CuGenDBv2

Gene ID	Cmc02g0045521
Organism	Cucumis melo var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Reverse transcriptase
Genome location	CMiso1.1chr02:9402774..9403199
RNA-Seq Expression	Cmc02g0045521
Synteny	Cmc02g0045521
Gene Ontology terms	GO:0006278 - RNA-dependent DNA biosynthetic process (biological process) GO:0006508 - proteolysis (biological process) GO:0015074 - DNA integration (biological process) GO:0003676 - nucleic acid binding (molecular function) GO:0003964 - RNA-directed DNA polymerase activity (molecular function) GO:0004190 - aspartic-type endopeptidase activity (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	IPR000477 - Reverse transcriptase domain IPR043128 - Reverse transcriptase/Diguanylate cyclase domain IPR043502 - DNA/RNA polymerase superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0035531.1 uncharacterized protein E6C27_scaffold285G001970 [Cucumis melo var. makuwa]

2.0e-60

81.56

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGLTNA  TFCTLMNQV HEYLDKF++VYLDDIVVYS+T+EE ++HLQ++FQKLKENQLYVKREKCSF QERI FLGHVI C RIGM+EGKI  IRDW
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI
        AVPKSV +LRSF GLANYY+RFVEGFSKR SPL ELLKKD+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI

KAA0037220.1 reverse transcriptase [Cucumis melo var. makuwa]

4.4e-60

80.85

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGLTNA  TFCTLMNQV HEYLDKF++VYLDDIVVYS+T+EE ++HLQ++FQKLKENQLYVKREKCSF QERI FLGHVI C RIGM+EGKI  IRDW
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI
        A+PKSV +LRSF GLANYY+RFVEGFSKR SPL ELLKKD+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI

KAA0053390.1 polyprotein [Cucumis melo var. makuwa]

2.6e-60

80.85

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGLTNA  TFCTLMNQV HEYLDKF++VYLDDIVVYS+T+EE ++HLQ++FQKLKENQLYVKREKCSFGQERI FLGHVI C RIGM+EGKI  IRDW
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI
        A+PKSV +LRSF GL NYY+RFVEGFSKR SPL ELLKKD+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI

KAA0067557.1 reverse transcriptase [Cucumis melo var. makuwa]

4.4e-60

80.85

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGLTNA  TFCTLMNQV HEYLDKF++VYLDDIVVYS+T+EE ++HLQ++FQKLKENQLYVKREKCSF QERI FLGHVI C RIGM+EGKI  IRDW
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI
        A+PKSV +LRSF GLANYY+RFVEGFSKR SPL ELLKKD+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI

TYK07955.1 reverse transcriptase [Cucumis melo var. makuwa]

2.6e-60

80.85

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGLTNA  TFCTLMNQV HEYLDKF++VYLDDIVVYS+T+EE ++HLQ++FQKLKENQLYVKREKCSF QERI FLGHVI C RIGM+EGKI  IRDW
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI
        A+PKSV +LRSF GLANYY+RFVEGFSKR SPL ELLKKD+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI

TrEMBL top hits

e value

%identity

Alignment

A0A5A7T1S6 Reverse transcriptase domain-containing protein

9.5e-61

81.56

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGLTNA  TFCTLMNQV HEYLDKF++VYLDDIVVYS+T+EE ++HLQ++FQKLKENQLYVKREKCSF QERI FLGHVI C RIGM+EGKI  IRDW
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI
        AVPKSV +LRSF GLANYY+RFVEGFSKR SPL ELLKKD+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI

A0A5A7UDW8 Polyprotein

1.2e-60

80.85

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGLTNA  TFCTLMNQV HEYLDKF++VYLDDIVVYS+T+EE ++HLQ++FQKLKENQLYVKREKCSFGQERI FLGHVI C RIGM+EGKI  IRDW
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI
        A+PKSV +LRSF GL NYY+RFVEGFSKR SPL ELLKKD+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI

A0A5D3BRZ6 Reverse transcriptase

2.1e-60

80.85

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGLTNA  TFCTLMNQV HEYLDKF++VYLDDIVVYS+T+EE ++HLQ++FQKLKENQLYVKREKCSF QERI FLGHVI C RIGM+EGKI  IRDW
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI
        A+PKSV +LRSF GLANYY+RFVEGFSKR SPL ELLKKD+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI

A0A5D3C4R1 Reverse transcriptase

2.1e-60

80.85

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGLTNA  TFCTLMNQV HEYLDKF++VYLDDIVVYS+T+EE ++HLQ++FQKLKENQLYVKREKCSF QERI FLGHVI C RIGM+EGKI  IRDW
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI
        A+PKSV +LRSF GLANYY+RFVEGFSKR SPL ELLKKD+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI

A0A5D3C8Z6 Reverse transcriptase

1.2e-60

80.85

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGLTNA  TFCTLMNQV HEYLDKF++VYLDDIVVYS+T+EE ++HLQ++FQKLKENQLYVKREKCSF QERI FLGHVI C RIGM+EGKI  IRDW
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI
        A+PKSV +LRSF GLANYY+RFVEGFSKR SPL ELLKKD+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI

SwissProt top hits

e value

%identity

Alignment

P04323 Retrovirus-related Pol polyprotein from transposon 17.6

1.3e-22

36.88

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGL NA  TF   MN +L   L+K  +VYLDDI+V+S++++E    L  +F+KL +  L ++ +KC F ++   FLGHV+    I     KI  I+ +
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI
         +P    ++++F GL  YY++F+  F+    P+ + LKK++
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI

P0CT41 Transposon Tf2-12 polyprotein

3.9e-19

33.33

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MP+G++ A   F   +N +L E  +  ++ Y+DDI+++S +  E   H++ + QKLK   L + + KC F Q ++KF+G+ I+       +  I  +  W
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI
          PK+  +LR F G  NY ++F+   S+   PL  LLKKD+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKDI

P20825 Retrovirus-related Pol polyprotein from transposon 297

6.4e-22

35.97

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGL NA  TF   MN +L   L+K  +VYLDDI+++S+++ E  N +Q +F KL +  L ++ +KC F ++   FLGH++    I     K+  I  +
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKK
         +P    ++R+F GL  YY++F+  ++    P+   LKK
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKK

Q7LHG5 Transposon Ty3-I Gag-Pol polyprotein

5.8e-23

39.85

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGL NA +TF   M     +   +F+ VYLDDI+++S + EE   HL  + ++LK   L VK++KC F  E  +FLG+ I   +I   + K   IRD+
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPL
          PK+V + + F G+ NYY+RF+   SK   P+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPL

Q99315 Transposon Ty3-G Gag-Pol polyprotein

5.8e-23

39.85

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Query:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW
        MPFGL NA +TF   M     +   +F+ VYLDDI+++S + EE   HL  + ++LK   L VK++KC F  E  +FLG+ I   +I   + K   IRD+
Subjt:  MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDW

Query:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPL
          PK+V + + F G+ NYY+RF+   SK   P+
Subjt:  AVPKSVMKLRSFFGLANYYKRFVEGFSKRESPL

Arabidopsis top hits

e value

%identity

Alignment

ATMG00860.1 DNA/RNA polymerases superfamily protein

3.2e-16

40.62

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Query:  NHLQRIFQKLKENQLYVKREKCSFGQERIKFLG--HVINCARIGMKEGKITVIRDWAVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKD
        NHL  + Q  +++Q Y  R+KC+FGQ +I +LG  H+I+   +     K+  +  W  PK+  +LR F GL  YY+RFV+ + K   PL ELLKK+
Subjt:  NHLQRIFQKLKENQLYVKREKCSFGQERIKFLG--HVINCARIGMKEGKITVIRDWAVPKSVMKLRSFFGLANYYKRFVEGFSKRESPLIELLKKD

Sequences

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CDS sequence

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ATGCCATTTGGTCTTACCAACGCCCTTACAACTTTTTGTACACTAATGAACCAGGTCTTGCATGAATATCTTGACAAGTTTATCATGGTCTACCTAGACGATATAGTGGT
CTATAGCTCAACCATAGAGGAACAAAAGAACCACCTTCAAAGGATTTTTCAAAAATTGAAGGAAAACCAACTATATGTTAAGAGAGAAAAATGCTCATTTGGGCAAGAAC
GAATAAAGTTTTTGGGCCATGTGATAAATTGTGCGCGAATTGGGATGAAAGAAGGAAAGATTACTGTGATACGTGATTGGGCAGTACCAAAATCAGTCATGAAATTACGT
TCCTTCTTCGGATTAGCTAATTATTACAAGCGATTCGTGGAAGGATTCTCCAAAAGAGAAAGTCCGCTGATTGAGTTACTGAAAAAGGACATTTAA

mRNA sequence

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Protein sequence

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MPFGLTNALTTFCTLMNQVLHEYLDKFIMVYLDDIVVYSSTIEEQKNHLQRIFQKLKENQLYVKREKCSFGQERIKFLGHVINCARIGMKEGKITVIRDWAVPKSVMKLR
SFFGLANYYKRFVEGFSKRESPLIELLKKDI

Cmc02g0045521 (gene) of Melon (Charmono) v1.1 genome