CuGenDBv2

Gene ID	Carg03738
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	splicing factor 3A subunit 2
Genome location	Carg_Chr14:1020167..1021510
RNA-Seq Expression	Carg03738
Synteny	Carg03738
Gene Ontology terms	GO:0000245 - spliceosomal complex assembly (biological process) GO:0005686 - U2 snRNP (cellular component) GO:0071004 - U2-type prespliceosome (cellular component) GO:0071013 - catalytic step 2 spliceosome (cellular component) GO:0003676 - nucleic acid binding (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6580632.1 hypothetical protein SDJN03_20634, partial [Cucurbita argyrosperma subsp. sororia]

6.1e-69

81.87

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Query:  MRVITWRIRRGSGIRLILAKRAAHEAKEASA--QPQPHKRKFNLVNN-------------------------KLQLYFKSKPQPVPAANGTVPPPPQAPP
        MRVITWRIRRGSGIRLILAKRAAHEAKEASA  QPQPHKRK ++                            +LQLYFKSKPQPVPAANGTVPPPPQAPP
Subjt:  MRVITWRIRRGSGIRLILAKRAAHEAKEASA--QPQPHKRKFNLVNN-------------------------KLQLYFKSKPQPVPAANGTVPPPPQAPP

Query:  PPPPSPQQGVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
        PPPP PQQGVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
Subjt:  PPPPSPQQGVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG

KAG7017390.1 hypothetical protein SDJN02_19255, partial [Cucurbita argyrosperma subsp. argyrosperma]

1.7e-79

100

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Query:  MRVITWRIRRGSGIRLILAKRAAHEAKEASAQPQPHKRKFNLVNNKLQLYFKSKPQPVPAANGTVPPPPQAPPPPPPSPQQGVPPRVPPPMPGSLPPPPS
        MRVITWRIRRGSGIRLILAKRAAHEAKEASAQPQPHKRKFNLVNNKLQLYFKSKPQPVPAANGTVPPPPQAPPPPPPSPQQGVPPRVPPPMPGSLPPPPS
Subjt:  MRVITWRIRRGSGIRLILAKRAAHEAKEASAQPQPHKRKFNLVNNKLQLYFKSKPQPVPAANGTVPPPPQAPPPPPPSPQQGVPPRVPPPMPGSLPPPPS

Query:  VMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
        VMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
Subjt:  VMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG

XP_004137848.1 splicing factor 3A subunit 2 [Cucumis sativus]

6.7e-44

82.35

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Query:  LQLYFKSKPQ------PVPAANGTV---------PPPPQAPPPPPPSPQQGVPPRV-PPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMA
        LQLYFKSKPQ      PVPAANGTV         PPPPQAPPPPPP PQQG PPRV PPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPP+GNNTMA
Subjt:  LQLYFKSKPQ------PVPAANGTV---------PPPPQAPPPPPPSPQQGVPPRV-PPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMA

Query:  NFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
        NFTPG+ +N PPMPPPPQGFPGQG+RQPPPPPPNMG
Subjt:  NFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG

XP_022934763.1 formin-like protein 20 isoform X1 [Cucurbita moschata]

1.9e-70

82.72

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Query:  MRVITWRIRRGSGIRLILAKRAAHEAKEASAQPQPHKRKFNLVNN-------------------------KLQLYFKSKPQPVPAANGTVPPPPQAPPPP
        MRVITWRIRRGSGIRLILAKRAAHEAKEASAQPQPHKRK ++                            +LQLYFKSKPQPVPAANGTVPPPPQAPPPP
Subjt:  MRVITWRIRRGSGIRLILAKRAAHEAKEASAQPQPHKRKFNLVNN-------------------------KLQLYFKSKPQPVPAANGTVPPPPQAPPPP

Query:  PPSPQQGVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
        PP PQQGVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
Subjt:  PPSPQQGVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG

XP_022934764.1 formin-like protein 20 isoform X2 [Cucurbita moschata]

1.4e-65

81.08

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Query:  RIRRGSGIRLILAKRAAHEAKEASAQPQPHKRKFNLVNN-------------------------KLQLYFKSKPQPVPAANGTVPPPPQAPPPPPPSPQQ
        R+ RGSGIRLILAKRAAHEAKEASAQPQPHKRK ++                            +LQLYFKSKPQPVPAANGTVPPPPQAPPPPPP PQQ
Subjt:  RIRRGSGIRLILAKRAAHEAKEASAQPQPHKRKFNLVNN-------------------------KLQLYFKSKPQPVPAANGTVPPPPQAPPPPPPSPQQ

Query:  GVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
        GVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
Subjt:  GVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LEB1 Matrin-type domain-containing protein

3.3e-44

82.35

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Query:  LQLYFKSKPQ------PVPAANGTV---------PPPPQAPPPPPPSPQQGVPPRV-PPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMA
        LQLYFKSKPQ      PVPAANGTV         PPPPQAPPPPPP PQQG PPRV PPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPP+GNNTMA
Subjt:  LQLYFKSKPQ------PVPAANGTV---------PPPPQAPPPPPPSPQQGVPPRV-PPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMA

Query:  NFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
        NFTPG+ +N PPMPPPPQGFPGQG+RQPPPPPPNMG
Subjt:  NFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG

A0A5A7TQW8 Splicing factor 3A subunit 2

1.2e-43

82.35

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Query:  LQLYFKSKPQ------PVPAANGTV---------PPPPQAPPPPPPSPQQGVPPRV-PPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMA
        LQLYFKSKPQ      PVPAANGTV         PPPPQAPPPPPP PQQG PPRV PPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMA
Subjt:  LQLYFKSKPQ------PVPAANGTV---------PPPPQAPPPPPPSPQQGVPPRV-PPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMA

Query:  NFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
        NFTPG+ +N PPMPPPPQ FPGQG+RQPPPPPPNMG
Subjt:  NFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG

A0A5D3DPC7 Splicing factor 3A subunit 2

1.2e-43

82.35

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Query:  LQLYFKSKPQ------PVPAANGTV---------PPPPQAPPPPPPSPQQGVPPRV-PPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMA
        LQLYFKSKPQ      PVPAANGTV         PPPPQAPPPPPP PQQG PPRV PPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMA
Subjt:  LQLYFKSKPQ------PVPAANGTV---------PPPPQAPPPPPPSPQQGVPPRV-PPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMA

Query:  NFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
        NFTPG+ +N PPMPPPPQ FPGQG+RQPPPPPPNMG
Subjt:  NFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG

A0A6J1F3I1 formin-like protein 20 isoform X2

6.8e-66

81.08

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Query:  RIRRGSGIRLILAKRAAHEAKEASAQPQPHKRKFNLVNN-------------------------KLQLYFKSKPQPVPAANGTVPPPPQAPPPPPPSPQQ
        R+ RGSGIRLILAKRAAHEAKEASAQPQPHKRK ++                            +LQLYFKSKPQPVPAANGTVPPPPQAPPPPPP PQQ
Subjt:  RIRRGSGIRLILAKRAAHEAKEASAQPQPHKRKFNLVNN-------------------------KLQLYFKSKPQPVPAANGTVPPPPQAPPPPPPSPQQ

Query:  GVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
        GVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
Subjt:  GVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG

A0A6J1F8K3 formin-like protein 20 isoform X1

9.1e-71

82.72

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Query:  MRVITWRIRRGSGIRLILAKRAAHEAKEASAQPQPHKRKFNLVNN-------------------------KLQLYFKSKPQPVPAANGTVPPPPQAPPPP
        MRVITWRIRRGSGIRLILAKRAAHEAKEASAQPQPHKRK ++                            +LQLYFKSKPQPVPAANGTVPPPPQAPPPP
Subjt:  MRVITWRIRRGSGIRLILAKRAAHEAKEASAQPQPHKRKFNLVNN-------------------------KLQLYFKSKPQPVPAANGTVPPPPQAPPPP

Query:  PPSPQQGVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
        PP PQQGVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG
Subjt:  PPSPQQGVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG

SwissProt top hits

e value

%identity

Alignment

Q54WH2 Formin-A

7.1e-04

43.65

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Query:  SKPQPVPAANGTVPPPP--------QAPPPPPPSPQQG----VPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNG
        +KP    A+    PPPP          PPPPPP P  G     PP  PPPM G  PPPP      PP P   GG PP PPPPP G        PG+   G
Subjt:  SKPQPVPAANGTVPPPP--------QAPPPPPPSPQQG----VPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGAPPIPPPPPVGNNTMANFTPGSLVNG

Query:  PPMPPPPQGFPGQGVRQPPPPPPNMG
        PP PPPP G      + PPPPP   G
Subjt:  PPMPPPPQGFPGQGVRQPPPPPPNMG

Q84ZL0 Formin-like protein 5

1.3e-05

46.83

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Query:  PQPVPAANGTVPPPP--------QAPPPPPPSP--QQGVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGA---PPIPPPPPVGNNTMANF-TPGSLVNG
        P P+PAA    PPPP         APPPPPP P  + G PP  PPP     PPPP     GPP P PP GA   PP PPPPP G  +      PG   + 
Subjt:  PQPVPAANGTVPPPP--------QAPPPPPPSP--QQGVPPRVPPPMPGSLPPPPSVMANGPPRPMPPGGA---PPIPPPPPVGNNTMANF-TPGSLVNG

Query:  PPMPPPPQGFPGQGVRQPPPPPPNMG
        PP PPPP    G     PPPPPP  G
Subjt:  PPMPPPPQGFPGQGVRQPPPPPPNMG

Q9FLQ7 Formin-like protein 20

1.3e-05

46.88

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Query:  PQPVPAANGTVPPPP-------QAPPPPPPSPQQGVPPRVPPPMP--GSLPPPP----SVMANGPPRPMPP---GGAPPIPPPPPVGNNTMANFTPGSLV
        P P P + G+ PPPP         PPPPPP P  G PP  PPP P  GS PPPP    S +++ PP P PP   GGAPP PPPPP+         P  + 
Subjt:  PQPVPAANGTVPPPP-------QAPPPPPPSPQQGVPPRVPPPMP--GSLPPPP----SVMANGPPRPMPP---GGAPPIPPPPPVGNNTMANFTPGSLV

Query:  NGPPMPPPPQGFPGQGVRQPPPPPPNMG
         G P PPPP   P  G   PPPPPP  G
Subjt:  NGPPMPPPPQGFPGQGVRQPPPPPPNMG

Arabidopsis top hits

e value

%identity

Alignment

AT1G61080.1 Hydroxyproline-rich glycoprotein family protein

3.0e-05

43.48

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Query:  PQPVPAA----NGTVPPPP---------QAPPPPPPSPQQGVPPRVPPPMPGSL------PPPPSVMANGPPRPMPP-GGAPPIPPPPPVGNNTMANFTP
        P P P A     G+ PPPP          APPPPPP P+  V P  PPP PG+       PPPP   A  PP P PP     P PPP P+GN+      P
Subjt:  PQPVPAA----NGTVPPPP---------QAPPPPPPSPQQGVPPRVPPPMPGSL------PPPPSVMANGPPRPMPP-GGAPPIPPPPPVGNNTMANFTP

Query:  G----SLVNG--PPMPPPPQGFPGQGVRQPPPPPPNMG
              L NG  PP PPPP      G   PPPPPP MG
Subjt:  G----SLVNG--PPMPPPPQGFPGQGVRQPPPPPPNMG

AT5G07740.1 actin binding

9.2e-07

46.88

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Query:  PQPVPAANGTVPPPP-------QAPPPPPPSPQQGVPPRVPPPMP--GSLPPPP----SVMANGPPRPMPP---GGAPPIPPPPPVGNNTMANFTPGSLV
        P P P + G+ PPPP         PPPPPP P  G PP  PPP P  GS PPPP    S +++ PP P PP   GGAPP PPPPP+         P  + 
Subjt:  PQPVPAANGTVPPPP-------QAPPPPPPSPQQGVPPRVPPPMP--GSLPPPP----SVMANGPPRPMPP---GGAPPIPPPPPVGNNTMANFTPGSLV

Query:  NGPPMPPPPQGFPGQGVRQPPPPPPNMG
         G P PPPP   P  G   PPPPPP  G
Subjt:  NGPPMPPPPQGFPGQGVRQPPPPPPNMG

Sequences

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CDS sequence

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ATGAGGGTAATTACTTGGCGCATACGCAGGGGAAGCGGCATCAGACTAATTTTGGCTAAGAGAGCTGCCCATGAAGCCAAGGAGGCTTCTGCTCAACCTCAACCCCACAA
GCGGAAGTTTAATCTTGTTAACAACAAATTGCAGTTGTATTTCAAATCCAAGCCACAACCTGTGCCTGCAGCCAACGGAACAGTGCCGCCACCACCACAAGCTCCACCGC
CACCGCCACCTTCACCCCAGCAAGGTGTACCACCAAGAGTTCCTCCTCCGATGCCTGGATCCTTGCCACCTCCCCCATCTGTAATGGCCAATGGTCCTCCCAGGCCAATG
CCTCCTGGTGGAGCTCCGCCGATACCCCCACCGCCTCCAGTTGGTAATAACACAATGGCAAATTTCACTCCTGGTTCTCTGGTGAATGGACCACCCATGCCGCCGCCTCC
ACAGGGCTTCCCAGGCCAGGGTGTCCGGCAGCCGCCACCACCGCCACCCAATATGGGATAG

mRNA sequence

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Protein sequence

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MRVITWRIRRGSGIRLILAKRAAHEAKEASAQPQPHKRKFNLVNNKLQLYFKSKPQPVPAANGTVPPPPQAPPPPPPSPQQGVPPRVPPPMPGSLPPPPSVMANGPPRPM
PPGGAPPIPPPPPVGNNTMANFTPGSLVNGPPMPPPPQGFPGQGVRQPPPPPPNMG

Carg03738 (gene) of Silver-seed gourd (SMH-JMG-627) v2 genome