CuGenDBv2

Gene ID	Cucsat.G14554
Organism	Cucumis sativus L. var. sativus cv. B10 (Cucumber (B10) v3)
Description	protein MIS12 homolog
Genome location	ctg1869:1093280..1095708
RNA-Seq Expression	Cucsat.G14554
Synteny	Cucsat.G14554
Gene Ontology terms	GO:0000070 - mitotic sister chromatid segregation (biological process) GO:0034501 - protein localization to kinetochore (biological process) GO:0051301 - cell division (biological process) GO:0051382 - kinetochore assembly (biological process) GO:0000818 - nuclear MIS12/MIND complex (cellular component)
InterPro domains	IPR008685 - Centromere protein Mis12

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAA0058004.1 Centromere protein Mis12 [Cucumis melo var. makuwa]

4.48e-151

94.56

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Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD
        MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTLGISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKR-TEET
        DESPGVTSI+HD DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+TASELR KIGK+KKR TEE 
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKR-TEET

Query:  KLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Subjt:  KLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

KAA0058032.1 Centromere protein Mis12 [Cucumis melo var. makuwa]

3.68e-150

94.14

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Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD
        MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTE SDRSQDLTLGISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKR-TEET
        DESPGVTSI+HD DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+TASELR KIGK+KKR TEE 
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKR-TEET

Query:  KLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Subjt:  KLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

XP_004138204.3 protein MIS12 homolog isoform X1 [Cucumis sativus]

3.22e-162

100

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Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD
        MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRTEETK
        DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRTEETK
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRTEETK

Query:  LAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        LAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
Subjt:  LAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

XP_023515499.1 protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]

2.77e-134

84.23

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Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTE SDRSQDL+LGIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPS+
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD

Query:  DESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRT-E
        DESP VTS+S D  +DVDLDTELD LRNKLSEVRKEN+VLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MFQEM+ TASELR KIGKLKKR  E
Subjt:  DESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRT-E

Query:  ETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        E+KL  VEK+HTNGDISHHHKGFSNAKLDDIQEFL+DLK +
Subjt:  ETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

XP_038879945.1 protein MIS12 homolog [Benincasa hispida]

5.52e-136

86.19

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Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAF FYQSQASA LKTE SDRSQDLTLGIS+VRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRT-EET
        D+SPGVTSISHD+DV LDTELD LRNKLSEVRKENIVLNQELQALERQTA+SNSQI+HFNEALQLYEQ+SVN+MFQEM+ TASELR KIGKLKKR  EE+
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRT-EET

Query:  KLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        KL  VEKVHTNGDISH+H G SNAKLDDI+ FL DLK +
Subjt:  KLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LPL2 Uncharacterized protein

1.82e-161

99.58

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Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD
        MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRTEETK
        DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRTEETK
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRTEETK

Query:  LAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        LAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLS LKKI
Subjt:  LAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

A0A5A7UQ95 Centromere protein Mis12

1.78e-150

94.14

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Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD
        MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASA LKTE SDRSQDLTLGISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKR-TEET
        DESPGVTSI+HD DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+TASELR KIGK+KKR TEE 
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKR-TEET

Query:  KLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Subjt:  KLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

A0A5A7US57 Centromere protein Mis12

2.17e-151

94.56

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Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD
        MEGSKGE VFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTE SDRSQDLTLGISQVRALVQLGL KRLAMWEKYCLNHCFSVPEGFSLPS+
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD

Query:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKR-TEET
        DESPGVTSI+HD DVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMI+TASELR KIGK+KKR TEE 
Subjt:  DESPGVTSISHDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKR-TEET

Query:  KLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        KLA VEKVHTNGDISHHH GFSNAKLDDIQEFLSDLKKI
Subjt:  KLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

A0A6J1E0A1 protein MIS12 homolog

2.59e-132

83.4

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Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTE SDRSQDL LGIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPS+
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD

Query:  DESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRT-E
        DESP VTS+S D  +DVDLDTELD LRNKLSEVRKENIVLNQELQALERQ ASSNSQ++ FNEALQLYE++SVN+MFQEM+ TASELR KIGKLKKR  E
Subjt:  DESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRT-E

Query:  ETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        E+KL  VEK+HTNGD SHHHKGFSNAKLDDIQEFL+DLK +
Subjt:  ETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

A0A6J1JME3 protein MIS12 homolog isoform X2

1.49e-131

83.4

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD
        MEGSKGE VF+SLNLNPQLFINEALN VDDLVDDAFDFYQ+QASA+LKTE SDRSQDL LGIS+VR  VQ GL KRLAMWEKYCLNHCFSVPEGFSLPS+
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSD

Query:  DESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRT-E
        DESP VTS+S D  +DVDLDTELD LR  LSEVRKENIVLNQELQALERQTASSNSQ++ FNEALQLYEQ+SVN+MFQEM+ TASELR KIGKLKKR  E
Subjt:  DESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRT-E

Query:  ETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI
        E+KL  VEK+HTN DISHHHKGFSNAKLDDIQEFL+DLK +
Subjt:  ETKLAIVEKVHTNGDISHHHKGFSNAKLDDIQEFLSDLKKI

SwissProt top hits

e value

%identity

Alignment

Q2V0Z5 Protein MIS12 homolog

1.4e-48

45.75

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSD--RSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLP
        MEGSK E VF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK + SD  +SQ L+ GI++VR L+   +  RL +WE Y L  CF+VP+GF LP
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSD--RSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLP

Query:  SDDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLK-KR
          +ES   +S+  D  +D++LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+EM + ASELR  + +LK +R
Subjt:  SDDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLK-KR

Query:  TEETKLAIVEKVHTNGDISHHHKGFS----NAKLDDIQEFLSDLKKI
         + ++ A V++      + +H K FS    + KL+D+++F ++L+K+
Subjt:  TEETKLAIVEKVHTNGDISHHHKGFS----NAKLDDIQEFLSDLKKI

Arabidopsis top hits

e value

%identity

Alignment

AT5G35520.1 minichromosome instability 12 (mis12)-like

9.6e-50

45.75

Show/hide

Query:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSD--RSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLP
        MEGSK E VF+S+NLNPQ+FINEA+N+V+D VD AFDFY   AS +LK + SD  +SQ L+ GI++VR L+   +  RL +WE Y L  CF+VP+GF LP
Subjt:  MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSD--RSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLP

Query:  SDDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLK-KR
          +ES   +S+  D  +D++LD ELD LR+KL+ V K ++ L+ ELQALER + S    +   NEAL+LY++SS++++F+EM + ASELR  + +LK +R
Subjt:  SDDESPGVTSISHD--HDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLK-KR

Query:  TEETKLAIVEKVHTNGDISHHHKGFS----NAKLDDIQEFLSDLKKI
         + ++ A V++      + +H K FS    + KL+D+++F ++L+K+
Subjt:  TEETKLAIVEKVHTNGDISHHHKGFS----NAKLDDIQEFLSDLKKI

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGGAAGGAAGTAAAGGCGAAGTGGTGTTCAATTCACTCAATCTGAATCCACAGCTGTTCATTAATGAAGCTCTCAATACTGTCGACGATTTAGTTGATGATGCTTTCGA
TTTCTATCAATCACAAGCGTCGGCTGCCTTGAAGACCGAGAGCTCGGATAGATCTCAGGATCTTACCCTAGGAATATCGCAAGTCCGAGCGTTGGTCCAATTGGGTCTGC
ATAAGCGATTGGCCATGTGGGAAAAGTACTGCCTTAATCACTGTTTTTCAGTGCCTGAAGGTTTTTCTTTACCCTCAGATGATGAATCACCTGGTGTCACTTCAATAAGT
CATGACCATGACGTGGATCTGGATACAGAGCTTGATCTTTTGAGAAATAAGCTCTCCGAGGTCAGAAAAGAGAATATTGTATTAAACCAAGAACTGCAAGCTTTGGAAAG
GCAAACTGCTTCCAGTAACTCCCAAATCAGTCATTTCAACGAAGCATTACAACTATATGAGCAAAGTTCTGTGAATGACATGTTCCAGGAAATGATCAGAACTGCATCGG
AGCTTCGTGTGAAAATAGGAAAGCTGAAGAAAAGGACGGAGGAAACCAAGCTCGCTATTGTAGAGAAGGTTCATACAAATGGAGACATCTCTCACCATCACAAGGGTTTC
TCTAATGCTAAGCTGGATGATATTCAAGAATTTTTATCTGATTTGAAGAAGATTTGA

mRNA sequence

Show/hide mRNA sequence

Protein sequence

Show/hide protein sequence

MEGSKGEVVFNSLNLNPQLFINEALNTVDDLVDDAFDFYQSQASAALKTESSDRSQDLTLGISQVRALVQLGLHKRLAMWEKYCLNHCFSVPEGFSLPSDDESPGVTSIS
HDHDVDLDTELDLLRNKLSEVRKENIVLNQELQALERQTASSNSQISHFNEALQLYEQSSVNDMFQEMIRTASELRVKIGKLKKRTEETKLAIVEKVHTNGDISHHHKGF
SNAKLDDIQEFLSDLKKI

Cucsat.G14554 (gene) of Cucumber (B10) v3 genome