CuGenDBv2

Gene ID	Carg13454
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	SpoU_methylase domain-containing protein
Genome location	Carg_Chr08:2921481..2922082
RNA-Seq Expression	Carg13454
Synteny	Carg13454
Gene Ontology terms	GO:0002131 - wobble position cytosine ribose methylation (biological process) GO:0002132 - wobble position uridine ribose methylation (biological process) GO:0003723 - RNA binding (molecular function) GO:0008173 - RNA methyltransferase activity (molecular function)
InterPro domains	IPR001537 - tRNA/rRNA methyltransferase, SpoU type IPR016914 - tRNA (cytidine/uridine-2'-O-)-methyltransferase IPR029026 - tRNA (guanine-N1-)-methyltransferase, N-terminal IPR029028 - Alpha/beta knot methyltransferases

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6593315.1 hypothetical protein SDJN03_12791, partial [Cucurbita argyrosperma subsp. sororia]

1.9e-54

86.26

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Query:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
        YVVVKIHNTWAEFRDYFKKQ                  DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
Subjt:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA

Query:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA
        SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA
Subjt:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA

KAG7025664.1 trmL, partial [Cucurbita argyrosperma subsp. argyrosperma]

6.2e-66

100

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Query:  MVRCFQVLTSCFRYVVVKIHNTWAEFRDYFKKQDFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLN
        MVRCFQVLTSCFRYVVVKIHNTWAEFRDYFKKQDFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLN
Subjt:  MVRCFQVLTSCFRYVVVKIHNTWAEFRDYFKKQDFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLN

Query:  YQQLQLEPEGCTNNNEQSIITEDLFA
        YQQLQLEPEGCTNNNEQSIITEDLFA
Subjt:  YQQLQLEPEGCTNNNEQSIITEDLFA

XP_022959577.1 uncharacterized protein LOC111460609 [Cucurbita moschata]

1.9e-54

86.26

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Query:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
        YVVVKIHNTWAEFRDYFKKQ                  DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
Subjt:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA

Query:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA
        SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA
Subjt:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA

XP_023004870.1 uncharacterized protein LOC111498049 [Cucurbita maxima]

2.7e-53

84.73

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Query:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
        YVVVKIHNTWAEFRDYFKKQ                  DFSYKQGDYLLF SETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
Subjt:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA

Query:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA
        SRQLNYQQLQ+EPEGCTNNNEQSIITEDLFA
Subjt:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA

XP_023514221.1 uncharacterized protein LOC111778552 [Cucurbita pepo subsp. pepo]

1.3e-52

84.85

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Query:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
        YVVVKIHNTWAEFRDYF+KQ                  DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
Subjt:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA

Query:  SRQLNYQQLQLEPEGCTNNNEQS-IITEDLFA
        SRQLNYQQLQLEPEGCTNNNEQS IITEDLFA
Subjt:  SRQLNYQQLQLEPEGCTNNNEQS-IITEDLFA

TrEMBL top hits

e value

%identity

Alignment

A0A0A0K6L9 SpoU_methylase domain-containing protein

2.6e-46

75.57

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Query:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
        YVVVKIHNTWA+F++YFKKQ                  DFSYK+GDYLLFGSET+GLP EALEDCKNQ FGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
Subjt:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA

Query:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA
        SRQLNY+QLQ+  E CT NNEQSIITED+FA
Subjt:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA

A0A5D3CFA4 Putative tRNA (Cytidine(34)-2'-O)-methyltransferase

1.0e-45

74.81

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Query:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
        YVVVKIHNTWA+F++YFKKQ                  DFSYK+GDYLLFGSET+GLP EALEDCKNQ FGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
Subjt:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA

Query:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA
        SRQLNY+QLQ+  E CT N+EQSIITED+FA
Subjt:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA

A0A6J1CVM2 uncharacterized protein LOC111015191

7.7e-46

73.28

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Query:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
        YVV+KIH+TWA+F+DYFKKQ                  +FSY++GDYLLFGSETSGLPAEALEDCKNQ FGGGTLRIPMVETYVRCLNLSVSVGIA+YEA
Subjt:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA

Query:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA
        SRQLNY+QLQ   EGC NN+EQS+ITED+FA
Subjt:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA

A0A6J1H4X6 uncharacterized protein LOC111460609

9.1e-55

86.26

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Query:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
        YVVVKIHNTWAEFRDYFKKQ                  DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
Subjt:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA

Query:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA
        SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA
Subjt:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA

A0A6J1KVT5 uncharacterized protein LOC111498049

1.3e-53

84.73

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Query:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
        YVVVKIHNTWAEFRDYFKKQ                  DFSYKQGDYLLF SETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
Subjt:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA

Query:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA
        SRQLNYQQLQ+EPEGCTNNNEQSIITEDLFA
Subjt:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA

SwissProt top hits

e value

%identity

Alignment

C7BSD3 tRNA (cytidine(34)-2'-O)-methyltransferase

2.3e-07

46.97

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Query:  SYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY
        SY+ GDYL+FG ET GLP+  L++   Q      +RIPM+    R +NLS SV + ++EA RQL Y
Subjt:  SYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY

P0AGJ7 tRNA (cytidine(34)-2'-O)-methyltransferase

1.0e-07

48.48

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Query:  SYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY
        SY+ GDYL+FG ET GLPA  L+    +      +RIPMV    R +NLS +V + +YEA RQL Y
Subjt:  SYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY

P0AGJ8 tRNA (cytidine(34)-2'-O)-methyltransferase

1.0e-07

48.48

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Query:  SYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY
        SY+ GDYL+FG ET GLPA  L+    +      +RIPMV    R +NLS +V + +YEA RQL Y
Subjt:  SYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY

P74516 Putative tRNA (cytidine(34)-2'-O)-methyltransferase

2.3e-07

36.27

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Query:  YVVVKIHNTWAEFRDYFKKQ----------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR
        YV +  HN+  EF  +++ Q                D++Y+  D+L+ GSET GLP + + D   Q      L IPM  + VR LNL+ S  IA++EA R
Subjt:  YVVVKIHNTWAEFRDYFKKQ----------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASR

Query:  QL
        QL
Subjt:  QL

Q74Y93 tRNA (cytidine(34)-2'-O)-methyltransferase

5.2e-07

48.48

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Query:  SYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY
        SY+  DYLLFG ET GLPA  L+    Q      +RIPM +   R +NLS +V + +YEA RQL Y
Subjt:  SYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNY

Arabidopsis top hits

e value

%identity

Alignment

AT5G26880.1 AGAMOUS-like 26

3.0e-26

60.44

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Query:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSV
        +VVVK H++WAEF++YF+ Q                  DFSY+ GDYLLFGSETSGLP EAL DC ++ +GGGTLRIPMVETYVRCLNLS+
Subjt:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSV

AT5G26880.2 AGAMOUS-like 26

3.9e-42

61.83

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Query:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA
        +VVVK H++WAEF++YF+ Q                  DFSY+ GDYLLFGSETSGLP EAL DC ++ +GGGTLRIPMVETYVRCLNLSVSVGIA+YEA
Subjt:  YVVVKIHNTWAEFRDYFKKQ------------------DFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEA

Query:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA
        SRQLNY+Q++  P+GC  N E+ ++TED+FA
Subjt:  SRQLNYQQLQLEPEGCTNNNEQSIITEDLFA

Sequences

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CDS sequence

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ATGGTTCGATGTTTTCAAGTTTTAACATCATGTTTTAGATACGTCGTCGTTAAAATTCATAACACGTGGGCAGAGTTCCGAGACTACTTCAAAAAACAGGATTTTTCGTA
CAAGCAGGGCGATTACTTGTTGTTCGGGTCGGAAACTAGTGGGTTACCAGCTGAAGCTCTTGAGGATTGCAAGAACCAGGCATTTGGTGGGGGGACCTTAAGAATTCCAA
TGGTTGAAACTTATGTTAGATGCCTAAATCTTTCTGTAAGTGTTGGCATTGCTATATATGAAGCATCACGACAACTAAATTACCAGCAACTTCAGCTTGAACCAGAGGGT
TGTACCAACAACAATGAACAGTCTATCATTACAGAGGATCTTTTTGCTTGA

mRNA sequence

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Protein sequence

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MVRCFQVLTSCFRYVVVKIHNTWAEFRDYFKKQDFSYKQGDYLLFGSETSGLPAEALEDCKNQAFGGGTLRIPMVETYVRCLNLSVSVGIAIYEASRQLNYQQLQLEPEG
CTNNNEQSIITEDLFA

Carg13454 (gene) of Silver-seed gourd (SMH-JMG-627) v2 genome