CuGenDBv2

Gene ID	Cucsat.G12374
Organism	Cucumis sativus L. var. sativus cv. B10 (Cucumber (B10) v3)
Description	phosphatidyl-N-methylethanolamine N-methyltransferase
Genome location	ctg1837:1435314..1438963
RNA-Seq Expression	Cucsat.G12374
Synteny	Cucsat.G12374
Gene Ontology terms	GO:0006656 - phosphatidylcholine biosynthetic process (biological process) GO:0032259 - methylation (biological process) GO:0005789 - endoplasmic reticulum membrane (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0000773 - phosphatidyl-N-methylethanolamine N-methyltransferase activity (molecular function) GO:0004608 - phosphatidylethanolamine N-methyltransferase activity (molecular function) GO:0080101 - phosphatidyl-N-dimethylethanolamine N-methyltransferase activity (molecular function)
InterPro domains	IPR007318 - Phospholipid methyltransferase IPR024960 - Phosphatidyl-N-methylethanolamine/Phosphatidylethanolamine N-methyltransferase

Homology

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GenBank top hits

e value

%identity

Alignment

KAG7036543.1 Phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma]

1.58e-109

90.85

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Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        M + AVIGVLLPFPYYYVLW RPQLWVNLCGK  EP KLMAQVSVFLKLLQFLSI+HVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS
        NIPWVTEFPFGYIKDPQYVGSILSL ACLSWVPL+YILLWT+GY+FMM VESKEDL TRAKPQS
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS

XP_004137657.1 phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis sativus]

5.77e-121

100

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Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS
        NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS

XP_008465151.2 PREDICTED: phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis melo]

5.55e-119

98.78

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Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGI AVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS
        NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKED ATRAKPQS
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS

XP_023523751.1 phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita pepo subsp. pepo]

4.52e-109

90.24

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Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        M + AVIGVLLPFPYYYVLW RPQLWVNLCGK  EP KLMAQVSVFLKLLQFLSI+HVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS
        NIPWVTEFPFGYIKDPQYVGSILSL ACLSWVPL+YILLWT+GY+FMM VESKEDL TRAKPQ+
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS

XP_038903463.1 phosphatidyl-N-methylethanolamine N-methyltransferase [Benincasa hispida]

8.48e-114

92.68

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Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGI AV+GVLLPFPYYYVLW+RPQLWVNLCGKSR+PCKLMAQVSVFLKLLQFLSI HVSSF+WPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS
        NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPL+YI+LWTLGYIFMMH+ESKE  ATRAKPQS
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS

TrEMBL top hits

e value

%identity

Alignment

A0A1S3CPQ5 phosphatidyl-N-methylethanolamine N-methyltransferase

2.68e-119

98.78

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Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGI AVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS
        NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKED ATRAKPQS
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS

A0A2P6RVH2 Phosphatidyl-N-methylethanolamine N-methyltransferase

4.16e-98

80.49

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Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGI A IGVL PFP+YY LW  PQ WV LCG  R+PCK+MA V+ FLKL+QFLS++ VS+ SWPPPLYFWPL+AFGQFLNFRVYQLLGE+G YYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS
        NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVP  YI LWTLGY+FM+HVESKED ATRAKP S
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS

A0A6J1DHC8 phosphatidyl-N-methylethanolamine N-methyltransferase

5.15e-108

89.02

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Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MGI A IGVLLPFP+YYVLW RPQLW N CGK REPCKLMA+VS+FLKLLQFLSI+HVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS
        NIPWVTEFPFGYI+DPQYVGSILS+FACLSWVPL+YILL TLGY+FMM VESKED  TRAKPQS
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS

A0A6J1GCG7 phosphatidyl-N-methylethanolamine N-methyltransferase

6.27e-109

90.24

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Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        M + AVIGVLLPFPYYYVLW RPQLWVNLCGK  EP KLMAQVSVFLKLLQ LSI+HVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS
        NIPWVTEFPFGYIKDPQYVGSILSL ACLSWVPL+YILLWT+GY+FMM VESKEDL TRAKPQS
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS

A0A6J1KB80 phosphatidyl-N-methylethanolamine N-methyltransferase

2.19e-109

90.24

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Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        M + AVIGVLLPFPYYY+LW RPQLWVNLCGK  EP KLMAQVSVFLKLLQFLSI+HVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS
        NIPWVTEFPFGYIKDPQYVGSILSL ACLSWVPL+YILLWT+GY+FMM VESKEDL TRAKPQS
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS

SwissProt top hits

e value

%identity

Alignment

Q9SAH5 Phosphatidyl-N-methylethanolamine N-methyltransferase

1.2e-68

73.91

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Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MG+ A IGVLLPFP+Y+ LW   Q WVNLCG+ R+P  +MA+VS  LK  Q LS+  V+S SWPPPLYFWPLMAFGQFLNFRVYQLLGE+G YYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAK
        NIPWVTEFPFG I+DPQYVGSI+SL ACLSWVP +YILLW+LGY+FMM +ESKED   RAK
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAK

Arabidopsis top hits

e value

%identity

Alignment

AT1G80860.1 phospholipid N-methyltransferase

8.6e-70

73.91

Show/hide

Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MG+ A IGVLLPFP+Y+ LW   Q WVNLCG+ R+P  +MA+VS  LK  Q LS+  V+S SWPPPLYFWPLMAFGQFLNFRVYQLLGE+G YYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAK
        NIPWVTEFPFG I+DPQYVGSI+SL ACLSWVP +YILLW+LGY+FMM +ESKED   RAK
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAK

AT1G80860.2 phospholipid N-methyltransferase

8.6e-70

73.91

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Query:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK
        MG+ A IGVLLPFP+Y+ LW   Q WVNLCG+ R+P  +MA+VS  LK  Q LS+  V+S SWPPPLYFWPLMAFGQFLNFRVYQLLGE+G YYGVRFGK
Subjt:  MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGK

Query:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAK
        NIPWVTEFPFG I+DPQYVGSI+SL ACLSWVP +YILLW+LGY+FMM +ESKED   RAK
Subjt:  NIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAK

Sequences

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CDS sequence

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CTCCTCTTCTTCCTTCGGTATCATTTTCCCGAGAAAGTGGAGCGAAAATCCGGCCAGAAAATTATCAGAATGGGCATTTTCGCCGTAATTGGAGTGTTGTTGCCATTTCC
GTACTACTACGTCTTATGGAATCGTCCTCAGTTGTGGGTGAACTTGTGCGGCAAATCGCGCGAACCCTGCAAGTTGATGGCCCAGGTCTCGGTTTTTCTCAAGTTGCTTC
AGTTCCTCTCCATCATTCATGTCTCCTCCTTCTCATGGCCCCCGCCTCTATACTTCTGGCCTCTAATGGCTTTCGGACAGTTCTTGAACTTCAGGGTCTACCAGTTACTC
GGTGAATCTGGTGTTTACTATGGAGTACGATTTGGGAAGAACATTCCATGGGTAACTGAATTTCCATTTGGATATATAAAAGATCCTCAATATGTGGGAAGCATTTTGAG
CCTTTTTGCTTGTCTATCGTGGGTACCTTTAAAATATATTCTGCTCTGGACACTGGGTTACATTTTTATGATGCATGTTGAATCCAAGGAAGATCTTGCAACTCGAGCAA
AGCCTCAAAGTTGA

mRNA sequence

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Protein sequence

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LLFFLRYHFPEKVERKSGQKIIRMGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLL
GESGVYYGVRFGKNIPWVTEFPFGYIKDPQYVGSILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS

Cucsat.G12374 (gene) of Cucumber (B10) v3 genome