CuGenDBv2

Gene ID	Cmc03g0074741
Organism	Cucumis melo var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Reverse transcriptase
Genome location	CMiso1.1chr03:22187448..22187786
RNA-Seq Expression	Cmc03g0074741
Synteny	Cmc03g0074741
Gene Ontology terms	GO:0006278 - RNA-dependent DNA biosynthetic process (biological process) GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0006508 - proteolysis (biological process) GO:0015074 - DNA integration (biological process) GO:0043227 - membrane-bounded organelle (cellular component) GO:0003700 - DNA-binding transcription factor activity (molecular function) GO:0003964 - RNA-directed DNA polymerase activity (molecular function) GO:0004190 - aspartic-type endopeptidase activity (molecular function) GO:0008270 - zinc ion binding (molecular function) GO:0043565 - sequence-specific DNA binding (molecular function)
InterPro domains	IPR000477 - Reverse transcriptase domain IPR043128 - Reverse transcriptase/Diguanylate cyclase domain IPR043502 - DNA/RNA polymerase superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0036671.1 gag protease polyprotein [Cucumis melo var. makuwa]

2.6e-47

91.07

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        MSFGLTNAP VFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVL+T R NKLYAKFSKCEFWLK+VSFLGHVVSKAGVSVDP KIEAV   
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
        +RPSTVSEVRSF
Subjt:  SRPSTVSEVRSF

KAA0042119.1 pol protein [Cucumis melo var. makuwa]

8.9e-48

91.96

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        MSFGLTNAP VFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVL+T R NKLYAKFSKCEFWLK+VSFLGHVVSKAGVSVDP KIEAV S 
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
        +RPSTVSEVRSF
Subjt:  SRPSTVSEVRSF

KAA0054235.1 pol protein [Cucumis melo var. makuwa]

2.6e-47

91.07

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        MSFG TNAP VFMDLMNRVFREFLDTFVIVF+DDILIYSKTEAEHEEHLRMVLET  ANKLYAKFSKCEFWLK+VSFLGHVVSKAGVSVDPTKIEAV S 
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
         +PSTVSEVRSF
Subjt:  SRPSTVSEVRSF

KAA0058399.1 pol protein [Cucumis melo var. makuwa]

2.6e-47

91.07

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        MSFGLTNAP VFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVL+T R NKLYAKFSKCEFWLK+VSFLGHVVSKAGVSVDP KIEAV   
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
        +RPSTVSEVRSF
Subjt:  SRPSTVSEVRSF

KAA0060256.1 gag protease polyprotein [Cucumis melo var. makuwa]

2.6e-47

91.07

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        MSFGLTNAP VFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVL+T R NKLYAKFSKCEFWLK+VSFLGHVVSKAGVSVDP KIEAV   
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
        +RPSTVSEVRSF
Subjt:  SRPSTVSEVRSF

TrEMBL top hits

e value

%identity

Alignment

A0A5A7TLA3 Pol protein

4.3e-48

91.96

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        MSFGLTNAP VFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVL+T R NKLYAKFSKCEFWLK+VSFLGHVVSKAGVSVDP KIEAV S 
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
        +RPSTVSEVRSF
Subjt:  SRPSTVSEVRSF

A0A5A7TXE4 Reverse transcriptase

1.2e-47

91.07

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        MSFGLTNAP VFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVL+T R NKLYAKFSKCEFWLK+VSFLGHVVSKAGVSVDP KIEAV   
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
        +RPSTVSEVRSF
Subjt:  SRPSTVSEVRSF

A0A5A7UDB1 Reverse transcriptase

1.2e-47

91.07

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        MSFGLTNAP VFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVL+T R NKLYAKFSKCEFWLK+VSFLGHVVSKAGVSVDP KIEAV   
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
        +RPSTVSEVRSF
Subjt:  SRPSTVSEVRSF

A0A5A7VIT9 Reverse transcriptase

1.2e-47

91.07

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        MSFGLTNAP VFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVL+T R NKLYAKFSKCEFWLK+VSFLGHVVSKAGVSVDP KIEAV   
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
        +RPSTVSEVRSF
Subjt:  SRPSTVSEVRSF

A0A5D3CSZ9 Pol protein

1.2e-47

91.07

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        MSFG TNAP VFMDLMNRVFREFLDTFVIVF+DDILIYSKTEAEHEEHLRMVLET  ANKLYAKFSKCEFWLK+VSFLGHVVSKAGVSVDPTKIEAV S 
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
         +PSTVSEVRSF
Subjt:  SRPSTVSEVRSF

SwissProt top hits

e value

%identity

Alignment

P04323 Retrovirus-related Pol polyprotein from transposon 17.6

1.0e-14

36.61

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        M FGL NAP  F   MN + R  L+   +V++DDI+++S +  EH + L +V E      L  +  KCEF  ++ +FLGHV++  G+  +P KIEA+   
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
          P+   E+++F
Subjt:  SRPSTVSEVRSF

P0CT41 Transposon Tf2-12 polyprotein

2.1e-12

33.04

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        M +G++ AP  F   +N +  E  ++ V+ ++DDILI+SK+E+EH +H++ VL+  +   L    +KCEF   +V F+G+ +S+ G +     I+ V+  
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
         +P    E+R F
Subjt:  SRPSTVSEVRSF

P10401 Retrovirus-related Pol polyprotein from transposon gypsy

1.6e-12

33.93

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        + FGL NA  +F   ++ V RE +     V++DD++I+S+ E++H  H+  VL+      +     K  F+ + V +LG +VSK G   DP K++A+   
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
          P  V +VRSF
Subjt:  SRPSTVSEVRSF

P20825 Retrovirus-related Pol polyprotein from transposon 297

7.1e-16

36.61

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        M FGL NAP  F   MN + R  L+   +V++DDI+I+S +  EH   +++V        L  +  KCEF  K+ +FLGH+V+  G+  +P K++A++S 
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
          P+   E+R+F
Subjt:  SRPSTVSEVRSF

Q8I7P9 Retrovirus-related Pol polyprotein from transposon opus

2.5e-13

33.04

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Query:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL
        + FGL NAP +F  +++ + RE +     V+IDDI+++S+    H ++LR+VL +     L     K  F   +V FLG++V+  G+  DP K+ A+  +
Subjt:  MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISL

Query:  SRPSTVSEVRSF
          P++V E++ F
Subjt:  SRPSTVSEVRSF

Arabidopsis top hits

e value

%identity

Alignment

ATMG00860.1 DNA/RNA polymerases superfamily protein

2.5e-08

38.81

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Query:  HLRMVLETYRANKLYAKFSKCEFWLKKVSFLG--HVVSKAGVSVDPTKIEAVISLSRPSTVSEVRSF
        HL MVL+ +  ++ YA   KC F   ++++LG  H++S  GVS DP K+EA++    P   +E+R F
Subjt:  HLRMVLETYRANKLYAKFSKCEFWLKKVSFLG--HVVSKAGVSVDPTKIEAVISLSRPSTVSEVRSF

Sequences

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CDS sequence

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ATGTCTTTTGGTTTGACGAATGCTCCATTAGTGTTTATGGATTTGATGAACAGAGTGTTCAGGGAGTTCCTAGACACTTTTGTTATCGTGTTCATTGACGATATTTTGAT
ATATTCCAAGACAGAGGCAGAGCATGAGGAGCATTTACGCATGGTTCTAGAAACCTATCGAGCTAATAAATTGTATGCAAAGTTCTCAAAATGTGAGTTTTGGTTGAAGA
AGGTATCTTTTCTAGGCCATGTGGTTTCTAAAGCTGGTGTTTCTGTAGATCCAACTAAGATAGAGGCAGTCATCAGTTTGTCCCGACCTTCCACAGTAAGTGAGGTTCGT
AGCTTCTAG

mRNA sequence

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Protein sequence

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MSFGLTNAPLVFMDLMNRVFREFLDTFVIVFIDDILIYSKTEAEHEEHLRMVLETYRANKLYAKFSKCEFWLKKVSFLGHVVSKAGVSVDPTKIEAVISLSRPSTVSEVR
SF

Cmc03g0074741 (gene) of Melon (Charmono) v1.1 genome