CuGenDBv2

Gene ID	CmoCh00G001430
Organism	Cucurbita moschata Rifu (Cucurbita moschata (Rifu) v1)
Description	Reverse transcriptase
Genome location	Cmo_Chr00:25788762..25789304
RNA-Seq Expression	CmoCh00G001430
Synteny	CmoCh00G001430
Gene Ontology terms	GO:0006278 - RNA-dependent DNA biosynthetic process (biological process) GO:0006508 - proteolysis (biological process) GO:0015074 - DNA integration (biological process) GO:0090305 - nucleic acid phosphodiester bond hydrolysis (biological process) GO:0003676 - nucleic acid binding (molecular function) GO:0003964 - RNA-directed DNA polymerase activity (molecular function) GO:0004190 - aspartic-type endopeptidase activity (molecular function) GO:0004519 - endonuclease activity (molecular function) GO:0008194 - UDP-glycosyltransferase activity (molecular function) GO:0008270 - zinc ion binding (molecular function)
InterPro domains	IPR043128 - Reverse transcriptase/Diguanylate cyclase domain IPR043502 - DNA/RNA polymerase superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0055932.1 pol protein [Cucumis melo var. makuwa]

7.5e-65

68.85

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Query:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV
        + HG W +L +V+D R+   S+ +  VV+E+ DVFPDELP LPP +++DFAIELEP TTPIS+APYRMAP ELKELK QLQ+LLD GFIRPSVSPWGAPV
Subjt:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV

Query:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS
        L VKKKDG++RLCIDYRELNKVT+KN Y LPRIDDLFDQLQGA+VFSKIDLRSGYHQLRI++   P+     R    + V+MS
Subjt:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS

KAA0056047.1 reverse transcriptase [Cucumis melo var. makuwa]

9.8e-65

67.76

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Query:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV
        +  G W +LG+V+DTR+   S+ +  VV+E+ DVFPDELP LPP +++DFAIELEP T PIS+APYRMAPVELKELK QLQ+LLD GFIRPSVSPWGAPV
Subjt:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV

Query:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS
        L VKKKDG++RLCIDYRELNKVT+KN+Y LP+IDDLFDQLQGA+VFSKIDL+SGYHQLRI++   P+     R    + ++MS
Subjt:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS

KAA0063793.1 pol protein [Cucumis melo var. makuwa]

3.7e-64

67.76

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Query:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV
        + HG W +L +V+D R+   S+ +  VV+E+ DVFPD+LP LPP +++DFAIELEP T PIS+APYRMAP ELKELK QLQ+LLD GFIRPSVSPWGAPV
Subjt:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV

Query:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS
        L VKKKDG++RLCIDYRELNKVT+KN+Y LPRIDDLFDQLQGA+VFSKIDLRSGYHQLRI++   P+     R    + V+MS
Subjt:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS

XP_022931734.1 uncharacterized protein LOC111437896 [Cucurbita moschata]

9.8e-65

69.4

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Query:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV
        +  GAWA+L +V    K +  V +V VV EF+DVFP+ELP LPP +++DF I+LEP TTPISK PYRMAP ELKELK QLQ+LLD GFIRPSVSPWGAPV
Subjt:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV

Query:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS
        L VKKKDGT+RLCIDYRELNKVTIKNKY LPRIDDLFDQLQGA+VFSKIDLRSGYHQ+RI+E   P+     R    + ++MS
Subjt:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS

XP_022962669.1 uncharacterized protein LOC111463090 [Cucurbita moschata]

4.9e-64

70.49

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Query:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV
        +GHGA A L +V++  +    V  V VV EF DVFPD+LP LPP ++++F IELEP TTPISKAPYRMAP ELKELK QLQ+LL+ GFIRPSVSPWGAPV
Subjt:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV

Query:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS
        L VKKKDGTLRLCIDYRELNKVTIKNKY LPRIDDLFDQLQGA+VFSKIDLRSGYHQ+R+KE   P+     R    + V+MS
Subjt:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS

TrEMBL top hits

e value

%identity

Alignment

A0A5A7UJ81 Reverse transcriptase

3.6e-65

68.85

Show/hide

Query:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV
        + HG W +L +V+D R+   S+ +  VV+E+ DVFPDELP LPP +++DFAIELEP TTPIS+APYRMAP ELKELK QLQ+LLD GFIRPSVSPWGAPV
Subjt:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV

Query:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS
        L VKKKDG++RLCIDYRELNKVT+KN Y LPRIDDLFDQLQGA+VFSKIDLRSGYHQLRI++   P+     R    + V+MS
Subjt:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS

A0A5A7UN68 Reverse transcriptase

4.8e-65

67.76

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Query:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV
        +  G W +LG+V+DTR+   S+ +  VV+E+ DVFPDELP LPP +++DFAIELEP T PIS+APYRMAPVELKELK QLQ+LLD GFIRPSVSPWGAPV
Subjt:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV

Query:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS
        L VKKKDG++RLCIDYRELNKVT+KN+Y LP+IDDLFDQLQGA+VFSKIDL+SGYHQLRI++   P+     R    + ++MS
Subjt:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS

A0A5A7V6R2 Reverse transcriptase

1.8e-64

67.76

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Query:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV
        + HG W +L +V+D R+   S+ +  VV+E+ DVFPD+LP LPP +++DFAIELEP T PIS+APYRMAP ELKELK QLQ+LLD GFIRPSVSPWGAPV
Subjt:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV

Query:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS
        L VKKKDG++RLCIDYRELNKVT+KN+Y LPRIDDLFDQLQGA+VFSKIDLRSGYHQLRI++   P+     R    + V+MS
Subjt:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS

A0A6J1EV26 Reverse transcriptase

4.8e-65

69.4

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Query:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV
        +  GAWA+L +V    K +  V +V VV EF+DVFP+ELP LPP +++DF I+LEP TTPISK PYRMAP ELKELK QLQ+LLD GFIRPSVSPWGAPV
Subjt:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV

Query:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS
        L VKKKDGT+RLCIDYRELNKVTIKNKY LPRIDDLFDQLQGA+VFSKIDLRSGYHQ+RI+E   P+     R    + ++MS
Subjt:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS

A0A6J1HHR1 uncharacterized protein LOC111463090

2.4e-64

70.49

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Query:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV
        +GHGA A L +V++  +    V  V VV EF DVFPD+LP LPP ++++F IELEP TTPISKAPYRMAP ELKELK QLQ+LL+ GFIRPSVSPWGAPV
Subjt:  MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPV

Query:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS
        L VKKKDGTLRLCIDYRELNKVTIKNKY LPRIDDLFDQLQGA+VFSKIDLRSGYHQ+R+KE   P+     R    + V+MS
Subjt:  LSVKKKDGTLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPR-----RLSGADMVIMS

SwissProt top hits

e value

%identity

Alignment

P0CT34 Transposon Tf2-1 polyprotein

4.5e-20

34.78

Show/hide

Query:  VVQEFKDVFPD-ELPRLP-PVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPVLSVKKKDGTLRLCIDYRELNKVTI
        + +EFKD+  +    +LP P+K ++F +EL      +    Y + P +++ + +++   L  G IR S +    PV+ V KK+GTLR+ +DY+ LNK   
Subjt:  VVQEFKDVFPD-ELPRLP-PVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPVLSVKKKDGTLRLCIDYRELNKVTI

Query:  KNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKE
         N Y LP I+ L  ++QG+++F+K+DL+S YH +R+++
Subjt:  KNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKE

P0CT41 Transposon Tf2-12 polyprotein

4.5e-20

34.78

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Query:  VVQEFKDVFPD-ELPRLP-PVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPVLSVKKKDGTLRLCIDYRELNKVTI
        + +EFKD+  +    +LP P+K ++F +EL      +    Y + P +++ + +++   L  G IR S +    PV+ V KK+GTLR+ +DY+ LNK   
Subjt:  VVQEFKDVFPD-ELPRLP-PVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPVLSVKKKDGTLRLCIDYRELNKVTI

Query:  KNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKE
         N Y LP I+ L  ++QG+++F+K+DL+S YH +R+++
Subjt:  KNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKE

Q7LHG5 Transposon Ty3-I Gag-Pol polyprotein

3.5e-25

40.67

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Query:  TRKDTRSVMNVLVVQEFKDVFPDELPRLP------PVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPVLSVKKKDG
        + KDT   + V + Q+++++  ++LP  P      PVK     IE++P        PY +     +E+ + +Q LLD  FI PS SP  +PV+ V KKDG
Subjt:  TRKDTRSVMNVLVVQEFKDVFPDELPRLP------PVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPVLSVKKKDG

Query:  TLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQL
        T RLC+DYR LNK TI + + LPRID+L  ++  A +F+ +DL SGYHQ+
Subjt:  TLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQL

Q99315 Transposon Ty3-G Gag-Pol polyprotein

3.5e-25

40.67

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Query:  TRKDTRSVMNVLVVQEFKDVFPDELPRLP------PVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPVLSVKKKDG
        + KDT   + V + Q+++++  ++LP  P      PVK     IE++P        PY +     +E+ + +Q LLD  FI PS SP  +PV+ V KKDG
Subjt:  TRKDTRSVMNVLVVQEFKDVFPDELPRLP------PVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPVLSVKKKDG

Query:  TLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQL
        T RLC+DYR LNK TI + + LPRID+L  ++  A +F+ +DL SGYHQ+
Subjt:  TLRLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQL

Q9UR07 Transposon Tf2-11 polyprotein

4.5e-20

34.78

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Query:  VVQEFKDVFPD-ELPRLP-PVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPVLSVKKKDGTLRLCIDYRELNKVTI
        + +EFKD+  +    +LP P+K ++F +EL      +    Y + P +++ + +++   L  G IR S +    PV+ V KK+GTLR+ +DY+ LNK   
Subjt:  VVQEFKDVFPD-ELPRLP-PVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPVLSVKKKDGTLRLCIDYRELNKVTI

Query:  KNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKE
         N Y LP I+ L  ++QG+++F+K+DL+S YH +R+++
Subjt:  KNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKE

Arabidopsis top hits	e value	%identity	Alignment
No hits found

Sequences

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CDS sequence

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ATGGGCCACGGAGCTTGGGCCCTATTGGGCAACGTAATGGACACGAGGAAAGATACTCGTAGTGTGATGAACGTGCTTGTAGTACAAGAGTTTAAAGATGTGTTTCCAGA
TGAACTGCCTAGGTTACCACCCGTCAAGGACATGGACTTTGCAATCGAACTGGAACCTAGTACAACTCCAATCTCCAAGGCACCTTACAGAATGGCTCCTGTCGAGTTGA
AGGAACTTAAGGAACAATTGCAGGATCTCTTAGATTGGGGTTTTATCAGACCAAGTGTATCGCCATGGGGAGCCCCAGTGCTTTCTGTCAAGAAGAAGGATGGGACCTTG
AGATTGTGCATTGACTATAGGGAATTGAATAAAGTAACAATAAAAAACAAATACTCGTTGCCTCGCATTGATGATCTATTTGATCAATTGCAAGGTGCTTCAGTATTTTC
AAAAATTGACCTACGATCGGGATACCATCAGTTGAGGATAAAGGAGGTGATATCCCCAAGACGGCTTTCAGGAGCCGATATGGTCATTATGAGTTTACCGTGA

mRNA sequence

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Protein sequence

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MGHGAWALLGNVMDTRKDTRSVMNVLVVQEFKDVFPDELPRLPPVKDMDFAIELEPSTTPISKAPYRMAPVELKELKEQLQDLLDWGFIRPSVSPWGAPVLSVKKKDGTL
RLCIDYRELNKVTIKNKYSLPRIDDLFDQLQGASVFSKIDLRSGYHQLRIKEVISPRRLSGADMVIMSLP

CmoCh00G001430 (gene) of Cucurbita moschata (Rifu) v1 genome