CuGenDBv2

Gene ID	IVF0003750
Organism	Cucumis melo ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	mediator of RNA polymerase II transcription subunit 32
Genome location	chr11:4170536..4170974
RNA-Seq Expression	IVF0003750
Synteny	IVF0003750
Gene Ontology terms	GO:0006355 - regulation of transcription, DNA-templated (biological process) GO:0009631 - cold acclimation (biological process) GO:0010150 - leaf senescence (biological process) GO:0048364 - root development (biological process) GO:0016592 - mediator complex (cellular component)
InterPro domains	IPR033244 - Mediator of RNA polymerase II transcription subunit 32

Homology

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GenBank top hits

e value

%identity

Alignment

KAA0045925.1 mediator of RNA polymerase II transcription subunit 32 [Cucumis melo var. makuwa]

6.30e-80

89.04

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANE               NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTED TQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ

TYK13665.1 mediator of RNA polymerase II transcription subunit 32 [Cucumis melo var. makuwa]

1.09e-80

89.73

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANE               NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ

XP_004148855.1 mediator of RNA polymerase II transcription subunit 32 [Cucumis sativus]

3.72e-78

87.67

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANE               NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQS TT LPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTED TQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ

XP_008457936.1 PREDICTED: mediator of RNA polymerase II transcription subunit 32 [Cucumis melo]

9.52e-81

89.73

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANE               NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ

XP_038902813.1 mediator of RNA polymerase II transcription subunit 32 [Benincasa hispida]

2.62e-78

86.99

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Query:  MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEANE               NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQ+ TTG+PPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEANE---------------NFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTED TQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ

TrEMBL top hits

e value

%identity

Alignment

A0A0A0LIN7 Uncharacterized protein

2.8e-59

87.67

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Query:  MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEAN               ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQS TT LPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTED TQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ

A0A1S3C6T6 mediator of RNA polymerase II transcription subunit 32

3.0e-61

89.73

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Query:  MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEAN               ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ

A0A5A7TWZ7 Mediator of RNA polymerase II transcription subunit 32

1.2e-60

89.04

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Query:  MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEAN               ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTED TQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ

A0A5D3CQ46 Mediator of RNA polymerase II transcription subunit 32

3.0e-61

89.73

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Query:  MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
        MDSLVDSLNNAYQEFVAGAANVLEAN               ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAV

Query:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
        RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
Subjt:  RLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ

A0A6J1JH28 mediator of RNA polymerase II transcription subunit 32-like isoform X2

1.5e-55

83.89

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Query:  MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSG--TTGLPPIS
        MDSLVDSLNNAYQEFV+GAANVLEAN               ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQ+   TTGLPPIS
Subjt:  MDSLVDSLNNAYQEFVAGAANVLEAN---------------ENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSG--TTGLPPIS

Query:  AVRLEQMSKAVRWLVIELQHGSG-AAASASAHSHQSAPFDARFTEDTTQ
        A+RLEQMSKAVRWLVIELQHGSG AAASASAHSH SAPFDARFTED TQ
Subjt:  AVRLEQMSKAVRWLVIELQHGSG-AAASASAHSHQSAPFDARFTEDTTQ

SwissProt top hits

e value

%identity

Alignment

Q84VW5 Mediator of RNA polymerase II transcription subunit 32

1.6e-38

62.75

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Query:  MDSLVDSLNNAYQEFVAGAANVLE---------------ANENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATG---SLAGKSGQSGTTG----
        MD++VDSLN AY++FV  +A VLE               A ENFK+KWELFRVACDQAEEFVESVKQRIGSECLVDEATG   + AG    +  TG    
Subjt:  MDSLVDSLNNAYQEFVAGAANVLE---------------ANENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATG---SLAGKSGQSGTTG----

Query:  LPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
        LPPISAVRLEQMS+AVRWLV+ELQ GSG  A  S HS  S  FD+RF+ED+TQ
Subjt:  LPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ

Arabidopsis top hits

e value

%identity

Alignment

AT1G11760.1 MED32; Has 41 Blast hits to 40 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 41; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink).

1.1e-39

62.75

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Query:  MDSLVDSLNNAYQEFVAGAANVLE---------------ANENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATG---SLAGKSGQSGTTG----
        MD++VDSLN AY++FV  +A VLE               A ENFK+KWELFRVACDQAEEFVESVKQRIGSECLVDEATG   + AG    +  TG    
Subjt:  MDSLVDSLNNAYQEFVAGAANVLE---------------ANENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATG---SLAGKSGQSGTTG----

Query:  LPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ
        LPPISAVRLEQMS+AVRWLV+ELQ GSG  A  S HS  S  FD+RF+ED+TQ
Subjt:  LPPISAVRLEQMSKAVRWLVIELQHGSGAAASASAHSHQSAPFDARFTEDTTQ

Sequences

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CDS sequence

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ATGGACAGCCTAGTGGACTCTTTAAATAACGCATATCAGGAGTTTGTTGCTGGAGCAGCTAATGTTCTTGAAGCAAATGAAAATTTTAAACAAAAATGGGAGCTGTTCAG
GGTTGCTTGTGATCAAGCAGAGGAGTTTGTAGAGTCTGTGAAGCAAAGGATAGGATCAGAGTGCCTTGTTGATGAGGCCACTGGTTCACTGGCTGGCAAGTCTGGGCAGT
CTGGTACCACTGGCCTTCCTCCGATCAGTGCTGTTCGATTGGAACAGATGAGCAAAGCTGTAAGATGGCTCGTGATTGAGTTGCAGCACGGCTCAGGAGCTGCTGCTTCT
GCTTCAGCACACTCTCATCAGTCTGCTCCTTTTGATGCTAGATTTACTGAGGATACAACCCAATAG

mRNA sequence

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Protein sequence

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MDSLVDSLNNAYQEFVAGAANVLEANENFKQKWELFRVACDQAEEFVESVKQRIGSECLVDEATGSLAGKSGQSGTTGLPPISAVRLEQMSKAVRWLVIELQHGSGAAAS
ASAHSHQSAPFDARFTEDTTQ

IVF0003750 (gene) of Melon (IVF77) v1 genome