CuGenDBv2

Gene ID	CmoCh07G006050
Organism	Cucurbita moschata Rifu (Cucurbita moschata (Rifu) v1)
Description	tRNA-dihydrouridine synthase
Genome location	Cmo_Chr07:2723227..2723697
RNA-Seq Expression	CmoCh07G006050
Synteny	CmoCh07G006050
Gene Ontology terms	GO:0002943 - tRNA dihydrouridine synthesis (biological process) GO:0017150 - tRNA dihydrouridine synthase activity (molecular function) GO:0050660 - flavin adenine dinucleotide binding (molecular function)
InterPro domains	IPR013785 - Aldolase-type TIM barrel IPR035587 - DUS-like, FMN-binding domain

Homology

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GenBank top hits

e value

%identity

Alignment

KAG7026892.1 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like protein [Cucurbita argyrosperma subsp. argyrosperma]

3.6e-17

100

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Query:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Subjt:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

XP_022962639.1 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita moschata]

3.6e-17

100

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Query:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Subjt:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

XP_023003695.1 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita maxima]

3.6e-17

100

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Query:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Subjt:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

XP_023517246.1 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X1 [Cucurbita pepo subsp. pepo]

3.6e-17

100

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Query:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Subjt:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

XP_023517247.1 tRNA-dihydrouridine(20) synthase [NAD(P)+]-like isoform X2 [Cucurbita pepo subsp. pepo]

3.6e-17

100

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Query:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Subjt:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

TrEMBL top hits

e value

%identity

Alignment

A0A5B6YTB2 Putative tRNA-dihydrouridine(20) synthase [NAD(P)+]-like (Fragment)

5.6e-16

91.3

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Query:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        +VADRPRDPAKWSEIADVVAALS+PVIANGD+FEYEDF HIKVATG
Subjt:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

A0A6J1HFN6 tRNA-dihydrouridine synthase

1.7e-17

100

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Query:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Subjt:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

A0A6J1HHN5 tRNA-dihydrouridine synthase

1.7e-17

100

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Query:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Subjt:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

A0A6J1KNB4 tRNA-dihydrouridine synthase

1.7e-17

100

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Query:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Subjt:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

A0A6J1KSI8 tRNA-dihydrouridine synthase

1.7e-17

100

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Query:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
Subjt:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

SwissProt top hits

e value

%identity

Alignment

O68273 tRNA-dihydrouridine(16) synthase

8.1e-04

40.62

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Query:  CYQACSEG--RLLRVADRPRD-----PAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        C  A +EG    L V  R RD     PA W  IA + AA+ VPVIANGD++   D+   +  +G
Subjt:  CYQACSEG--RLLRVADRPRD-----PAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

Arabidopsis top hits

e value

%identity

Alignment

AT3G49640.1 Aldolase-type TIM barrel family protein

1.5e-16

78.26

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Query:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        ++ADRPRDPAKW EIADVVAALS+PVIANGD+ EY+DF  IK ATG
Subjt:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

AT3G49640.2 Aldolase-type TIM barrel family protein

1.5e-16

78.26

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Query:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG
        ++ADRPRDPAKW EIADVVAALS+PVIANGD+ EY+DF  IK ATG
Subjt:  RVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATG

Sequences

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CDS sequence

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ATGTGCTACCAAGCATGCTCAGAGGGCAGACTACTAAGAGTTGCAGATAGGCCCAGAGATCCAGCTAAGTGGAGTGAGATTGCAGATGTCGTGGCAGCATTGTCTGTCCC
GGTTATTGCGAACGGTGATATCTTTGAGTACGAAGATTTTCACCATATCAAAGTTGCTACAGGTTTGCTCAAAAGGCACTGTTTTATGGATTTCTTGATGATGCCTGAAT
TTCTTTTAGTGAATGGGTTGAATATGATCCCAACCTGA

mRNA sequence

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Protein sequence

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MCYQACSEGRLLRVADRPRDPAKWSEIADVVAALSVPVIANGDIFEYEDFHHIKVATGLLKRHCFMDFLMMPEFLLVNGLNMIPT

CmoCh07G006050 (gene) of Cucurbita moschata (Rifu) v1 genome