CuGenDBv2

Gene ID	Cmc04g0094271
Organism	Cucumis melo var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
Description	Reverse transcriptase
Genome location	CMiso1.1chr04:6814352..6814756
RNA-Seq Expression	Cmc04g0094271
Synteny	Cmc04g0094271
Gene Ontology terms	GO:0006278 - RNA-dependent DNA biosynthetic process (biological process) GO:0006508 - proteolysis (biological process) GO:0015074 - DNA integration (biological process) GO:0003676 - nucleic acid binding (molecular function) GO:0003964 - RNA-directed DNA polymerase activity (molecular function) GO:0004190 - aspartic-type endopeptidase activity (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	IPR000477 - Reverse transcriptase domain IPR043128 - Reverse transcriptase/Diguanylate cyclase domain IPR043502 - DNA/RNA polymerase superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0051719.1 pol protein [Cucumis melo var. makuwa]

2.9e-61

89.55

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        MSF LTNAPAVFMDLMNRVFKDFLDSFVIVFIDDIL+YS+TEAEHEEHL+QVLETLRANKLYAKFSKCEFWL+KV+FLGHVV SEGVSVDPAK+EA+T+W
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P+ STVS+IRSFLGL GYYRRFVEDFSRIASPLT
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

KAA0061201.1 pol protein [Cucumis melo var. makuwa]

2.9e-61

89.55

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        MSF LTNAPAVFMDLMNRVFKDFLDSFVIVFIDDIL+YS+TEAEHEEHL+QVLETLRANKLYAKFSKCEFWL+KV+FLGHVV SEGVSVDPAK+EA+T+W
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P+ STVS+IRSFLGL GYYRRFVEDFSRIASPLT
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

KAA0063098.1 pol protein [Cucumis melo var. makuwa]

2.9e-61

89.55

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        MSF LTNAPAVFMDLMNRVFKDFLDSFVIVFIDDIL+YS+TEAEHEEHL+QVLETLRANKLYAKFSKCEFWL+KV+FLGHVV SEGVSVDPAK+EA+T+W
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P+ STVS+IRSFLGL GYYRRFVEDFSRIASPLT
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

KAA0063793.1 pol protein [Cucumis melo var. makuwa]

2.9e-61

89.55

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        MSF LTNAPAVFMDLMNRVFKDFLDSFVIVFIDDIL+YS+TEAEHEEHL+QVLETLRANKLYAKFSKCEFWL+KV+FLGHVV SEGVSVDPAK+EA+T+W
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P+ STVS+IRSFLGL GYYRRFVEDFSRIASPLT
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

KAA0066951.1 pol protein [Cucumis melo var. makuwa]

2.9e-61

89.55

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        MSF LTNAPAVFMDLMNRVFKDFLDSFVIVFIDDIL+YS+TEAEHEEHL+QVLETLRANKLYAKFSKCEFWL+KV+FLGHVV SEGVSVDPAK+EA+T+W
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P+ STVS+IRSFLGL GYYRRFVEDFSRIASPLT
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

TrEMBL top hits

e value

%identity

Alignment

A0A5A7UE01 Reverse transcriptase

1.4e-61

89.55

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        MSF LTNAPAVFMDLMNRVFKDFLDSFVIVFIDDIL+YS+TEAEHEEHL+QVLETLRANKLYAKFSKCEFWL+KV+FLGHVV SEGVSVDPAK+EA+T+W
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P+ STVS+IRSFLGL GYYRRFVEDFSRIASPLT
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

A0A5A7V4E4 Reverse transcriptase

1.4e-61

89.55

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        MSF LTNAPAVFMDLMNRVFKDFLDSFVIVFIDDIL+YS+TEAEHEEHL+QVLETLRANKLYAKFSKCEFWL+KV+FLGHVV SEGVSVDPAK+EA+T+W
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P+ STVS+IRSFLGL GYYRRFVEDFSRIASPLT
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

A0A5A7V646 Reverse transcriptase

1.4e-61

89.55

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        MSF LTNAPAVFMDLMNRVFKDFLDSFVIVFIDDIL+YS+TEAEHEEHL+QVLETLRANKLYAKFSKCEFWL+KV+FLGHVV SEGVSVDPAK+EA+T+W
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P+ STVS+IRSFLGL GYYRRFVEDFSRIASPLT
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

A0A5A7V6R2 Reverse transcriptase

1.4e-61

89.55

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        MSF LTNAPAVFMDLMNRVFKDFLDSFVIVFIDDIL+YS+TEAEHEEHL+QVLETLRANKLYAKFSKCEFWL+KV+FLGHVV SEGVSVDPAK+EA+T+W
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P+ STVS+IRSFLGL GYYRRFVEDFSRIASPLT
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

A0A5A7VMR4 Reverse transcriptase

1.4e-61

89.55

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        MSF LTNAPAVFMDLMNRVFKDFLDSFVIVFIDDIL+YS+TEAEHEEHL+QVLETLRANKLYAKFSKCEFWL+KV+FLGHVV SEGVSVDPAK+EA+T+W
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P+ STVS+IRSFLGL GYYRRFVEDFSRIASPLT
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

SwissProt top hits

e value

%identity

Alignment

P04323 Retrovirus-related Pol polyprotein from transposon 17.6

3.8e-24

41.04

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        M F L NAPA F   MN + +  L+   +V++DDI+V+S +  EH + L  V E L    L  +  KCEF  ++ +FLGHV+  +G+  +P K+EAI  +
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P  +   +I++FLGL GYYR+F+ +F+ IA P+T
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

P10401 Retrovirus-related Pol polyprotein from transposon gypsy

8.0e-22

36.57

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        + F L NA ++F   ++ V ++ +     V++DD++++SE E++H  H+  VL+ L    +     K  F+ + V +LG +V  +G   DP KV+AI  +
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P+   V K+RSFLGL  YYR F++DF+ IA P+T
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

P20825 Retrovirus-related Pol polyprotein from transposon 297

2.9e-24

40.3

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        M F L NAPA F   MN + +  L+   +V++DDI+++S +  EH   +  V   L    L  +  KCEF  K+ +FLGH+V  +G+  +P KV+AI S+
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P  +   +IR+FLGL GYYR+F+ +++ IA P+T
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

Q8I7P9 Retrovirus-related Pol polyprotein from transposon opus

2.1e-22

36.57

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        + F L NAPA+F  +++ + ++ +     V+IDDI+V+SE    H ++L  VL +L    L     K  F   +V FLG++V ++G+  DP KV AI+  
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        P  ++V +++ FLG+  YYR+F++D++++A PLT
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

Q99315 Transposon Ty3-G Gag-Pol polyprotein

5.7e-20

39.85

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Query:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW
        M F L NAP+ F   M   F+D    FV V++DDIL++SE+  EH +HL  VLE L+   L  K  KC+F  ++  FLG+ +  + ++    K  AI  +
Subjt:  MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSW

Query:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPL
        P   TV + + FLG++ YYRRF+ + S+IA P+
Subjt:  PQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPL

Arabidopsis top hits

e value

%identity

Alignment

ATMG00860.1 DNA/RNA polymerases superfamily protein

1.1e-18

44.94

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Query:  HLYQVLETLRANKLYAKFSKCEFWLKKVSFLG--HVVFSEGVSVDPAKVEAITSWPQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT
        HL  VL+    ++ YA   KC F   ++++LG  H++  EGVS DPAK+EA+  WP+    +++R FLGL GYYRRFV+++ +I  PLT
Subjt:  HLYQVLETLRANKLYAKFSKCEFWLKKVSFLG--HVVFSEGVSVDPAKVEAITSWPQSSTVSKIRSFLGLVGYYRRFVEDFSRIASPLT

Sequences

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CDS sequence

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ATGTCTTTTGAGTTGACTAATGCTCCTGCGGTATTCATGGACTTGATGAACAGGGTATTTAAGGATTTCTTAGACTCGTTCGTCATAGTTTTCATTGACGATATTTTGGT
TTACTCCGAGACTGAGGCTGAGCATGAAGAGCATTTATACCAGGTTTTGGAGACCCTTCGAGCCAATAAGTTGTATGCCAAGTTCTCCAAGTGTGAGTTCTGGCTGAAGA
AGGTATCTTTCCTTGGACATGTGGTCTTTAGTGAGGGAGTTTCTGTAGACCCAGCAAAGGTTGAAGCTATTACTAGTTGGCCTCAATCGTCGACGGTTAGCAAGATTCGT
AGTTTCCTGGGCTTGGTAGGTTACTACAGGAGGTTCGTGGAAGACTTCTCTCGTATAGCCAGCCCATTGACTTAG

mRNA sequence

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Protein sequence

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MSFELTNAPAVFMDLMNRVFKDFLDSFVIVFIDDILVYSETEAEHEEHLYQVLETLRANKLYAKFSKCEFWLKKVSFLGHVVFSEGVSVDPAKVEAITSWPQSSTVSKIR
SFLGLVGYYRRFVEDFSRIASPLT

Cmc04g0094271 (gene) of Melon (Charmono) v1.1 genome