CuGenDBv2

Gene ID	Cucsat.G8011
Organism	Cucumis sativus L. var. sativus cv. B10 (Cucumber (B10) v3)
Description	Transcription factor IIIB 90 kDa subunit-like isoform X3
Genome location	ctg1556:2322043..2324146
RNA-Seq Expression	Cucsat.G8011
Synteny	Cucsat.G8011
Gene Ontology terms	GO:0006352 - DNA-templated transcription, initiation (biological process) GO:0006383 - transcription by RNA polymerase III (biological process) GO:0070897 - transcription preinitiation complex assembly (biological process) GO:0000126 - transcription factor TFIIIB complex (cellular component) GO:0016020 - membrane (cellular component) GO:0016021 - integral component of membrane (cellular component) GO:0000995 - RNA polymerase III general transcription initiation factor activity (molecular function)
InterPro domains	IPR000812 - Transcription factor TFIIB IPR011665 - Brf1, TBP-binding domain IPR029529 - Transcription factor IIIB subunit Brf1

Homology

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GenBank top hits

e value

%identity

Alignment

KAE8649624.1 hypothetical protein Csa_012863 [Cucumis sativus]

4.04e-160

100

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Query:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ
        MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ
Subjt:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ

Query:  ADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDI
        ADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDI
Subjt:  ADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDI

Query:  EGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
        EGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
Subjt:  EGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY

XP_004142699.1 uncharacterized protein LOC101217202 isoform X2 [Cucumis sativus]

2.76e-162

100

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Query:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ
        MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ
Subjt:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ

Query:  ADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDI
        ADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDI
Subjt:  ADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDI

Query:  EGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
        EGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
Subjt:  EGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY

XP_008444162.1 PREDICTED: uncharacterized protein LOC103487591 isoform X2 [Cucumis melo]

4.97e-119

81.01

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Query:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ
        MK ADASLF MF +HKDKSSPS+DDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLN+VGA+AVVKKSKKR+RK EAPIN PAQ
Subjt:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ

Query:  ADTG-TTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFD
        ADT  TT EM IKKRLSSKF+FDVLDKL  DTPAPDSSEKQGASSCKENSSSGQ HC NELEDDFEDN                     HTET+FEEN D
Subjt:  ADTG-TTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFD

Query:  IEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
        IEG EDPTETYGNEYYDFQ+EEEYGYGY+DYGGEEEY
Subjt:  IEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY

XP_011653794.1 uncharacterized protein LOC101217202 isoform X1 [Cucumis sativus]

1.93e-160

99.58

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Query:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKK-RQRKTEAPINMPA
        MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKK RQRKTEAPINMPA
Subjt:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKK-RQRKTEAPINMPA

Query:  QADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFD
        QADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFD
Subjt:  QADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFD

Query:  IEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
        IEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
Subjt:  IEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY

XP_016899849.1 PREDICTED: uncharacterized protein LOC103487591 isoform X1 [Cucumis melo]

3.46e-117

80.67

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Query:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKK-RQRKTEAPINMPA
        MK ADASLF MF +HKDKSSPS+DDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLN+VGA+AVVKKSKK R+RK EAPIN PA
Subjt:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKK-RQRKTEAPINMPA

Query:  QADTG-TTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENF
        QADT  TT EM IKKRLSSKF+FDVLDKL  DTPAPDSSEKQGASSCKENSSSGQ HC NELEDDFEDN                     HTET+FEEN 
Subjt:  QADTG-TTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENF

Query:  DIEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
        DIEG EDPTETYGNEYYDFQ+EEEYGYGY+DYGGEEEY
Subjt:  DIEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY

TrEMBL top hits

e value

%identity

Alignment

A0A0A0L0J7 BRF1 domain-containing protein

1.17e-161

100

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Query:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ
        MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ
Subjt:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ

Query:  ADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDI
        ADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDI
Subjt:  ADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDI

Query:  EGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
        EGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
Subjt:  EGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY

A0A1S3B993 uncharacterized protein LOC103487591 isoform X2

2.41e-119

81.01

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Query:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ
        MK ADASLF MF +HKDKSSPS+DDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLN+VGA+AVVKKSKKR+RK EAPIN PAQ
Subjt:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ

Query:  ADTG-TTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFD
        ADT  TT EM IKKRLSSKF+FDVLDKL  DTPAPDSSEKQGASSCKENSSSGQ HC NELEDDFEDN                     HTET+FEEN D
Subjt:  ADTG-TTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFD

Query:  IEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
        IEG EDPTETYGNEYYDFQ+EEEYGYGY+DYGGEEEY
Subjt:  IEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY

A0A1S3B9Q2 uncharacterized protein LOC103487591 isoform X3

1.69e-101

72.46

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Query:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ
        MK ADASLF MF +HKDKSSPS+DDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLN+VGA+AVVKKS                
Subjt:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ

Query:  ADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDI
                   KKRLSSKF+FDVLDKL  DTPAPDSSEKQGASSCKENSSSGQ HC NELEDDFEDN                     HTET+FEEN DI
Subjt:  ADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDI

Query:  EGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
        EG EDPTETYGNEYYDFQ+EEEYGYGY+DYGGEEEY
Subjt:  EGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY

A0A1S4DV37 uncharacterized protein LOC103487591 isoform X1

1.67e-117

80.67

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Query:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKK-RQRKTEAPINMPA
        MK ADASLF MF +HKDKSSPS+DDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLN+VGA+AVVKKSKK R+RK EAPIN PA
Subjt:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKK-RQRKTEAPINMPA

Query:  QADTG-TTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENF
        QADT  TT EM IKKRLSSKF+FDVLDKL  DTPAPDSSEKQGASSCKENSSSGQ HC NELEDDFEDN                     HTET+FEEN 
Subjt:  QADTG-TTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENF

Query:  DIEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
        DIEG EDPTETYGNEYYDFQ+EEEYGYGY+DYGGEEEY
Subjt:  DIEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY

A0A5D3E7S6 Transcription factor IIIB 90 kDa subunit-like isoform X3

2.41e-119

81.01

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Query:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ
        MK ADASLF MF +HKDKSSPS+DDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLN+VGA+AVVKKSKKR+RK EAPIN PAQ
Subjt:  MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQ

Query:  ADTG-TTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFD
        ADT  TT EM IKKRLSSKF+FDVLDKL  DTPAPDSSEKQGASSCKENSSSGQ HC NELEDDFEDN                     HTET+FEEN D
Subjt:  ADTG-TTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFD

Query:  IEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY
        IEG EDPTETYGNEYYDFQ+EEEYGYGY+DYGGEEEY
Subjt:  IEGEEDPTETYGNEYYDFQDEEEYGYGYNDYGGEEEY

SwissProt top hits

e value

%identity

Alignment

Q9P6R0 Transcription factor IIIB 60 kDa subunit

1.8e-05

30.28

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Query:  DDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQADTG--TTREMQIKKRLSSKFNF
        ++   L DV D E+   L ++ E   K  +W ++NKDYL ++ AK   L       +V++ +KR+R           ADT   + +EM  ++  S K N+
Subjt:  DDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQADTG--TTREMQIKKRLSSKFNF

Query:  DVLDKLFSD
        + LD LF +
Subjt:  DVLDKLFSD

Arabidopsis top hits

e value

%identity

Alignment

AT3G09360.1 Cyclin/Brf1-like TBP-binding protein

9.0e-16

32.22

Show/hide

Query:  DKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVG----------------------ASAVVKKSKKRQRKTEAP
        ++ + + D+S++  D+ D EVN Y+NN +E HYK I W ++NKDYL++QAAK+  L                          A A   + +K+Q+K E  
Subjt:  DKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVG----------------------ASAVVKKSKKRQRKTEAP

Query:  INM--PAQADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTET
         N   PA A     R +  KKRLSS  N+DVL+ LF DT AP+ S K+  +               ++E   E+N    S + H   E ED  E++    
Subjt:  INM--PAQADTGTTREMQIKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTET

Query:  MFEENFDIEGEEDPTETYGNEYYDFQDEEEYGYGYNDYG
          EE  ++E  +  T+    E +  +DEEE   G ND+G
Subjt:  MFEENFDIEGEEDPTETYGNEYYDFQDEEEYGYGYNDYG

Sequences

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CDS sequence

Show/hide CDS sequence

ATGAAAACTGCTGATGCATCTTTATTCGGCATGTTCTTCAAACACAAAGATAAAAGTTCTCCATCCGATGATGATTCAGAGGACCTTGGTGATGTTTTTGATAGTGAGGT
GAACTCTTACCTAAACAACAGGAAGGAGGCACATTATAAAAAGATTATTTGGGAGCAAATTAACAAAGACTATCTTCAGGACCAAGCAGCTAAGAAACAAGGCCTTAATG
TCGTTGGAGCTTCAGCTGTGGTTAAAAAATCAAAGAAGAGGCAAAGGAAAACAGAAGCTCCAATAAACATGCCTGCTCAAGCCGATACAGGAACTACACGTGAAATGCAA
ATTAAAAAGAGGCTCAGTTCAAAATTCAACTTTGACGTGTTGGACAAACTCTTCTCTGATACACCTGCACCTGATTCAAGCGAAAAGCAGGGAGCCTCAAGTTGTAAAGA
GAATAGTTCATCTGGTCAAATCCATTGTAGGAACGAGCTGGAAGACGACTTCGAAGACAATCTGAATAGTTCATCTGGTCAAATCCATTGTAGGAACGAGCTGGAAGACA
ACTTCGAAGACAATCATACTGAGACGATGTTTGAAGAAAACTTCGACATTGAAGGTGAAGAAGATCCCACAGAAACTTATGGTAATGAGTATTATGATTTCCAAGATGAG
GAAGAGTATGGCTACGGATACAATGACTATGGTGGTGAGGAGGAATATTAG

mRNA sequence

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Protein sequence

Show/hide protein sequence

MKTADASLFGMFFKHKDKSSPSDDDSEDLGDVFDSEVNSYLNNRKEAHYKKIIWEQINKDYLQDQAAKKQGLNVVGASAVVKKSKKRQRKTEAPINMPAQADTGTTREMQ
IKKRLSSKFNFDVLDKLFSDTPAPDSSEKQGASSCKENSSSGQIHCRNELEDDFEDNLNSSSGQIHCRNELEDNFEDNHTETMFEENFDIEGEEDPTETYGNEYYDFQDE
EEYGYGYNDYGGEEEY

Cucsat.G8011 (gene) of Cucumber (B10) v3 genome