CuGenDBv2

Gene ID	Cucsat.G10077
Organism	Cucumis sativus L. var. sativus cv. B10 (Cucumber (B10) v3)
Description	mediator of RNA polymerase II transcription subunit 23
Genome location	ctg1673:4536065..4541886
RNA-Seq Expression	Cucsat.G10077
Synteny	Cucsat.G10077
Gene Ontology terms	GO:0006357 - regulation of transcription by RNA polymerase II (biological process) GO:0010628 - positive regulation of gene expression (biological process) GO:0005634 - nucleus (cellular component) GO:0005667 - transcription factor complex (cellular component) GO:0016592 - mediator complex (cellular component)
InterPro domains	IPR021629 - Mediator complex, subunit Med23

Homology

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GenBank top hits

e value

%identity

Alignment

KYP63340.1 Mediator of RNA polymerase II transcription subunit 23 [Cajanus cajan]

7.18e-78

90.15

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLC KL+YQME++LFSGQ +PSIA+ ETYTRLLLLAPHSLFRSHFNHL QRNPS LS+PGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISA+KGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHRVFRLAENLC+NLIFSLRDFFFVKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

XP_008467111.1 PREDICTED: mediator of RNA polymerase II transcription subunit 23 isoform X1 [Cucumis melo]

1.42e-77

100

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

XP_011655454.1 mediator of RNA polymerase II transcription subunit 23 [Cucumis sativus]

1.42e-77

100

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

XP_016898808.1 PREDICTED: mediator of RNA polymerase II transcription subunit 23 isoform X2 [Cucumis melo]

1.41e-77

100

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

XP_038875594.1 mediator of RNA polymerase II transcription subunit 23 [Benincasa hispida]

3.61e-77

99.24

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLS+PGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KPD2 Uncharacterized protein

4.80e-79

100

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

A0A151T8F0 Mediator of RNA polymerase II transcription subunit 23

3.48e-78

90.15

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLC KL+YQME++LFSGQ +PSIA+ ETYTRLLLLAPHSLFRSHFNHL QRNPS LS+PGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISA+KGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHRVFRLAENLC+NLIFSLRDFFFVKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

A0A1S3CSR7 mediator of RNA polymerase II transcription subunit 23 isoform X1

6.88e-78

100

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

A0A1S4DS63 mediator of RNA polymerase II transcription subunit 23 isoform X2

6.81e-78

100

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

A0A5A7TSF1 Mediator of RNA polymerase II transcription subunit 23 isoform X1

6.88e-78

100

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

SwissProt top hits

e value

%identity

Alignment

F4I4P3 Mediator of RNA polymerase II transcription subunit 23

6.1e-59

85.61

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLC KLA QMEE+LF  Q VPSIA+ ETYTRLLL++PHS+FRSHF+ LAQRN SLLS+PGVTLLVLEILNYRLLPLYRYQGKSK LMYDVTKIISALKGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHR+FRLAENLCMNLI SLRDFF VKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

Arabidopsis top hits

e value

%identity

Alignment

AT1G23230.1 CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med23 (InterPro:IPR021629); Has 187 Blast hits to 184 proteins in 67 species: Archae - 0; Bacteria - 0; Metazoa - 135; Fungi - 0; Plants - 43; Viruses - 0; Other Eukaryotes - 9 (source: NCBI BLink).

4.4e-60

85.61

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLC KLA QMEE+LF  Q VPSIA+ ETYTRLLL++PHS+FRSHF+ LAQRN SLLS+PGVTLLVLEILNYRLLPLYRYQGKSK LMYDVTKIISALKGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHR+FRLAENLCMNLI SLRDFF VKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

AT1G23230.2 FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to salt stress; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Mediator complex subunit Med23 (InterPro:IPR021629).

7.7e-57

83.33

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Query:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK
        NLC KLA QMEE+LF  Q VPSIA+ ETYTRLLL++PHS+FRSHF+    RN SLLS+PGVTLLVLEILNYRLLPLYRYQGKSK LMYDVTKIISALKGK
Subjt:  NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGK

Query:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK
        RGDHR+FRLAENLCMNLI SLRDFF VKREGK
Subjt:  RGDHRVFRLAENLCMNLIFSLRDFFFVKREGK

Sequences

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CDS sequence

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AACTTGTGCTCGAAGCTGGCATATCAAATGGAGGAATCCTTATTTTCTGGACAGGCTGTTCCCAGTATTGCATTGGCTGAAACATATACTAGGTTGCTTCTCCTTGCACC
ACACTCATTGTTTCGCTCGCATTTTAATCATTTAGCACAAAGAAACCCATCCCTATTGAGCAGACCAGGAGTTACGCTTCTAGTGCTTGAAATTTTGAACTATCGGTTGC
TTCCTCTATACAGGTACCAAGGCAAGAGTAAGGCTTTGATGTATGATGTGACAAAAATAATCTCTGCACTCAAAGGAAAGCGTGGAGACCATCGAGTATTTAGATTGGCT
GAAAACTTGTGCATGAATCTTATTTTTTCATTAAGAGATTTTTTCTTTGTGAAAAGGGAAGGGAAG

mRNA sequence

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Protein sequence

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NLCSKLAYQMEESLFSGQAVPSIALAETYTRLLLLAPHSLFRSHFNHLAQRNPSLLSRPGVTLLVLEILNYRLLPLYRYQGKSKALMYDVTKIISALKGKRGDHRVFRLA
ENLCMNLIFSLRDFFFVKREGK

Cucsat.G10077 (gene) of Cucumber (B10) v3 genome