CuGenDBv2

Gene ID	HG10012996
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	EF-hand domain-containing protein
Genome location	Chr01:25991594..25998045
RNA-Seq Expression	HG10012996
Synteny	HG10012996
Gene Ontology terms	GO:0005509 - calcium ion binding (molecular function) GO:0048306 - calcium-dependent protein binding (molecular function)
InterPro domains	IPR002048 - EF-hand domain IPR011992 - EF-hand domain pair IPR018247 - EF-Hand 1, calcium-binding site

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0049066.1 uncharacterized protein E6C27_scaffold171G002950 [Cucumis melo var. makuwa]

6.4e-56

71.08

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Query:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS
        MGQAL R GG++W+++QL+ ISNKIFD+F    H++KLSFEDLYIATLLV+NDINKYLPGPH+DPPTKERV EL++ SD + ++ IDR+EFL FI  LT 
Subjt:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS

Query:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK
        E+  SVS RLI+TLVVAPT+AVVTK+STEG+PG+GK VQK+P+SAYA LVTLAAL FQNS QQLLK
Subjt:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK

KAG7028690.1 hypothetical protein SDJN02_09871, partial [Cucurbita argyrosperma subsp. argyrosperma]

1.1e-55

58.17

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Query:  ANEEKNVELRGGGGWHGTNFSYSRRLIYEVSDFQSPSAPSSMGQALGRIGGKEWRDEQLKKISNKIFDKF-KNQSHRDKLSFEDLYIATLLVYNDINKYL
        + +EK +          T+F++   L+YEV D QSPS PSSMGQ LGR GG+EWR ++LK IS++IF+K  KN+S  ++LSFE+LYIATLLV+NDINK  
Subjt:  ANEEKNVELRGGGGWHGTNFSYSRRLIYEVSDFQSPSAPSSMGQALGRIGGKEWRDEQLKKISNKIFDKF-KNQSHRDKLSFEDLYIATLLVYNDINKYL

Query:  PGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTSETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQ
         G HLDPP++E  K+LI   D + + +ID+ EF  F+  LTSETF SVS +L+LTLV+APT+AV TKK TEGVPGVGK VQKLP+SAYA ++TLAA+ FQ
Subjt:  PGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTSETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQ

Query:  NSQQQLLK
        +SQ  LLK
Subjt:  NSQQQLLK

XP_022146952.1 uncharacterized protein LOC111016024 [Momordica charantia]

2.6e-57

73.05

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Query:  SAPSSMGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFI
        S PS MGQ L +IGGK+WRD Q++KIS+KIF+K KN S +  LSFEDLYIATLLVYN+INK LPGPH DPP+KERV+ELI+ SD + D+EIDREEF+ FI
Subjt:  SAPSSMGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFI

Query:  LSLTSETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQ
        L LTS+T ++VS  L+LTLVVAPTVAVVTKKSTEGVPGVGK VQKLPAS YA L+TLAAL+FQN+ Q
Subjt:  LSLTSETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQ

XP_038876686.1 uncharacterized protein LOC120069050 [Benincasa hispida]

1.1e-76

92.77

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Query:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS
        MGQ+LGR GGKEWRDEQL+KISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKY+PGPHLDPPTKERVKELIQ SDRD DREIDREEFL+FILSLTS
Subjt:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS

Query:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK
        +TF+SVS RLILTLVVAPTVAVVTKKSTEGVPG+GKFVQKLPASAYAFLVTLAAL FQNSQQQLLK
Subjt:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK

XP_038892374.1 uncharacterized protein LOC120081499 [Benincasa hispida]

1.8e-58

76.61

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Query:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPT----KERVKELIQGSDRDNDREIDREEFLNFIL
        MGQAL   GGKEWRD+QL+KI+++IFDKF+ Q+H DKL  EDLYIATLLVYN+INK +PGPH DPPT    KERVKELIQ SD + D+ IDR EFL+FIL
Subjt:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPT----KERVKELIQGSDRDNDREIDREEFLNFIL

Query:  SLTSETFLSVSLRL-ILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK
        SLTSETF SVS RL ILTLVVAPTVAVVTKKSTE +PGVGK VQKLP SAYAFLVTLAAL FQ SQQQLL+
Subjt:  SLTSETFLSVSLRL-ILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK

TrEMBL top hits

e value

%identity

Alignment

A0A0A0L6I0 EF-hand domain-containing protein

1.5e-55

71.08

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Query:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS
        MGQA  R+GGKEWR+ QL+ ISNKIFD+F     +++LSFEDLYIATLLV+NDINKYLPG H+DPP K+RVKE+++  D++ +  IDREEFL F+  LTS
Subjt:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS

Query:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK
        E+F+SVS RLI+TLVVAPT+AVVTKKSTEGVPG+GK VQK+P+SAYA LVTLAAL FQNS+QQLLK
Subjt:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK

A0A1S3AVI1 uncharacterized protein LOC103483388

1.5e-55

70.48

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Query:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS
        MGQAL R  G++W+++QL+ ISNKIFD+F    H++KLSFEDLYIATLLV+NDINKYLPGPH+DPPTKERV EL++ SD++ ++ IDR+EFL FI  LT 
Subjt:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS

Query:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK
        E+  SVS RLI+TLVVAPT+AVVTK+STEG+PG+GK VQK+P+SAYA LVTLAAL FQNS QQLLK
Subjt:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK

A0A5A7U485 EF-hand domain-containing protein

3.1e-56

71.08

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Query:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS
        MGQAL R GG++W+++QL+ ISNKIFD+F    H++KLSFEDLYIATLLV+NDINKYLPGPH+DPPTKERV EL++ SD + ++ IDR+EFL FI  LT 
Subjt:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS

Query:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK
        E+  SVS RLI+TLVVAPT+AVVTK+STEG+PG+GK VQK+P+SAYA LVTLAAL FQNS QQLLK
Subjt:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK

A0A6J1D109 uncharacterized protein LOC111016024

1.3e-57

73.05

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Query:  SAPSSMGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFI
        S PS MGQ L +IGGK+WRD Q++KIS+KIF+K KN S +  LSFEDLYIATLLVYN+INK LPGPH DPP+KERV+ELI+ SD + D+EIDREEF+ FI
Subjt:  SAPSSMGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFI

Query:  LSLTSETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQ
        L LTS+T ++VS  L+LTLVVAPTVAVVTKKSTEGVPGVGK VQKLPAS YA L+TLAAL+FQN+ Q
Subjt:  LSLTSETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQ

A0A6J1EC16 uncharacterized protein LOC111431834

1.9e-53

69.88

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Query:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS
        M +ALGR GGKEWRDEQL+ IS++IFD   ++  +DKLSFEDLYIATLLVYNDINK LP  HLDPPT+ER +ELI+ +D + D++I R+EFL FILSLTS
Subjt:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS

Query:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK
        + F+S+S RLILTLV+APTVAVVTK+STEG+P +G  VQKLP+SAYAFLVTLAA  FQNSQ+ +LK
Subjt:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAALTFQNSQQQLLK

SwissProt top hits	e value	%identity	Alignment
No hits found

Arabidopsis top hits

e value

%identity

Alignment

AT1G64850.1 Calcium-binding EF hand family protein

1.9e-45

59.35

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Query:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS
        MGQ   ++ GKEWR +Q++ I +++FD+FK Q+ R  L+FE+LYIA LLVYNDINK LPGPH DPP+K+ V  ++   D + D E+DREEF+ FI  +T+
Subjt:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS

Query:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAAL
        ETF  VS  LI+ L+VAPTVA+ TKK+TEGVPGVGK VQKLP S YA LVTLA +
Subjt:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAAL

AT4G37445.1 BEST Arabidopsis thaliana protein match is: Calcium-binding EF hand family protein (TAIR:AT1G64850.1)

7.7e-07

23.87

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Query:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS
        MG ++G +G      + +  +   ++ +F  ++     +F+D ++A L ++N+ N  LPG H D PT +++K          D E  ++ F+ F+     
Subjt:  MGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLSFEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTS

Query:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAAL
         + L      ++T +V+P  A+  K++ E VP + K ++ +P   +   VT+ A+
Subjt:  ETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFLVTLAAL

Sequences

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CDS sequence

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ATGGCGTGCGGTAGCATCTGTGCAACATCGTCAGTGCGGAAGAAGGTTGAGGGGGGTTTGCGGCGTAGTGCAGTAGAAGGTTGTCTTCATGCGAATGAGGAGAAGAATGT
TGAGTTGAGGGGAGGGGGAGGGTGGCACGGCACCAATTTCAGTTACAGTCGGAGGCTGATTTACGAGGTCAGTGATTTTCAATCACCGTCTGCGCCGTCTAGCATGGGAC
AGGCATTGGGGAGAATCGGAGGCAAAGAATGGAGGGATGAGCAATTGAAGAAGATATCAAATAAAATATTTGATAAGTTTAAAAACCAATCGCATAGGGATAAGCTGTCG
TTTGAAGATCTGTACATTGCTACGTTACTTGTATACAATGATATTAATAAGTATTTGCCCGGACCGCATCTTGATCCTCCCACGAAGGAACGTGTCAAAGAACTCATACA
GGGCAGCGATAGGGACAATGACCGAGAAATTGATCGTGAAGAATTTTTGAATTTCATACTGAGCTTGACATCTGAGACGTTCCTATCAGTGAGCCTGAGATTGATCCTCA
CTTTGGTGGTAGCACCAACCGTCGCAGTAGTCACAAAGAAAAGTACTGAAGGCGTCCCAGGCGTTGGAAAATTTGTGCAAAAACTGCCCGCTTCAGCATATGCTTTCCTG
GTTACCCTTGCTGCTCTGACCTTTCAAAACTCTCAGCAGCAGCTTCTTAAATGA

mRNA sequence

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Protein sequence

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MACGSICATSSVRKKVEGGLRRSAVEGCLHANEEKNVELRGGGGWHGTNFSYSRRLIYEVSDFQSPSAPSSMGQALGRIGGKEWRDEQLKKISNKIFDKFKNQSHRDKLS
FEDLYIATLLVYNDINKYLPGPHLDPPTKERVKELIQGSDRDNDREIDREEFLNFILSLTSETFLSVSLRLILTLVVAPTVAVVTKKSTEGVPGVGKFVQKLPASAYAFL
VTLAALTFQNSQQQLLK

HG10012996 (gene) of Bottle gourd (Hangzhou Gourd) v1 genome