CuGenDBv2

Gene ID	Carg09169
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein MIS12 homolog
Genome location	Carg_Chr11:1250355..1253566
RNA-Seq Expression	Carg09169
Synteny	Carg09169
Gene Ontology terms	GO:0000070 - mitotic sister chromatid segregation (biological process) GO:0034501 - protein localization to kinetochore (biological process) GO:0051301 - cell division (biological process) GO:0051382 - kinetochore assembly (biological process) GO:0000818 - nuclear MIS12/MIND complex (cellular component)
InterPro domains	IPR008685 - Centromere protein Mis12

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAG6589575.1 Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia]

2.1e-87

73.95

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Query:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
        MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSN
Subjt:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN

Query:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME
                                        VRKENV LNQE QALER+TASSNSQ N FNEALQLYEQ+SVNEMFQEMMGTAS+LRA+IGKLK+R+ME
Subjt:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME

Query:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL
        +SKLT V+K+HTNGDISHHHKGFSNAKLDDIQEFLA L
Subjt:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL

XP_022931718.1 protein MIS12 homolog isoform X1 [Cucurbita moschata]

1.9e-104

86.67

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Query:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
        MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
Subjt:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN

Query:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME
                                        VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME
Subjt:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME

Query:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL
        DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL
Subjt:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL

XP_022931725.1 protein MIS12 homolog isoform X2 [Cucurbita moschata]

2.0e-109

100

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Query:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
        MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
Subjt:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN

Query:  VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQ
        VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQ
Subjt:  VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQ

Query:  EFLAYLNL
        EFLAYLNL
Subjt:  EFLAYLNL

XP_023515499.1 protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]

1.1e-86

73.53

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Query:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
        MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDL +GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSN
Subjt:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN

Query:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME
                                        VRKENV LNQE QALER+TASSNSQ N FNEALQLYEQ+SVNEMFQEMMGTAS+LRA+IGKLK+R+ME
Subjt:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME

Query:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL
        +SKLT V+K+HTNGDISHHHKGFSNAKLDDIQEFLA L
Subjt:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL

XP_023530578.1 protein MIS12 homolog [Cucurbita pepo subsp. pepo]

1.6e-95

80.42

Show/hide

Query:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
        ME SK +VVFDSLNLDP LFINEALNVVDDLVGDA DFYQSQAS ALK+EGSDRSQDLIMGISRVRTL QSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
Subjt:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN

Query:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME
                                        VRKENVELN EF  LERKTASSNSQANYFNEALQLYEQSSVNEMF+EMMGTASDLRA+IGKLKRRKME
Subjt:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME

Query:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL
        DSKLTNVDKVHTNGDISHHHKGFSNAKLDDI E LAYLNL
Subjt:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL

TrEMBL top hits

e value

%identity

Alignment

A0A6J1E0A1 protein MIS12 homolog

5.6e-86

72.27

Show/hide

Query:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
        MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSN
Subjt:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN

Query:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME
                                        VRKEN+ LNQE QALER+ ASSNSQ N FNEALQLYE++SVNEMFQEMMGTAS+LRA+IGKLK+R+ME
Subjt:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME

Query:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL
        +SKLT V+K+HTNGD SHHHKGFSNAKLDDIQEFLA L
Subjt:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL

A0A6J1EZI4 protein MIS12 homolog isoform X1

9.3e-105

86.67

Show/hide

Query:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
        MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
Subjt:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN

Query:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME
                                        VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME
Subjt:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME

Query:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL
        DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL
Subjt:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL

A0A6J1F096 protein MIS12 homolog isoform X2

9.6e-110

100

Show/hide

Query:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
        MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
Subjt:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN

Query:  VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQ
        VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQ
Subjt:  VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQ

Query:  EFLAYLNL
        EFLAYLNL
Subjt:  EFLAYLNL

A0A6J1JD43 protein MIS12 homolog isoform X1

2.8e-85

72.8

Show/hide

Query:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
        MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSN
Subjt:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN

Query:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQ-EMMGTASDLRAEIGKLKRRKM
                                        VRKEN+ LNQE QALER+TASSNSQ N FNEALQLYEQ+SVNEMFQ EMMGTAS+LRA+IGKLK+R+M
Subjt:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQ-EMMGTASDLRAEIGKLKRRKM

Query:  EDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL
        E+SKLT V+K+HTN DISHHHKGFSNAKLDDIQEFLA L
Subjt:  EDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL

A0A6J1JME3 protein MIS12 homolog isoform X2

1.1e-86

73.11

Show/hide

Query:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN
        MEGSK + VFDSLNL+PQLFINEALN+VDDLV DA DFYQ+QAS +LK+EGSDRSQDLI+GISRVRT  QSGLDKRLAMWEKYCLNHCFSVPE FSLPSN
Subjt:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSN

Query:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME
                                        VRKEN+ LNQE QALER+TASSNSQ N FNEALQLYEQ+SVNEMFQEMMGTAS+LRA+IGKLK+R+ME
Subjt:  --------------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKME

Query:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL
        +SKLT V+K+HTN DISHHHKGFSNAKLDDIQEFLA L
Subjt:  DSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYL

SwissProt top hits

e value

%identity

Alignment

Q2V0Z5 Protein MIS12 homolog

5.9e-40

42.74

Show/hide

Query:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSD--RSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLP
        MEGSKS+ VFDS+NL+PQ+FINEA+N V+D V  A DFY   AS +LK +GSD  +SQ L  GI+RVR L  S +D RL +WE Y L  CF+VP+ F LP
Subjt:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSD--RSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLP

Query:  SN-----------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMED
         +                             V K +VEL+ E QALER + S        NEAL+LY++SS++E+F+EM   AS+LRA + +LK R+M+ 
Subjt:  SN-----------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMED

Query:  SKLTNVDKVHTNGDISHHHKGFS----NAKLDDIQEFLAYL
        S+   V +      + +H K FS    + KL+D+++F A L
Subjt:  SKLTNVDKVHTNGDISHHHKGFS----NAKLDDIQEFLAYL

Arabidopsis top hits

e value

%identity

Alignment

AT5G35520.1 minichromosome instability 12 (mis12)-like

4.2e-41

42.74

Show/hide

Query:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSD--RSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLP
        MEGSKS+ VFDS+NL+PQ+FINEA+N V+D V  A DFY   AS +LK +GSD  +SQ L  GI+RVR L  S +D RL +WE Y L  CF+VP+ F LP
Subjt:  MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSD--RSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLP

Query:  SN-----------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMED
         +                             V K +VEL+ E QALER + S        NEAL+LY++SS++E+F+EM   AS+LRA + +LK R+M+ 
Subjt:  SN-----------------------------VRKENVELNQEFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMED

Query:  SKLTNVDKVHTNGDISHHHKGFS----NAKLDDIQEFLAYL
        S+   V +      + +H K FS    + KL+D+++F A L
Subjt:  SKLTNVDKVHTNGDISHHHKGFS----NAKLDDIQEFLAYL

Sequences

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CDS sequence

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mRNA sequence

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ATGGAAGGAAGTAAGAGCGATGTGGTATTCGATTCGCTGAATCTGGATCCACAGCTCTTCATTAATGAAGCTCTCAATGTTGTTGACGATTTGGTTGGTGACGCCTCCGA
TTTCTATCAATCACAAGCATCAACCGCCTTGAAGAGTGAGGGCTCGGATAGATCCCAGGATCTTATAATGGGAATATCGCGGGTCCGAACTCTCGCCCAATCGGGTCTGG
ATAAGCGATTGGCTATGTGGGAGAAGTACTGTCTCAATCACTGTTTTTCAGTCCCTGAAGATTTTTCTTTACCCTCAAATGTCAGGAAAGAGAATGTTGAATTGAACCAG
GAATTCCAAGCTTTAGAAAGGAAAACTGCTTCAAGTAACTCCCAAGCCAATTATTTCAACGAAGCATTACAGTTATACGAGCAAAGCTCTGTGAATGAGATGTTCCAGGA
AATGATGGGAACTGCATCGGATCTGCGAGCGGAAATAGGAAAGCTGAAGAGAAGGAAGATGGAGGATTCCAAGCTCACTAACGTAGATAAGGTTCATACAAACGGAGACA
TCTCTCACCATCACAAGGGTTTCTCTAATGCTAAGCTGGATGACATTCAAGAATTTTTAGCCTATTTGAACCTCTGAACTTCTTCATTATGACATCAACCTTACTGTATA
TGATGCTTGCTGTGGATCTTGTCACTATGAGTTCTTCCATGTTTTAACTTGATTTAGCTGTGAGATATTCTAATTCAAATTTGTATAAATATATCATTTGGACTGATGAG
TCTCCTTAATAGTCTCCATAGAGAATTTGCAGATGGGCCATGAACAGGCGTTGCCTCCCCAGATAGATCCGTACATATTTTCTCATGAAATTGAATTGCAGTTCATATTA
TTTTGGAACAGCTGGTGTAGATATCAAAAGGAATCATTTCTGAGGCTCTTGAACAAAAGTAAGCCTAAACTACCAACTGCAAGCGTTAGTAAAAGTGGGTTTCTGATGGA
TTCAAAATCAGCAATCCTCCACATCCCCTCGTTGTCAGCTTTGCCCCGATGACTCCTCTCGCTCGAGTACTCCCGGAGAAGATTGCTCAAATTGTCAACTTGGAGGATAA
TTTGACGCTGCGCCCGTGCTATATTTTATACCTCTTCTAGCAGGGGAGATTCCTTGGCCAATTGGGAGGACGACAAAGAATGAGATAGCAGCGATCCAGTTAAGGCACCA
TTACTCAAGCCAGATATGAAAATTGAAGTTGCCCCGGAGCCGTTGTTGCCCGCATCAGCTTGTGAAGACAAGTTCTGCTGGCTAGCAGAAACGTTTCTAGTAGAGATCTT
GGAGTTCAGCTCATCAATACGAGTTGTGAACTCATCCATCCTCTCGCTGAGAGTAGAAATCTGTTCTGAAAGTTGTGTAATGGCTCCCTGAAAGAATTTTGAGATGGATC
AGCAAATTAAAAAGCTGAGGACTTCCTCGACTCATTCAGCAATACTGTTAACCAAGAAACTATATGAATGTTTATCTCTTCTTTGGGTCAAAAGTCTCTACCAACACGTT
CAGCCTCGTACTAATAGAGACGTTAATTTTTATCCCAAAGCTTTTGAATTCTAGCTTCGTTTTTAGCCATCATTGGACGGTTAATTAGATTCATAATCTTCCTACGTTAT
CCTATCTTGGAATATA

Protein sequence

Show/hide protein sequence

MEGSKSDVVFDSLNLDPQLFINEALNVVDDLVGDASDFYQSQASTALKSEGSDRSQDLIMGISRVRTLAQSGLDKRLAMWEKYCLNHCFSVPEDFSLPSNVRKENVELNQ
EFQALERKTASSNSQANYFNEALQLYEQSSVNEMFQEMMGTASDLRAEIGKLKRRKMEDSKLTNVDKVHTNGDISHHHKGFSNAKLDDIQEFLAYLNL

Carg09169 (gene) of Silver-seed gourd (SMH-JMG-627) v2 genome