CuGenDBv2

Gene ID	Carg13399
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DNA-directed RNA polymerase III subunit RPC8
Genome location	Carg_Chr08:2564333..2566551
RNA-Seq Expression	Carg13399
Synteny	Carg13399
Gene Ontology terms	GO:0006384 - transcription initiation from RNA polymerase III promoter (biological process) GO:0005666 - RNA polymerase III complex (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0003899 - DNA-directed 5'-3' RNA polymerase activity (molecular function)
InterPro domains	IPR036898 - RNA polymerase Rpb7-like, N-terminal domain superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6575744.1 DNA-directed RNA polymerase III subunit RPC8, partial [Cucurbita argyrosperma subsp. sororia]

1.8e-14

47.37

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Query:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------
        LDAIKGELEKLFLDK      VVA LGLCISVYDIRSVD GFIFPSDG A YK                 G+V       LR +L               
Subjt:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------

Query:  ------------QPMWVLTDYDSQPYDIYETDE
                    +  WV  DYD QPYDIYETDE
Subjt:  ------------QPMWVLTDYDSQPYDIYETDE

KAG7025609.1 hypothetical protein SDJN02_12106, partial [Cucurbita argyrosperma subsp. argyrosperma]

2.8e-156

100

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Query:  MVSSWSERMISHTGTETQPRILREAAVENFLQWDDIKEPQCYYSNKHETYCCIFVSGSNRTYMLFDIALNLVSLTLPAKTSLLSGAATASASSSLPLGFA
        MVSSWSERMISHTGTETQPRILREAAVENFLQWDDIKEPQCYYSNKHETYCCIFVSGSNRTYMLFDIALNLVSLTLPAKTSLLSGAATASASSSLPLGFA
Subjt:  MVSSWSERMISHTGTETQPRILREAAVENFLQWDDIKEPQCYYSNKHETYCCIFVSGSNRTYMLFDIALNLVSLTLPAKTSLLSGAATASASSSLPLGFA

Query:  NQCIEPQLSIGEGNECQRGHWPLRSGRLDPTSVGEGNETFFTRFWIVTLEECGLVGTNRLDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRG
        NQCIEPQLSIGEGNECQRGHWPLRSGRLDPTSVGEGNETFFTRFWIVTLEECGLVGTNRLDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRG
Subjt:  NQCIEPQLSIGEGNECQRGHWPLRSGRLDPTSVGEGNETFFTRFWIVTLEECGLVGTNRLDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRG

Query:  FIFPSDGVAMYKGEVSTPVQGMLRSTLQPMWVLTDYDSQPYDIYETDEAAIDCDGVGTYFMMEWLGRCRC
        FIFPSDGVAMYKGEVSTPVQGMLRSTLQPMWVLTDYDSQPYDIYETDEAAIDCDGVGTYFMMEWLGRCRC
Subjt:  FIFPSDGVAMYKGEVSTPVQGMLRSTLQPMWVLTDYDSQPYDIYETDEAAIDCDGVGTYFMMEWLGRCRC

XP_022152296.1 DNA-directed RNA polymerase III subunit RPC8 isoform X2 [Momordica charantia]

3.7e-15

48.12

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Query:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------
        LDAIKGELEKLFLDK      VVANLGLCISVYDIRSVD GFIFPSDG A YK                 G+V       LR +L               
Subjt:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------

Query:  ------------QPMWVLTDYDSQPYDIYETDE
                    +  WV  DYD QPYDIYETDE
Subjt:  ------------QPMWVLTDYDSQPYDIYETDE

XP_022991804.1 DNA-directed RNA polymerase III subunit RPC8 [Cucurbita maxima]

3.7e-15

48.12

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Query:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------
        LDAIKGELEKLFLDK      VVANLGLCISVYDIRSVD GFIFPSDG A YK                 G+V       LR +L               
Subjt:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------

Query:  ------------QPMWVLTDYDSQPYDIYETDE
                    +  WV  DYD QPYDIYETDE
Subjt:  ------------QPMWVLTDYDSQPYDIYETDE

XP_023547503.1 DNA-directed RNA polymerase III subunit RPC8 [Cucurbita pepo subsp. pepo]

3.7e-15

48.12

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Query:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------
        LDAIKGELEKLFLDK      VVANLGLCISVYDIRSVD GFIFPSDG A YK                 G+V       LR +L               
Subjt:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------

Query:  ------------QPMWVLTDYDSQPYDIYETDE
                    +  WV  DYD QPYDIYETDE
Subjt:  ------------QPMWVLTDYDSQPYDIYETDE

TrEMBL top hits

e value

%identity

Alignment

A0A4D6MSE4 DNA-directed RNA polymerase III subunit RPC8

7.5e-14

46.3

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Query:  DAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYKGE-----------VSTPVQGMLRSTLQPMWVLTDYDSQPYDIYETDEA
        +AI  ELEKLFLDK      V+ANLGLCISVYDIRS+D GFIFP DG   Y G+           V +  +    S  + +W   D++ Q Y I ETD  
Subjt:  DAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYKGE-----------VSTPVQGMLRSTLQPMWVLTDYDSQPYDIYETDEA

Query:  AIDCDGVG
        ++D DG+G
Subjt:  AIDCDGVG

A0A5A7UWI5 DNA-directed RNA polymerase III subunit RPC8 isoform X1

1.8e-12

81.13

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Query:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK
        LDAIKGELEKLF+DK      VVANLGLCISVYDIRSVD GFIFPSDG A YK
Subjt:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK

A0A6J1DFX3 DNA-directed RNA polymerase III subunit RPC8 isoform X2

1.8e-15

48.12

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Query:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------
        LDAIKGELEKLFLDK      VVANLGLCISVYDIRSVD GFIFPSDG A YK                 G+V       LR +L               
Subjt:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------

Query:  ------------QPMWVLTDYDSQPYDIYETDE
                    +  WV  DYD QPYDIYETDE
Subjt:  ------------QPMWVLTDYDSQPYDIYETDE

A0A6J1GPX5 DNA-directed RNA polymerase III subunit RPC8

8.8e-15

47.37

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Query:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------
        LDAIKGELEKLFLDK      VVA LGLCISVYDIRSVD GFIFPSDG A YK                 G+V       LR +L               
Subjt:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------

Query:  ------------QPMWVLTDYDSQPYDIYETDE
                    +  WV  DYD QPYDIYETDE
Subjt:  ------------QPMWVLTDYDSQPYDIYETDE

A0A6J1JVU8 DNA-directed RNA polymerase III subunit RPC8

1.8e-15

48.12

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Query:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------
        LDAIKGELEKLFLDK      VVANLGLCISVYDIRSVD GFIFPSDG A YK                 G+V       LR +L               
Subjt:  LDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK-----------------GEVSTPVQGMLRSTL---------------

Query:  ------------QPMWVLTDYDSQPYDIYETDE
                    +  WV  DYD QPYDIYETDE
Subjt:  ------------QPMWVLTDYDSQPYDIYETDE

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G06790.1 RNA polymerase Rpb7 N-terminal domain-containing protein

6.5e-10

55.77

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Query:  DAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK
        DAIK  L+ +FLDK      V+A+LGLC+S+YDI+SV+ GF+ P DG A YK
Subjt:  DAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK

AT1G06790.2 RNA polymerase Rpb7 N-terminal domain-containing protein

6.5e-10

55.77

Show/hide

Query:  DAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK
        DAIK  L+ +FLDK      V+A+LGLC+S+YDI+SV+ GF+ P DG A YK
Subjt:  DAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYK

Sequences

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CDS sequence

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ATGGTCAGTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACAGCCTAGAATCCTACGGGAGGCAGCAGTGGAGAATTTTCTGCAATGGGATGATATTAA
GGAACCTCAGTGCTATTACTCAAACAAGCATGAAACGTACTGTTGCATATTCGTCAGTGGGTCAAATCGGACTTATATGTTATTCGATATCGCCTTGAATCTTGTTTCAC
TCACTCTTCCCGCCAAAACCTCGCTTTTGTCCGGTGCCGCCACCGCCTCCGCCTCCTCCTCCCTCCCGCTCGGCTTTGCAAATCAGTGCATCGAGCCTCAACTATCGATT
GGAGAGGGAAATGAGTGCCAGCGAGGACACTGGCCCCTAAGGAGTGGACGGTTAGATCCTACGTCGGTTGGAGAGGGGAACGAAACATTCTTTACAAGGTTTTGGATCGT
TACACTCGAAGAATGTGGTTTGGTTGGAACTAATCGACTTGATGCCATTAAGGGTGAGCTGGAGAAACTATTCCTGGATAAGATGAACTTTGTCATGAATGTGGTTGCAA
ACTTGGGGCTCTGTATTTCTGTTTATGATATAAGATCGGTTGATAGGGGCTTTATCTTTCCCAGTGATGGCGTTGCAATGTACAAGGGAGAGGTTTCCACACCCGTACAA
GGAATGCTTCGTTCCACTCTCCAACCGATGTGGGTTCTCACAGACTATGACAGTCAACCATATGACATATATGAAACGGATGAGGCAGCAATTGATTGTGATGGAGTGGG
GACCTATTTCATGATGGAATGGCTCGGGAGATGCCGATGCTGA

mRNA sequence

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Protein sequence

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MVSSWSERMISHTGTETQPRILREAAVENFLQWDDIKEPQCYYSNKHETYCCIFVSGSNRTYMLFDIALNLVSLTLPAKTSLLSGAATASASSSLPLGFANQCIEPQLSI
GEGNECQRGHWPLRSGRLDPTSVGEGNETFFTRFWIVTLEECGLVGTNRLDAIKGELEKLFLDKMNFVMNVVANLGLCISVYDIRSVDRGFIFPSDGVAMYKGEVSTPVQ
GMLRSTLQPMWVLTDYDSQPYDIYETDEAAIDCDGVGTYFMMEWLGRCRC

Carg13399 (gene) of Silver-seed gourd (SMH-JMG-627) v2 genome