CuGenDBv2

Gene ID	Carg03011
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Transcription initiation factor IIA subunit 2
Genome location	Carg_Chr09:2981286..2983097
RNA-Seq Expression	Carg03011
Synteny	Carg03011
Gene Ontology terms	GO:0006413 - translational initiation (biological process) GO:0051123 - RNA polymerase II preinitiation complex assembly (biological process) GO:0005672 - transcription factor TFIIA complex (cellular component) GO:0003743 - translation initiation factor activity (molecular function) GO:0016251 - RNA polymerase II general transcription initiation factor activity (molecular function) GO:0017025 - TBP-class protein binding (molecular function)
InterPro domains	IPR003194 - Transcription initiation factor IIA, gamma subunit IPR009083 - Transcription factor IIA, alpha-helical domain IPR009088 - Transcription factor IIA, beta-barrel IPR015871 - Transcription initiation factor IIA, gamma subunit, C-terminal IPR015872 - Transcription initiation factor IIA, gamma subunit, N-terminal

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6608125.1 Transcription initiation factor IIA subunit 2, partial [Cucurbita argyrosperma subsp. sororia]

3.6e-51

99.06

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKV+IKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLLTQ
Subjt:  SKLLTQ

KAG7031763.1 Transcription initiation factor IIA subunit 2 [Cucurbita argyrosperma subsp. argyrosperma]

4.8e-51

98.11

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKV++KGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLLTQ
Subjt:  SKLLTQ

XP_022133819.1 transcription initiation factor IIA subunit 2 [Momordica charantia]

1.8e-50

98.11

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQE VGRIKIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLL+Q
Subjt:  SKLLTQ

XP_022936677.1 transcription initiation factor IIA subunit 2 [Cucurbita moschata]

1.6e-51

100

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLLTQ
Subjt:  SKLLTQ

XP_022941425.1 transcription initiation factor IIA subunit 2-like [Cucurbita moschata]

3.6e-51

99.06

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKV+IKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLLTQ
Subjt:  SKLLTQ

TrEMBL top hits

e value

%identity

Alignment

A0A6J1BWA2 Transcription initiation factor IIA subunit 2

8.7e-51

98.11

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQE VGRIKIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLL+Q
Subjt:  SKLLTQ

A0A6J1F935 Transcription initiation factor IIA subunit 2

7.9e-52

100

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLLTQ
Subjt:  SKLLTQ

A0A6J1FTM1 Transcription initiation factor IIA subunit 2

1.8e-51

99.06

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKV+IKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLLTQ
Subjt:  SKLLTQ

A0A6J1ILT9 Transcription initiation factor IIA subunit 2

7.9e-52

100

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLLTQ
Subjt:  SKLLTQ

A0A6J1J100 Transcription initiation factor IIA subunit 2

1.8e-51

99.06

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKV+IKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLLTQ
Subjt:  SKLLTQ

SwissProt top hits

e value

%identity

Alignment

A2XZI2 Transcription initiation factor IIA subunit 2

3.9e-48

87.74

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMV +GTLSPELAIQVLVQFDKSMTEALE+QVKSKVSIKGHLHTYRFCDNVWTFIL +A+FKNE++ E VG++KIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLL+Q
Subjt:  SKLLTQ

O74948 Transcription initiation factor IIA subunit 2

1.9e-26

52.53

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Query:  FELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACDSK
        +ELYRRS+IG+ LT+ LD+++  G +SP+LA++VL  FDKSMTEAL  +V+S+++ KGHL TYRFCD VWTFI+++ +F+ ++   T  +I+IVAC ++
Subjt:  FELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACDSK

Q0DLD3 Transcription initiation factor IIA subunit 2

3.9e-48

87.74

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMV +GTLSPELAIQVLVQFDKSMTEALE+QVKSKVSIKGHLHTYRFCDNVWTFIL +A+FKNE++ E VG++KIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLL+Q
Subjt:  SKLLTQ

Q39236 Transcription initiation factor IIA subunit 2

7.1e-50

91.51

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQ+GTLSPELAIQVLVQFDKSMTEALESQVK+KVSIKGHLHTYRFCDNVWTFILQDA FK++D QE V R+KIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLLTQ
Subjt:  SKLLTQ

Q90YG6 Transcription initiation factor IIA subunit 2

4.7e-25

50.51

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Query:  FELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACDSK
        ++LYR +T+G  L E+LDE++Q   ++P+LA+QVL+QFDK++  AL ++V+++V+ KG L+TYRFCDNVWTF+L D  F+       V ++KIVACD K
Subjt:  FELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACDSK

Arabidopsis top hits

e value

%identity

Alignment

AT4G24440.1 transcription initiation factor IIA gamma chain / TFIIA-gamma (TFIIA-S)

5.1e-51

91.51

Show/hide

Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQ+GTLSPELAIQVLVQFDKSMTEALESQVK+KVSIKGHLHTYRFCDNVWTFILQDA FK++D QE V R+KIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLLTQ
Subjt:  SKLLTQ

AT4G24440.2 transcription initiation factor IIA gamma chain / TFIIA-gamma (TFIIA-S)

5.1e-51

91.51

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Query:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD
        MATFELYRRSTIGMCLTETLDEMVQ+GTLSPELAIQVLVQFDKSMTEALESQVK+KVSIKGHLHTYRFCDNVWTFILQDA FK++D QE V R+KIVACD
Subjt:  MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACD

Query:  SKLLTQ
        SKLLTQ
Subjt:  SKLLTQ

Sequences

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CDS sequence

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ATGGCAACGTTTGAACTCTACCGTAGATCGACGATCGGAATGTGTTTGACTGAAACTTTGGACGAAATGGTCCAGAACGGTACTCTTAGTCCGGAGCTTGCTATTCAAGT
TCTTGTCCAGTTCGATAAGTCCATGACTGAGGCTCTGGAATCACAGGTGAAAAGCAAGGTGTCTATCAAGGGACATTTGCACACCTATCGGTTCTGCGACAATGTTTGGA
CATTCATTTTGCAGGATGCAACATTTAAGAACGAGGATTCCCAAGAGACTGTTGGAAGAATTAAAATAGTAGCATGTGACTCAAAGTTACTCACACAATAA

mRNA sequence

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AAATTTCTTCGCTCGCGAAAAGCTTTGGAGCCATCGTTGTCCCGGTTAGAATCAAGAAACGAGAAGAATCACATACTTACCAACAATCTATCTTGTTTTCTGAAGAAGAA
TGGCAACGTTTGAACTCTACCGTAGATCGACGATCGGAATGTGTTTGACTGAAACTTTGGACGAAATGGTCCAGAACGGTACTCTTAGTCCGGAGCTTGCTATTCAAGTT
CTTGTCCAGTTCGATAAGTCCATGACTGAGGCTCTGGAATCACAGGTGAAAAGCAAGGTGTCTATCAAGGGACATTTGCACACCTATCGGTTCTGCGACAATGTTTGGAC
ATTCATTTTGCAGGATGCAACATTTAAGAACGAGGATTCCCAAGAGACTGTTGGAAGAATTAAAATAGTAGCATGTGACTCAAAGTTACTCACACAATAAGCTTGATGAT
GAAATTTGCATAATTATTTGATGATGGTAGAAGGAAATAGTCATGGTGACGAAATGGCCATCCGGGCAGTGTTGCGAGTTGGAAGCGATTCAAACATGATTCTGTAAAGT
CATCGACCCGAACAGCTAAAAGTTACTTTCACGCCCATTGACGATACATTTGACTGTAATTGGCATTTAAAGCTGATTACATAACAATTATACAGCCCTTCTAGTGTAAT
GATAATAAAAACATGATATCTATTTTCATATT

Protein sequence

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MATFELYRRSTIGMCLTETLDEMVQNGTLSPELAIQVLVQFDKSMTEALESQVKSKVSIKGHLHTYRFCDNVWTFILQDATFKNEDSQETVGRIKIVACDSKLLTQ

Carg03011 (gene) of Silver-seed gourd (SMH-JMG-627) v2 genome