CuGenDBv2

Gene ID	Carg07857
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Leucyl-tRNA synthetase
Genome location	Carg_Chr07:1468885..1469335
RNA-Seq Expression	Carg07857
Synteny	Carg07857
Gene Ontology terms	GO:0006429 - leucyl-tRNA aminoacylation (biological process) GO:0009793 - embryo development ending in seed dormancy (biological process) GO:0106074 - aminoacyl-tRNA metabolism involved in translational fidelity (biological process) GO:0005739 - mitochondrion (cellular component) GO:0005829 - cytosol (cellular component) GO:0009507 - chloroplast (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0002161 - aminoacyl-tRNA editing activity (molecular function) GO:0004823 - leucine-tRNA ligase activity (molecular function) GO:0005524 - ATP binding (molecular function)
InterPro domains	IPR002302 - Leucine-tRNA ligase IPR009080 - Aminoacyl-tRNA synthetase, class Ia, anticodon-binding IPR013155 - Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6603795.1 Leucine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia]

4.0e-23

80.26

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE
        +VTEEVEGTRFNTGISA MEFVN+AY+W     DRYPRSIVEAFTLLLS YAPHLAEEL S LGHSE LAYE FP+
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE

KAG7026646.1 Leucine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma]

1.4e-36

100

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Query:  FQVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPEV
        FQVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPEV
Subjt:  FQVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPEV

KAG7033965.1 Leucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma]

4.0e-23

80.26

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE
        +VTEEVEGTRFNTGISA MEFVN+AY+W     DRYPRSIVEAFTLLLS YAPHLAEEL S LGHSE LAYE FP+
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE

XP_022949941.1 leucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata]

4.0e-23

80.26

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE
        +VTEEVEGTRFNTGISA MEFVN+AY+W     DRYPRSIVEAFTLLLS YAPHLAEEL S LGHSE LAYE FP+
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE

XP_023544274.1 leucine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo]

4.0e-23

80.26

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE
        +VTEEVEGTRFNTGISA MEFVN+AY+W     DRYPRSIVEAFTLLLS YAPHLAEEL S LGHSE LAYE FP+
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KL57 Leucyl-tRNA synthetase

4.3e-23

78.95

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE
        +VTEEVEGTRFNTGISA MEFVN+AY+W     DRYPR+IVEAFTLLLS YAPHLAEEL S LGHSE LAYE FP+
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE

A0A1S3B071 Leucyl-tRNA synthetase

2.2e-22

77.63

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE
        +VTEEVEGTRFNTGISA MEFVN+AY+W     D YPR+IVEAFTLLLS YAPHLAEEL S LGHSE LAYE FP+
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE

A0A5D3CM74 Leucyl-tRNA synthetase

2.2e-22

77.63

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE
        +VTEEVEGTRFNTGISA MEFVN+AY+W     D YPR+IVEAFTLLLS YAPHLAEEL S LGHSE LAYE FP+
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE

A0A6J1GDF8 Leucyl-tRNA synthetase

2.0e-23

80.26

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE
        +VTEEVEGTRFNTGISA MEFVN+AY+W     DRYPRSIVEAFTLLLS YAPHLAEEL S LGHSE LAYE FP+
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE

A0A6J1ILI7 Leucyl-tRNA synthetase

2.0e-23

80.26

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE
        +VTEEVEGTRFNTGISA MEFVN+AY+W     DRYPRSIVEAFTLLLS YAPHLAEEL S LGHSE LAYE FP+
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE

SwissProt top hits

e value

%identity

Alignment

A8FGG0 Leucine--tRNA ligase

2.5e-12

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE
        +VT+  EG RFNTGIS  M F+N AY     K D  P+   E F  LLS  APHLAEEL + LGH   ++YE++P+
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE

P36430 Leucine--tRNA ligase

1.5e-12

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFP
        +VT+  EG RFNTGIS  M F+N AY+ T +     P+  +E F  LLS  APHLAEEL   LGHS  +AYE++P
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFP

Q2S415 Leucine--tRNA ligase

1.8e-13

52.7

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Query:  VTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFP
        VTE++E   FNT I+A MEFVN A +W     D  PR +   F LLLS +APHLAEEL + LGH + LAY  +P
Subjt:  VTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFP

Q65FX8 Leucine--tRNA ligase

7.9e-14

53.33

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFP
        +VT+  EG RFNTGIS  M F+N AY     K D+ P+  +E F  LLS  APHLAEEL + LGH E +AYE++P
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFP

Q9XEA0 Leucine--tRNA ligase, chloroplastic/mitochondrial

7.4e-20

64.47

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE
        +VTEE+E TRFNTGIS  MEFVN AY+W     +  PR I+E F LLLS YAPH+AEEL S LGH   LAYESFP+
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE

Arabidopsis top hits

e value

%identity

Alignment

AT4G04350.1 tRNA synthetase class I (I, L, M and V) family protein

5.3e-21

64.47

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Query:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE
        +VTEE+E TRFNTGIS  MEFVN AY+W     +  PR I+E F LLLS YAPH+AEEL S LGH   LAYESFP+
Subjt:  QVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPE

Sequences

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CDS sequence

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TTCCAGGTAACCGAAGAAGTTGAAGGGACACGGTTCAACACAGGAATCTCAGCTACGATGGAGTTCGTTAACATGGCATATAGGTGGACTTACATTAAAAGGGACAGATA
TCCAAGGTCTATTGTTGAAGCATTCACTTTGTTGCTTTCACTGTACGCACCTCACTTGGCTGAGGAACTTTGTTCTTGGTTAGGACACTCAGAATTGTTGGCATACGAGT
CCTTCCCCGAGGTGTGA

mRNA sequence

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Protein sequence

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FQVTEEVEGTRFNTGISATMEFVNMAYRWTYIKRDRYPRSIVEAFTLLLSLYAPHLAEELCSWLGHSELLAYESFPEV

Carg07857 (gene) of Silver-seed gourd (SMH-JMG-627) v2 genome