CuGenDBv2

Gene ID	HG10023206
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	pre-mRNA-splicing factor ISY1 homolog
Genome location	Chr05:32194959..32195870
RNA-Seq Expression	HG10023206
Synteny	HG10023206
Gene Ontology terms	GO:0000350 - generation of catalytic spliceosome for second transesterification step (biological process) GO:0000389 - mRNA 3'-splice site recognition (biological process) GO:0000974 - Prp19 complex (cellular component) GO:0071013 - catalytic step 2 spliceosome (cellular component) GO:0071014 - post-mRNA release spliceosomal complex (cellular component) GO:0071020 - post-spliceosomal complex (cellular component)
InterPro domains	IPR009360 - Pre-mRNA-splicing factor Isy1 IPR029012 - Helix hairpin bin domain superfamily IPR037200 - Pre-mRNA-splicing factor Isy1 superfamily

Homology

Show/hide homology

GenBank top hits

e value

%identity

Alignment

XP_008461190.1 PREDICTED: pre-mRNA-splicing factor ISY1 homolog [Cucumis melo]

3.4e-158

99.34

Show/hide

Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
        TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAE VEEWQRME
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
        EIRKEAKRAVKSGEVASVVNVAAK VLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN

Query:  IQR
        IQR
Subjt:  IQR

XP_011659495.1 pre-mRNA-splicing factor ISY1 homolog [Cucumis sativus]

7.5e-158

99.01

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Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
        TKHSAKMTDL+GNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAE VEEWQRME
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
        EIRKEAKRAVKSGEVASVVNVAAK VLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN

Query:  IQR
        IQR
Subjt:  IQR

XP_022152151.1 pre-mRNA-splicing factor ISY1 homolog [Momordica charantia]

1.4e-156

97.69

Show/hide

Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNY
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
        TKHSAKMTDL+GNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMR E VEEWQRME
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
        EIRKEAKRAVKSGEVASVVNVAAK VLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLL+EQSEAK+MLN
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN

Query:  IQR
        IQR
Subjt:  IQR

XP_022960451.1 pre-mRNA-splicing factor ISY1 homolog [Cucurbita moschata]

9.1e-156

97.36

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Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNY
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
        +K+SAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEER+RAE VEEWQRME
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
        EIRKEAKRAVKSGEVASVVNVAAK VLFEEEEEVVEEER+REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAK+MLN
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN

Query:  IQR
        IQR
Subjt:  IQR

XP_023004198.1 pre-mRNA-splicing factor ISY1 homolog [Cucurbita maxima]

3.1e-156

97.69

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Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNY
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
        TK+SAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEER+RAE VEEWQRME
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
        EIRKEAKRAVKSGEVASVVNVAAK VLFEEEEEVVEEER+REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAK+MLN
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN

Query:  IQR
        IQR
Subjt:  IQR

TrEMBL top hits

e value

%identity

Alignment

A0A0A0KBN6 Uncharacterized protein

3.6e-158

99.01

Show/hide

Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
        TKHSAKMTDL+GNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAE VEEWQRME
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
        EIRKEAKRAVKSGEVASVVNVAAK VLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN

Query:  IQR
        IQR
Subjt:  IQR

A0A1S3CE48 pre-mRNA-splicing factor ISY1 homolog

1.6e-158

99.34

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Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
        TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAE VEEWQRME
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
        EIRKEAKRAVKSGEVASVVNVAAK VLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN

Query:  IQR
        IQR
Subjt:  IQR

A0A5D3CH05 Pre-mRNA-splicing factor ISY1-like protein

1.6e-158

99.34

Show/hide

Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
        TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAE VEEWQRME
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
        EIRKEAKRAVKSGEVASVVNVAAK VLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN

Query:  IQR
        IQR
Subjt:  IQR

A0A6J1DGR8 pre-mRNA-splicing factor ISY1 homolog

6.8e-157

97.69

Show/hide

Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNY
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
        TKHSAKMTDL+GNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMR E VEEWQRME
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
        EIRKEAKRAVKSGEVASVVNVAAK VLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLL+EQSEAK+MLN
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN

Query:  IQR
        IQR
Subjt:  IQR

A0A6J1KYR8 pre-mRNA-splicing factor ISY1 homolog

1.5e-156

97.69

Show/hide

Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRI+ELGGPNY
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
        TK+SAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEER+RAE VEEWQRME
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
        EIRKEAKRAVKSGEVASVVNVAAK VLFEEEEEVVEEER+REREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAK+MLN
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN

Query:  IQR
        IQR
Subjt:  IQR

SwissProt top hits

e value

%identity

Alignment

Q20716 Protein isy-1

1.2e-44

42.25

Show/hide

Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARN EKA + L R+  +K EE++ P  RRP+   +CR+L++A+++R++I+R+  +K+  IQN GLGE +LRDLNDE+N+LI+ K  WE+RI ELGG +Y
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPE-LRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRM
         K++ K  D  G        +G   GY+YFGAAK LPGVRELFEK  E   +RR R D+ + IDA Y+GY DDEDG L  +E   EE+           +
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPE-LRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRM

Query:  EEIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKY
        E I KE   A K  +     + AA   +++ EE+  +++ + +       D     + HV LP +++IE+M+LE+KK EL++KY
Subjt:  EEIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKY

Q54N41 Pre-mRNA-splicing factor ISY1 homolog

1.0e-53

42.9

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Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARNEEKA+SMLNR++ LK  E K+ +ERRPYL++EC  L +A+KWR+QI++EI R + EIQN  L E+++RDLND INKL+REK HWERRI++LGGPN+
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPEL---RKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQ
           + K+ D +G       P G G  YRY+G AK LPGV ELFEKP +     +++TR D+YK ID+ YYGYRDDEDG LE +E   E+    + VE W+
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPEL---RKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQ

Query:  RMEEIR-------------KEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKERE--------FVVHVPLPDEKEIEKMVLEKKK
        + +  +             K +    K+ E    +N+       EE ++          E  EN++  +E        F  +V LP   +IE ++LEK+K
Subjt:  RMEEIR-------------KEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKERE--------FVVHVPLPDEKEIEKMVLEKKK

Query:  MELLSKYASD
         EL  +Y  +
Subjt:  MELLSKYASD

Q69ZQ2 Pre-mRNA-splicing factor ISY1 homolog

1.3e-64

51.17

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Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARN EKA + L RF   + EE  K KERRP+LASEC +L +A+KWR+QI+ EI +KVA+IQN GLGE R+RDLNDEINKL+REK HWE RI ELGGP+Y
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
         K   KM D EG  V    P  R  GY+YFGAAK LPGVRELFEK P    R+TR ++ K ID  YYGY D++DGV+  +E   E+++RAE VE+W+   
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSML
        E R    R  K  E      +   AV  EE +E   +E+  E       D +++F+ HVP+P ++EIE+ ++ +KKMELL KYAS+ L  +  EAK +L
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSML

Q6AYB3 Pre-mRNA-splicing factor ISY1 homolog

1.7e-64

51.51

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Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARN EKA + L RF   + EE  K KERRP+LASEC +L +A+KWR+QI+ EI +KVA+IQN GLGE R+RDLNDEINKL+REK HWE RI ELGGP+Y
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
         K   KM D EG  V    P  R  GY+YFGAAK LPGVRELFEK P    R+TR ++ K ID  YYGY D++DGV+  +E   E+R+RAE VE+W+   
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSML
        E R  A+   +  E    +N+   AV  EE +E   +E+  E       D +++F+ HVP+P ++EIE+ ++ +KKMELL KYAS+ L  +  EAK +L
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSML

Q9ULR0 Pre-mRNA-splicing factor ISY1 homolog

5.0e-64

50.5

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Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARN EKA + L RF   + EE  K KERRP+LASEC +L +A+KWR+QI+ EI +KVA+IQN GLGE R+RDLNDEINKL+REK HWE RI ELGGP+Y
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
         K   KM D EG  V    P  R  GY+YFGAAK LPGVRELFEK P    R+TR ++ K ID  YYGY D++DGV+  +E   E+++RAE VE+W+   
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSML
        E R       +  E    +N+ A        EE  +EE  +E   K   D +++F+ HVP+P ++EIE+ ++ +KKMELL KYAS+ L  +  EA+ +L
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSML

Arabidopsis top hits

e value

%identity

Alignment

AT3G18790.1 FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Isy1-like splicing (InterPro:IPR009360); Has 1147 Blast hits to 965 proteins in 236 species: Archae - 12; Bacteria - 13; Metazoa - 351; Fungi - 230; Plants - 49; Viruses - 9; Other Eukaryotes - 483 (source: NCBI BLink).

2.0e-129

80.2

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Query:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY
        MARNEEKAQSMLNRFI  K  EKKKPKERRPYLASECRDLAEADKWRQQI+REIG KVAEIQNEGLGEHRLRDLNDEINKL+RE+ HWERRI+ELGG NY
Subjt:  MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNY

Query:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME
        +KHSAKMTDLEGNI+DVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKR+TRYDIYKRIDASYYGYRDDEDG+LE++E  SE  MR  +VEEW+R++
Subjt:  TKHSAKMTDLEGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRME

Query:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN
        E+RKEA++             AA+ VLFEEEE+VVEEER  ERE +E  +KEREFVVHVPLPDEKEIEKMVLEKKKM+LLSKYAS+ L+E+Q+EAKSMLN
Subjt:  EIRKEAKRAVKSGEVASVVNVAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLN

Query:  IQR
        I R
Subjt:  IQR

Sequences

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CDS sequence

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ATGGCTCGAAATGAGGAGAAAGCACAGTCGATGCTAAATCGTTTCATAGCCTTAAAAGCTGAGGAAAAGAAGAAACCGAAGGAACGCCGACCCTACCTTGCCTCTGAGTG
TCGTGACCTTGCAGAAGCTGACAAATGGCGTCAGCAAATCATGCGTGAGATTGGTCGTAAGGTTGCAGAGATTCAAAATGAAGGCCTTGGTGAGCATCGTTTGCGCGACC
TCAACGATGAGATTAACAAGCTCATTAGAGAGAAGTCACATTGGGAGCGTCGTATAATTGAGCTTGGTGGCCCCAATTATACCAAACACTCGGCCAAAATGACTGATTTA
GAGGGGAATATTGTTGATGTACCAAACCCCAGTGGTCGTGGTCCTGGATATCGGTATTTTGGTGCTGCTAAGAAGTTACCGGGTGTTAGGGAATTGTTTGAGAAGCCACC
TGAACTGCGGAAGCGCAGGACTCGTTATGACATATACAAGAGGATTGACGCAAGCTACTATGGCTACAGGGACGATGAGGATGGGGTTTTGGAAAGAGTAGAAGGTCCAA
GTGAAGAGCGTATGAGAGCTGAGACAGTTGAAGAGTGGCAAAGAATGGAAGAGATAAGGAAAGAAGCGAAGAGGGCAGTAAAGAGCGGGGAGGTGGCCAGCGTGGTGAAT
GTTGCAGCAAAGGCGGTACTATTTGAAGAGGAGGAAGAGGTTGTTGAAGAAGAGAGGAAGAGAGAGAGGGAAATGAAGGAGAATTTGGACAAGGAGAGGGAGTTTGTTGT
GCATGTGCCATTACCTGATGAAAAGGAGATTGAGAAGATGGTATTGGAGAAGAAGAAGATGGAACTGTTAAGCAAGTATGCAAGTGATATGCTTTTAGAGGAACAGAGTG
AGGCCAAGTCCATGCTCAATATACAGAGGTAG

mRNA sequence

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Protein sequence

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MARNEEKAQSMLNRFIALKAEEKKKPKERRPYLASECRDLAEADKWRQQIMREIGRKVAEIQNEGLGEHRLRDLNDEINKLIREKSHWERRIIELGGPNYTKHSAKMTDL
EGNIVDVPNPSGRGPGYRYFGAAKKLPGVRELFEKPPELRKRRTRYDIYKRIDASYYGYRDDEDGVLERVEGPSEERMRAETVEEWQRMEEIRKEAKRAVKSGEVASVVN
VAAKAVLFEEEEEVVEEERKREREMKENLDKEREFVVHVPLPDEKEIEKMVLEKKKMELLSKYASDMLLEEQSEAKSMLNIQR

HG10023206 (gene) of Bottle gourd (Hangzhou Gourd) v1 genome