CuGenDBv2

Gene ID	Cucsat.G4066
Organism	Cucumis sativus L. var. sativus cv. B10 (Cucumber (B10) v3)
Description	Mediator of RNA polymerase II transcription subunit 32
Genome location	ctg105:3511894..3515057
RNA-Seq Expression	Cucsat.G4066
Synteny	Cucsat.G4066
Gene Ontology terms	GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0009631 - cold acclimation (biological process) GO:0010150 - leaf senescence (biological process) GO:0048364 - root development (biological process) GO:0016592 - mediator complex (cellular component)
InterPro domains	NA

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0045925.1 mediator of RNA polymerase II transcription subunit 32 [Cucumis melo var. makuwa]

6.06e-91

98.63

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQS TT LPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ

TYK13665.1 mediator of RNA polymerase II transcription subunit 32 [Cucumis melo var. makuwa]

2.47e-90

97.95

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQS TT LPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTED TQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ

XP_004148855.1 mediator of RNA polymerase II transcription subunit 32 [Cucumis sativus]

3.19e-92

100

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ

XP_008457936.1 PREDICTED: mediator of RNA polymerase II transcription subunit 32 [Cucumis melo]

2.16e-90

97.95

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQS TT LPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTED TQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ

XP_038902813.1 mediator of RNA polymerase II transcription subunit 32 [Benincasa hispida]

7.52e-91

97.95

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQ+ATT +PPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LIN7 Uncharacterized protein

1.54e-92

100

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ

A0A1S3C6T6 mediator of RNA polymerase II transcription subunit 32

1.04e-90

97.95

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQS TT LPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTED TQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ

A0A5A7TWZ7 Mediator of RNA polymerase II transcription subunit 32

2.94e-91

98.63

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQS TT LPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ

A0A5D3CQ46 Mediator of RNA polymerase II transcription subunit 32

1.20e-90

97.95

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQS TT LPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTED TQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ

A0A6J1JH28 mediator of RNA polymerase II transcription subunit 32-like isoform X2

4.33e-86

94.63

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSA--TTSLPPIS
        MDSLVDSLNNAYQEFV+GAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQ+A  TT LPPIS
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSA--TTSLPPIS

Query:  AVRLEQMSKAVRWLVIELQHGSGAAA-SASAHSHQSAPFDARFTEDATQ
        A+RLEQMSKAVRWLVIELQHGSGAAA SASAHSH SAPFDARFTEDATQ
Subjt:  AVRLEQMSKAVRWLVIELQHGSGAAA-SASAHSHQSAPFDARFTEDATQ

SwissProt top hits

e value

%identity

Alignment

Q84VW5 Mediator of RNA polymerase II transcription subunit 32

1.9e-45

67.32

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSG-------QSATTS
        MD++VDSLN AY++FV  +A VLE+ E++G QK   TD ALENFK+KWELFRVACDQAEEFVESVKQRIGSECLVDEATG     +G         A TS
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSG-------QSATTS

Query:  LPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
        LPPISAVRLEQMS+AVRWLV+ELQ GSG  A  S HS  S  FD+RF+ED+TQ
Subjt:  LPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ

Arabidopsis top hits

e value

%identity

Alignment

AT1G11760.1 MED32; Has 41 Blast hits to 40 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).

1.4e-46

67.32

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSG-------QSATTS
        MD++VDSLN AY++FV  +A VLE+ E++G QK   TD ALENFK+KWELFRVACDQAEEFVESVKQRIGSECLVDEATG     +G         A TS
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSG-------QSATTS

Query:  LPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ
        LPPISAVRLEQMS+AVRWLV+ELQ GSG  A  S HS  S  FD+RF+ED+TQ
Subjt:  LPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ

Sequences

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CDS sequence

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ATGGACAGCCTAGTGGACTCTTTAAATAACGCATATCAGGAGTTTGTTGCTGGAGCAGCTAATGTTCTTGAAGCAAATGAAGCCTCTGGGGCTCAGAAGACAGCAGCAAC
AGATGCCGCTCTTGAGAATTTTAAACAAAAATGGGAGCTATTCAGGGTTGCTTGTGATCAAGCAGAGGAGTTTGTAGAGTCTGTGAAGCAAAGGATAGGATCAGAGTGCC
TTGTTGACGAGGCCACTGGTTCACTGGCTGGCAAGTCTGGGCAGTCTGCCACCACTAGCCTTCCTCCAATCAGTGCTGTTCGATTGGAACAGATGAGCAAAGCGGTAAGA
TGGCTCGTGATTGAGTTGCAGCACGGCTCAGGAGCTGCTGCTTCTGCTTCAGCACACTCTCATCAGTCTGCTCCTTTTGATGCTAGATTTACTGAGGATGCAACCCAATA
G

mRNA sequence

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Protein sequence

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MDSLVDSLNNAYQEFVAGAANVLEANEASGAQKTAATDAALENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSATTSLPPISAVRLEQMSKAVR
WLVIELQHGSGAAASASAHSHQSAPFDARFTEDATQ

Cucsat.G4066 (gene) of Cucumber (B10) v3 genome