CuGenDBv2

Gene ID	HG10009367
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	tRNA (Guanine(9)-N1)-methyltransferase
Genome location	Chr06:5154242..5159115
RNA-Seq Expression	HG10009367
Synteny	HG10009367
Gene Ontology terms	GO:0002939 - tRNA N1-guanine methylation (biological process) GO:0005634 - nucleus (cellular component) GO:0000049 - tRNA binding (molecular function) GO:0052905 - tRNA (guanine(9)-N(1))-methyltransferase activity (molecular function)
InterPro domains	IPR007356 - tRNA (guanine-N1-)-methyltransferase, eukaryotic IPR016009 - tRNA methyltransferase TRMD/TRM10-type domain IPR016653 - tRNA (guanine(9)-N(1))-methyltransferase TRM10/TRM10A IPR028564 - tRNA methyltransferase TRM10-type domain IPR038459 - tRNA methyltransferase TRM10-type domain superfamily

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAG6583717.1 hypothetical protein SDJN03_19649, partial [Cucurbita argyrosperma subsp. sororia]

6.1e-161

91.32

Show/hide

Query:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        MEA EEDQSAKMDPP SP  CNP  PPLSKNARKKLLKQQRY+AKKAEKK Q KEQKKKDIERKRKEWEETLASVTEEER+KLIESR+SLRKERMGKRSE
Subjt:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
        E+EKKIERLN AKDCGQ IVIDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIE+EHRSYIDALQDQKENLV
Subjt:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV

Query:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWKASFF+VVPQRKRCEA+ NS
Subjt:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHT
        DH VDGEENGD   DDE ETKKTKCDEVS +NHT
Subjt:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHT

XP_022927615.1 tRNA (guanine(9)-N1)-methyltransferase [Cucurbita moschata]

2.6e-159

90.42

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Query:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        MEA EEDQSAKMDPP SP  CNP  PPLSKNARKKLLKQQRY+AKKAEKK Q KEQKKKDIERKRKEWEETLASVTEEER+KLIESR+SLRKERMGKRSE
Subjt:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
        E+EKKIERLN AKDCGQ IVIDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQD KENLV
Subjt:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV

Query:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITM KAQEQGIQTAKLPIGSYLKMSSSQVLTVNQV+EILL+Y+ETRDWKASFF+VVPQRKRCEA+ NS
Subjt:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHT
        DH VDG+ENGD   DDE ETKKTKCDEVS +NHT
Subjt:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHT

XP_022973151.1 tRNA (guanine(9)-N1)-methyltransferase [Cucurbita maxima]

5.1e-160

90.72

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Query:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        MEAIEEDQSAKMDPP SP  CNP  PPLSKNARKKLLKQQRY+AKKAEKK Q KEQKKKDIERKRKEWE+TLASVTEEER+KLIESRRSLRKERMGKRSE
Subjt:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
        E+EKKIERLN AKDCGQ I+IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
Subjt:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV

Query:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWKASFF+VVPQRKRCEA+ NS
Subjt:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHT
        DH VDGEENGD   DDE E+KK KC EVS +NHT
Subjt:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHT

XP_023520714.1 tRNA (guanine(9)-N1)-methyltransferase [Cucurbita pepo subsp. pepo]

2.7e-161

91.62

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Query:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        MEAIEEDQSAKMDPP SP  CNP  PPLSKNARKKLLKQQRY+AKKAEKK Q KE KKKDIERKRKEWEETLASVTEEER+KLIESR+SLRKERMGKRSE
Subjt:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
        E+EKKIERLN AKDCGQ IVIDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
Subjt:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV

Query:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWKASFF+VVPQRKRCEA+ NS
Subjt:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHT
        DH VDGEENGD   DDE ETKKTKCDEVS +NHT
Subjt:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHT

XP_038893739.1 tRNA (guanine(9)-N1)-methyltransferase [Benincasa hispida]

1.7e-163

92.88

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Query:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        MEA+EEDQSAKMDPP SP A NP  PPLSKNA+KKLLKQQRY+AKKAEKKLQAKEQKKKDIERKRKEWE+TLASVTEEERSKLIESRRSLRKERMGKRSE
Subjt:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
        EREKKIERLN AKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
Subjt:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV

Query:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLTADAETVLDDLD KKIYIIGGLVDRNRWKGITMKKA EQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFF+VVPQRKRC ADSNS
Subjt:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDGEEN--GDGNGDDEHETKKTKCDEVSFTNHTL
        DH VD EEN  GDG+GDDEHE KKTKCDEVS ++ TL
Subjt:  DHQVDGEEN--GDGNGDDEHETKKTKCDEVSFTNHTL

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LYJ3 SAM-dependent MTase TRM10-type domain-containing protein

3.8e-153

87.76

Show/hide

Query:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        MEA+EE  + KMDPP S  ACNP  PPLSKNARKKLLKQQRY+AKKA+KKLQAKEQKKKDIERKRKEWEE LASVTEEER+KLIESRR+LRKERMGKRSE
Subjt:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
        +REKKIERL+ A+D GQNIVIDLEFSHLMTP EIHSLVQQIMYCYAVNGRCPSP HLWLTGCNGEMETQLQRLPGFDKWIIEKE RSYIDALQ+QKENLV
Subjt:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV

Query:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLTAD+ETVLDDLDLKKIYI+GGLVDRNRWKGITMKKAQEQ IQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWK SFFEVVPQRKRC  DSNS
Subjt:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHTL
        DH VDGEENGD  GD+++ETKKTKCDEVS +++ L
Subjt:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHTL

A0A6J1DYJ8 tRNA (guanine(9)-N(1))-methyltransferase

7.3e-152

88.86

Show/hide

Query:  MEAIEEDQSAKMDPPSSPPACNPP--PLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        MEA+E D  AKMDP S   AC PP  PLSKNARKKL KQQRY+AKKAEKK QAKEQKKKDIERKRKEWEE LASV+EEERSKLIESRRSLRKERMGKRSE
Subjt:  MEAIEEDQSAKMDPPSSPPACNPP--PLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
        EREKK+ERLN AKDCGQNIVIDLEFSHLMTPSEI+SLVQQIMYCYAVNGRC SPGHLWLTGCNGEMETQL RLPGFDKWIIEKEH+SYI+ L+DQKENLV
Subjt:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV

Query:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKYLETRDWKASFFEVVPQRKRCEADSNS
Subjt:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTN
        D  VD EENG+ +G  EHETKKTKCDE+S +N
Subjt:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTN

A0A6J1DZL7 tRNA (guanine(9)-N(1))-methyltransferase

7.3e-152

88.86

Show/hide

Query:  MEAIEEDQSAKMDPPSSPPACNPP--PLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        MEA+E D  AKMDP S   AC PP  PLSKNARKKL KQQRY+AKKAEKK QAKEQKKKDIERKRKEWEE LASV+EEERSKLIESRRSLRKERMGKRSE
Subjt:  MEAIEEDQSAKMDPPSSPPACNPP--PLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
        EREKK+ERLN AKDCGQNIVIDLEFSHLMTPSEI+SLVQQIMYCYAVNGRC SPGHLWLTGCNGEMETQL RLPGFDKWIIEKEH+SYI+ L+DQKENLV
Subjt:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV

Query:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKYLETRDWKASFFEVVPQRKRCEADSNS
Subjt:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTN
        D  VD EENG+ +G  EHETKKTKCDE+S +N
Subjt:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTN

A0A6J1EPG7 tRNA (guanine(9)-N(1))-methyltransferase

1.2e-159

90.42

Show/hide

Query:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        MEA EEDQSAKMDPP SP  CNP  PPLSKNARKKLLKQQRY+AKKAEKK Q KEQKKKDIERKRKEWEETLASVTEEER+KLIESR+SLRKERMGKRSE
Subjt:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
        E+EKKIERLN AKDCGQ IVIDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQD KENLV
Subjt:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV

Query:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITM KAQEQGIQTAKLPIGSYLKMSSSQVLTVNQV+EILL+Y+ETRDWKASFF+VVPQRKRCEA+ NS
Subjt:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHT
        DH VDG+ENGD   DDE ETKKTKCDEVS +NHT
Subjt:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHT

A0A6J1I7W5 tRNA (guanine(9)-N(1))-methyltransferase

2.5e-160

90.72

Show/hide

Query:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE
        MEAIEEDQSAKMDPP SP  CNP  PPLSKNARKKLLKQQRY+AKKAEKK Q KEQKKKDIERKRKEWE+TLASVTEEER+KLIESRRSLRKERMGKRSE
Subjt:  MEAIEEDQSAKMDPPSSPPACNP--PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSE

Query:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
        E+EKKIERLN AKDCGQ I+IDLEFS LMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV
Subjt:  EREKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLV

Query:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS
        YLTAD+ETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQV+EILLKY+ETRDWKASFF+VVPQRKRCEA+ NS
Subjt:  YLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNS

Query:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHT
        DH VDGEENGD   DDE E+KK KC EVS +NHT
Subjt:  DHQVDGEENGDGNGDDEHETKKTKCDEVSFTNHT

SwissProt top hits

e value

%identity

Alignment

O14214 tRNA (guanine(9)-N1)-methyltransferase

4.1e-35

Show/hide

Query:  PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIVIDLEF
        P LSK+A K+L +QQ + A + EK+ + + +KK+               + +EER + IE+   ++           +KK  RL         IV+D  F
Subjt:  PPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMAKDCGQNIVIDLEF

Query:  SHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQ-RLPG-FDKW-IIEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIG
          LM   EI+SL QQ+  C++ N     P  L+ T   G ++T+    L G  + W       +SY++  + QKE LVYL+AD++  + +LD  KIYIIG
Subjt:  SHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQ-RLPG-FDKW-IIEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIG

Query:  GLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNSDHQVDGEENGDGNGDDEHETKK
         +VD+NR+K +   KA EQGI+TAKLPI  Y+K++  ++LTVNQV EIL  +LE RDW+ +F EV+P+RK     S+    V  +     N  D    K+
Subjt:  GLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNSDHQVDGEENGDGNGDDEHETKK

Q3MHI8 tRNA methyltransferase 10 homolog A

2.5e-32

34.23

Show/hide

Query:  IEEDQSAKMDPPSSPPACNPPPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKK
        + ED      P SS       P+SK   KKL+KQ+++     E++ + ++QK+K+ +RKRK+ E      +  + S     RR +    +          
Subjt:  IEEDQSAKMDPPSSPPACNPPPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKK

Query:  IERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEKEHRSYIDALQDQKENLVY
                     ++ID  F  LM   +I  L +QI  CYA N R   P   +LT   G+++  + +   G+  W    I+ EH S       QKE+L+Y
Subjt:  IERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEKEHRSYIDALQDQKENLVY

Query:  LTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRK-------RC
        LT+D+  +L +LD  K Y+IGGLVD N  KG+T K+A + GI  A+LP+G+++KM+S +VL VN V EI+L+YLETRDW+ +FF ++PQRK        C
Subjt:  LTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRK-------RC

Query:  EADSNSDH--QVDGEENGDGNGDD-EHETKKTKCDE
        E+ S+     +V+G  N D + ++  HE   T  +E
Subjt:  EADSNSDH--QVDGEENGDGNGDD-EHETKKTKCDE

Q66JJ4 tRNA methyltransferase 10 homolog A

1.3e-33

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Query:  MEAIEEDQSAKMDPP-----SSPPACNPPPLSKNARKKLLKQQRYKAKKAEKKLQ---AKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKER
        +EA+ E     +DP      +S  A N  PL +N+           A ++E K Q   +K Q KK +  K+K+WE+              E R+  RKE+
Subjt:  MEAIEEDQSAKMDPP-----SSPPACNPPPLSKNARKKLLKQQRYKAKKAEKKLQ---AKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKER

Query:  MGKRSEEREKKIER----------LNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDK-WI---
          KR  ER+ + E            +  +     ++ID  F  LM   ++  L +QI  CYA N R   P  L+LT   G++++ +     +DK WI   
Subjt:  MGKRSEEREKKIER----------LNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDK-WI---

Query:  ---IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLET
           I+ EH  Y D +  +KE+LVYLT+D+  VL +LD  K YIIGGLVD N  KGIT KKA E GI  A+LP+G+++KM++ +VL VN V EI+L +LE 
Subjt:  ---IEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLET

Query:  RDWKASFFEVVPQRKRC--------EADSNSDHQVDGEENGDGNGDDEHET--KKTKCDE
        ++WK +FF V+PQRK          + +  +  Q DGE++   +  DE  T     +C+E
Subjt:  RDWKASFFEVVPQRKRC--------EADSNSDHQVDGEENGDGNGDDEHET--KKTKCDE

Q8C1Z8 tRNA methyltransferase 10 homolog A

5.4e-35

34.74

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Query:  EEDQSAKMDPPSSPPACNPPPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKI
        EE Q  ++D           P+SK   KKL+KQ++++ ++ ++K + KE++K                            R+ L + ++   S+  ++K 
Subjt:  EEDQSAKMDPPSSPPACNPPPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKI

Query:  ERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEKEHRSYIDALQDQKENLVYL
         R ++A+     +VID  F  LM   +I  L +QI  CYA N R   P   +LT   G+++  + +   G+  W    I+ EH S +     +KE+LVYL
Subjt:  ERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEKEHRSYIDALQDQKENLVYL

Query:  TADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQR-------KRCE
        T+D+  VL DLD  K Y+IGGLVD +  KG+T K+A   GI+ A+LP+  ++KM+S +VL VN V EI+L++LETRDW+ +FF ++PQR       K CE
Subjt:  TADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQR-------KRCE

Query:  ADSNSDHQ
        + S  DHQ
Subjt:  ADSNSDHQ

Q8TBZ6 tRNA methyltransferase 10 homolog A

2.8e-36

35.03

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Query:  EAIEEDQSAKMDPPSSPPACNPPPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEERE
        + I EDQ  +   P     C   P+SK   KKL+KQ+++     E++ + ++QK+K+ +RKRK+ E                     R+ +M   S+  +
Subjt:  EAIEEDQSAKMDPPSSPPACNPPPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEERE

Query:  KKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEKEHRSYIDALQDQKENL
        +K  R ++       ++ID  F HLM   +I  L +QI  CYA N R   P   +LT   G+++  + +   G+  W    I+ EH S +     +KE+L
Subjt:  KKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQL-QRLPGFDKW---IIEKEHRSYIDALQDQKENL

Query:  VYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQR-------K
        +YLT+D+  +L +LD  K Y+IGGLVD N  KG+T K+A + GI  A+LP+G+++KM+S +VL VN V EI+L+YLETRDW+ +FF ++PQR       K
Subjt:  VYLTADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQR-------K

Query:  RCEADSNSDHQVDGEENGDGNGDDEHETKKTKCD
         CE+ S+ +  V  EE G  +   E E  + + D
Subjt:  RCEADSNSDHQVDGEENGDGNGDDEHETKKTKCD

Arabidopsis top hits

e value

%identity

Alignment

AT5G47680.1 FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: tRNA (guanine-N1-)-methyltransferase (InterPro:IPR016009), tRNA (guanine-N(1)-)-methyltransferase, metazoa (InterPro:IPR016653), tRNA (guanine-N1-)-methyltransferase, eukaryotic (InterPro:IPR007356); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).

3.0e-110

67.56

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Query:  AIEEDQSAKMDPPSSPPA--CNPPPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEER
        A   D +   D P S PA     PPLSKNA+KK LKQQRY+AKKAEKK Q KE K+K+ ERK KEWEETLA+ TEEER KLIESR+SLRKERM KRSEE+
Subjt:  AIEEDQSAKMDPPSSPPA--CNPPPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEER

Query:  EKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLVYL
        EKKIERLN AK+ GQ IV+D++F+HLM+ SEI SLVQQIMYCYAVNGR  SP HLWLTG  G+M T+L +LPGF+KW IEKE R YI+A+ DQK+NLVYL
Subjt:  EKKIERLNMAKDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLVYL

Query:  TADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEAD-----
        TAD+ETVLDDLD K IYIIGGLVDRNR+KGITM KAQEQGI+TAKLPIG Y+KMSSSQVLTVNQV+EIL+K+LETRDWK +FF V+PQRKR   D     
Subjt:  TADAETVLDDLDLKKIYIIGGLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEAD-----

Query:  -----SNSDHQVDGEENGDGNGDDEHETKKTKCDEV
             S    + D E N   +  D  E KK  C EV
Subjt:  -----SNSDHQVDGEENGDGNGDDEHETKKTKCDEV

Sequences

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CDS sequence

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ATGGAGGCCATAGAAGAGGACCAGAGTGCTAAAATGGATCCTCCTTCTTCCCCTCCCGCCTGCAATCCTCCGCCCCTCTCCAAGAATGCCAGGAAGAAGCTACTGAAGCA
ACAACGATACAAAGCGAAGAAAGCGGAGAAGAAACTTCAGGCAAAAGAGCAGAAGAAGAAAGACATTGAGCGCAAACGGAAGGAATGGGAGGAGACACTGGCGAGTGTTA
CGGAGGAGGAGAGATCCAAGCTGATAGAGTCTCGGAGAAGCCTGAGGAAGGAGAGGATGGGGAAGAGGTCGGAGGAGAGGGAGAAGAAGATTGAGAGGCTCAACATGGCC
AAAGATTGTGGCCAAAATATTGTTATCGACCTTGAATTTTCTCACCTTATGACCCCTTCGGAGATTCACAGTCTTGTTCAGCAGATAATGTATTGTTATGCAGTGAATGG
AAGATGTCCTTCTCCTGGTCATCTCTGGTTGACTGGGTGTAATGGAGAAATGGAAACCCAACTACAAAGGCTTCCAGGATTTGATAAATGGATCATTGAGAAGGAACATA
GATCATATATAGATGCTTTGCAAGACCAGAAGGAAAATTTGGTATATCTCACAGCAGATGCAGAAACTGTGCTTGATGACCTTGATCTAAAGAAAATTTATATCATTGGT
GGGTTAGTTGATCGTAACAGATGGAAAGGGATTACCATGAAGAAAGCACAAGAACAGGGAATTCAAACAGCAAAACTTCCCATAGGAAGTTACTTGAAGATGTCTAGCTC
TCAGGTCCTTACTGTGAATCAAGTCATAGAGATTCTGCTCAAATATTTGGAAACAAGAGATTGGAAGGCTTCTTTCTTTGAGGTTGTTCCTCAAAGAAAAAGATGTGAAG
CTGATTCTAACAGTGACCATCAAGTAGATGGGGAAGAAAATGGAGATGGAAACGGAGATGATGAGCATGAGACGAAGAAGACCAAGTGTGATGAAGTATCTTTCACTAAC
CATACTTTGTAA

mRNA sequence

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Protein sequence

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MEAIEEDQSAKMDPPSSPPACNPPPLSKNARKKLLKQQRYKAKKAEKKLQAKEQKKKDIERKRKEWEETLASVTEEERSKLIESRRSLRKERMGKRSEEREKKIERLNMA
KDCGQNIVIDLEFSHLMTPSEIHSLVQQIMYCYAVNGRCPSPGHLWLTGCNGEMETQLQRLPGFDKWIIEKEHRSYIDALQDQKENLVYLTADAETVLDDLDLKKIYIIG
GLVDRNRWKGITMKKAQEQGIQTAKLPIGSYLKMSSSQVLTVNQVIEILLKYLETRDWKASFFEVVPQRKRCEADSNSDHQVDGEENGDGNGDDEHETKKTKCDEVSFTN
HTL

HG10009367 (gene) of Bottle gourd (Hangzhou Gourd) v1 genome