CuGenDBv2

Gene ID	Cucsat.G5500
Organism	Cucumis sativus L. var. sativus cv. B10 (Cucumber (B10) v3)
Description	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3
Genome location	ctg1269:420198..423534
RNA-Seq Expression	Cucsat.G5500
Synteny	Cucsat.G5500
Gene Ontology terms	GO:0001172 - transcription, RNA-templated (biological process) GO:0030422 - production of siRNA involved in RNA interference (biological process) GO:0032981 - mitochondrial respiratory chain complex I assembly (biological process) GO:0070919 - production of siRNA involved in chromatin silencing by small RNA (biological process) GO:0005634 - nucleus (cellular component) GO:0005743 - mitochondrial inner membrane (cellular component) GO:0031380 - nuclear RNA-directed RNA polymerase complex (cellular component) GO:0003723 - RNA binding (molecular function) GO:0003968 - RNA-directed 5'-3' RNA polymerase activity (molecular function)
InterPro domains	IPR007523 - NDUFAF3/Mth938 domain-containing protein IPR036748 - MTH938-like superfamily

Homology

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GenBank top hits

e value

%identity

Alignment

XP_008460070.1 PREDICTED: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3-like [Cucumis melo]

7.03e-71

92.37

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Query:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS
        MAARQKAVATLP+LIRTLKNDRRKPGFSQPLPSLR AFSLYDQINLIDNVP+DQLRFQRY+ETGFTVNGVDYEG+LLCVGNLLMSW+PKKFSEIT DSLS
Subjt:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS

Query:  IFQIVRPIPEILILGYGR
        IFQIVRPIPEIL+LG GR
Subjt:  IFQIVRPIPEILILGYGR

XP_031742645.1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3-like [Cucumis sativus]

9.08e-74

96.61

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Query:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS
        MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLR AFSLYDQINLIDNVPEDQLRFQRY+ETGFTVNGVDYEG+LLCVGNLLMSWTPKKFSEITSDSLS
Subjt:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS

Query:  IFQIVRPIPEILILGYGR
        IFQIVRPIPEILILG GR
Subjt:  IFQIVRPIPEILILGYGR

XP_031743290.1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3-like [Cucumis sativus]

9.08e-74

96.61

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Query:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS
        MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLR AFSLYDQINLIDNVPEDQLRFQRY+ETGFTVNGVDYEG+LLCVGNLLMSWTPKKFSEITSDSLS
Subjt:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS

Query:  IFQIVRPIPEILILGYGR
        IFQIVRPIPEILILG GR
Subjt:  IFQIVRPIPEILILGYGR

XP_031745505.1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3-like [Cucumis sativus]

5.23e-73

95.76

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Query:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS
        MAAR KAVATLPNLIRTLKNDRRKPGFSQPLPSLR AFSLYDQINLIDNVPEDQLRFQRY+ETGFTVNGVDYEG+LLCVGNLLMSWTPKKFSEITSDSLS
Subjt:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS

Query:  IFQIVRPIPEILILGYGR
        IFQIVRPIPEILILG GR
Subjt:  IFQIVRPIPEILILGYGR

XP_038906819.1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3-like [Benincasa hispida]

2.33e-69

90.68

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Query:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS
        MAARQKAVATLPNLIR+L+NDRR+PG SQPLPSLR AFSLYDQINLIDNVPEDQLRFQRY+ETGFTVNGVDYEG+LLCVGNLLMSW+PKKFSEIT +SLS
Subjt:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS

Query:  IFQIVRPIPEILILGYGR
        IFQIVRPIPEILILG GR
Subjt:  IFQIVRPIPEILILGYGR

TrEMBL top hits

e value

%identity

Alignment

A0A1S3CB84 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3

3.40e-71

92.37

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Query:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS
        MAARQKAVATLP+LIRTLKNDRRKPGFSQPLPSLR AFSLYDQINLIDNVP+DQLRFQRY+ETGFTVNGVDYEG+LLCVGNLLMSW+PKKFSEIT DSLS
Subjt:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS

Query:  IFQIVRPIPEILILGYGR
        IFQIVRPIPEIL+LG GR
Subjt:  IFQIVRPIPEILILGYGR

A0A5A7TES8 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3

3.40e-71

92.37

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Query:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS
        MAARQKAVATLP+LIRTLKNDRRKPGFSQPLPSLR AFSLYDQINLIDNVP+DQLRFQRY+ETGFTVNGVDYEG+LLCVGNLLMSW+PKKFSEIT DSLS
Subjt:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS

Query:  IFQIVRPIPEILILGYGR
        IFQIVRPIPEIL+LG GR
Subjt:  IFQIVRPIPEILILGYGR

A0A5D3DLM8 NADH dehydrogenase (Ubiquinone) 1 alpha subcomplex assembly factor 3-like

2.69e-66

93.58

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Query:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS
        MAARQKAVATLP+LIRTLKNDRRKPGFSQPLPSLR AFSLYDQINLIDNVP+DQLRFQRY+ETGFTVNGVDYEG+LLCVGNLLMSW+PKKFSEIT DSLS
Subjt:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS

Query:  IFQIVRPIP
        IFQIVRPIP
Subjt:  IFQIVRPIP

A0A6J1HIQ3 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3

2.15e-67

86.44

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Query:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS
        MAARQKAVATLPNLIR+++ DRR+PG SQPLPSLR AFSLYDQINLIDNVPEDQLRFQRY+ETGFTVNG+DYEG+LLCVGNLLMSW+PKKFSEIT +SLS
Subjt:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS

Query:  IFQIVRPIPEILILGYGR
        IFQI+RPIPEIL+LG GR
Subjt:  IFQIVRPIPEILILGYGR

A0A6J1KD25 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3

1.01e-65

84.75

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Query:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS
        MAARQKAVATLPNLIR+++ DRR+PG SQPLPSLR AFSLYDQINLIDNVPEDQLRFQ Y+ETGFTVNG+DYEG+LLCVG+LLMSW+PKKFSEIT +SLS
Subjt:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS

Query:  IFQIVRPIPEILILGYGR
        IFQI+RPIPEIL+LG GR
Subjt:  IFQIVRPIPEILILGYGR

SwissProt top hits

e value

%identity

Alignment

O08776 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3

4.9e-04

29.33

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Query:  QINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLSIFQIVRPIPEILILGYG
        +I+L+ N     +    Y+  GFT+NG    G    +   ++ W      +IT +S SIF ++ P  EI+++G G
Subjt:  QINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLSIFQIVRPIPEILILGYG

Arabidopsis top hits

e value

%identity

Alignment

AT2G44525.1 Protein of unknown function (DUF498/DUF598)

3.2e-43

72.27

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Query:  MAARQKAVATLPNLIRTLKNDRRKPGFSQP-LPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSL
        MA R K++ATLP LI++++  +  P  S P LPSLR AFSLYDQINLIDNVPEDQLRFQ +++T FTVNGV YEG+LLCVGNLLMSW+P+KFSEIT+DSL
Subjt:  MAARQKAVATLPNLIRTLKNDRRKPGFSQP-LPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSL

Query:  SIFQIVRPIPEILILGYGR
        SIFQ VRPIPE+LI+G GR
Subjt:  SIFQIVRPIPEILILGYGR

AT3G60150.1 Protein of unknown function (DUF498/DUF598)

5.0e-44

73.73

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Query:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS
        MA RQKA+ATLP LI++++N+  K   S  LPSLR AFSLYDQINLIDNVPEDQLRFQ + ET FTVNGV YEG+LLCVGNLLMSW+P+ FS+IT+DSLS
Subjt:  MAARQKAVATLPNLIRTLKNDRRKPGFSQPLPSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLS

Query:  IFQIVRPIPEILILGYGR
        IFQ VRPIPE+LI+G GR
Subjt:  IFQIVRPIPEILILGYGR

Sequences

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CDS sequence

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GCCGTCCACCGATCCCTTCTCCTCCGTTGTCTGCACTGCCGCGCGACTCTCCGGCCGTCTGTATACGGTCTCTCCGTCGCATGTCGTCTCTTCGCAAAGCTACTGCTCGA
TTTCTTTCGCAAAACACTAAATGAAAAATGCAATTATATCAAAGTTGATGTCCTAAAGAAGAAAAGTAAAAGAGAAAAAGGGATTTGGGGGCGAAACTCCGGGACGGGGA
CAGAGGGCCTGAGAAAAATGGCGGCAAGGCAGAAAGCGGTGGCGACGCTGCCTAATCTAATTAGAACCCTTAAAAATGATCGCCGGAAGCCTGGTTTCAGCCAGCCTCTT
CCGTCGTTGAGATGTGCCTTTTCACTTTACGATCAGATCAATCTCATCGACAATGTCCCTGAAGATCAGCTTCGTTTTCAACGGTATAGTGAGACGGGGTTCACTGTGAA
TGGAGTGGATTATGAGGGCAACTTGCTTTGTGTTGGGAATTTGTTGATGTCTTGGACTCCTAAGAAATTTTCGGAAATTACGTCCGATAGCTTATCTATCTTCCAGATTG
TGCGCCCTATTCCAGAAATTCTAATACTTGGCTATGGGAGATAA

mRNA sequence

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Protein sequence

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AVHRSLLLRCLHCRATLRPSVYGLSVACRLFAKLLLDFFRKTLNEKCNYIKVDVLKKKSKREKGIWGRNSGTGTEGLRKMAARQKAVATLPNLIRTLKNDRRKPGFSQPL
PSLRCAFSLYDQINLIDNVPEDQLRFQRYSETGFTVNGVDYEGNLLCVGNLLMSWTPKKFSEITSDSLSIFQIVRPIPEILILGYGR

Cucsat.G5500 (gene) of Cucumber (B10) v3 genome