CuGenDBv2

Gene ID	Carg25402
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DNA-directed RNA polymerase subunit beta'
Genome location	Carg_Chr18:3679987..3681143
RNA-Seq Expression	Carg25402
Synteny	Carg25402
Gene Ontology terms	GO:0005488 - binding (molecular function) GO:0016740 - transferase activity (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

CAB4289788.1 unnamed protein product [Prunus armeniaca]

4.5e-17

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT
        EKLVQE V  LLDN I GQPMRD HNKVYKSFSDV+EG                          SLSLH+CGLP EI   L+                QT
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT

Query:  SELLRVKFGKKNPLYGKYLRKV
         ELLRVKFGKK+ LYGKY  K+
Subjt:  SELLRVKFGKKNPLYGKYLRKV

KAG7012587.1 DNA-directed RNA polymerase subunit beta', partial [Cucurbita argyrosperma subsp. argyrosperma]

2.4e-74

100

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Query:  MCNLGSPSTEAVRSIPIPWNIRHHEHYTVDGMLHLLNLGTGEKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGSSLSLHQCGLPSEIKEVLYGH
        MCNLGSPSTEAVRSIPIPWNIRHHEHYTVDGMLHLLNLGTGEKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGSSLSLHQCGLPSEIKEVLYGH
Subjt:  MCNLGSPSTEAVRSIPIPWNIRHHEHYTVDGMLHLLNLGTGEKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGSSLSLHQCGLPSEIKEVLYGH

Query:  PMLLNNRAFPDILLQTSELLRVKFGKKNPLYGKYLRKVL
        PMLLNNRAFPDILLQTSELLRVKFGKKNPLYGKYLRKVL
Subjt:  PMLLNNRAFPDILLQTSELLRVKFGKKNPLYGKYLRKVL

VVW90806.1 unnamed protein product, partial [Nymphaea colorata]

8.0e-14

45.45

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Query:  KLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQTS
        KLVQE V  LLDN I GQPMRD HNKVYKSFSDV+EG                          SLSLHQCGLP EI   L+     +        L    
Subjt:  KLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQTS

Query:  ELLRVKFGKKNPLYGKYLRKV
         L + K  +KNPLYGKY +K+
Subjt:  ELLRVKFGKKNPLYGKYLRKV

VVW91431.1 unnamed protein product, partial [Nymphaea colorata]

2.1e-14

45.9

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT
        EKLVQE V  LLDN I GQPMRD HNKVYKSFSDV+EG                          SLSLHQCGLP EI   L+     +        L   
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT

Query:  SELLRVKFGKKNPLYGKYLRKV
          L + K  +KNPLYGKY +K+
Subjt:  SELLRVKFGKKNPLYGKYLRKV

XP_040955121.1 DNA-directed RNA polymerase subunit beta'-like [Gossypium hirsutum]

1.6e-14

45.6

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPM---LLNNRAFPDIL
        EKLVQE V   LDN I GQPMRD HNKVYKSFSDV+EG                          SLSLH+CGLP EI   L+   +   L+     P+I 
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPM---LLNNRAFPDIL

Query:  LQTSELLRVKFGKKNPLYGKYLRKV
             + +VKFGKK  LYGKY +K+
Subjt:  LQTSELLRVKFGKKNPLYGKYLRKV

TrEMBL top hits

e value

%identity

Alignment

A0A452YM89 DNA-directed RNA polymerase subunit

6.6e-14

44.8

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPM---LLNNRAFPDIL
        EKLVQEVV  LLD+   GQP RD HNKVYKS SDV+EG                          SLSLHQCGLP EI   L+   +   L+  RA  ++ 
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPM---LLNNRAFPDIL

Query:  LQTSELLRVKFGKKNPLYGKYLRKV
             + + K GKKNPLYGKY +K+
Subjt:  LQTSELLRVKFGKKNPLYGKYLRKV

A0A5K1HQG7 DNA-directed RNA polymerase subunit (Fragment)

1.0e-14

45.9

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT
        EKLVQE V  LLDN I GQPMRD HNKVYKSFSDV+EG                          SLSLHQCGLP EI   L+     +        L   
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT

Query:  SELLRVKFGKKNPLYGKYLRKV
          L + K  +KNPLYGKY +K+
Subjt:  SELLRVKFGKKNPLYGKYLRKV

A0A5K1HS53 DNA-directed RNA polymerase subunit (Fragment)

3.9e-14

45.45

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Query:  KLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQTS
        KLVQE V  LLDN I GQPMRD HNKVYKSFSDV+EG                          SLSLHQCGLP EI   L+     +        L    
Subjt:  KLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQTS

Query:  ELLRVKFGKKNPLYGKYLRKV
         L + K  +KNPLYGKY +K+
Subjt:  ELLRVKFGKKNPLYGKYLRKV

A0A6J5VV95 DNA-directed RNA polymerase subunit

2.2e-17

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT
        EKLVQE V  LLDN I GQPMRD HNKVYKSFSDV+EG                          SLSLH+CGLP EI   L+                QT
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT

Query:  SELLRVKFGKKNPLYGKYLRKV
         ELLRVKFGKK+ LYGKY  K+
Subjt:  SELLRVKFGKKNPLYGKYLRKV

W1P4N9 DNA-directed RNA polymerase subunit (Fragment)

1.1e-13

52.58

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGSSLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQTSELLRVKFGKKNPLYGKYLRKV
        EKLVQE V  LLDN I GQPMRD HNKVYKSFSDV+EG              +E L G  +  + R+         ELLRVKF KK+ LYGKY RK+
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGSSLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQTSELLRVKFGKKNPLYGKYLRKV

SwissProt top hits

e value

%identity

Alignment

A0ZZ26 DNA-directed RNA polymerase subunit beta'

3.1e-13

41.27

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPM---LLNNRAFPDIL
        EKLVQE V  LLDN I GQPMRD HNKVYKSFSDV+EG                          SLSLH+CGLP EI   L+   +   L+     P+I 
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPM---LLNNRAFPDIL

Query:  LQTSELLRVKFGKKNPLYGKYLRKVL
        +  S     K  +K P+  + L++V+
Subjt:  LQTSELLRVKFGKKNPLYGKYLRKVL

A6MMJ8 DNA-directed RNA polymerase subunit beta'

3.1e-13

39.84

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT
        EKLVQE V  LLDN I GQPM+D HNKVYKSFSDV+EG                          SLSLHQCGLP EI   L+     L +      +   
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT

Query:  SELLRVKFGKKNPLYGKYLRKVL
          + + K  +K P+  + L++V+
Subjt:  SELLRVKFGKKNPLYGKYLRKVL

A8W3B5 DNA-directed RNA polymerase subunit beta'

1.1e-13

40.65

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT
        EKLVQE V  LLDN I GQPMRD HNKVYKSFSD++EG                          SLSLHQCGLP EI   L+  P ++ +      L   
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT

Query:  SELLRVKFGKKNPLYGKYLRKVL
          + + K  +K P+  + L+ V+
Subjt:  SELLRVKFGKKNPLYGKYLRKVL

Q589B9 DNA-directed RNA polymerase subunit beta'

2.4e-13

40.65

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT
        EKLVQE V  LLDN I GQPMRD HNKVYKSFSDV+EG                          SLSLH+CGLP EI   L+     +        L   
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT

Query:  SELLRVKFGKKNPLYGKYLRKVL
          + + K  +K P+  K L++V+
Subjt:  SELLRVKFGKKNPLYGKYLRKVL

Q6EW57 DNA-directed RNA polymerase subunit beta'

1.4e-13

41.46

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT
        EKLVQE V  LLDN I GQPMRD HNKVYKSFSDV+EG                          SLSLHQCGLP EI   L+     +        L   
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLYGHPMLLNNRAFPDILLQT

Query:  SELLRVKFGKKNPLYGKYLRKVL
          L + K  +K P+  + L++V+
Subjt:  SELLRVKFGKKNPLYGKYLRKVL

Arabidopsis top hits

e value

%identity

Alignment

ATCG00180.1 DNA-directed RNA polymerase family protein

8.5e-14

52.44

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Query:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLY
        EKLVQE V  LLDN I GQPMRD HNKVYKSFSDV+EG                          SLSLH+CGLP EI   L+
Subjt:  EKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGS-------------------------SLSLHQCGLPSEIKEVLY

Sequences

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CDS sequence

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ATGTGTAACCTGGGTAGTCCAAGTACTGAAGCAGTGAGAAGTATACCAATCCCGTGGAACATACGTCATCACGAACATTACACTGTCGATGGAATGCTTCACCTGCTAAA
CCTAGGAACTGGAGAGAAATTGGTACAAGAAGTCGTGGGTATACTTCTGGATAATGAAATTATTGGACAACCAATGAGGGATGACCATAATAAGGTTTACAAGTCGTTCT
CCGATGTAGTTGAAGGTTCCTCGCTTTCATTACATCAATGTGGATTACCTAGCGAAATTAAGGAAGTTTTGTATGGACATCCTATGTTACTAAACAATAGAGCTTTTCCA
GATATTTTGCTTCAAACATCGGAGTTGTTAAGAGTAAAATTCGGGAAGAAGAACCCCTTATATGGAAAATACTTAAGGAAAGTTTTGTAG

mRNA sequence

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Protein sequence

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MCNLGSPSTEAVRSIPIPWNIRHHEHYTVDGMLHLLNLGTGEKLVQEVVGILLDNEIIGQPMRDDHNKVYKSFSDVVEGSSLSLHQCGLPSEIKEVLYGHPMLLNNRAFP
DILLQTSELLRVKFGKKNPLYGKYLRKVL

Carg25402 (gene) of Silver-seed gourd (SMH-JMG-627) v2 genome