYE+PKAQRPKVEDESAPNQ
Subjt:  VKNHPVKAKPEDQPGIIILAKVLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDESAPNQ

KAG7016097.1 putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyrosperma subsp. argyrosperma]

4.4e-55

100

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Query:  MQRMEMKIEEGLIRELCLEASEVKNHPVKAKPEDQPGIIILAKVLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKA
        MQRMEMKIEEGLIRELCLEASEVKNHPVKAKPEDQPGIIILAKVLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKA
Subjt:  MQRMEMKIEEGLIRELCLEASEVKNHPVKAKPEDQPGIIILAKVLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKA

Query:  QRPKVEDESAPNQ
        QRPKVEDESAPNQ
Subjt:  QRPKVEDESAPNQ

XP_022939541.1 probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 isoform X1 [Cucurbita moschata]

4.4e-39

94.51

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Query:  KNHPVKAKPEDQPGIIILAKVL-KAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDESAPNQ
        KNHPVKA+PEDQPGIIILAKVL +AQARK ARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYE+PKAQRPKVEDESAPNQ
Subjt:  KNHPVKAKPEDQPGIIILAKVL-KAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDESAPNQ

XP_022939542.1 probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 isoform X2 [Cucurbita moschata]

4.3e-34

93.9

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Query:  KNHPVKAKPEDQPGIIILAKVL-KAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPK
        KNHPVKA+PEDQPGIIILAKVL +AQARK ARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYE+PKAQRPK
Subjt:  KNHPVKAKPEDQPGIIILAKVL-KAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPK

XP_038890035.1 probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida]

1.1e-29

69.52

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Query:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE
        VKNHPVKA+PEDQPG IILAK              + KAQA+K ARFLPNPERHWG KLRAGRQITS +ISLL AEA+ GALSHTD E+P A+RPKVE+E
Subjt:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE

Query:  SAPNQ
         APNQ
Subjt:  SAPNQ

TrEMBL top hits

e value

%identity

Alignment

A0A1S3CA23 tRNA (guanine(26)-N(2))-dimethyltransferase

5.3e-30

69.52

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Query:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE
        VKNHPVKA+PEDQPG IILAK              + KAQA+K ARFLPNPERHWG KLRAGRQITS +ISLL AEA+ GALSHTD E+P A+RPKVE+E
Subjt:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE

Query:  SAPNQ
         APNQ
Subjt:  SAPNQ

A0A5A7T7H3 tRNA (guanine(26)-N(2))-dimethyltransferase

5.3e-30

69.52

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Query:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE
        VKNHPVKA+PEDQPG IILAK              + KAQA+K ARFLPNPERHWG KLRAGRQITS +ISLL AEA+ GALSHTD E+P A+RPKVE+E
Subjt:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE

Query:  SAPNQ
         APNQ
Subjt:  SAPNQ

A0A6J1FG75 probable tRNA (Guanine(26)-N(2))-dimethyltransferase 2 isoform X1

2.2e-39

94.51

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Query:  KNHPVKAKPEDQPGIIILAKVL-KAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDESAPNQ
        KNHPVKA+PEDQPGIIILAKVL +AQARK ARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYE+PKAQRPKVEDESAPNQ
Subjt:  KNHPVKAKPEDQPGIIILAKVL-KAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDESAPNQ

A0A6J1FH33 probable tRNA (Guanine(26)-N(2))-dimethyltransferase 2 isoform X2

2.1e-34

93.9

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Query:  KNHPVKAKPEDQPGIIILAKVL-KAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPK
        KNHPVKA+PEDQPGIIILAKVL +AQARK ARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYE+PKAQRPK
Subjt:  KNHPVKAKPEDQPGIIILAKVL-KAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPK

A0A6J1JNF2 tRNA (guanine(26)-N(2))-dimethyltransferase

5.0e-28

66.67

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Query:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE
        VKNHPVKA+PEDQPG IILAK              + KAQA+K ARFLPNPERHWG KLRAGRQITS +ISLL AEA+ GALSH D E+P A+R KVE++
Subjt:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE

Query:  SAPNQ
         APNQ
Subjt:  SAPNQ

SwissProt top hits

e value

%identity

Alignment

Q9LFU5 Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1

1.9e-16

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Query:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE
        VKNHP+K +  + PG +IL+K              + KAQA+K ARFLPNPE+HWG K+RAGR ITS ++SLL  EA+ G L++   E    +  + E+E
Subjt:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE

Q9SRU7 Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2

4.5e-18

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Query:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE
        VKNHP+KA+  +QPG +IL+K              + KAQA+K ARFLPNPE+HWG KLRAGRQITS ++SL+  EA+ G LS   +E+ K +  + E E
Subjt:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE

Arabidopsis top hits

e value

%identity

Alignment

AT3G02320.1 N2,N2-dimethylguanosine tRNA methyltransferase

3.2e-19

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Query:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE
        VKNHP+KA+  +QPG +IL+K              + KAQA+K ARFLPNPE+HWG KLRAGRQITS ++SL+  EA+ G LS   +E+ K +  + E E
Subjt:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE

AT5G15810.1 N2,N2-dimethylguanosine tRNA methyltransferase

1.3e-17

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Query:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE
        VKNHP+K +  + PG +IL+K              + KAQA+K ARFLPNPE+HWG K+RAGR ITS ++SLL  EA+ G L++   E    +  + E+E
Subjt:  VKNHPVKAKPEDQPGIIILAK--------------VLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDE

Sequences

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CDS sequence

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ATGCAACGGATGGAAATGAAAATTGAGGAAGGGTTGATTCGAGAGCTCTGCCTGGAAGCTTCAGAGGTAAAAAACCACCCAGTAAAAGCTAAACCAGAAGATCAGCCAGG
AATTATTATTCTTGCCAAGGTCTTGAAGGCACAAGCAAGGAAATTTGCACGCTTCCTTCCAAACCCTGAAAGACATTGGGGCTCCAAGCTGAGAGCTGGTCGTCAAATCA
CTAGTGTTTACATCTCTCTATTGGATGCAGAGGCAATAATTGGAGCTCTTAGCCATACGGATTACGAAAAGCCTAAAGCACAACGTCCCAAGGTAGAGGATGAATCAGCT
CCCAACCAGTAA

mRNA sequence

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Protein sequence

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MQRMEMKIEEGLIRELCLEASEVKNHPVKAKPEDQPGIIILAKVLKAQARKFARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEKPKAQRPKVEDESA
PNQ

Carg02209 (gene) of Silver-seed gourd (SMH-JMG-627) v2 genome