CuGenDBv2

Gene ID	CmoCh02G004150
Organism	Cucurbita moschata Rifu (Cucurbita moschata (Rifu) v1)
Description	mediator of RNA polymerase II transcription subunit 32-like
Genome location	Cmo_Chr02:2140887..2142819
RNA-Seq Expression	CmoCh02G004150
Synteny	CmoCh02G004150
Gene Ontology terms	GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0009631 - cold acclimation (biological process) GO:0010150 - leaf senescence (biological process) GO:0048364 - root development (biological process) GO:0016592 - mediator complex (cellular component)
InterPro domains	IPR033244 - Mediator of RNA polymerase II transcription subunit 32

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6605089.1 Mediator of RNA polymerase II transcription subunit 32, partial [Cucurbita argyrosperma subsp. sororia]

5.3e-72

99.35

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Query:  RYSDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSS
        RYSDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSS 
Subjt:  RYSDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSS

Query:  LPPISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
        LPPISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
Subjt:  LPPISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ

KAG7035096.1 Mediator of RNA polymerase II transcription subunit 32, partial [Cucurbita argyrosperma subsp. argyrosperma]

1.4e-72

98.06

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Query:  MYRYSDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATS
        ++RYSDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATS
Subjt:  MYRYSDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATS

Query:  SSLPPISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
        S LPPISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
Subjt:  SSLPPISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ

XP_022948111.1 mediator of RNA polymerase II transcription subunit 32-like [Cucurbita moschata]

6.5e-70

100

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Query:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLPPI
        MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLPPI
Subjt:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLPPI

Query:  SAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
        SAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
Subjt:  SAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ

XP_023007274.1 mediator of RNA polymerase II transcription subunit 32-like [Cucurbita maxima]

2.4e-64

93.51

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Query:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQA-AAATSSSLPP
        MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQA AAAT++ LP 
Subjt:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQA-AAATSSSLPP

Query:  ISAIRLEQMSKAVRWLVIELQHGSG----AAASASAHSHSSAPFDARFSEDTTQ
        ISAIRLEQMSKAVRWLVIELQHGSG    A+ASASAHSHSSAPFDARFSEDTTQ
Subjt:  ISAIRLEQMSKAVRWLVIELQHGSG----AAASASAHSHSSAPFDARFSEDTTQ

XP_023534665.1 mediator of RNA polymerase II transcription subunit 32-like [Cucurbita pepo subsp. pepo]

7.2e-69

98.66

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Query:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLPPI
        MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSS LPPI
Subjt:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLPPI

Query:  SAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
        SAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHS APFDARFSEDTTQ
Subjt:  SAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ

TrEMBL top hits

e value

%identity

Alignment

A0A5A7TWZ7 Mediator of RNA polymerase II transcription subunit 32

1.9e-62

88.08

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Query:  SDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLP
        SDMDSLVDSLNNAYQEFVAGAA+VLEANE SGAQKTAATDAALENFKQKWELF VACDQAEEFVESVKQRIGSECL+DEATGSL+ KSGQ+    ++ LP
Subjt:  SDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLP

Query:  PISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
        PISA+RLEQMSKAVRWLVIELQHGSGAAASASAHSH SAPFDARF+ED TQ
Subjt:  PISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ

A0A5D3CQ46 Mediator of RNA polymerase II transcription subunit 32

4.9e-63

88.74

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Query:  SDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLP
        SDMDSLVDSLNNAYQEFVAGAA+VLEANE SGAQKTAATDAALENFKQKWELF VACDQAEEFVESVKQRIGSECL+DEATGSL+ KSGQ+    ++ LP
Subjt:  SDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLP

Query:  PISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
        PISA+RLEQMSKAVRWLVIELQHGSGAAASASAHSH SAPFDARF+EDTTQ
Subjt:  PISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ

A0A6J1G8D0 mediator of RNA polymerase II transcription subunit 32-like

3.2e-70

100

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Query:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLPPI
        MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLPPI
Subjt:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLPPI

Query:  SAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
        SAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
Subjt:  SAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ

A0A6J1JEJ1 mediator of RNA polymerase II transcription subunit 32-like isoform X1

1.1e-62

90.13

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Query:  SDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLP
        SDMDSLVDSLNNAYQEFV+GAA+VLEANE SGAQKTAATDAALENFKQKWELF VACDQAEEFVESVKQRIGSECL+DEATGSL+ KSGQ AAAT++ LP
Subjt:  SDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLP

Query:  PISAIRLEQMSKAVRWLVIELQHGSG-AAASASAHSHSSAPFDARFSEDTTQ
        PISAIRLEQMSKAVRWLVIELQHGSG AAASASAHSH SAPFDARF+ED TQ
Subjt:  PISAIRLEQMSKAVRWLVIELQHGSG-AAASASAHSHSSAPFDARFSEDTTQ

A0A6J1L2I3 mediator of RNA polymerase II transcription subunit 32-like

1.2e-64

93.51

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Query:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQA-AAATSSSLPP
        MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQA AAAT++ LP 
Subjt:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATGSLSSKSGQA-AAATSSSLPP

Query:  ISAIRLEQMSKAVRWLVIELQHGSG----AAASASAHSHSSAPFDARFSEDTTQ
        ISAIRLEQMSKAVRWLVIELQHGSG    A+ASASAHSHSSAPFDARFSEDTTQ
Subjt:  ISAIRLEQMSKAVRWLVIELQHGSG----AAASASAHSHSSAPFDARFSEDTTQ

SwissProt top hits

e value

%identity

Alignment

Q84VW5 Mediator of RNA polymerase II transcription subunit 32

2.6e-45

67.97

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Query:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATG-SLSSKSGQAAAA---TSSS
        MD++VDSLN AY++FV  +A VLE+ E++G QK   TD ALENFK+KWELF VACDQAEEFVESVKQRIGSECL+DEATG + ++  GQA AA    ++S
Subjt:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATG-SLSSKSGQAAAA---TSSS

Query:  LPPISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
        LPPISA+RLEQMS+AVRWLV+ELQ GSG  A  S HS SS  FD+RFSED+TQ
Subjt:  LPPISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ

Arabidopsis top hits

e value

%identity

Alignment

AT1G11760.1 MED32; Has 41 Blast hits to 40 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).

1.8e-46

67.97

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Query:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATG-SLSSKSGQAAAA---TSSS
        MD++VDSLN AY++FV  +A VLE+ E++G QK   TD ALENFK+KWELF VACDQAEEFVESVKQRIGSECL+DEATG + ++  GQA AA    ++S
Subjt:  MDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEFVESVKQRIGSECLIDEATG-SLSSKSGQAAAA---TSSS

Query:  LPPISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ
        LPPISA+RLEQMS+AVRWLV+ELQ GSG  A  S HS SS  FD+RFSED+TQ
Subjt:  LPPISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ

Sequences

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CDS sequence

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ATGTATAGGTATTCTGAATCAAAAGCTGAGTGGACAATATCATCCAACTGTGAATATGCTAGCTTAACTGAAAGTTCGAAATCTAGCTTGTGCATTTTGGACTTGAAATG
CTTACTTTTTGGCTATCATATGTATAGGTATTCTGATATGGACAGCCTAGTGGACTCTTTAAATAACGCATATCAGGAGTTTGTTGCTGGAGCAGCTAGCGTCCTTGAAG
CAAACGAAACCTCTGGAGCCCAGAAGACAGCAGCAACAGACGCAGCTCTTGAGAACTTTAAACAGAAATGGGAGCTATTCATGGTTGCTTGTGATCAAGCAGAGGAGTTT
GTGGAGTCTGTGAAGCAAAGGATAGGATCAGAGTGCCTGATTGATGAGGCCACGGGTTCACTGTCCAGCAAGTCTGGGCAGGCCGCCGCTGCCACGTCTTCTAGCCTTCC
TCCGATCAGTGCCATTCGGCTCGAACAGATGAGCAAAGCTGTGAGATGGCTTGTGATTGAGTTGCAGCATGGCTCCGGAGCTGCTGCTTCTGCTTCAGCACACTCCCACT
CTTCCGCTCCTTTTGACGCTCGATTTAGCGAGGATACAACCCAATAG

mRNA sequence

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Protein sequence

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MYRYSESKAEWTISSNCEYASLTESSKSSLCILDLKCLLFGYHMYRYSDMDSLVDSLNNAYQEFVAGAASVLEANETSGAQKTAATDAALENFKQKWELFMVACDQAEEF
VESVKQRIGSECLIDEATGSLSSKSGQAAAATSSSLPPISAIRLEQMSKAVRWLVIELQHGSGAAASASAHSHSSAPFDARFSEDTTQ

CmoCh02G004150 (gene) of Cucurbita moschata (Rifu) v1 genome