CuGenDBv2

Gene ID	HG10020625
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	DNA polymerase alpha subunit B
Genome location	Chr05:1045657..1050415
RNA-Seq Expression	HG10020625
Synteny	HG10020625
Gene Ontology terms	GO:0006259 - DNA metabolic process (biological process) GO:0006260 - DNA replication (biological process) GO:0005634 - nucleus (cellular component) GO:0003677 - DNA binding (molecular function)
InterPro domains	IPR007185 - DNA polymerase alpha/delta/epsilon, subunit B IPR013627 - DNA polymerase alpha, subunit B, N-terminal IPR016722 - DNA polymerase alpha, subunit B IPR043034 - DNA polymerase alpha, subunit B, N-terminal domain superfamily

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

KAE8652527.1 hypothetical protein Csa_013070, partial [Cucumis sativus]

1.2e-225

78.56

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Query:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE
        MEEEIGEEFK SGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNR LDGSVV++EQ+DGFLQHLQNEQKE VIKGEPDLHVYSSKDVDVILNDE
Subjt:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE

Query:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG
        +EDLN DILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKH TPFGQRTEKFVVRFNIN+MPD V A K DN ENDED IIKKVRPLQGC LTVHGSG
Subjt:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG

Query:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------
        FGLEPNCRFMYDKDEDRFNALDNRIMRHA ALAASGLYEEAVDPTVAS                                                    
Subjt:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------

Query:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI
        QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM                                   LGPF+
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI

Query:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG
        DSEHSDIKRGTVEMSFEEIF FEVLRRVQDYAEHMGPDARVLLMPSTRDA+HDF FPQPAFDIPSDL+LQISS PNPCILEANQ+KIACCTMDILRHLSG
Subjt:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG

Query:  EELSRNLTKGATNDRLSTLASHILRQR
        EELSRNLTKG TNDRLSTLASHILRQR
Subjt:  EELSRNLTKGATNDRLSTLASHILRQR

KAG7012749.1 DNA polymerase alpha subunit B [Cucurbita argyrosperma subsp. argyrosperma]

8.0e-217

74.44

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Query:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE
        M++EIGEEFK SGFTFDEEEEIL KCVTFCVNY+LKPSDIVSSWEVYYLNRQLDGS+VQI QMDGFLQHLQNEQKEA+IK EPDLHVYSSKDVD+ILNDE
Subjt:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE

Query:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG
        DED   DILKTPNGKSQ LHK+R DLTPYTNEGLFSSGKPSKH+TPFGQRTEKF VRF+IN++PDK  AE EDNCENDEDAIIKKV PLQGC LTVHGSG
Subjt:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG

Query:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------
        FGLEPNCRFMYD+ EDRFNAL+NRI++HA AL ASG YEE VDPTVAS                                                    
Subjt:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------

Query:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI
        QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIAL+HDIQPINQ ST SELSM                                   LGPF+
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI

Query:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG
        DSEHSDIKRGTV MSFEEIFNFEVLRRVQDY EHMGPDARVLLMPS RDA+HDFVFPQPAFDIPSDLRLQISSLPNP ILEANQ+KIACCTMDILRHLSG
Subjt:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG

Query:  EELSRNLTKGATNDRLSTLASHILRQRRHVLY
        EELSRNLTKG  NDRLSTLASHILRQRRHV +
Subjt:  EELSRNLTKGATNDRLSTLASHILRQRRHVLY

XP_004137446.1 DNA polymerase alpha subunit B [Cucumis sativus]

1.2e-225

78.56

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Query:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE
        MEEEIGEEFK SGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNR LDGSVV++EQ+DGFLQHLQNEQKE VIKGEPDLHVYSSKDVDVILNDE
Subjt:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE

Query:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG
        +EDLN DILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKH TPFGQRTEKFVVRFNIN+MPD V A K DN ENDED IIKKVRPLQGC LTVHGSG
Subjt:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG

Query:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------
        FGLEPNCRFMYDKDEDRFNALDNRIMRHA ALAASGLYEEAVDPTVAS                                                    
Subjt:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------

Query:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI
        QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM                                   LGPF+
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI

Query:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG
        DSEHSDIKRGTVEMSFEEIF FEVLRRVQDYAEHMGPDARVLLMPSTRDA+HDF FPQPAFDIPSDL+LQISS PNPCILEANQ+KIACCTMDILRHLSG
Subjt:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG

Query:  EELSRNLTKGATNDRLSTLASHILRQR
        EELSRNLTKG TNDRLSTLASHILRQR
Subjt:  EELSRNLTKGATNDRLSTLASHILRQR

XP_008452343.1 PREDICTED: DNA polymerase alpha subunit B [Cucumis melo]

2.7e-225

78.37

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Query:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE
        M EEIGEEFK SGFTFDEEEEILK+CVTFCVNYNLKPSDIVSSWEVYYLNR LDGSVV++EQMDGFLQHLQNEQKE VIKGEPDLHVYSSKDVDVILNDE
Subjt:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE

Query:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG
        +EDLN DILKTPNGKSQSLHKERLDLTPYTN GLFSSGKPSKH TPFGQRTEKFVVRFNINSMPD V AEKEDN ENDEDAIIKKVRPLQGC LTVHGSG
Subjt:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG

Query:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------
        FGLEPNCRFMYDKDEDRFNALDNRIMRHA ALAASGLYEEAVDPTVAS                                                    
Subjt:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------

Query:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI
        QVVGIEGHNPSGHCLIASK VDSFPLSATIDPILPPAKKIALNHD+QPINQSSTPSELSM                                   LGPF+
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI

Query:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG
        DSEHSDIKRGTVEMSFEEIF FEVLRRVQ+YAEHMGPDARVLLMPSTRDA+HDFVFPQP FDIPSDL+LQISS PNPCILEANQ+KIACCTMDILRHLSG
Subjt:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG

Query:  EELSRNLTKGATNDRLSTLASHILRQR
        EELSRNLTKG TNDRLSTLASHILRQR
Subjt:  EELSRNLTKGATNDRLSTLASHILRQR

XP_038886670.1 LOW QUALITY PROTEIN: DNA polymerase alpha subunit B [Benincasa hispida]

3.4e-215

75.76

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Query:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVI-LND
        MEE IGEE K SGFTFDEEEEILKKCVTFCVNYNLKPSDIV SWEVYYLNRQL GSVVQIE MDGFLQHLQNEQK+AVIKG+PDLHVYSSKDV +  LND
Subjt:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVI-LND

Query:  EDEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGS
        EDEDLNGDILKTPNGKSQSLHKERLD TPYTNEGLFS GKPS+H+TPFGQRTEKFVVRFNINS PDKV AEKEDNCENDEDAIIKKV+PLQGCLLT+HGS
Subjt:  EDEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGS

Query:  GFGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS---------------------------------------------------
        GFGLEPNCRFMYDKDEDRFNALDNRIMRHA ALAASGLYEEAVDPTVAS                                                   
Subjt:  GFGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS---------------------------------------------------

Query:  -QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPF
         QVVGIEGHNPSGHCLIASKL   F       PILPPAKKIALNHDIQPINQSSTPSELSM                                   LGPF
Subjt:  -QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPF

Query:  IDSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLS
        +DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHM PDARV+LMPS RDA+HDFVFPQPAFDIPSDLRLQISSLPNPCILEANQ+KIAC  MDILRHL 
Subjt:  IDSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLS

Query:  GEELSRNLTKGATNDRLSTLASHILRQR
        GEELSRNLTKG TNDRLSTLASHILRQR
Subjt:  GEELSRNLTKGATNDRLSTLASHILRQR

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LQA4 DNA polymerase alpha subunit B

6.0e-226

78.56

Show/hide

Query:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE
        MEEEIGEEFK SGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNR LDGSVV++EQ+DGFLQHLQNEQKE VIKGEPDLHVYSSKDVDVILNDE
Subjt:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE

Query:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG
        +EDLN DILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKH TPFGQRTEKFVVRFNIN+MPD V A K DN ENDED IIKKVRPLQGC LTVHGSG
Subjt:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG

Query:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------
        FGLEPNCRFMYDKDEDRFNALDNRIMRHA ALAASGLYEEAVDPTVAS                                                    
Subjt:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------

Query:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI
        QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM                                   LGPF+
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI

Query:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG
        DSEHSDIKRGTVEMSFEEIF FEVLRRVQDYAEHMGPDARVLLMPSTRDA+HDF FPQPAFDIPSDL+LQISS PNPCILEANQ+KIACCTMDILRHLSG
Subjt:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG

Query:  EELSRNLTKGATNDRLSTLASHILRQR
        EELSRNLTKG TNDRLSTLASHILRQR
Subjt:  EELSRNLTKGATNDRLSTLASHILRQR

A0A1S3BUF0 DNA polymerase alpha subunit B

1.3e-225

78.37

Show/hide

Query:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE
        M EEIGEEFK SGFTFDEEEEILK+CVTFCVNYNLKPSDIVSSWEVYYLNR LDGSVV++EQMDGFLQHLQNEQKE VIKGEPDLHVYSSKDVDVILNDE
Subjt:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE

Query:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG
        +EDLN DILKTPNGKSQSLHKERLDLTPYTN GLFSSGKPSKH TPFGQRTEKFVVRFNINSMPD V AEKEDN ENDEDAIIKKVRPLQGC LTVHGSG
Subjt:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG

Query:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------
        FGLEPNCRFMYDKDEDRFNALDNRIMRHA ALAASGLYEEAVDPTVAS                                                    
Subjt:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------

Query:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI
        QVVGIEGHNPSGHCLIASK VDSFPLSATIDPILPPAKKIALNHD+QPINQSSTPSELSM                                   LGPF+
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI

Query:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG
        DSEHSDIKRGTVEMSFEEIF FEVLRRVQ+YAEHMGPDARVLLMPSTRDA+HDFVFPQP FDIPSDL+LQISS PNPCILEANQ+KIACCTMDILRHLSG
Subjt:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG

Query:  EELSRNLTKGATNDRLSTLASHILRQR
        EELSRNLTKG TNDRLSTLASHILRQR
Subjt:  EELSRNLTKGATNDRLSTLASHILRQR

A0A5D3BJQ2 DNA polymerase alpha subunit B

2.3e-209

72.26

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Query:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVD------
        M EEIGEEFK SGFTFDEEEEILK+CVTFCVNYNLKPSDIVSSWEVYYLNR LDGSVV++EQMDGFLQHLQNEQKE VIKGEPDLHVYSSKDVD      
Subjt:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVD------

Query:  VILNDEDEDLNGDILKTPN---------------GKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDE
        V+L         + L+  N                  +SLHKERLDLTPYTN GLFSSGKPSKH TPFGQRTEKFVVRFNINSMPD V AEKEDN ENDE
Subjt:  VILNDEDEDLNGDILKTPN---------------GKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDE

Query:  DAIIKKVRPLQGCLLTVHGSGFGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS-------------------------------
        DAIIKKVRPLQGC LTVHGSGFGLEPNCRFMYDKDEDRFNALDNRIMRHA ALAASGLYEEAVDPTVAS                               
Subjt:  DAIIKKVRPLQGCLLTVHGSGFGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS-------------------------------

Query:  ---------------------QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-------------------
                             QVVGIEGHNPSGHCLIASK VDSFPLSATIDPILPPAKKIALNHD+QPINQSSTPSELSM                   
Subjt:  ---------------------QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-------------------

Query:  ----------------LGPFIDSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCI
                        LGPF+DSEHSDIKRGTVEMSFEEIF FEVLRRVQ+YAEHMGPDARVLLMPSTRDA+HDFVFPQP FDIPSDL+LQISS PNPCI
Subjt:  ----------------LGPFIDSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCI

Query:  LEANQSKIACCTMDILRHLSGEELSRNLTKGATNDRLSTLASHILRQR
        LEANQ+KIACCTMDILRHLSGEELSRNLTKG TNDRLSTLASHILRQR
Subjt:  LEANQSKIACCTMDILRHLSGEELSRNLTKGATNDRLSTLASHILRQR

A0A6J1FZE8 DNA polymerase alpha subunit B

1.6e-215

74.76

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Query:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE
        M++EIGEEFK SGFTFDEEEEIL KCVTFCVNY+LKPSDIVSSWEVYYLNRQLDGS+VQI QMDGFLQHLQNEQKEA+IK EPDLHVYSSKDVD+ILNDE
Subjt:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE

Query:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG
        DED   DILKTPNGKSQ LHK+R DLTPYTNEGLFSSGKPSKH+TPFGQRTEKF VRF+IN++PDK  AE EDNCENDEDAIIKKV PLQGC LTVHGSG
Subjt:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG

Query:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------
        FGLEPNCRFMYD+ EDRFNAL+NRI++HA AL ASGLYEE VDPTVAS                                                    
Subjt:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------

Query:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI
        QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKI L+HDIQPINQ ST SELSM                                   LGPF+
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI

Query:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG
        DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDY EHMGPDARVLLMPS RDA+HDFVFPQPAFDIPSDLRLQISSLPNP ILEANQ+KIACCTMDILRHLSG
Subjt:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG

Query:  EELSRNLTKGATNDRLSTLASHILRQR
        EELSRNLTKG  NDRLSTLASHILRQR
Subjt:  EELSRNLTKGATNDRLSTLASHILRQR

A0A6J1HSI8 DNA polymerase alpha subunit B

3.4e-213

74.38

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Query:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE
        M++EIGEEFK SGFTFDEEEEILKKCVTFC+NY+LKPSDIVSSWEVYYLNRQLDGS+VQI QMDGFLQHLQNEQKEA+IK EPDLHVYSSKDVD+ILNDE
Subjt:  MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDE

Query:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG
        DED   DILKTPNGKSQ LHK+R DLTPYTNEGLFSSGKPSKH+TPFGQRTEKF VRF+IN++PDK  AE EDNCENDEDAIIKKV PLQGC LTVHGSG
Subjt:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG

Query:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------
        FGLEPNCRFMYD+ EDRFNAL+NRI++HA AL ASGLYEE VDPTVAS                                                    
Subjt:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------

Query:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI
        QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIAL+HDIQPI  SST SELSM                                   LGPF+
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSM-----------------------------------LGPFI

Query:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG
        DSEHSDIKRGTVEMSFEEIF FEVLRRVQDY EHMGPDARVLLMPS RDA+HDFVFPQPAFDIPSDLRLQ+SSLPNP ILEANQ+KIACCTMDILRHLSG
Subjt:  DSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSG

Query:  EELSRNLTKGATNDRLSTLASHILRQR
        EELSRNLTKG  NDRLSTLASHILRQR
Subjt:  EELSRNLTKGATNDRLSTLASHILRQR

SwissProt top hits

e value

%identity

Alignment

O74946 DNA polymerase alpha subunit B

8.8e-09

29.46

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Query:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPIN-------------------------QSSTPSELSML-GPFIDSEHSDIK-
        Q+V ++G NPSG+  IA +++   PL          A  +A N + QPI+                          +  P +L +L GPF+D  H  I+ 
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPIN-------------------------QSSTPSELSML-GPFIDSEHSDIK-

Query:  ---RGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDF-VFPQPAFD-IPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSGEEL
            GT   S EE+F      RV      +      +L+P   DA  D   +PQ AF+ +   L       PNPC+   N       T DIL H S EEL
Subjt:  ---RGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDF-VFPQPAFD-IPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSGEEL

Query:  SRNLTKGATNDRLSTLASHILRQR
         R  + G   +  + L SH+L QR
Subjt:  SRNLTKGATNDRLSTLASHILRQR

O89043 DNA polymerase alpha subunit B

5.2e-17

29.33

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Query:  QVVGIEGHNPSGHCLIASKLVDSFPL----------------------------SATIDPILPPAKKIALNHDIQPINQSSTPSELSMLGPFIDSEHSDI
        QVV +EG N +G  L A+KL +  PL                            S T DP+L     I  NHD         P    + GPF+D++H  +
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPL----------------------------SATIDPILPPAKKIALNHDIQPINQSSTPSELSMLGPFIDSEHSDI

Query:  KRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAF---DIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSGEELS
        +   +   FE+IF  + LR V +     G  + ++ +PS RD HH+ V+PQP F   ++P + + ++  +  PC L  N       + D+L H+  EE+ 
Subjt:  KRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAF---DIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSGEELS

Query:  RNLTKGATNDRLSTLASHILRQRRH
           +   T+DR S +  HIL QR +
Subjt:  RNLTKGATNDRLSTLASHILRQRRH

P33611 DNA polymerase alpha subunit B

9.7e-16

27.56

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Query:  QVVGIEGHNPSGHCLIASKLVDSFPL----------------------------SATIDPILPPAKKIALNHDIQPINQSSTPSELSMLGPFIDSEHSDI
        QVV +EG N +G  L A+KL +  PL                            S T DP+L          D+  I     P    + GPF+D++H  +
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPL----------------------------SATIDPILPPAKKIALNHDIQPINQSSTPSELSMLGPFIDSEHSDI

Query:  KRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAF---DIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSGEELS
        +   +   FE++F  + LR V +     G  + ++ +PS RD HH+ V+PQP F   ++  + + ++  +  PC L  N       + D+L H+  EE+ 
Subjt:  KRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAF---DIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSGEELS

Query:  RNLTKGATNDRLSTLASHILRQRRH
           +   T+DR S +  HIL QR +
Subjt:  RNLTKGATNDRLSTLASHILRQRRH

Q14181 DNA polymerase alpha subunit B

1.4e-17

29.78

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Query:  QVVGIEGHNPSGHCLIASKLVDSFPL----------------------------SATIDPILPPAKKIALNHDIQPINQSSTPSELSMLGPFIDSEHSDI
        QVV +EG N +G  L+A+KL +  PL                            S T DP+L       +NHD         P    + GPF+D++H  +
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPL----------------------------SATIDPILPPAKKIALNHDIQPINQSSTPSELSMLGPFIDSEHSDI

Query:  KRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAF---DIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSGEELS
        +   +   FE+IF  + LR + +     G  + ++ +PS RD HH+ V+PQP F   D+  + + Q+  +  PC L  N       + D+L HL  EE+S
Subjt:  KRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAF---DIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSGEELS

Query:  RNLTKGATNDRLSTLASHILRQRRH
           +   T+DR S +  HIL QR +
Subjt:  RNLTKGATNDRLSTLASHILRQRRH

Q58D13 DNA polymerase alpha subunit B

5.2e-17

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Query:  QVVGIEGHNPSGHCLIASKLVDSFPL----------------------------SATIDPILPPAKKIALNHDIQPINQSSTPSELSMLGPFIDSEHSDI
        QVV +EG N +G  L+A++L +  PL                            S T DP+L          D+  I     P    + GPF+D++H  +
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPL----------------------------SATIDPILPPAKKIALNHDIQPINQSSTPSELSMLGPFIDSEHSDI

Query:  KRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAF---DIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSGEELS
        +   +  SFE++F  + LR + +     G  + ++++PS RD HH+ V+PQP F   D+  + + ++  +  PC L  N       + D+L H+  EE+S
Subjt:  KRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAF---DIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSGEELS

Query:  RNLTKGATNDRLSTLASHILRQRRH
           +   T+DR S +  HIL QR +
Subjt:  RNLTKGATNDRLSTLASHILRQRRH

Arabidopsis top hits

e value

%identity

Alignment

AT1G67630.1 DNA polymerase alpha 2

2.5e-123

46.88

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Query:  EEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIK-GEPDLHVYSSKDVDVILNDE
        +EEI  EFK SGF   EE+EILK+CVT C+NY+LKPSD+VSSWE+Y+LNRQL    V+ + MDGF+ HLQNEQKE++++  E  LH+YS++DVD++L+  
Subjt:  EEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIK-GEPDLHVYSSKDVDVILNDE

Query:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG
         ED   +I+ TP  KSQ LH +  D    + +  +S+GK   H+TPFG R EKFVV+FN+ ++         ++ EN ED IIK+V+  Q C L V+GS 
Subjt:  DEDLNGDILKTPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSG

Query:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------
         G EP CRFMYD+ EDRF+AL+ RI+RHA A AASGLYEE VDP VAS                                                    
Subjt:  FGLEPNCRFMYDKDEDRFNALDNRIMRHAYALAASGLYEEAVDPTVAS----------------------------------------------------

Query:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDI------------------------------QPINQ------SSTPSELSMLGPF
        QVVGIEG NPSGH L ASKL+DS PL+ T+D  LPPAK+  L+ ++                              +P+N+         P  L +LGPF
Subjt:  QVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDI------------------------------QPINQ------SSTPSELSMLGPF

Query:  IDSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIP-SDLRLQISSLPNPCILEANQSKIACCTMDILRHL
        +DSEH +IK+G V+ +F EIF  EVLR++QDY E MG + RV+L+PS RDA+HDF+FPQP FDI   DL  Q++SL NP   EANQ K++CCT+D+L+ L
Subjt:  IDSEHSDIKRGTVEMSFEEIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIP-SDLRLQISSLPNPCILEANQSKIACCTMDILRHL

Query:  SGEELSRNLTKGATNDRLSTLASHILRQR
        SGEE+S+N   G   DR+S LASH+LRQR
Subjt:  SGEELSRNLTKGATNDRLSTLASHILRQR

Sequences

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CDS sequence

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ATGGAAGAAGAGATCGGAGAAGAATTCAAGTGGAGTGGTTTCACGTTTGATGAAGAGGAAGAGATTCTCAAGAAATGTGTTACGTTCTGTGTTAATTACAATCTAAAGCC
TTCCGATATTGTTTCTAGCTGGGAGGTCTACTATCTCAACAGGCAATTAGATGGGTCAGTGGTGCAAATTGAACAGATGGATGGGTTCTTACAACATTTACAGAATGAGC
AAAAAGAAGCAGTCATTAAAGGGGAACCAGATCTTCATGTTTACTCGAGCAAAGATGTTGACGTGATTTTGAATGATGAGGATGAAGATCTAAATGGAGACATTCTTAAG
ACCCCAAATGGGAAATCTCAGAGCCTCCACAAAGAGCGATTGGATTTGACACCTTACACGAATGAAGGCTTGTTCTCATCGGGAAAGCCTTCAAAGCATATGACACCCTT
TGGTCAAAGAACAGAAAAGTTTGTTGTGAGGTTCAACATTAATAGCATGCCTGATAAGGTGGCTGCTGAAAAGGAAGATAATTGCGAGAATGATGAAGATGCAATCATCA
AGAAGGTTCGACCCCTGCAAGGATGCTTATTGACAGTCCATGGATCAGGATTTGGACTTGAACCAAATTGCAGGTTCATGTATGACAAGGATGAAGATAGGTTTAATGCT
CTTGATAACCGAATCATGAGGCATGCTTATGCTCTTGCTGCATCTGGGCTCTACGAAGAAGCAGTGGACCCTACAGTTGCTTCACAGGTAGTAGGTATTGAAGGGCATAA
TCCTAGTGGACACTGCTTGATTGCGTCAAAACTTGTAGATTCTTTTCCTCTGTCCGCCACTATAGACCCCATTCTGCCTCCAGCAAAGAAGATAGCTTTGAACCATGATA
TCCAACCAATTAATCAATCTTCTACACCGTCAGAATTATCGATGCTTGGACCATTTATTGATTCTGAACATTCTGATATCAAGAGAGGAACTGTAGAGATGAGCTTTGAG
GAAATTTTCAACTTTGAAGTTTTAAGAAGGGTACAAGATTATGCAGAACACATGGGTCCTGATGCACGTGTACTGTTGATGCCATCTACAAGGGATGCTCATCACGACTT
TGTTTTTCCTCAGCCTGCTTTTGACATTCCATCTGATCTTAGACTTCAGATATCCAGTCTCCCAAACCCATGCATTTTGGAAGCAAATCAGTCAAAGATAGCCTGCTGCA
CCATGGATATTCTCAGACACCTTAGTGGAGAAGAGCTGTCAAGAAACCTAACTAAGGGAGCAACCAACGACCGCCTGAGCACACTTGCAAGTCATATACTTCGCCAGCGC
AGGCATGTTTTATATTAA

mRNA sequence

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Protein sequence

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MEEEIGEEFKWSGFTFDEEEEILKKCVTFCVNYNLKPSDIVSSWEVYYLNRQLDGSVVQIEQMDGFLQHLQNEQKEAVIKGEPDLHVYSSKDVDVILNDEDEDLNGDILK
TPNGKSQSLHKERLDLTPYTNEGLFSSGKPSKHMTPFGQRTEKFVVRFNINSMPDKVAAEKEDNCENDEDAIIKKVRPLQGCLLTVHGSGFGLEPNCRFMYDKDEDRFNA
LDNRIMRHAYALAASGLYEEAVDPTVASQVVGIEGHNPSGHCLIASKLVDSFPLSATIDPILPPAKKIALNHDIQPINQSSTPSELSMLGPFIDSEHSDIKRGTVEMSFE
EIFNFEVLRRVQDYAEHMGPDARVLLMPSTRDAHHDFVFPQPAFDIPSDLRLQISSLPNPCILEANQSKIACCTMDILRHLSGEELSRNLTKGATNDRLSTLASHILRQR
RHVLY

HG10020625 (gene) of Bottle gourd (Hangzhou Gourd) v1 genome