CuGenDBv2

Gene ID	Carg07898
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DNA-directed RNA polymerases IV and V subunit 4-like
Genome location	Carg_Chr07:1612622..1614281
RNA-Seq Expression	Carg07898
Synteny	Carg07898
Gene Ontology terms	GO:0006352 - DNA-templated transcription, initiation (biological process) GO:0005634 - nucleus (cellular component) GO:0030880 - RNA polymerase complex (cellular component) GO:0000166 - nucleotide binding (molecular function) GO:0003899 - DNA-directed 5'-3' RNA polymerase activity (molecular function) GO:0005509 - calcium ion binding (molecular function)
InterPro domains	IPR010997 - HRDC-like superfamily IPR038324 - Rpb4/RPC9 superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6594716.1 DNA-directed RNA polymerases IV and V subunit 4, partial [Cucurbita argyrosperma subsp. sororia]

7.0e-79

97.01

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
        MSEKGEKGFPVQKKPGKSSLKSSASKD   +SLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE

Query:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        QELPKNVKCQCLMDCEAAQLLQGIQDQM LLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
Subjt:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

KAG7026685.1 DNA-directed RNA polymerases IV and V subunit 4, partial [Cucurbita argyrosperma subsp. argyrosperma]

6.1e-91

100

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Query:  VLLCGQQFSIMSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSK
        VLLCGQQFSIMSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSK
Subjt:  VLLCGQQFSIMSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSK

Query:  EQQPLELKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYP
        EQQPLELKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYP
Subjt:  EQQPLELKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYP

XP_022926857.1 DNA-directed RNA polymerases IV and V subunit 4-like isoform X1 [Cucurbita moschata]

1.2e-78

97.01

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
        MSEKGEKGFPVQKKPGKSSLKSSASKD   +SLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE

Query:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTE VRPVLE
Subjt:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

XP_023003382.1 DNA-directed RNA polymerases IV and V subunit 4-like [Cucurbita maxima]

6.5e-77

94.61

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
        MSEKGEKGFPVQKKPGKSSLKS ASKD   +SLKGKDDSLSKSKKGRKVQFDAQGSVDA INLS+KF GKNGDLGKGGKGINGGKASVSKEQQPLELKIE
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE

Query:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        QELPKNVKCQCLMDCEAAQLLQGIQDQM LLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
Subjt:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

XP_023517145.1 DNA-directed RNA polymerases IV and V subunit 4-like isoform X1 [Cucurbita pepo subsp. pepo]

4.5e-78

96.41

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
        MSEKGEKGFPVQKKPGKSSLKSSASKD   +SLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE

Query:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
         ELPKNVKCQCLMDCEAAQLLQGIQDQM LLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
Subjt:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

TrEMBL top hits

e value

%identity

Alignment

A0A5A7SHG0 Calcineurin B-like protein 3

1.4e-69

84.97

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Query:  QFSIMSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLE
        +FS+MSEKGEKGF VQKKP KSSLKSSA KD   +SLKGKDDSLSK KKGRKVQFDAQGSVDA  + S+K+SGKNGD+GKGGKG N  KASV+KE Q LE
Subjt:  QFSIMSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLE

Query:  LKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYP
        LKIEQELPKNVKCQCLMDCEAAQLLQGIQDQM  LSADPTIKIPTSFDRGLQYAKRANHYVN ESVRPVLEYP
Subjt:  LKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYP

A0A5D3CKP7 Calcineurin B-like protein 3

1.0e-67

83.24

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Query:  QFSIMSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLE
        +FS+MSEKGEKGF VQKKP KSSLKSSA KD       GKDDSLSK KKGRKVQFDAQGSVDA  + S+K+SGKNGD+GKGGKG N  KASV+KE Q LE
Subjt:  QFSIMSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLE

Query:  LKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYP
        LKIEQELPKNVKCQCLMDCEAAQLLQGIQDQM  LSADPTIKIPTSFDRGLQYAKRANHYVN ESVRPVLEYP
Subjt:  LKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYP

A0A6J1EGC4 DNA-directed RNA polymerases IV and V subunit 4-like isoform X2

4.1e-77

95.21

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
        MSEKGEKGFPVQKKPGKSSLKSSASKD       GKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE

Query:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTE VRPVLE
Subjt:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

A0A6J1EJD9 DNA-directed RNA polymerases IV and V subunit 4-like isoform X1

5.8e-79

97.01

Show/hide

Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
        MSEKGEKGFPVQKKPGKSSLKSSASKD   +SLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE

Query:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTE VRPVLE
Subjt:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

A0A6J1KMD1 DNA-directed RNA polymerases IV and V subunit 4-like

3.2e-77

94.61

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
        MSEKGEKGFPVQKKPGKSSLKS ASKD   +SLKGKDDSLSKSKKGRKVQFDAQGSVDA INLS+KF GKNGDLGKGGKGINGGKASVSKEQQPLELKIE
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE

Query:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        QELPKNVKCQCLMDCEAAQLLQGIQDQM LLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
Subjt:  QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

SwissProt top hits

e value

%identity

Alignment

O48890 DNA-directed RNA polymerase II subunit 4

3.3e-07

37.04

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Query:  SVSKEQQPLELKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        S  +E+   ELKI  E    +K +CLM+CE + +L+   +Q+  +S DP  ++   F++ LQY KR + Y N ++VR V E
Subjt:  SVSKEQQPLELKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

Q6DBA5 DNA-directed RNA polymerases IV and V subunit 4

3.2e-18

44.71

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQ---INLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLEL
        MSEKG KG        KSSLK   SKDG      GKD S +K KKGRK+ FD Q +  A    +N+S           K GK     K+S        EL
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQ---INLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLEL

Query:  KIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        K   +LP+N   +C+MDCEA Q+L GI+ Q+  LS DP+IKIP S+DR L Y +   HY N +SVR VLE
Subjt:  KIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

Arabidopsis top hits

e value

%identity

Alignment

AT4G15950.1 RNA polymerase II, Rpb4, core protein

2.3e-19

44.71

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Query:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQ---INLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLEL
        MSEKG KG        KSSLK   SKDG      GKD S +K KKGRK+ FD Q +  A    +N+S           K GK     K+S        EL
Subjt:  MSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQ---INLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLEL

Query:  KIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        K   +LP+N   +C+MDCEA Q+L GI+ Q+  LS DP+IKIP S+DR L Y +   HY N +SVR VLE
Subjt:  KIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

AT5G09920.1 RNA polymerase II, Rpb4, core protein

2.4e-08

37.04

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Query:  SVSKEQQPLELKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE
        S  +E+   ELKI  E    +K +CLM+CE + +L+   +Q+  +S DP  ++   F++ LQY KR + Y N ++VR V E
Subjt:  SVSKEQQPLELKIEQELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLE

Sequences

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CDS sequence

Show/hide CDS sequence

GTTTTGCTTTGCGGTCAACAATTTTCAATAATGTCGGAGAAAGGCGAAAAGGGTTTTCCAGTGCAGAAGAAACCTGGAAAGTCTTCGCTCAAATCTTCGGCTTCCAAGGA
TGGTTCGTATTCTTCTCTAAAAGGAAAGGATGACAGTTTGTCGAAGTCAAAGAAGGGTAGGAAAGTCCAGTTCGATGCTCAAGGGTCAGTTGATGCGCAGATCAATCTTT
CATTGAAATTCAGTGGAAAAAATGGTGACTTGGGTAAAGGAGGGAAAGGTATAAATGGTGGGAAGGCTTCTGTTTCAAAGGAACAACAACCGCTAGAACTGAAGATTGAG
CAAGAACTTCCCAAGAATGTTAAATGCCAATGCCTTATGGACTGTGAGGCTGCACAACTTTTACAGGGAATCCAAGATCAGATGGCTCTTCTATCAGCAGATCCAACCAT
CAAAATCCCTACGTCATTTGATCGGGGACTGCAATATGCTAAACGAGCCAACCACTATGTAAATACCGAGTCGGTTAGACCAGTTCTTGAGTATCCTTGA

mRNA sequence

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Protein sequence

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VLLCGQQFSIMSEKGEKGFPVQKKPGKSSLKSSASKDGSYSSLKGKDDSLSKSKKGRKVQFDAQGSVDAQINLSLKFSGKNGDLGKGGKGINGGKASVSKEQQPLELKIE
QELPKNVKCQCLMDCEAAQLLQGIQDQMALLSADPTIKIPTSFDRGLQYAKRANHYVNTESVRPVLEYP

Carg07898 (gene) of Silver-seed gourd (SMH-JMG-627) v2 genome