CuGenDBv2

Gene ID	CmoCh11G013300
Organism	Cucurbita moschata Rifu (Cucurbita moschata (Rifu) v1)
Description	diphthamide biosynthesis protein 3-like
Genome location	Cmo_Chr11:9193690..9193947
RNA-Seq Expression	CmoCh11G013300
Synteny	CmoCh11G013300
Gene Ontology terms	GO:0002098 - tRNA wobble uridine modification (biological process) GO:0017183 - peptidyl-diphthamide biosynthetic process from peptidyl-histidine (biological process) GO:0005634 - nucleus (cellular component) GO:0005829 - cytosol (cellular component) GO:0046872 - metal ion binding (molecular function)
InterPro domains	IPR007872 - DPH-type metal-binding domain IPR036671 - DPH-type metal-binding domain superfamily IPR044248 - Diphthamide biosynthesis protein 3/4-like

Homology

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GenBank top hits

e value

%identity

Alignment

KAG7022429.1 DPH3, partial [Cucurbita argyrosperma subsp. argyrosperma]

5.0e-43

100

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Query:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
        MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
Subjt:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA

XP_008454889.1 PREDICTED: diphthamide biosynthesis protein 3-like [Cucumis melo]

3.3e-39

92.94

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Query:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
        MSYDDVEIEDM+WNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFL DS QKNN GLEPSKQ P VVA
Subjt:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA

XP_022931585.1 diphthamide biosynthesis protein 3-like [Cucurbita moschata]

5.0e-43

100

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Query:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
        MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
Subjt:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA

XP_022972367.1 diphthamide biosynthesis protein 3-like [Cucurbita maxima]

1.5e-39

92.94

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Query:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
        MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDS  KNN GL+PSKQ+P VVA
Subjt:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA

XP_038887330.1 diphthamide biosynthesis protein 3-like [Benincasa hispida]

1.8e-40

95.29

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Query:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
        MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDS QKNN GLEPSKQ+P VVA
Subjt:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA

TrEMBL top hits

e value

%identity

Alignment

A0A1S3BZL5 diphthamide biosynthesis protein 3-like

1.6e-39

92.94

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Query:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
        MSYDDVEIEDM+WNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFL DS QKNN GLEPSKQ P VVA
Subjt:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA

A0A5A7SJN1 Diphthamide biosynthesis protein 3-like

1.6e-39

92.94

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Query:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
        MSYDDVEIEDM+WNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFL DS QKNN GLEPSKQ P VVA
Subjt:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA

A0A6J1C519 diphthamide biosynthesis protein 3-like

3.6e-39

89.41

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Query:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
        MSYDDVEIEDMEWN+ELQA+TYPCPCGDLFQITKEDL+LGEEIARCPSCSLYITVIYNMEDFLGDS QKNN G++PSKQ+P VVA
Subjt:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA

A0A6J1EZW6 diphthamide biosynthesis protein 3-like

2.4e-43

100

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Query:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
        MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
Subjt:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA

A0A6J1I4L9 diphthamide biosynthesis protein 3-like

7.3e-40

92.94

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Query:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
        MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDS  KNN GL+PSKQ+P VVA
Subjt:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA

SwissProt top hits

e value

%identity

Alignment

Q1LZC9 DPH3 homolog

2.9e-17

52.7

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Query:  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDF-LGDSVQKNNTGLE
        +D+VEIED +++E+ + + YPCPCGD F ITKEDL+ GE++A CPSCSL I VIY+ + F  G++V   +T  E
Subjt:  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDF-LGDSVQKNNTGLE

Q21102 DPH3 homolog

2.2e-17

59.02

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Query:  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFL
        +D+VEIED E++EE   + YPCPCGD F+I +E L++GE++A+CPSCSL I VIY+ EDF+
Subjt:  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFL

Q6CMG4 Diphthamide biosynthesis protein 3

2.2e-17

54.67

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Query:  SYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEP
        +YD+VEIEDM ++ + Q FTYPCPCGD FQI+ +D+  GE+IA CPSCSL I V+++ ED +   V+ N   LEP
Subjt:  SYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEP

Q6VUC1 DPH3 homolog

5.8e-18

52.7

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Query:  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFL-GDSVQKNNTGLE
        +D+VEIED +++E+ + + YPCPCGD F ITKEDL+ GE++A CPSCSL I VIY+ + F+ G++V   +T  E
Subjt:  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFL-GDSVQKNNTGLE

Q8K0W9 DPH3 homolog

7.5e-18

52.7

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Query:  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFL-GDSVQKNNTGLE
        +D+VEIED +++E+ + + YPCPCGD F ITKEDL+ GE++A CPSCSL I VIY+ + F+ G++V   +T  E
Subjt:  YDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFL-GDSVQKNNTGLE

Arabidopsis top hits

e value

%identity

Alignment

AT2G15910.1 CSL zinc finger domain-containing protein

5.9e-34

80.25

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Query:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRP
        MSYDDVEIEDMEWNEE+QA+TYPCPCGDLFQITKEDL+LGEEIA CPSCSLYITVIYNMEDF  D+ +KNN   EP  + P
Subjt:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRP

AT2G15910.2 CSL zinc finger domain-containing protein

5.3e-35

78.82

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Query:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA
        MSYDDVEIEDMEWNEE+QA+TYPCPCGDLFQITKEDL+LGEEIA CPSCSLYITVIYNMEDF  D+ +KNN   EP  + P  VA
Subjt:  MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA

Sequences

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CDS sequence

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ATGTCGTACGATGACGTGGAGATAGAAGACATGGAGTGGAACGAGGAGCTGCAGGCCTTCACGTACCCATGCCCCTGCGGCGACTTGTTCCAAATCACGAAGGAGGATCT
TAAGCTCGGAGAAGAGATTGCTCGGTGCCCTAGCTGCTCTCTTTACATCACTGTCATCTACAACATGGAAGATTTTCTCGGAGACTCTGTTCAGAAGAATAACACAGGAC
TTGAGCCTTCTAAACAACGGCCTGCCGTTGTGGCTTAA

mRNA sequence

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Protein sequence

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MSYDDVEIEDMEWNEELQAFTYPCPCGDLFQITKEDLKLGEEIARCPSCSLYITVIYNMEDFLGDSVQKNNTGLEPSKQRPAVVA

CmoCh11G013300 (gene) of Cucurbita moschata (Rifu) v1 genome