CuGenDBv2

Gene ID	CmoCh07G007950
Organism	Cucurbita moschata Rifu (Cucurbita moschata (Rifu) v1)
Description	crossover junction endonuclease EME1B-like isoform X1
Genome location	Cmo_Chr07:3639776..3643120
RNA-Seq Expression	CmoCh07G007950
Synteny	CmoCh07G007950
Gene Ontology terms	GO:0006281 - DNA repair (biological process) GO:0006310 - DNA recombination (biological process) GO:0007049 - cell cycle (biological process) GO:0009416 - response to light stimulus (biological process) GO:0051301 - cell division (biological process) GO:0090305 - nucleic acid phosphodiester bond hydrolysis (biological process) GO:0005634 - nucleus (cellular component) GO:0048476 - Holliday junction resolvase complex (cellular component) GO:0003677 - DNA binding (molecular function) GO:0004519 - endonuclease activity (molecular function)
InterPro domains	IPR033310 - Mms4/EME1/EME2 IPR042530 - EME1/EME2, C-terminal domain

Homology

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GenBank top hits

e value

%identity

Alignment

XP_022950534.1 crossover junction endonuclease EME1B-like isoform X2 [Cucurbita moschata]

4.1e-59

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Query:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV
        +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHLSVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V
Subjt:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV

Query:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
         IWKKYPTMKS   VYM+PCKSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Subjt:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

XP_022950535.1 crossover junction endonuclease EME1B-like isoform X3 [Cucurbita moschata]

4.1e-59

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Query:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV
        +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHLSVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V
Subjt:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV

Query:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
         IWKKYPTMKS   VYM+PCKSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Subjt:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

XP_022977602.1 crossover junction endonuclease EME1B isoform X1 [Cucurbita maxima]

4.1e-59

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Query:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV
        +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHLSVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V
Subjt:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV

Query:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
         IWKKYPTMKS   VYM+PCKSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Subjt:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

XP_022977603.1 crossover junction endonuclease EME1B isoform X2 [Cucurbita maxima]

4.1e-59

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Query:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV
        +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHLSVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V
Subjt:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV

Query:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
         IWKKYPTMKS   VYM+PCKSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Subjt:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

XP_022977604.1 crossover junction endonuclease EME1B isoform X3 [Cucurbita maxima]

4.1e-59

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Query:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV
        +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHLSVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V
Subjt:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV

Query:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
         IWKKYPTMKS   VYM+PCKSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Subjt:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

TrEMBL top hits

e value

%identity

Alignment

A0A6J1GF29 crossover junction endonuclease EME1B-like isoform X1

2.0e-59

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Query:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV
        +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHLSVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V
Subjt:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV

Query:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
         IWKKYPTMKS   VYM+PCKSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Subjt:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

A0A6J1GG33 crossover junction endonuclease EME1B-like isoform X2

2.0e-59

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Query:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV
        +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHLSVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V
Subjt:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV

Query:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
         IWKKYPTMKS   VYM+PCKSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Subjt:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

A0A6J1IIZ2 crossover junction endonuclease EME1B isoform X1

2.0e-59

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Query:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV
        +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHLSVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V
Subjt:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV

Query:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
         IWKKYPTMKS   VYM+PCKSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Subjt:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

A0A6J1IQH9 crossover junction endonuclease EME1B isoform X2

2.0e-59

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Query:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV
        +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHLSVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V
Subjt:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV

Query:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
         IWKKYPTMKS   VYM+PCKSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Subjt:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

A0A6J1IRV2 crossover junction endonuclease EME1B isoform X3

2.0e-59

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Query:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV
        +LAKLTTHFVKVRSRQCVDE E AD    LTCSLAS QF              RKKLTHLSVNA GSIIPKDC+ RN+I+K + L+ LVAIPKVQPRF+V
Subjt:  KLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVLVAIPKVQPRFSV

Query:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
         IWKKYPTMKS   VYM+PCKSVHEKEFLLKD T EGLLG+DRRLGE+CSKRVYRILMAQSGSMRTDD
Subjt:  PIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

SwissProt top hits

e value

%identity

Alignment

C5H8J1 Crossover junction endonuclease EME1B

4.1e-46

54.84

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Query:  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKC
        +Y  PGN R     +    LAKLTTH+VKV SR CVDE E A+    LT SLAS QF              RKKLT LSV+A G+++ KD V +++I+K 
Subjt:  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKC

Query:  VRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
          L+ LVAIPKVQPR+++ +WKKYP+MKS  +VYM+  KSVHEKEFLLKD   EGL+G D RLGEICSKR+YR+LM+  G+++TDD
Subjt:  VRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

Q0J9J6 Crossover junction endonuclease EME1

1.9e-30

46.67

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Query:  NRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVL
        N  S +       L KL TH+ +VRSR C DE E  +    LT SLA+ +F              RK LT LSV+A GS I K CV ++ I+K   L+ L
Subjt:  NRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKCVRLEVL

Query:  VAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGN-DRRLGEICSKRVYRILMAQSGSMRTD
        VAIP V P  ++ I KKYP+M+S   VYM+  KSVHEKE LL+D   EG LG+  RRLG  CSK+VY ILMAQ+G+   +
Subjt:  VAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGN-DRRLGEICSKRVYRILMAQSGSMRTD

Q84M98 Crossover junction endonuclease EME1A

1.4e-38

47.31

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Query:  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAAD---ILTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKC
        +Y  P N    R       +AKL+TH++ V SR CVDE E AD    LT SLA  Q              +RKKLT LSV A G+++ K+   +++I + 
Subjt:  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAAD---ILTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKC

Query:  VRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
        + L+VLVAIPKVQPR+++ + KKYP++KS  +VYM+P  SVHEKEFLLKD   E L+G D  +GE CSKR+YR+LM+  G+++TDD
Subjt:  VRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

Arabidopsis top hits

e value

%identity

Alignment

AT2G21800.1 essential meiotic endonuclease 1A

1.0e-39

47.31

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Query:  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAAD---ILTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKC
        +Y  P N    R       +AKL+TH++ V SR CVDE E AD    LT SLA  Q              +RKKLT LSV A G+++ K+   +++I + 
Subjt:  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAAD---ILTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKC

Query:  VRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
        + L+VLVAIPKVQPR+++ + KKYP++KS  +VYM+P  SVHEKEFLLKD   E L+G D  +GE CSKR+YR+LM+  G+++TDD
Subjt:  VRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

AT2G21800.2 essential meiotic endonuclease 1A

1.0e-39

47.31

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Query:  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAAD---ILTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKC
        +Y  P N    R       +AKL+TH++ V SR CVDE E AD    LT SLA  Q              +RKKLT LSV A G+++ K+   +++I + 
Subjt:  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAAD---ILTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKC

Query:  VRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
        + L+VLVAIPKVQPR+++ + KKYP++KS  +VYM+P  SVHEKEFLLKD   E L+G D  +GE CSKR+YR+LM+  G+++TDD
Subjt:  VRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

AT2G22140.1 essential meiotic endonuclease 1B

2.9e-47

54.84

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Query:  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKC
        +Y  PGN R     +    LAKLTTH+VKV SR CVDE E A+    LT SLAS QF              RKKLT LSV+A G+++ KD V +++I+K 
Subjt:  QYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHRNVIEKC

Query:  VRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD
          L+ LVAIPKVQPR+++ +WKKYP+MKS  +VYM+  KSVHEKEFLLKD   EGL+G D RLGEICSKR+YR+LM+  G+++TDD
Subjt:  VRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDD

Sequences

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CDS sequence

Show/hide CDS sequence

ATGAAAGTTCTGTTTCATTTAGAAGAAAATATATTTATTACATGCTCTTTGCAATTTCAGTATCTTTGTCCTGGGAACAGGAGAAGTACAAGAACTCAGAAAGCAATAAC
GAAATTGGCAAAACTGACCACTCACTTTGTCAAGGTACGTTCTAGGCAGTGTGTAGATGAAACTGAGGCAGCTGACATATTGACATGCAGTCTGGCATCCTTTCAATTCA
GTCAGAATTATCTATTTTCCTTTGCTTGTCTTGTAAAATTGAGGAAAAAGTTGACGCATCTATCCGTCAATGCAATTGGATCCATTATACCCAAAGATTGTGTTCACAGG
AATGTGATAGAGAAGTGCGTGAGGTTAGAAGTCTTGGTAGCAATTCCAAAGGTACAACCACGGTTTTCCGTACCAATATGGAAGAAATATCCGACCATGAAATCTTTTTC
GAGAGTGTACATGAATCCATGTAAATCTGTGCATGAAAAGGAATTTCTGCTGAAAGACTGGACGACAGAAGGTTTACTCGGCAATGATAGAAGGTTAGGAGAGATTTGTT
CGAAGAGAGTGTACAGGATACTTATGGCTCAAAGTGGAAGCATGAGAACTGATGACAGTTTTCACCCTCGCAGACAGGTTGAAGGACTAGAGTGCCCTCATCTCTTTATC
ACAAATGCCACTTGGAGGCAGCGACTCGTGGAGCAAAGGGTAATTGTTGCATCTTTCATAGAAGATCTCTGGACATTAGAATATAGGAAATTAAAATTCACATTCGCAAT
ACTCATTTTGGTGCTTCGCACTTTCGCAAAGTGA

mRNA sequence

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Protein sequence

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MKVLFHLEENIFITCSLQFQYLCPGNRRSTRTQKAITKLAKLTTHFVKVRSRQCVDETEAADILTCSLASFQFSQNYLFSFACLVKLRKKLTHLSVNAIGSIIPKDCVHR
NVIEKCVRLEVLVAIPKVQPRFSVPIWKKYPTMKSFSRVYMNPCKSVHEKEFLLKDWTTEGLLGNDRRLGEICSKRVYRILMAQSGSMRTDDSFHPRRQVEGLECPHLFI
TNATWRQRLVEQRVIVASFIEDLWTLEYRKLKFTFAILILVLRTFAK

CmoCh07G007950 (gene) of Cucurbita moschata (Rifu) v1 genome