CuGenDBv2

Gene ID	CmoCh14G020210
Organism	Cucurbita moschata Rifu (Cucurbita moschata (Rifu) v1)
Description	EF-hand domain-containing protein
Genome location	Cmo_Chr14:14803750..14806907
RNA-Seq Expression	CmoCh14G020210
Synteny	CmoCh14G020210
Gene Ontology terms	GO:0005509 - calcium ion binding (molecular function) GO:0048306 - calcium-dependent protein binding (molecular function)
InterPro domains	IPR002048 - EF-hand domain IPR011992 - EF-hand domain pair

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6582540.1 hypothetical protein SDJN03_22542, partial [Cucurbita argyrosperma subsp. sororia]

1.4e-83

99.39

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Query:  RMRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSD
        RMRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSD
Subjt:  RMRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSD

Query:  AFISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
        AFISLSHRLILTLVIAPTVAVVTKRSTEGIPV+GSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
Subjt:  AFISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK

KAG7018923.1 hypothetical protein SDJN02_20800, partial [Cucurbita argyrosperma subsp. argyrosperma]

1.1e-72

83.33

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Query:  WDLQSPSRMRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYN--------------DINKRLPISHLDPPTEERFRELIKENDW
        + LQS + +R       GKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLL  +              DINKRLPISHLDPPTEERFRELIKENDW
Subjt:  WDLQSPSRMRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYN--------------DINKRLPISHLDPPTEERFRELIKENDW

Query:  NQDKKIGRDEFLGFILSLTSDAFISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
        NQDKKIGRDEFLGFILSLTSDAFISLSHRLILTLVIAPTVAVVTKRSTEGIPV+GSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
Subjt:  NQDKKIGRDEFLGFILSLTSDAFISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK

XP_022924303.1 uncharacterized protein LOC111431834 [Cucurbita moschata]

4.0e-83

100

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Query:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA
        MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA
Subjt:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA

Query:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
        FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
Subjt:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK

XP_022980436.1 uncharacterized protein LOC111479803 [Cucurbita maxima]

5.8e-82

97.56

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Query:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA
        MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFR+L+KENDWN+DKKIGRDEFLGFILSLTSDA
Subjt:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA

Query:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
        FISLSHRLILTLVIAPTVAVVTKRSTEGIPV+GSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
Subjt:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK

XP_023528258.1 uncharacterized protein LOC111791228 [Cucurbita pepo subsp. pepo]

5.3e-83

99.39

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Query:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA
        MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA
Subjt:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA

Query:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
        FISLSHRLILTLVIAPTVAVVTKRSTEGIPV+GSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
Subjt:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK

TrEMBL top hits

e value

%identity

Alignment

A0A0A0L6I0 EF-hand domain-containing protein

1.7e-47

62.2

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Query:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA
        M +A  R GGKEWR+ QL NIS++IFD  G  K++LSFEDLYIATLLV+NDINK LP  H+DPP ++R +E+++  D NQ+  I R+EFL F+  LTS++
Subjt:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA

Query:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
        FIS+S RLI+TLV+APT+AVVTK+STEG+P +G +VQK+PSSAYA LVTLAA +FQNS++ +LK
Subjt:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK

A0A5A7U485 EF-hand domain-containing protein

3.9e-47

62.8

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Query:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA
        M +AL RFGG++W+++QL  IS++IFD     K+KLSFEDLYIATLLV+NDINK LP  H+DPPT+ER  EL++++D N+++ I RDEFL FI  LT ++
Subjt:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA

Query:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
          S+S RLI+TLV+APT+AVVTKRSTEGIP +G +VQK+PSSAYA LVTLAA +FQNS + +LK
Subjt:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK

A0A6J1D109 uncharacterized protein LOC111016024

2.4e-49

59.89

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Query:  FAWSADFNYSRGLLCEVWDLQSPSRMRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDK--LSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELI
        FA    F+Y R L         PS M + L + GGK+WRD Q+R ISD+IF+ I +  DK  LSFEDLYIATLLVYN+INKRLP  H DPP++ER RELI
Subjt:  FAWSADFNYSRGLLCEVWDLQSPSRMRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDK--LSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELI

Query:  KENDWNQDKKIGRDEFLGFILSLTSDAFISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQK
        KE+D N D++I R+EF+ FIL LTSD  I++S  L+LTLV+APTVAVVTK+STEG+P +G LVQKLP+S YA L+TLAA  FQN+ +
Subjt:  KENDWNQDKKIGRDEFLGFILSLTSDAFISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQK

A0A6J1EC16 uncharacterized protein LOC111431834

2.0e-83

100

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Query:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA
        MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA
Subjt:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA

Query:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
        FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
Subjt:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK

A0A6J1ITL1 uncharacterized protein LOC111479803

2.8e-82

97.56

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Query:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA
        MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFR+L+KENDWN+DKKIGRDEFLGFILSLTSDA
Subjt:  MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDA

Query:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
        FISLSHRLILTLVIAPTVAVVTKRSTEGIPV+GSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK
Subjt:  FISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G64850.1 Calcium-binding EF hand family protein

4.4e-35

48.37

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Query:  MRKALGRFGGKEWRDEQLRNISDRIFD--MIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTS
        M +   +  GKEWR +Q++ I DR+FD   +   +  L+FE+LYIA LLVYNDINKRLP  H DPP+++    ++ + D N D ++ R+EF+ FI  +T+
Subjt:  MRKALGRFGGKEWRDEQLRNISDRIFD--MIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTS

Query:  DAFISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLA
        + F  +S  LI+ L++APTVA+ TK++TEG+P +G +VQKLP+S YA LVTLA
Subjt:  DAFISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLA

AT4G37445.1 BEST Arabidopsis thaliana protein match is: Calcium-binding EF hand family protein (TAIR:AT1G64850.1)

4.7e-05

24.58

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Query:  SFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDAFISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLV
        +F+D ++A L ++N+ N  LP  H D PT ++ +         +D++  +  F+ F+      +   L    ++T +++P  A+  KR+ E +P +  L+
Subjt:  SFEDLYIATLLVYNDINKRLPISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDAFISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLV

Query:  QKLPSSAYAFLVTLAAFV
        + +P   +   VT+ A V
Subjt:  QKLPSSAYAFLVTLAAFV

Sequences

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CDS sequence

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ATGATAACTTGCAGCCGAGGGAAGCGAAGATCTTCATCTTCCTTTGCCTGGAGCGCCGATTTCAATTACAGTCGGGGGCTGCTTTGCGAGGTCTGGGATCTTCAATCGCC
GTCTAGAATGAGAAAGGCCTTGGGGAGATTTGGAGGCAAAGAATGGAGGGACGAGCAATTGAGGAATATATCAGATAGAATATTTGATATGATTGGAGATAAGAAGGATA
AGCTGTCGTTTGAAGATCTGTACATTGCTACGTTACTTGTATACAATGATATTAACAAGCGGTTGCCTATCTCTCATCTTGATCCGCCCACAGAGGAACGTTTCAGAGAG
CTCATAAAGGAGAACGACTGGAACCAGGATAAAAAAATTGGGCGTGACGAATTCTTGGGTTTTATACTGAGCTTGACATCTGACGCGTTCATATCACTGAGCCACAGATT
GATCCTCACTTTGGTGATAGCACCAACAGTCGCAGTGGTCACAAAGAGAAGTACTGAAGGCATCCCAGTCATTGGAAGCCTGGTCCAAAAACTGCCCTCTTCAGCTTATG
CTTTCCTCGTCACCCTTGCTGCTTTCGTGTTTCAAAACTCTCAGAAGCCGATGCTTAAATGA

mRNA sequence

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Protein sequence

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MITCSRGKRRSSSSFAWSADFNYSRGLLCEVWDLQSPSRMRKALGRFGGKEWRDEQLRNISDRIFDMIGDKKDKLSFEDLYIATLLVYNDINKRLPISHLDPPTEERFRE
LIKENDWNQDKKIGRDEFLGFILSLTSDAFISLSHRLILTLVIAPTVAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK

CmoCh14G020210 (gene) of Cucurbita moschata (Rifu) v1 genome