CuGenDBv2

Gene ID	Carg27332
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	THO complex subunit 5B
Genome location	Carg_Chr09:999685..1000833
RNA-Seq Expression	Carg27332
Synteny	Carg27332
Gene Ontology terms	GO:0006406 - mRNA export from nucleus (biological process) GO:0032786 - positive regulation of DNA-templated transcription, elongation (biological process) GO:0000445 - THO complex part of transcription export complex (cellular component) GO:0003729 - mRNA binding (molecular function)
InterPro domains	IPR019163 - THO complex, subunit 5

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6591379.1 THO complex subunit 5B, partial [Cucurbita argyrosperma subsp. sororia]

9.0e-80

100

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Query:  NIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASLFSE
        NIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASLFSE
Subjt:  NIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASLFSE

Query:  RRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
        RRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
Subjt:  RRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

KAG7024258.1 THO complex subunit 5B, partial [Cucurbita argyrosperma subsp. argyrosperma]

2.1e-81

100

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Query:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL
        MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL
Subjt:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL

Query:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
        FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
Subjt:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

XP_004141378.1 THO complex subunit 5B [Cucumis sativus]

2.5e-61

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Query:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL
        + RN + P +NLKLQ +IK+SMEYPLRPP+FTLNLY+M SEEN +E D+SDWYNE + MEAEVNL  L+MLPLDQENYI SHQICCLAMLFNYCI EASL
Subjt:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL

Query:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
        FSERR  SSV DIGL KPVSGSLHAR  RGRDRRKMISWKDIE +PGYPC
Subjt:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

XP_022982205.1 THO complex subunit 5B [Cucurbita maxima]

1.4e-59

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Query:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL
        M RN DSP +NLKLQ +IK+SMEYPLRPP FTLNLY++ SEEN DESD+SDWYNE + MEAEVNL  L MLPLDQENYI SHQICCL MLFNYCI+EAS 
Subjt:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL

Query:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
         SER+  S V D+GL KPVSGS+HAR  RGRDRRKMISWKDIE +PGYPC
Subjt:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

XP_023524487.1 THO complex subunit 5B [Cucurbita pepo subsp. pepo]

1.4e-59

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Query:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL
        M RN DSP +NLKLQ +IK+SMEYPLRPP FTLNLY++ SEEN DESD+SDWYNE + MEAEVNL  L+MLPLDQENYI SHQICCL MLFNYCI EAS 
Subjt:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL

Query:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
         SER+  S V D+GL KPVSGS+HAR  RGRDRRKMISWKDIE +PGYPC
Subjt:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KZS2 Uncharacterized protein

1.2e-61

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Query:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL
        + RN + P +NLKLQ +IK+SMEYPLRPP+FTLNLY+M SEEN +E D+SDWYNE + MEAEVNL  L+MLPLDQENYI SHQICCLAMLFNYCI EASL
Subjt:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL

Query:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
        FSERR  SSV DIGL KPVSGSLHAR  RGRDRRKMISWKDIE +PGYPC
Subjt:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

A0A5A7VBI1 THO complex subunit 5B

1.5e-59

76.67

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Query:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL
        + RN + P +NLKLQ +IK+SMEYPLRPP+FTLNLY+M SEEN +ESD+SDWYNE + MEAEVNL  L+MLPLDQENYI SHQI CLAMLFNYCI EASL
Subjt:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL

Query:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
         SERR  SSV D+GL KPVSGSLHAR  RGRDRRKMISWKDIE +PGYPC
Subjt:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

A0A5D3D9N3 THO complex subunit 5B

1.5e-59

76.67

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Query:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL
        + RN + P +NLKLQ +IK+SMEYPLRPP+FTLNLY+M SEEN +ESD+SDWYNE + MEAEVNL  L+MLPLDQENYI SHQI CLAMLFNYCI EASL
Subjt:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL

Query:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
         SERR  SSV D+GL KPVSGSLHAR  RGRDRRKMISWKDIE +PGYPC
Subjt:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

A0A6J1FJR5 THO complex subunit 5B

1.1e-59

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Query:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL
        M RN DSP +NLKLQ +IK+SMEYPLR P FTLNLY++ SEEN DESD+SDWYNE + MEAEVNL  L+MLPLDQENYI SHQICCL MLFNYCI EAS 
Subjt:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL

Query:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
         SER+  SSV D+GL KPVSGS+HAR  RGRDRRKMISWKDIE +PGYPC
Subjt:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

A0A6J1J206 THO complex subunit 5B

6.5e-60

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Query:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL
        M RN DSP +NLKLQ +IK+SMEYPLRPP FTLNLY++ SEEN DESD+SDWYNE + MEAEVNL  L MLPLDQENYI SHQICCL MLFNYCI+EAS 
Subjt:  MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASL

Query:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
         SER+  S V D+GL KPVSGS+HAR  RGRDRRKMISWKDIE +PGYPC
Subjt:  FSERRNCSSVTDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

SwissProt top hits

e value

%identity

Alignment

F4K4J0 THO complex subunit 5B

8.9e-38

55.07

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Query:  KLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASLFSERRNCSSVTD
        KL+  +++SMEYPLRPPLF+L+L++  S  N + ++ SD YNE + MEAEVNL  L+++P DQENY+ SHQI CLAMLF+Y +D+ S  S+R   ++V D
Subjt:  KLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASLFSERRNCSSVTD

Query:  IGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
        +GL KPV G L  R  RGRD RKMISWK    + GYPC
Subjt:  IGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

Arabidopsis top hits

e value

%identity

Alignment

AT5G42920.1 THO complex, subunit 5

6.3e-39

55.07

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Query:  KLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASLFSERRNCSSVTD
        KL+  +++SMEYPLRPPLF+L+L++  S  N + ++ SD YNE + MEAEVNL  L+++P DQENY+ SHQI CLAMLF+Y +D+ S  S+R   ++V D
Subjt:  KLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASLFSERRNCSSVTD

Query:  IGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
        +GL KPV G L  R  RGRD RKMISWK    + GYPC
Subjt:  IGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

AT5G42920.2 THO complex, subunit 5

6.3e-39

55.07

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Query:  KLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASLFSERRNCSSVTD
        KL+  +++SMEYPLRPPLF+L+L++  S  N + ++ SD YNE + MEAEVNL  L+++P DQENY+ SHQI CLAMLF+Y +D+ S  S+R   ++V D
Subjt:  KLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASLFSERRNCSSVTD

Query:  IGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC
        +GL KPV G L  R  RGRD RKMISWK    + GYPC
Subjt:  IGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

Sequences

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CDS sequence

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mRNA sequence

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ATGGCCAGGAACATTGATTCACCTGTCGAAAATCTTAAATTGCAAGACGAGATTAAAGTCAGCATGGAGTACCCTCTTAGGCCTCCTCTCTTCACTTTGAATCTTTACTC
GATGAAGTCTGAAGAAAACCATGATGAGAGTGACAACTCTGATTGGTATAATGAACGTCAGATCATGGAAGCTGAGGTGAATCTTCGTAAACTTGAGATGCTGCCTTTGG
ATCAAGAGAACTATATATCGTCTCATCAAATTTGTTGTCTTGCTATGTTGTTTAACTACTGCATCGACGAGGCATCCCTGTTCTCTGAAAGGAGAAATTGTAGTTCTGTC
ACTGACATTGGATTATACAAACCTGTTAGTGGTAGTTTGCATGCCAGAGTACTTAGAGGAAGGGATCGTAGGAAGATGATATCCTGGAAAGATATTGAAGGCTCTCCCGG
CTATCCTTGCTAAGAACTTTTAGGATCTTAAAAATATTACTTCAGCACCTCCTCCTGCTGCTGCTGCTGATGGTGGAACAGTTCTAGAAAATCTGCCTGCCTGGAAAATA
TGAAGAATGTGATAATTTCCTTCTTAGCCAATTTCTCCAGTATTGAAATATTGATTGATGTAGTGGCCATGGATTGCTACATGCTTGAACCCGATTCTCAGCTATAGAAG
CTTGAATGTTAAGCCAAACTGATCACTTTGTTCTCGTGCATTTGTGTATATAATGAGAGTTCGTTTTCCTTAAATCCCTTTTCTTTGTAATCGAGTTGGGTAATTGAGAG
AATTATTACTATATAGCACTGAGGCGTAACTTTACATGTCAAAA

Protein sequence

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MARNIDSPVENLKLQDEIKVSMEYPLRPPLFTLNLYSMKSEENHDESDNSDWYNERQIMEAEVNLRKLEMLPLDQENYISSHQICCLAMLFNYCIDEASLFSERRNCSSV
TDIGLYKPVSGSLHARVLRGRDRRKMISWKDIEGSPGYPC

Carg27332 (gene) of Silver-seed gourd (SMH-JMG-627) v2 genome