CuGenDBv2

Gene ID	CmoCh15G002340
Organism	Cucurbita moschata Rifu (Cucurbita moschata (Rifu) v1)
Description	tRNA (guanine(26)-N(2))-dimethyltransferase
Genome location	Cmo_Chr15:1159630..1161110
RNA-Seq Expression	CmoCh15G002340
Synteny	CmoCh15G002340
Gene Ontology terms	GO:0002940 - tRNA N2-guanine methylation (biological process) GO:0005634 - nucleus (cellular component) GO:0003723 - RNA binding (molecular function) GO:0004809 - tRNA (guanine-N2-)-methyltransferase activity (molecular function)
InterPro domains	IPR002905 - tRNA methyltransferase, Trm1

Homology

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GenBank top hits

e value

%identity

Alignment

KAG6578537.1 putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia]

4.1e-41

97.78

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Query:  KNHPVKAQPEDQPGIIILAKVLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDESAPNQ
        KNHPVKA+PEDQPGIIILAKVLKAQARKVARFLPNPERHWGSKLR GRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDESAPNQ
Subjt:  KNHPVKAQPEDQPGIIILAKVLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDESAPNQ

KAG7016097.1 putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyrosperma subsp. argyrosperma]

9.2e-41

96.67

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Query:  KNHPVKAQPEDQPGIIILAKVLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDESAPNQ
        KNHPVKA+PEDQPGIIILAKVLKAQARK ARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYE+PKAQRPKVEDESAPNQ
Subjt:  KNHPVKAQPEDQPGIIILAKVLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDESAPNQ

XP_022939541.1 probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 isoform X1 [Cucurbita moschata]

4.3e-46

97.98

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Query:  MGHFLPGAKNHPVKAQPEDQPGIIILAKVL-KAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDESAPNQ
        MGHFLPGAKNHPVKAQPEDQPGIIILAKVL +AQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDESAPNQ
Subjt:  MGHFLPGAKNHPVKAQPEDQPGIIILAKVL-KAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDESAPNQ

XP_022939542.1 probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 isoform X2 [Cucurbita moschata]

4.1e-41

97.78

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Query:  MGHFLPGAKNHPVKAQPEDQPGIIILAKVL-KAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPK
        MGHFLPGAKNHPVKAQPEDQPGIIILAKVL +AQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPK
Subjt:  MGHFLPGAKNHPVKAQPEDQPGIIILAKVL-KAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPK

XP_038890035.1 probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida]

1.9e-30

71.15

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Query:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDES
        KNHPVKAQPEDQPG IILAK              + KAQA+K+ARFLPNPERHWG KLRAGRQITS +ISLL AEA+ GALSHTD EEP A+RPKVE+E 
Subjt:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDES

Query:  APNQ
        APNQ
Subjt:  APNQ

TrEMBL top hits

e value

%identity

Alignment

A0A1S3CA23 tRNA (guanine(26)-N(2))-dimethyltransferase

9.3e-31

71.15

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Query:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDES
        KNHPVKAQPEDQPG IILAK              + KAQA+K+ARFLPNPERHWG KLRAGRQITS +ISLL AEA+ GALSHTD EEP A+RPKVE+E 
Subjt:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDES

Query:  APNQ
        APNQ
Subjt:  APNQ

A0A5A7T7H3 tRNA (guanine(26)-N(2))-dimethyltransferase

9.3e-31

71.15

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Query:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDES
        KNHPVKAQPEDQPG IILAK              + KAQA+K+ARFLPNPERHWG KLRAGRQITS +ISLL AEA+ GALSHTD EEP A+RPKVE+E 
Subjt:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDES

Query:  APNQ
        APNQ
Subjt:  APNQ

A0A6J1FG75 probable tRNA (Guanine(26)-N(2))-dimethyltransferase 2 isoform X1

2.1e-46

97.98

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Query:  MGHFLPGAKNHPVKAQPEDQPGIIILAKVL-KAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDESAPNQ
        MGHFLPGAKNHPVKAQPEDQPGIIILAKVL +AQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDESAPNQ
Subjt:  MGHFLPGAKNHPVKAQPEDQPGIIILAKVL-KAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDESAPNQ

A0A6J1FH33 probable tRNA (Guanine(26)-N(2))-dimethyltransferase 2 isoform X2

2.0e-41

97.78

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Query:  MGHFLPGAKNHPVKAQPEDQPGIIILAKVL-KAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPK
        MGHFLPGAKNHPVKAQPEDQPGIIILAKVL +AQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPK
Subjt:  MGHFLPGAKNHPVKAQPEDQPGIIILAKVL-KAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPK

A0A6J1JNF2 tRNA (guanine(26)-N(2))-dimethyltransferase

5.1e-29

69.23

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Query:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDES
        KNHPVKAQPEDQPG IILAK              + KAQA+KVARFLPNPERHWG KLRAGRQITS +ISLL AEA+ GALSH D EEP A+R KVE++ 
Subjt:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDES

Query:  APNQ
        APNQ
Subjt:  APNQ

SwissProt top hits

e value

%identity

Alignment

Q9LFU5 Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1

4.3e-17

46.46

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Query:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDE
        KNHP+K Q  + PG +IL+K              + KAQA+KVARFLPNPE+HWG K+RAGR ITS ++SLL  EA+ G L++   E    +  + E+E
Subjt:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDE

Q9SRU7 Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2

3.5e-19

52.53

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Query:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDE
        KNHP+KAQ  +QPG +IL+K              + KAQA+KVARFLPNPE+HWG KLRAGRQITS ++SL+  EA+ G LS   +EE K +  + E E
Subjt:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDE

Arabidopsis top hits

e value

%identity

Alignment

AT3G02320.1 N2,N2-dimethylguanosine tRNA methyltransferase

2.5e-20

52.53

Show/hide

Query:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDE
        KNHP+KAQ  +QPG +IL+K              + KAQA+KVARFLPNPE+HWG KLRAGRQITS ++SL+  EA+ G LS   +EE K +  + E E
Subjt:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDE

AT5G15810.1 N2,N2-dimethylguanosine tRNA methyltransferase

3.1e-18

46.46

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Query:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDE
        KNHP+K Q  + PG +IL+K              + KAQA+KVARFLPNPE+HWG K+RAGR ITS ++SLL  EA+ G L++   E    +  + E+E
Subjt:  KNHPVKAQPEDQPGIIILAK--------------VLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDE

Sequences

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CDS sequence

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ATGGGTCACTTTTTACCTGGGGCAAAAAACCACCCAGTAAAAGCTCAACCAGAAGATCAGCCAGGAATTATTATTCTTGCCAAGGTCTTGAAGGCACAAGCAAGG
AAAGTTGCACGCTTCCTTCCAAACCCTGAAAGACATTGGGGCTCCAAGCTGAGAGCTGGTCGTCAAATCACTAGTGTTTACATCTCTCTATTGGATGCAGAGGCA
ATAATTGGAGCTCTTAGCCATACGGATTACGAAGAGCCTAAAGCACAACGTCCCAAGGTAGAGGATGAATCAGCTCCCAACCAGTAA

mRNA sequence

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CCCCTATGAAGCGAGGGATTGCTTATATTATTCTGCAAGTCGTCTTCGTTTTCAATATCTGTCTGTATGCTTCATTTGGTGGAAATTTTCGAATGCTTTAGGGCC
TAGAGATGGACATTATGGATGCAATAGCTTGGGGCCGTGAACATACGGTAGCTGTGGCGTCTGATGGGTCACTTTTTACCTGGGGCAAAAAACCACCCAGTAAAA
GCTCAACCAGAAGATCAGCCAGGAATTATTATTCTTGCCAAGGTCTTGAAGGCACAAGCAAGGAAAGTTGCACGCTTCCTTCCAAACCCTGAAAGACATTGGGGC
TCCAAGCTGAGAGCTGGTCGTCAAATCACTAGTGTTTACATCTCTCTATTGGATGCAGAGGCAATAATTGGAGCTCTTAGCCATACGGATTACGAAGAGCCTAAA
GCACAACGTCCCAAGGTAGAGGATGAATCAGCTCCCAACCAGTAAAAGTTTCCTTATCTTAAATTTTCTATTTCTGAGTTATGCAGTATCTACTTTTTGACCCTC
CGCATGAAATGCTCGTTTGGGGACGCTTAGGTAGAAGTAGCAGATTTTTGTTTAATTTTGAGGAAGGCTTTCTTTCAAAATATATCATTTTTGTTCATTTCTAGG
AGGTTTAGATTCCTGTCGAGAGAGAGAGAGATCAAGATTTACAGGGAATGCACAAGAAGTCTTTCCCTCTCCATCCCTATGATCAGCAGATGTTTCTGAACGCAA
GCTGGTGGTGGTTGTTCTTATTTCCTCTCCAACGGAGGGTTTTTTATTTTTATTTTTAATTTTTGAGCTGTCTTCGTACAAAGTCAAGTTCAATAGGAATACTAA
GGTGTTGTATGGATTTAATACCTCTTAGTTTTTAGAGCTTAGGATTGGCAAATTCAGATTGGTGCAACTTATATCTGGTTATACAAAGGAACGAGTTATACGAAC
TGTGAGCTAAGTCCCAAC

Protein sequence

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MGHFLPGAKNHPVKAQPEDQPGIIILAKVLKAQARKVARFLPNPERHWGSKLRAGRQITSVYISLLDAEAIIGALSHTDYEEPKAQRPKVEDESAPNQ

CmoCh15G002340 (gene) of Cucurbita moschata (Rifu) v1 genome