CuGenDBv2

Gene ID	Carg02696
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Sacchrp_dh_NADP domain-containing protein
Genome location	Carg_Chr02:7319443..7322330
RNA-Seq Expression	Carg02696
Synteny	Carg02696
Gene Ontology terms	GO:0006694 - steroid biosynthetic process (biological process) GO:0016020 - membrane (cellular component) GO:0003854 - 3-beta-hydroxy-delta5-steroid dehydrogenase activity (molecular function)
InterPro domains	IPR002225 - 3-beta hydroxysteroid dehydrogenase/isomerase IPR036291 - NAD(P)-binding domain superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6570406.1 hypothetical protein SDJN03_29321, partial [Cucurbita argyrosperma subsp. sororia]

7.8e-50

59.55

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Query:  MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEA
        MAGA   LKSFSP+AM  ADTEL +QLPQN  N RVLVLGGTGRVG S A AL KF   L ++ G      EKG AMVAT+GRNSRFVEVD EN K LEA
Subjt:  MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEA

Query:  ALRGVNPVVHIAGSFQQTEKCTPTLV---FHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGR---------------------------ASNGGAGPS
        ALR V+ VVH AG FQQTEKCT   V     +NYSQNAK+FKNKA+EANIPAITTA I                                 A  GGAGP+
Subjt:  ALRGVNPVVHIAGSFQQTEKCTPTLV---FHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGR---------------------------ASNGGAGPS

Query:  LLATSFLLLGEKVVAYNKDE
        +LATSFLLLGE+VVAYNK E
Subjt:  LLATSFLLLGEKVVAYNKDE

KAG6605927.1 hypothetical protein SDJN03_03244, partial [Cucurbita argyrosperma subsp. sororia]

6.1e-71

74.77

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Query:  MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRG
        MAGALKSFSPIAMINADTELLVQLPQNDCNP VLVLGGTGRVGTSIAIALFKFSSLAV+TGAACCETEK AAMVATVGRNSRFVEVDAENVKSLEAALRG
Subjt:  MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRG

Query:  VNPVVHIAGSFQQTEKCTPTLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGRAS---------NGGAGPSLLATSFLLLGEKVVAYNKDEATSWI
        V+PVVHIAGSFQQTEKCT + +FH                             NGGRAS         NGGAGPSLLATSFLLLGEKVVAYNK       
Subjt:  VNPVVHIAGSFQQTEKCTPTLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGRAS---------NGGAGPSLLATSFLLLGEKVVAYNKDEATSWI

Query:  AGDEKESRVVYVRM
         GDEKESRVVYVRM
Subjt:  AGDEKESRVVYVRM

KAG7010280.1 hypothetical protein SDJN02_27073, partial [Cucurbita argyrosperma subsp. argyrosperma]

2.5e-48

58.48

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Query:  MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEA
        MAGA   LKSFSP+AM  ADTEL +QLPQN  N RVLVLGGTGRVG S A AL KF   L ++ G      EKG AMVAT+GRNSRFVEVD EN K LEA
Subjt:  MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEA

Query:  ALRGVNP----VVHIAGSFQQTEKCTPTLV---FHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGR---------------------------ASNGG
        ALR V      VVH AG FQQTEKCT   V     +NYSQNAK+FKNKA+EANIPAITTA I                                 A  GG
Subjt:  ALRGVNP----VVHIAGSFQQTEKCTPTLV---FHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGR---------------------------ASNGG

Query:  AGPSLLATSFLLLGEKVVAYNKDE
        AGP++LATSFLLLGE+VVAYNK E
Subjt:  AGPSLLATSFLLLGEKVVAYNKDE

KAG7035876.1 hypothetical protein SDJN02_02675 [Cucurbita argyrosperma subsp. argyrosperma]

1.1e-107

100

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Query:  MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRG
        MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRG
Subjt:  MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRG

Query:  VNPVVHIAGSFQQTEKCTPTLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGRASNGGAGPSLLATSFLLLGEKVVAYNKDEATSWIAGDEKESRV
        VNPVVHIAGSFQQTEKCTPTLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGRASNGGAGPSLLATSFLLLGEKVVAYNKDEATSWIAGDEKESRV
Subjt:  VNPVVHIAGSFQQTEKCTPTLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGRASNGGAGPSLLATSFLLLGEKVVAYNKDEATSWIAGDEKESRV

Query:  VYVRM
        VYVRM
Subjt:  VYVRM

XP_022957944.1 uncharacterized protein LOC111459328 [Cucurbita moschata]

8.3e-52

94.07

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Query:  MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRG
        MAGALKSFSPIAMIN DTELLVQLPQNDCN RVLVLG TGRVGTSIAIALFKFSSLAV+TGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALR 
Subjt:  MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRG

Query:  VNPVVHIAGSFQQTEKCT
        V+PVVH AGSFQQTEKCT
Subjt:  VNPVVHIAGSFQQTEKCT

TrEMBL top hits

e value

%identity

Alignment

A0A6J1DG83 uncharacterized protein LOC111020567 isoform X1

2.5e-46

56.77

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Query:  MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEA
        MAG+   LKSFSP+AM  A+TEL +QLPQN  N RVLVLGGTGRVG S AIAL +F   L +  G      EKGAAMVAT+G NSRFVEVD ENVKSLEA
Subjt:  MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEA

Query:  ALRGVNPVVHIAGSFQQTEKCT------------PTLVFHTNYSQNAKAFKNKAMEANIPAITTAFI-----------LDNGGR----------------
        ALR V+ VVH AG FQQT+KCT              +   TNYS NAKA KNKA++ANIPAITTA I           L    R                
Subjt:  ALRGVNPVVHIAGSFQQTEKCT------------PTLVFHTNYSQNAKAFKNKAMEANIPAITTAFI-----------LDNGGR----------------

Query:  ASNGGAGPSLLATSFLLLGEKVVAYNKDE
        A  GGAGP++LATSFLLLGE+VVAYNK E
Subjt:  ASNGGAGPSLLATSFLLLGEKVVAYNKDE

A0A6J1DHK9 uncharacterized protein LOC111020567 isoform X2

2.5e-46

56.77

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Query:  MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEA
        MAG+   LKSFSP+AM  A+TEL +QLPQN  N RVLVLGGTGRVG S AIAL +F   L +  G      EKGAAMVAT+G NSRFVEVD ENVKSLEA
Subjt:  MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEA

Query:  ALRGVNPVVHIAGSFQQTEKCT------------PTLVFHTNYSQNAKAFKNKAMEANIPAITTAFI-----------LDNGGR----------------
        ALR V+ VVH AG FQQT+KCT              +   TNYS NAKA KNKA++ANIPAITTA I           L    R                
Subjt:  ALRGVNPVVHIAGSFQQTEKCT------------PTLVFHTNYSQNAKAFKNKAMEANIPAITTAFI-----------LDNGGR----------------

Query:  ASNGGAGPSLLATSFLLLGEKVVAYNKDE
        A  GGAGP++LATSFLLLGE+VVAYNK E
Subjt:  ASNGGAGPSLLATSFLLLGEKVVAYNKDE

A0A6J1FYX2 uncharacterized protein LOC111449104

4.6e-48

56.33

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Query:  MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEA
        MAGA   LKSFSP+AM  ADTEL +QLP+N  N RVLVLGGTGRVG S A AL KF   L ++ G      EKG AMVAT+GRNSRFVEVD EN K LEA
Subjt:  MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEA

Query:  ALRGVNPVVHIAGSFQQTEKCT------------PTLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGR---------------------------
        ALR V+ VVH AG FQQTEKCT              +   +NYSQNAK+FKNKA+EANIPAITTA I                                 
Subjt:  ALRGVNPVVHIAGSFQQTEKCT------------PTLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGR---------------------------

Query:  ASNGGAGPSLLATSFLLLGEKVVAYNKDE
        A  GGAGP++LATSFLLLGE+VVAYNK E
Subjt:  ASNGGAGPSLLATSFLLLGEKVVAYNKDE

A0A6J1H1Q4 uncharacterized protein LOC111459328

4.0e-52

94.07

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Query:  MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRG
        MAGALKSFSPIAMIN DTELLVQLPQNDCN RVLVLG TGRVGTSIAIALFKFSSLAV+TGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALR 
Subjt:  MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRG

Query:  VNPVVHIAGSFQQTEKCT
        V+PVVH AGSFQQTEKCT
Subjt:  VNPVVHIAGSFQQTEKCT

A0A6J1J8E6 uncharacterized protein LOC111484409

1.8e-47

55.9

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Query:  MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEA
        MAGA   LKSFSP+AM  ADTEL +QLPQN  N RVLVLGGTGRVG S A AL KF   L ++ G      EKG AMVAT+GRNSRFVEVD EN K LEA
Subjt:  MAGA---LKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEA

Query:  ALRGVNPVVHIAGSFQQTEKCTP------------TLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGR---------------------------
        ALR V+ VVH AG FQQTEKCT              +   + YSQNAK+FKNKA++ANIPAITTA I                                 
Subjt:  ALRGVNPVVHIAGSFQQTEKCTP------------TLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGR---------------------------

Query:  ASNGGAGPSLLATSFLLLGEKVVAYNKDE
        A  GGAGP++LATSFLLLGE+VVAYNK E
Subjt:  ASNGGAGPSLLATSFLLLGEKVVAYNKDE

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G50450.1 Saccharopine dehydrogenase

4.4e-27

43.15

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Query:  NPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEKCTP---------
        N RVLVLGGTGRVG S A AL K    L +  G      EKG AMVA +G NS F +VD  + K LE +LR V+ VVH AG FQQ  +CT          
Subjt:  NPRVLVLGGTGRVGTSIAIALFKF-SSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGSFQQTEKCTP---------

Query:  ---TLVFHTNYSQNAKAFKNKAMEANIPAITTAFIL------------------DNGG---------RASNGGAGPSLLATSFLLLGEKVVAYNKDE
            +   T+Y+  AK+ + +A+ ANIPA+TTA I                   D G           A  GGAGP++LATSFLLLGE+V AY + E
Subjt:  ---TLVFHTNYSQNAKAFKNKAMEANIPAITTAFIL------------------DNGG---------RASNGGAGPSLLATSFLLLGEKVVAYNKDE

Sequences

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CDS sequence

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ATGGCGGGAGCTTTGAAAAGCTTTTCTCCGATAGCCATGATCAATGCCGACACTGAACTTCTGGTTCAATTGCCTCAGAATGATTGCAATCCTAGGGTTTTGGTACTCGG
AGGCACCGGTCGGGTCGGAACCTCCATTGCCATTGCTCTCTTCAAGTTCTCGTCATTGGCGGTCTCAACAGGTGCGGCGTGCTGTGAAACGGAAAAAGGTGCTGCTATGG
TTGCGACAGTTGGGAGAAACTCTCGATTTGTTGAAGTCGATGCTGAGAATGTAAAATCATTGGAGGCTGCTTTGAGAGGTGTGAATCCTGTAGTTCATATTGCTGGGTCA
TTCCAACAGACAGAGAAGTGCACCCCTACGTTGGTGTTTCATACAAACTATTCACAGAATGCAAAAGCTTTCAAGAATAAAGCTATGGAGGCAAATATTCCAGCTATTAC
AACTGCATTTATCCTGGATAATGGTGGCAGAGCTAGCAATGGTGGTGCTGGTCCATCTTTATTAGCTACTAGTTTCTTGCTGCTGGGAGAGAAGGTGGTCGCCTATAATA
AAGATGAGGCTACTTCATGGATAGCAGGTGACGAGAAGGAAAGTCGTGTAGTTTATGTAAGAATGTGA

mRNA sequence

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CTTGCCGGTGCGGAGAAGAGCGGCAAACAATGGCGGGAGCTTTGAAAAGCTTTTCTCCGATAGCCATGATCAATGCCGACACTGAACTTCTGGTTCAATTGCCTCAGAAT
GATTGCAATCCTAGGGTTTTGGTACTCGGAGGCACCGGTCGGGTCGGAACCTCCATTGCCATTGCTCTCTTCAAGTTCTCGTCATTGGCGGTCTCAACAGGTGCGGCGTG
CTGTGAAACGGAAAAAGGTGCTGCTATGGTTGCGACAGTTGGGAGAAACTCTCGATTTGTTGAAGTCGATGCTGAGAATGTAAAATCATTGGAGGCTGCTTTGAGAGGTG
TGAATCCTGTAGTTCATATTGCTGGGTCATTCCAACAGACAGAGAAGTGCACCCCTACGTTGGTGTTTCATACAAACTATTCACAGAATGCAAAAGCTTTCAAGAATAAA
GCTATGGAGGCAAATATTCCAGCTATTACAACTGCATTTATCCTGGATAATGGTGGCAGAGCTAGCAATGGTGGTGCTGGTCCATCTTTATTAGCTACTAGTTTCTTGCT
GCTGGGAGAGAAGGTGGTCGCCTATAATAAAGATGAGGCTACTTCATGGATAGCAGGTGACGAGAAGGAAAGTCGTGTAGTTTATGTAAGAATGTGA

Protein sequence

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MAGALKSFSPIAMINADTELLVQLPQNDCNPRVLVLGGTGRVGTSIAIALFKFSSLAVSTGAACCETEKGAAMVATVGRNSRFVEVDAENVKSLEAALRGVNPVVHIAGS
FQQTEKCTPTLVFHTNYSQNAKAFKNKAMEANIPAITTAFILDNGGRASNGGAGPSLLATSFLLLGEKVVAYNKDEATSWIAGDEKESRVVYVRM

Carg02696 (gene) of Silver-seed gourd (SMH-JMG-627) v2 genome