CuGenDBv2

Gene ID	HG10005146
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial
Genome location	Chr08:23186981..23191044
RNA-Seq Expression	HG10005146
Synteny	HG10005146
Gene Ontology terms	GO:0030488 - tRNA methylation (biological process) GO:0070475 - rRNA base methylation (biological process) GO:0005737 - cytoplasm (cellular component) GO:0008173 - RNA methyltransferase activity (molecular function) GO:0046872 - metal ion binding (molecular function) GO:0051539 - 4 iron, 4 sulfur cluster binding (molecular function)
InterPro domains	IPR043459 - NFD6/NOXY2-like

Homology

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GenBank top hits

e value

%identity

Alignment

KAG7029191.1 hypothetical protein SDJN02_07528, partial [Cucurbita argyrosperma subsp. argyrosperma]

4.9e-36

84.11

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Query:  MAACCSWKTLRISSSSARALLHRSSPS-PSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGC
        MAAC +WKTLRISSSSAR LL RSS S PSASH VSKPT+SA LP AKPSASSR SV KLTNFRLPVELSSVQSLMPLH+ TASALFTSLLSLHNNSWGC
Subjt:  MAACCSWKTLRISSSSARALLHRSSPS-PSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGC

Query:  LSEAKMS
        LSE  +S
Subjt:  LSEAKMS

XP_004145570.1 uncharacterized protein LOC101210124 [Cucumis sativus]

1.4e-35

81.55

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Query:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL
        MAACCSWKT+ ISSSSAR LLHRSS SPSASHFVS PT+S GLP AKP ASSR SV +LTNFRLPVEL+S+QSLMPL +VTAS LFTSLLS HNNSWGCL
Subjt:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL

Query:  SEA
        S+A
Subjt:  SEA

XP_022961783.1 protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial [Cucurbita moschata]

3.2e-35

86.41

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Query:  MAACCSWKTLRISSSSARALLHRSSPS-PSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGC
        MAAC +WKTLRISSSSAR LL RSS S PSASH VSKPT+SA LP AKPSASSR SV KLTNFRLPVELSSVQSLMPLH+ TASALFTSLLSLHNNSWGC
Subjt:  MAACCSWKTLRISSSSARALLHRSSPS-PSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGC

Query:  LSE
        LSE
Subjt:  LSE

XP_022996536.1 uncharacterized protein LOC111491756 isoform X2 [Cucurbita maxima]

4.4e-37

87.38

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Query:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL
        MAAC +WKTLRISSSSAR LL RSS SPSASH VSKPT+SA LP AKPSASSR SV KLTNFRLPVELSSVQSLMPLH+ TASALFTSLLSLHNNSWGCL
Subjt:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL

Query:  SEA
        SEA
Subjt:  SEA

XP_038885251.1 protein NUCLEAR FUSION DEFECTIVE 6, mitochondrial isoform X2 [Benincasa hispida]

2.8e-39

91.18

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Query:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL
        MAACCSWKTLRISSSSAR LLHRSS SPSASHFVSKPT+SAGLP AKP ASSR  VQKLTNFRLPVELSSVQSLMPLH+VTASALFTSLLSLHNNSWG L
Subjt:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL

Query:  SE
        SE
Subjt:  SE

TrEMBL top hits

e value

%identity

Alignment

A0A0A0L605 Radical_SAM domain-containing protein

6.9e-36

81.55

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Query:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL
        MAACCSWKT+ ISSSSAR LLHRSS SPSASHFVS PT+S GLP AKP ASSR SV +LTNFRLPVEL+S+QSLMPL +VTAS LFTSLLS HNNSWGCL
Subjt:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL

Query:  SEA
        S+A
Subjt:  SEA

A0A5D3D8K9 Dual-specificity RNA methyltransferase RlmN isoform X1

3.5e-32

78.64

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Query:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL
        MAACCSWKT+ ISSSSARALLHR S SPSASHFVS PT+S GL  A+P+ASSR SVQ+LTNF LPVEL+S+QSLMPL +VTASALFTSLLS HNNS  CL
Subjt:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL

Query:  SEA
        S+A
Subjt:  SEA

A0A6J1E309 protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial

4.4e-35

82.35

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Query:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL
        MAACC+WKTLR+SSSS R L+ RSS S SASHFVS+PT   GLP AKP+AS R SVQKLTN RLPVELSSVQSLMPLH+VTASALFTSLLSLHNNSWGCL
Subjt:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL

Query:  SE
        SE
Subjt:  SE

A0A6J1HCT7 protein NUCLEAR FUSION DEFECTIVE 6, chloroplastic/mitochondrial

1.5e-35

86.41

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Query:  MAACCSWKTLRISSSSARALLHRSSPS-PSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGC
        MAAC +WKTLRISSSSAR LL RSS S PSASH VSKPT+SA LP AKPSASSR SV KLTNFRLPVELSSVQSLMPLH+ TASALFTSLLSLHNNSWGC
Subjt:  MAACCSWKTLRISSSSARALLHRSSPS-PSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGC

Query:  LSE
        LSE
Subjt:  LSE

A0A6J1K524 uncharacterized protein LOC111491756 isoform X2

2.1e-37

87.38

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Query:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL
        MAAC +WKTLRISSSSAR LL RSS SPSASH VSKPT+SA LP AKPSASSR SV KLTNFRLPVELSSVQSLMPLH+ TASALFTSLLSLHNNSWGCL
Subjt:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCL

Query:  SEA
        SEA
Subjt:  SEA

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G55205.1 unknown protein

6.8e-04

34.44

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Query:  SSARALLHRSSPSPSASHFVSKPTIS-AGLPFAKPSASSRLSVQKLT-NFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCLSE
        +S+  +   SS   S +  +SK  +  + LP    S+S   +  ++  +FRLPVELS   S++PLH+  ASA   S LS+ + SWG + +
Subjt:  SSARALLHRSSPSPSASHFVSKPTIS-AGLPFAKPSASSRLSVQKLT-NFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCLSE

AT1G55205.2 unknown protein

5.2e-04

33.7

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Query:  SSARALLHRSSPSPSASHFVSKPTIS-AGLPFAKPSASSRLSVQKLT-NFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCLSEAK
        +S+  +   SS   S +  +SK  +  + LP    S+S   +  ++  +FRLPVELS   S++PLH+  ASA   S LS+ + SWG + + +
Subjt:  SSARALLHRSSPSPSASHFVSKPTIS-AGLPFAKPSASSRLSVQKLT-NFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCLSEAK

AT1G70350.1 unknown protein

2.5e-14

54.37

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Query:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASS-RLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGC
        MAA    + L+ SSSS + L  RSS + SA    SK   SAG+ F   S+SS R S+++LT  R+PVELS+  SL+PLH+VTASAL TSLLSL N +W C
Subjt:  MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASS-RLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGC

Query:  LSE
        LSE
Subjt:  LSE

Sequences

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CDS sequence

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ATGGCGGCTTGCTGTTCCTGGAAAACCCTACGAATTTCTTCCTCTTCCGCTAGAGCTCTCCTTCACCGTTCCTCACCCTCTCCCTCTGCATCTCATTTTGTGTCCAAACC
TACTATATCTGCCGGACTTCCTTTCGCAAAGCCGTCCGCATCTTCCCGCTTATCTGTTCAGAAGCTTACAAACTTCAGGCTTCCAGTGGAGTTATCAAGCGTGCAGTCTC
TAATGCCATTGCACAATGTTACTGCTTCTGCCTTGTTTACTTCGTTGCTTTCTTTGCATAACAACAGTTGGGGTTGTCTATCTGAAGCGAAAATGTCCCGACATTCATTC
AAGAATGAGGGAGTTGGGAGGAGAGGCAGGCATGGTGCTGGTCGACCAGGAAAAGTAACTACTTCGAACAACAATTCTGCCCATCCTTTTCCATCAACATCAATCAAAGA
TGAGAATGAAGAAAATCAAGAAGGAGATTGA

mRNA sequence

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Protein sequence

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MAACCSWKTLRISSSSARALLHRSSPSPSASHFVSKPTISAGLPFAKPSASSRLSVQKLTNFRLPVELSSVQSLMPLHNVTASALFTSLLSLHNNSWGCLSEAKMSRHSF
KNEGVGRRGRHGAGRPGKVTTSNNNSAHPFPSTSIKDENEENQEGD

HG10005146 (gene) of Bottle gourd (Hangzhou Gourd) v1 genome