CuGenDBv2

Gene ID	Chy2G046940
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	protein MIS12 homolog
Genome location	chrH02:27953341..27955884
RNA-Seq Expression	Chy2G046940
Synteny	Chy2G046940
Gene Ontology terms	GO:0000070 - mitotic sister chromatid segregation (biological process) GO:0034501 - protein localization to kinetochore (biological process) GO:0051301 - cell division (biological process) GO:0051382 - kinetochore assembly (biological process) GO:0000818 - nuclear MIS12/MIND complex (cellular component)
InterPro domains	IPR008685 - Centromere protein Mis12

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAA0058004.1 Centromere protein Mis12 [Cucumis melo var. makuwa]

4.02e-153

94.21

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Query:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE+VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT
           DESPGVTSI+HD DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+TASELRAKIGK+KK RT
Subjt:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT

Query:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        EE KLA VEK+HTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Subjt:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

KAA0058032.1 Centromere protein Mis12 [Cucumis melo var. makuwa]

4.69e-152

93.39

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Query:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE+VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTEGSDRSQDLTLGISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT
           DESPGVTSI+HD DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+TASELR+KIGK+KK RT
Subjt:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT

Query:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        EE KLA VEK+HTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Subjt:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

XP_004138204.3 protein MIS12 homolog isoform X1 [Cucumis sativus]

2.01e-155

96.28

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Query:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPS+
Subjt:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT
           DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELR KIGKLKK RT
Subjt:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT

Query:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        EETKLAIVEK+HTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
Subjt:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

XP_023515499.1 protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]

2.49e-136

84.84

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Query:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL+LGIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  LSKDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKS
           DESP VTS+S D  +DVDLDTELD LRNKLSEVRKEN+VLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MFQEM+ TASELRAKIGKLKK 
Subjt:  LSKDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKS

Query:  RTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        R EE+KL  VEKIHTNGDISHHHKGFSNAKLDDIQEFL+DLK +
Subjt:  RTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

XP_038879945.1 protein MIS12 homolog [Benincasa hispida]

3.50e-138

86.36

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Query:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGEAVF+SLNLNPQLFINEALN VDDLVDDAF FYQSQASA LKTEGSDRSQDLTLGIS+VRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT
           D+SPGVTSISHD+DV LDTELD LRNKLSEVRKENIVLNQELQALERQTA+SNSQI+HFNEALQLYEQ+SVN+MFQEM+ TASELRAKIGKLKK R 
Subjt:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT

Query:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        EE+KL  VEK+HTNGDISH+H G SNAKLDDI+ FL DLK +
Subjt:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LPL2 Uncharacterized protein

3.1e-120

95.87

Show/hide

Query:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPS+
Subjt:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT
           DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELR KIGKLKK RT
Subjt:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT

Query:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        EETKLAIVEK+HTNGDISHHHKGFSNAKLDDIQEFLS LKKI
Subjt:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

A0A5A7UQ95 Centromere protein Mis12

1.7e-118

93.39

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Query:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE+VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTEGSDRSQDLTLGISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT
           DESPGVTSI+HD DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+TASELR+KIGK+KK RT
Subjt:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT

Query:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        EE KLA VEK+HTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Subjt:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

A0A5A7US57 Centromere protein Mis12

2.7e-119

94.21

Show/hide

Query:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE+VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT
           DESPGVTSI+HD DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+TASELRAKIGK+KK RT
Subjt:  LSKDESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRT

Query:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        EE KLA VEK+HTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Subjt:  EETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

A0A6J1E0A1 protein MIS12 homolog

8.3e-105

84.02

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Query:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL LGIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  LSKDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKS
           DESP VTS+S D  +DVDLDTELD LRNKLSEVRKENIVLNQELQALERQ ASSNSQ++ FNEALQLYE++SVN+MFQEM+ TASELRAKIGKLKK 
Subjt:  LSKDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKS

Query:  RTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        R EE+KL  VEKIHTNGD SHHHKGFSNAKLDDIQEFL+DLK +
Subjt:  RTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

A0A6J1JME3 protein MIS12 homolog isoform X2

3.1e-104

84.02

Show/hide

Query:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL LGIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  LSKDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKS
           DESP VTS+S D  +DVDLDTELD LR  LSEVRKENIVLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MFQEM+ TASELRAKIGKLKK 
Subjt:  LSKDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKS

Query:  RTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        R EE+KL  VEKIHTN DISHHHKGFSNAKLDDIQEFL+DLK +
Subjt:  RTEETKLAIVEKIHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

SwissProt top hits

e value

%identity

Alignment

Q2V0Z5 Protein MIS12 homolog

3.3e-50

46.4

Show/hide

Query:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSD--RSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLP
        MEGSK EAVF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK +GSD  +SQ L+ GI++VR L+   +  RL +WE Y L  CF+VP+GF LP
Subjt:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSD--RSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLP

Query:  SNLSKDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLK
         +   +ES   +S+  D  +D++LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+EM + ASELRA + +LK
Subjt:  SNLSKDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLK

Query:  KSRTEETKLAIVEKIHTNGDISHHHKGFS----NAKLDDIQEFLSDLKKI
          R + ++ A V++      + +H K FS    + KL+D+++F ++L+K+
Subjt:  KSRTEETKLAIVEKIHTNGDISHHHKGFS----NAKLDDIQEFLSDLKKI

Arabidopsis top hits

e value

%identity

Alignment

AT5G35520.1 minichromosome instability 12 (mis12)-like

2.3e-51

46.4

Show/hide

Query:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSD--RSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLP
        MEGSK EAVF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK +GSD  +SQ L+ GI++VR L+   +  RL +WE Y L  CF+VP+GF LP
Subjt:  MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSD--RSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLP

Query:  SNLSKDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLK
         +   +ES   +S+  D  +D++LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+EM + ASELRA + +LK
Subjt:  SNLSKDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLK

Query:  KSRTEETKLAIVEKIHTNGDISHHHKGFS----NAKLDDIQEFLSDLKKI
          R + ++ A V++      + +H K FS    + KL+D+++F ++L+K+
Subjt:  KSRTEETKLAIVEKIHTNGDISHHHKGFS----NAKLDDIQEFLSDLKKI

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGGAAGGAAGTAAAGGCGAAGCGGTGTTCAATTCACTCAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATACTGTCGACGATTTGGTTGATGATGCTTTCGA
TTTCTATCAATCACAAGCATCGGCTGCCTTGAAGACCGAGGGCTCGGATAGATCTCAGGATCTTACCCTGGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGC
ATAAGCGATTGGCCATGTGGGAAAAGTACTGTCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAAATTTGTCTAAGGATGAATCACCTGGTGTCACT
TCAATAAGTCATGACCATGACGTGGATCTGGATACAGAACTTGATCTTTTGAGAAATAAGCTCTCCGAGGTCAGAAAAGAGAACATTGTATTAAACCAAGAACTGCAAGC
TTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGAAATGATCAGAA
CTGCATCGGAGCTTCGTGCGAAAATAGGAAAGCTGAAGAAAAGTAGGACGGAGGAAACCAAGCTCGCTATTGTAGAGAAGATTCATACAAATGGAGACATCTCTCACCAT
CACAAGGGTTTCTCTAATGCTAAGCTGGATGATATTCAAGAATTTTTATCTGATTTGAAGAAGATTTGA

mRNA sequence

Show/hide mRNA sequence

Protein sequence

Show/hide protein sequence

MEGSKGEAVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSNLSKDESPGVT
SISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRAKIGKLKKSRTEETKLAIVEKIHTNGDISHH
HKGFSNAKLDDIQEFLSDLKKI

Chy2G046940 (gene) of Cucumber (hystrix) v1 genome