CuGenDBv2

Gene ID	CsGy4G011923
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	tRNA (Guanine(10)-N2)-methyltransferase homolog
Genome location	Gy14Chr4:12177326..12179391
RNA-Seq Expression	CsGy4G011923
Synteny	CsGy4G011923
Gene Ontology terms	GO:0001510 - RNA methylation (biological process) GO:0034470 - ncRNA processing (biological process) GO:0003676 - nucleic acid binding (molecular function) GO:0008168 - methyltransferase activity (molecular function)
InterPro domains	IPR000241 - Putative RNA methylase domain IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase

Homology

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GenBank top hits

e value

%identity

Alignment

KAE8637124.1 hypothetical protein CSA_021500 [Cucumis sativus]

6.37e-136

100

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH
        FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH
Subjt:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH

KAE8637127.1 hypothetical protein CSA_021503 [Cucumis sativus]

4.86e-135

100

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH
        FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH
Subjt:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH

KGN44172.2 hypothetical protein Csa_018210 [Cucumis sativus]

1.98e-132

98.92

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFG  VGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH
        FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH
Subjt:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH

TYK17862.1 tRNA (guanine(10)-N2)-methyltransferase-like protein [Cucumis melo var. makuwa]

5.39e-110

99.36

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV
        FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKE+
Subjt:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV

XP_031745590.1 tRNA (guanine(10)-N2)-methyltransferase homolog isoform X6 [Cucumis sativus]

6.54e-134

100

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH
        FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH
Subjt:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH

TrEMBL top hits

e value

%identity

Alignment

A0A1S3BG98 tRNA (Guanine(10)-N2)-methyltransferase homolog

2.40e-108

99.36

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV
        FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKE+
Subjt:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV

A0A5A7TF32 tRNA (Guanine(10)-N2)-methyltransferase-like protein

4.17e-107

99.36

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV
        FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKE+
Subjt:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV

A0A5D3D1Y2 tRNA (Guanine(10)-N2)-methyltransferase-like protein

2.61e-110

99.36

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV
        FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKE+
Subjt:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV

A0A6J1D5Q5 tRNA (Guanine(10)-N2)-methyltransferase homolog

9.91e-103

94.27

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        GQVNLK+PDHKFCL+E ADYGLNNGLPPIAQR IFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALAT+GKLV+DPFVGTGSILV AAH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV
        FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLP PIALLRADNNLPPWRPGLKE+
Subjt:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV

A0A6J1ICG5 tRNA (Guanine(10)-N2)-methyltransferase homolog

1.32e-102

93.63

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        GQVNL++PDHKF L+E ADYGLNNGLPP+AQR IFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALAT GKLV+DPFVGTGSILVGAAH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV
        FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKE+
Subjt:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV

SwissProt top hits

e value

%identity

Alignment

Q05B63 tRNA (guanine(10)-N2)-methyltransferase homolog

5.9e-31

42.68

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        G+VNLK P H F +LE  DYGL+    P     I+FGR +    R+LI +Y +K R ++G T+MDA ++F+MAN        +VFDPFVGTG +L+ +AH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPG
        FGA   G DID   V          +  RGPD N+ +N +QYGL    + +L +D + P WR G
Subjt:  FGAMTMGADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPG

Q5R962 tRNA (guanine(10)-N2)-methyltransferase homolog

5.9e-31

42.68

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        G+VNLK P H F +LE  DYGL+    P     I+FGR +    R+LI +Y +K R ++G T+MDA ++F+MAN        +VFDPFVGTG +L+  AH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPG
        FGA   G DID   V          +  RGPD N+ +N +QYGL    + +L +D + P WR G
Subjt:  FGAMTMGADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPG

Q7TNK6 tRNA (guanine(10)-N2)-methyltransferase homolog

6.9e-32

43.9

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        G+VNLK P H F +LE  DYGL+    P     I+FGR +    R+LI +Y +K R ++G T+MDA ++F+MAN A      LVFDPFVGTG +L+ +AH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPG
        FGA   G DID   V          +  RGPD N+ +N +QYGL    + +L +D + P WR G
Subjt:  FGAMTMGADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPG

Q7Z4G4 tRNA (guanine(10)-N2)-methyltransferase homolog

5.9e-31

42.68

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        G+VNLK P H F +LE  DYGL+    P     I+FGR +    R+LI +Y +K R ++G T+MDA ++F+MAN        +VFDPFVGTG +L+  AH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPG
        FGA   G DID   V          +  RGPD N+ +N +QYGL    + +L +D + P WR G
Subjt:  FGAMTMGADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPG

Q9CWH5 tRNA (guanine(10)-N2)-methyltransferase homolog

2.6e-31

43.29

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        G+VNLK P H F +LE  DYGL+    P     I+FGR +    R+LI +Y +K R ++G T+MDA ++F+M N A      LVFDPFVGTG +L+ +AH
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPG
        FGA   G DID   V          +  RGPD N+ +N +QYGL    + +L +D + P WR G
Subjt:  FGAMTMGADIDIRVV----------RDGRGPDCNVWSNFKQYGLPP-PIALLRADNNLPPWRPG

Arabidopsis top hits

e value

%identity

Alignment

AT3G26410.1 methyltransferases;nucleic acid binding

6.8e-75

83.44

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Query:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH
        G+VNLK+PDH F L+E  +   NNGL PI QRRIFFGREVG ADRKL+PT+QLKSRTYLGPTAMDAEMAFLMANQA ATSGKLV+DPFVGTGSILV AA 
Subjt:  GQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAH

Query:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV
        FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLP P+ALLR DNN+PPWR GLKE+
Subjt:  FGAMTMGADIDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEV

Sequences

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CDS sequence

Show/hide CDS sequence

ATGGGTCAAGTTAATTTAAAAAGTCCAGATCATAAATTTTGTCTTTTGGAAACTGCTGATTACGGACTCAATAATGGACTTCCACCAATTGCTCAAAGGAGAATTTTTTT
TGGCCGGGAGGTCGGTGGTGCCGATAGGAAGCTTATACCAACATATCAATTAAAAAGTCGCACTTATCTTGGGCCAACTGCCATGGATGCTGAAATGGCTTTCTTAATGG
CCAACCAAGCATTAGCGACATCTGGAAAACTTGTCTTTGATCCTTTTGTTGGCACTGGGAGCATTCTTGTTGGCGCAGCTCATTTTGGAGCAATGACAATGGGGGCAGAC
ATTGACATTAGGGTGGTACGTGATGGTCGTGGCCCTGATTGTAATGTTTGGAGCAACTTCAAGCAGTATGGATTACCACCTCCAATTGCACTGTTAAGAGCAGATAATAA
TCTTCCTCCCTGGCGTCCAGGATTGAAGGAGGTACAGCAGCAATATTTGCACCAGTACTATTTATTTATTTTTCGTGGTGCTGAAGTTTCACTACCCTTGTTATATACTG
ATGGTCACTAA

mRNA sequence

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Protein sequence

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MGQVNLKSPDHKFCLLETADYGLNNGLPPIAQRRIFFGREVGGADRKLIPTYQLKSRTYLGPTAMDAEMAFLMANQALATSGKLVFDPFVGTGSILVGAAHFGAMTMGAD
IDIRVVRDGRGPDCNVWSNFKQYGLPPPIALLRADNNLPPWRPGLKEVQQQYLHQYYLFIFRGAEVSLPLLYTDGH

CsGy4G011923 (gene) of Cucumber (Gy14) v2.1 genome