CuGenDBv2

Gene ID	HG10008489
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	protein MIS12 homolog
Genome location	Chr10:23575340..23578307
RNA-Seq Expression	HG10008489
Synteny	HG10008489
Gene Ontology terms	GO:0000070 - mitotic sister chromatid segregation (biological process) GO:0034501 - protein localization to kinetochore (biological process) GO:0051301 - cell division (biological process) GO:0051382 - kinetochore assembly (biological process) GO:0000818 - nuclear MIS12/MIND complex (cellular component)
InterPro domains	IPR008685 - Centromere protein Mis12

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAA0058004.1 Centromere protein Mis12 [Cucumis melo var. makuwa]

1.5e-113

89.12

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGIS+VR LVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLEET
        DESPGVTSI+HD DVDLDTELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQEMI  ASELR KIGK+KKRR EE 
Subjt:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLEET

Query:  KLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL
        KL K+EKVHTNGDISHHHNGF NAKLDDIQEFL+DLK +
Subjt:  KLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL

KAA0058032.1 Centromere protein Mis12 [Cucumis melo var. makuwa]

3.4e-113

88.7

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASA LKTEGSDRSQDLTLGIS+VR LVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLEET
        DESPGVTSI+HD DVDLDTELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQEMI  ASELR+KIGK+KKRR EE 
Subjt:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLEET

Query:  KLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL
        KL K+EKVHTNGDISHHHNGF NAKLDDIQEFL+DLK +
Subjt:  KLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL

KAG6589575.1 Protein MIS12-like protein, partial [Cucurbita argyrosperma subsp. sororia]

1.7e-112

88.38

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Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL LGISRVRT VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLE
        DESP VTS+S D  YDVDLDTELDFLRNKLSE RKEN+VL+QELQALERQTASSNSQ+N  NEALQLYEQNSVNEMFQEM+  ASELR KIGKLKKRR+E
Subjt:  DESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLE

Query:  ETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL
        E+KLTK+EK+HTNGDISHHH GF NAKLDDIQEFLADLK L
Subjt:  ETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL

XP_023515499.1 protein MIS12 homolog isoform X2 [Cucurbita pepo subsp. pepo]

2.6e-113

88.8

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL+LGISRVRT VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLE
        DESP VTS+S D  YDVDLDTELDFLRNKLSE RKEN+VL+QELQALERQTASSNSQ+N  NEALQLYEQNSVNEMFQEM+  ASELR KIGKLKKRR+E
Subjt:  DESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLE

Query:  ETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL
        E+KLTK+EK+HTNGDISHHH GF NAKLDDIQEFLADLKTL
Subjt:  ETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL

XP_038879945.1 protein MIS12 homolog [Benincasa hispida]

6.2e-115

90.79

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAF FYQSQASA LKTEGSDRSQDLTLGISRVR LVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLEET
        D+SPGVTSISHDYDV LDTELDFLRNKLSE RKENIVL+QELQALERQTA+SNSQINH NEALQLYEQNSVNEMFQEM+  ASELR KIGKLKKRR+EE+
Subjt:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLEET

Query:  KLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL
        KLTK+EKVHTNGDISH+HNG  NAKLDDI+ FL DLKTL
Subjt:  KLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL

TrEMBL top hits

e value

%identity

Alignment

A0A5A7UQ95 Centromere protein Mis12

1.6e-113

88.7

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASA LKTEGSDRSQDLTLGIS+VR LVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLEET
        DESPGVTSI+HD DVDLDTELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQEMI  ASELR+KIGK+KKRR EE 
Subjt:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLEET

Query:  KLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL
        KL K+EKVHTNGDISHHHNGF NAKLDDIQEFL+DLK +
Subjt:  KLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL

A0A5A7US57 Centromere protein Mis12

7.4e-114

89.12

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE+VF+SLNLNPQLFINEALN VDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGIS+VR LVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLEET
        DESPGVTSI+HD DVDLDTELD LRNKLSE RKENIVL+QELQALERQTASSNSQI+H NEALQLYEQ+SVN+MFQEMI  ASELR KIGK+KKRR EE 
Subjt:  DESPGVTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLEET

Query:  KLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL
        KL K+EKVHTNGDISHHHNGF NAKLDDIQEFL+DLK +
Subjt:  KLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL

A0A6J1E0A1 protein MIS12 homolog

2.6e-111

87.55

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL LGISRVRT VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLE
        DESP VTS+S D  YDVDLDTELDFLRNKLSE RKENIVL+QELQALERQ ASSNSQ+N  NEALQLYE+NSVNEMFQEM+  ASELR KIGKLKKRR+E
Subjt:  DESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLE

Query:  ETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL
        E+KLTK+EK+HTNGD SHHH GF NAKLDDIQEFLADLK L
Subjt:  ETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL

A0A6J1JD43 protein MIS12 homolog isoform X1

6.4e-110

87.6

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL LGISRVRT VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ-EMINIASELRTKIGKLKKRRL
        DESP VTS+S D  YDVDLDTELDFLR  LSE RKENIVL+QELQALERQTASSNSQ+N  NEALQLYEQNSVNEMFQ EM+  ASELR KIGKLKKRR+
Subjt:  DESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQ-EMINIASELRTKIGKLKKRRL

Query:  EETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL
        EE+KLTK+EK+HTN DISHHH GF NAKLDDIQEFLADLKTL
Subjt:  EETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL

A0A6J1JME3 protein MIS12 homolog isoform X2

2.6e-111

87.97

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN
        MEGSKGE VFDSLNLNPQLFINEALNIVDDLVDDAFDFYQ+QASA+LKTEGSDRSQDL LGISRVRT VQ GLDKRLAMWEKYCLNHCFSVPEGFSLPSN
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSN

Query:  DESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLE
        DESP VTS+S D  YDVDLDTELDFLR  LSE RKENIVL+QELQALERQTASSNSQ+N  NEALQLYEQNSVNEMFQEM+  ASELR KIGKLKKRR+E
Subjt:  DESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLE

Query:  ETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL
        E+KLTK+EK+HTN DISHHH GF NAKLDDIQEFLADLKTL
Subjt:  ETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL

SwissProt top hits

e value

%identity

Alignment

Q2V0Z5 Protein MIS12 homolog

2.8e-54

47.74

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSD--RSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLP
        MEGSK EAVFDS+NLNPQ+FINEA+N V+D VD AFDFY   AS +LK +GSD  +SQ L+ GI+RVR L+   +D RL +WE Y L  CF+VP+GF LP
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSD--RSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLP

Query:  SNDESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRR
         ++ES   +S+  D  YD++LD ELD LR+KL+   K ++ L  ELQALER + S    +  +NEAL+LY+++S++E+F+EM  +ASELR  + +LK RR
Subjt:  SNDESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRR

Query:  LEETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL
        ++ ++  K++++  +G         F+ KL+D+++F A+L+ +
Subjt:  LEETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL

Arabidopsis top hits

e value

%identity

Alignment

AT5G35520.1 minichromosome instability 12 (mis12)-like

2.0e-55

47.74

Show/hide

Query:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSD--RSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLP
        MEGSK EAVFDS+NLNPQ+FINEA+N V+D VD AFDFY   AS +LK +GSD  +SQ L+ GI+RVR L+   +D RL +WE Y L  CF+VP+GF LP
Subjt:  MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSD--RSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLP

Query:  SNDESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRR
         ++ES   +S+  D  YD++LD ELD LR+KL+   K ++ L  ELQALER + S    +  +NEAL+LY+++S++E+F+EM  +ASELR  + +LK RR
Subjt:  SNDESPGVTSISHD--YDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRR

Query:  LEETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL
        ++ ++  K++++  +G         F+ KL+D+++F A+L+ +
Subjt:  LEETKLTKIEKVHTNGDISHHHNGFFNAKLDDIQEFLADLKTL

Sequences

Show/hide sequences

CDS sequence

Show/hide CDS sequence

ATGGAAGGAAGTAAAGGCGAAGCAGTATTCGATTCACTGAATCTGAATCCACAGCTCTTCATTAATGAAGCTCTCAATATTGTCGACGATTTGGTTGATGACGCT
TTTGATTTCTATCAATCACAAGCATCGGCCGCCTTGAAGACCGAGGGCTCCGATAGATCCCAGGATCTTACCCTGGGAATATCGCGGGTCCGAACCTTGGTCCAA
TTGGGTCTGGATAAGCGGTTGGCCATGTGGGAGAAGTACTGTCTCAATCATTGTTTTTCAGTTCCTGAAGGTTTTTCTTTACCCTCAAATGATGAATCACCTGGT
GTCACTTCAATAAGTCATGACTATGACGTGGATCTGGATACAGAGTTAGATTTTTTGAGGAATAAGCTCTCTGAGGCCAGAAAAGAGAATATTGTATTGAGCCAA
GAACTACAAGCTTTGGAAAGGCAAACTGCTTCCAGTAACTCCCAAATCAATCATTTGAACGAAGCATTACAATTATATGAGCAAAATTCTGTGAATGAGATGTTC
CAGGAAATGATAAACATTGCATCGGAGCTGCGAACGAAAATAGGAAAGCTGAAGAAAAGGAGGTTGGAGGAAACCAAGCTCACTAAAATAGAGAAGGTTCATACA
AACGGAGACATTTCTCACCATCACAATGGTTTCTTTAATGCTAAGCTGGATGATATTCAAGAATTTTTAGCTGATTTGAAGACCCTTTGA

mRNA sequence

Show/hide mRNA sequence

Protein sequence

Show/hide protein sequence

MEGSKGEAVFDSLNLNPQLFINEALNIVDDLVDDAFDFYQSQASAALKTEGSDRSQDLTLGISRVRTLVQLGLDKRLAMWEKYCLNHCFSVPEGFSLPSNDESPG
VTSISHDYDVDLDTELDFLRNKLSEARKENIVLSQELQALERQTASSNSQINHLNEALQLYEQNSVNEMFQEMINIASELRTKIGKLKKRRLEETKLTKIEKVHT
NGDISHHHNGFFNAKLDDIQEFLADLKTL

HG10008489 (gene) of Bottle gourd (Hangzhou Gourd) v1 genome