CuGenDBv2

Gene ID	CmoCh07G006040
Organism	Cucurbita moschata Rifu (Cucurbita moschata (Rifu) v1)
Description	tRNA-dihydrouridine synthase
Genome location	Cmo_Chr07:2721948..2723017
RNA-Seq Expression	CmoCh07G006040
Synteny	CmoCh07G006040
Gene Ontology terms	GO:0002943 - tRNA dihydrouridine synthesis (biological process) GO:0005737 - cytoplasm (cellular component) GO:0017150 - tRNA dihydrouridine synthase activity (molecular function) GO:0050660 - flavin adenine dinucleotide binding (molecular function)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6594928.1 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like protein, partial [Cucurbita argyrosperma subsp. sororia]

5.8e-48

100

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
        MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR

KAG7026892.1 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like protein [Cucurbita argyrosperma subsp. argyrosperma]

5.8e-48

100

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
        MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR

XP_022962639.1 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita moschata]

5.8e-48

100

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
        MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR

XP_022962640.1 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X2 [Cucurbita moschata]

5.8e-48

100

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
        MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR

XP_023003695.1 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita maxima]

2.2e-47

98.96

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
        MVARGALWNASIFS DGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR

TrEMBL top hits

e value

%identity

Alignment

A0A6J1BSD4 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1

3.8e-45

93.75

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
        MVARGALWNASIFS +GKLPWE+VKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKS++LADLAKLYGEEKYN FVQEKRLFDGSR
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR

A0A6J1HFN6 tRNA-dihydrouridine synthase

2.8e-48

100

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
        MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR

A0A6J1HHN5 tRNA-dihydrouridine synthase

2.8e-48

100

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
        MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR

A0A6J1KNB4 tRNA-dihydrouridine synthase

1.1e-47

98.96

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
        MVARGALWNASIFS DGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR

A0A6J1KSI8 tRNA-dihydrouridine synthase

1.1e-47

98.96

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
        MVARGALWNASIFS DGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR

SwissProt top hits

e value

%identity

Alignment

Q9D7B1 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like

2.5e-09

37.18

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYG
        MVAR A+WN SIF  DG  P EEV ++Y+R ++ +DN   +TK+ L +M+     LE P+G+ +  + +  ++ + +G
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYG

Q9NX74 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like

7.2e-09

35.9

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYG
        MVAR A+WN SIF  +G  P EEV ++Y+R ++ +DN   +TK+ L +M+     LE P+G+ +  + +  ++ + +G
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYG

Arabidopsis top hits

e value

%identity

Alignment

AT3G49640.1 Aldolase-type TIM barrel family protein

6.9e-31

70.73

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKY
        MVARGA+WNASIFS  GK  WE+VK++Y+RKSILW+ND+KSTK+T+KEMI H+SCLELPEGK++ K+DTL DLA+LY  E Y
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKY

AT3G49640.2 Aldolase-type TIM barrel family protein

6.9e-31

70.73

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Query:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKY
        MVARGA+WNASIFS  GK  WE+VK++Y+RKSILW+ND+KSTK+T+KEMI H+SCLELPEGK++ K+DTL DLA+LY  E Y
Subjt:  MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKY

Sequences

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CDS sequence

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mRNA sequence

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ATGGTTGCTCGAGGAGCGCTTTGGAACGCTTCAATCTTCTCTGCAGATGGCAAATTACCATGGGAAGAGGTGAAAAGAGAATATGTTAGAAAGAGCATCTTGTGGGACAA
TGATATCAAAAGTACAAAGCACACGCTAAAAGAAATGATAATGCATTATTCTTGCCTTGAACTGCCAGAAGGAAAGGCCGTGATAAAATCTGATACATTGGCAGATCTAG
CGAAACTTTATGGCGAGGAAAAGTACAATCAATTTGTTCAAGAGAAGCGTTTGTTCGATGGAAGTAGATGACGTCGTTCGTAGTTCGGATCAGAGACGCTGTTCATTGCT
TCGACATGGAACAACCTCGAGTTATTAAGCTGCCGCAGATGATGCCTGGCTTTATTTGCTTTTCTGGAATGCGTATTTATGGTTCTAGGATGTTCCAAGTTGAAAGCTTT
GGCTTATTGTTGCCAAATTCATCCAGTTCGTTGATTTCAGTACCCATCAATCAATCAATCAATGGGTTAATATCTTTTAAAATTAGTCTTGGGATAAAAACACTAATCGT
GAATTCATTTTACTCATAAACATTTGACCAAATTGACACGATCCATACCATAAGTTTGCCAAATTTTCTATCTAATTAC

Protein sequence

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MVARGALWNASIFSADGKLPWEEVKREYVRKSILWDNDIKSTKHTLKEMIMHYSCLELPEGKAVIKSDTLADLAKLYGEEKYNQFVQEKRLFDGSR

CmoCh07G006040 (gene) of Cucurbita moschata (Rifu) v1 genome